| Definition | Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_007348 |
| Length | 2,726,152 |
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The map label for this gene is glgX [H]
Identifier: 73538060
GI number: 73538060
Start: 874216
End: 876447
Strand: Direct
Name: glgX [H]
Synonym: Reut_B4230
Alternate gene names: 73538060
Gene position: 874216-876447 (Clockwise)
Preceding gene: 73538059
Following gene: 73538061
Centisome position: 32.07
GC content: 67.83
Gene sequence:
>2232_bases ATGAACATAGCGCTTCAGGTTCCGGACCGCCTGCTTCCCGGCAAGCCCTATCCGCTCGGCGCGCAATGGGATGGTCTCGG GGTGAACTTCGCCGTGTTCTCGGCCAATGCCTCGCGCATCGACCTGTGCCTGTTCTCGGCAAACGGACGCAAGGAACTGG CGCGCATGCCGCTGCCCGAATGCACGGACGAGATCTGGCATGGCTATCTGCCCAATGCTGCGCTTGGCACCTTGTACGGG TTCCGCGCGTTCGGCCCCTACGACCCCGCGCGCGGGCACCGCTTCAACCCGCACAAGCTGTTACTGGACCCGTACGCACG ACGACTCAGCGGGCCGGTGCGCTGGTCCGATGCCCTGTTCGGCTACCGGTTGCAGAGTGCGCGCGCCGACCTCACGCCCG ACCGCCGCGACAGCGCGCCGACCATGCCGAAGGCCGTGGTCGTCGACGAAAGCTTCCACTGGGGCGACGACCGGCCGCCC GGCGTGCCCTGGCAGGACACGGTCATCTACGAAGCCCACCTGCGCGGCGTGTCGATGCTGCGCGACGATGTGCTGCCGCA CCAGCGCGGCACGTTTGCCGCACTGTGCGACCCGCGCTTTCTCGACCATCTGCTGCGCATGGGCATCACCGCCGTCGAGC TGCTGCCGATACACGCGATCCTGCAGGACCGCTTCCTGCTGGAACGCGGCCTGCGCAATTACTGGGGCTACAACACGCTG TCGTACTTCGCGCCGGAGCCGTCGTACCTGGCGACGGACCAGCTTCACGAGCTCAAGGTCGCCGTGCGGCGCCTGCATGC CGCGGGTATCGAAGTGATCCTGGACGTGGTCTACAACCACACCTGCGAAGGCAACGAACTCGGGCCGACCGTGTCCTGGC GCGGGCTCGACAATGCCAGCTACTACCAGCTCGTGCCCGGCGACGAACGCCATTACCTCAACGACACCGGCTGCGGCAAC ATGCTGAACCTGCCCCATCCTCGCGTGCTGCAGATGGTGCTCGATTCGCTGCGCTACTGGGTGCAATGCTTCCATGTCGA CGGCTTCCGCTTCGACCTGGGTGCGACGCTGGGCCGCGACCACAACGGCTTCGACCCCGGCGCCTCGTTCTTCGACGCAA TCCTGCAGGACCCGGTGCTGAGCCGCGTCAAGCTGATCTCCGAACCGTGGGACCTGGGGCCGGGGGGATACCAGCTCGGC AACCATCCGCCCGGCTTTGCCGAGTGGAACGACAAGTTTCGCGACACCGTGCGCCGCTTCTGGCGGGGCGACTCGGGCCA GCGCGGCGAGCTCGCGGCGCGGCTGTCGGGCTCGGCCGACCTGTTCGACCGGCGCCACCGGCGGCCCTGGGCCAGCGTCA ATTTCATCACCGCGCATGACGGCTTCACGCTGGCGGACCTGGTCAGCTACAACGGCAAGCACAACGACGACAACGGCGAA GAAAACCGCGACGGGACCGACAACAACTACAGCGCCAACTGGAGCCCCGACGGCAGCGTCGAAGGCCCCACGGACGATCC CGCCATCCTGGCGCTGCGCGCCCGTGTCGCGCAAGCAATGTTGTCCACGCTGCTGCTGGCGCAGGGCACCCCGATGCTGA CCGCCGGTGACGAATGGGGCCGCAGCCAGCAGGGCAACAACAATGCGTACTGCCAGGACAACGCGATGTCGTGGCTGGAC TGGAAGCAAGCCCAGACGCCGCGCGGACAGGTGCTGCTGCAGATGGTGCGCCGCCTGCTCGCGTTGCGTCTGCGGCTGCC CTCACTCGGCGCCAACCACTTTTCCCACGGCAATCAGGAACCCGCACCGGGGATCGGCGACATTGCGTGGTTCGATACCG AGGGGCAACCACCGAGCCCGGAAGCGTGGGCCGCCCCGGAGATCCGCACGCTGGCGCTGCGCCGCGCGGCAGCGCCGCCG CGCTCGGAACGCCTCGAACTTGCCCACCGCGCGACACGAGAAGGACAGCCCGGTCCGGTCAACGTGACCCTGCTGCTGCT CAACGCCGGCGATACCGACGTCGTGTTCCGGCTACCCGAACCGGCACTCGCCTGGCAACTGGTACTGGACAGCAGCCAGC CACAGGCGGAAGCCGGACGGCCGTCGAATGCCATCACGGAGCAGGCCGTGCCTGCCCATTCGCTGGTGCTACTGGCGGCG CAGGCCGCGCCACAACGCTTCGATCATCCGGCAGAGCCCGCACGCGCCGCACCGCAGGAGGCCGCCTCATGA
Upstream 100 bases:
>100_bases TGACCGCCGAGCCGGTGCCATCGCATGGACAGGAGGCCTCCGTCTTGCTGACGCTGCCGCCGCTGGCCACGGTGATCCTG CAGCACGCCGGAACGCAGGC
Downstream 100 bases:
>100_bases GCCCCAAGACCCCCTCCGATACAGACGCATTGCATGCGCTCGCCGTGCACGTCGGCGTACAGCCACGCTGGCTCGATGCC TGGGGCCGGCCGCAACAGGT
Product: glycogen debranching protein GlgX
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 743; Mature: 743
Protein sequence:
>743_residues MNIALQVPDRLLPGKPYPLGAQWDGLGVNFAVFSANASRIDLCLFSANGRKELARMPLPECTDEIWHGYLPNAALGTLYG FRAFGPYDPARGHRFNPHKLLLDPYARRLSGPVRWSDALFGYRLQSARADLTPDRRDSAPTMPKAVVVDESFHWGDDRPP GVPWQDTVIYEAHLRGVSMLRDDVLPHQRGTFAALCDPRFLDHLLRMGITAVELLPIHAILQDRFLLERGLRNYWGYNTL SYFAPEPSYLATDQLHELKVAVRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYQLVPGDERHYLNDTGCGN MLNLPHPRVLQMVLDSLRYWVQCFHVDGFRFDLGATLGRDHNGFDPGASFFDAILQDPVLSRVKLISEPWDLGPGGYQLG NHPPGFAEWNDKFRDTVRRFWRGDSGQRGELAARLSGSADLFDRRHRRPWASVNFITAHDGFTLADLVSYNGKHNDDNGE ENRDGTDNNYSANWSPDGSVEGPTDDPAILALRARVAQAMLSTLLLAQGTPMLTAGDEWGRSQQGNNNAYCQDNAMSWLD WKQAQTPRGQVLLQMVRRLLALRLRLPSLGANHFSHGNQEPAPGIGDIAWFDTEGQPPSPEAWAAPEIRTLALRRAAAPP RSERLELAHRATREGQPGPVNVTLLLLNAGDTDVVFRLPEPALAWQLVLDSSQPQAEAGRPSNAITEQAVPAHSLVLLAA QAAPQRFDHPAEPARAAPQEAAS
Sequences:
>Translated_743_residues MNIALQVPDRLLPGKPYPLGAQWDGLGVNFAVFSANASRIDLCLFSANGRKELARMPLPECTDEIWHGYLPNAALGTLYG FRAFGPYDPARGHRFNPHKLLLDPYARRLSGPVRWSDALFGYRLQSARADLTPDRRDSAPTMPKAVVVDESFHWGDDRPP GVPWQDTVIYEAHLRGVSMLRDDVLPHQRGTFAALCDPRFLDHLLRMGITAVELLPIHAILQDRFLLERGLRNYWGYNTL SYFAPEPSYLATDQLHELKVAVRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYQLVPGDERHYLNDTGCGN MLNLPHPRVLQMVLDSLRYWVQCFHVDGFRFDLGATLGRDHNGFDPGASFFDAILQDPVLSRVKLISEPWDLGPGGYQLG NHPPGFAEWNDKFRDTVRRFWRGDSGQRGELAARLSGSADLFDRRHRRPWASVNFITAHDGFTLADLVSYNGKHNDDNGE ENRDGTDNNYSANWSPDGSVEGPTDDPAILALRARVAQAMLSTLLLAQGTPMLTAGDEWGRSQQGNNNAYCQDNAMSWLD WKQAQTPRGQVLLQMVRRLLALRLRLPSLGANHFSHGNQEPAPGIGDIAWFDTEGQPPSPEAWAAPEIRTLALRRAAAPP RSERLELAHRATREGQPGPVNVTLLLLNAGDTDVVFRLPEPALAWQLVLDSSQPQAEAGRPSNAITEQAVPAHSLVLLAA QAAPQRFDHPAEPARAAPQEAAS >Mature_743_residues MNIALQVPDRLLPGKPYPLGAQWDGLGVNFAVFSANASRIDLCLFSANGRKELARMPLPECTDEIWHGYLPNAALGTLYG FRAFGPYDPARGHRFNPHKLLLDPYARRLSGPVRWSDALFGYRLQSARADLTPDRRDSAPTMPKAVVVDESFHWGDDRPP GVPWQDTVIYEAHLRGVSMLRDDVLPHQRGTFAALCDPRFLDHLLRMGITAVELLPIHAILQDRFLLERGLRNYWGYNTL SYFAPEPSYLATDQLHELKVAVRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYQLVPGDERHYLNDTGCGN MLNLPHPRVLQMVLDSLRYWVQCFHVDGFRFDLGATLGRDHNGFDPGASFFDAILQDPVLSRVKLISEPWDLGPGGYQLG NHPPGFAEWNDKFRDTVRRFWRGDSGQRGELAARLSGSADLFDRRHRRPWASVNFITAHDGFTLADLVSYNGKHNDDNGE ENRDGTDNNYSANWSPDGSVEGPTDDPAILALRARVAQAMLSTLLLAQGTPMLTAGDEWGRSQQGNNNAYCQDNAMSWLD WKQAQTPRGQVLLQMVRRLLALRLRLPSLGANHFSHGNQEPAPGIGDIAWFDTEGQPPSPEAWAAPEIRTLALRRAAAPP RSERLELAHRATREGQPGPVNVTLLLLNAGDTDVVFRLPEPALAWQLVLDSSQPQAEAGRPSNAITEQAVPAHSLVLLAA QAAPQRFDHPAEPARAAPQEAAS
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=665, Percent_Identity=47.218045112782, Blast_Score=558, Evalue=1e-160, Organism=Saccharomyces cerevisiae, GI6320826, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=3e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013780 - InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR011837 - InterPro: IPR013783 - InterPro: IPR014756 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]
EC number: 3.2.1.- [C]
Molecular weight: Translated: 82543; Mature: 82543
Theoretical pI: Translated: 6.23; Mature: 6.23
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIALQVPDRLLPGKPYPLGAQWDGLGVNFAVFSANASRIDLCLFSANGRKELARMPLPE CCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCHH CTDEIWHGYLPNAALGTLYGFRAFGPYDPARGHRFNPHKLLLDPYARRLSGPVRWSDALF HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHH GYRLQSARADLTPDRRDSAPTMPKAVVVDESFHWGDDRPPGVPWQDTVIYEAHLRGVSML HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH RDDVLPHQRGTFAALCDPRFLDHLLRMGITAVELLPIHAILQDRFLLERGLRNYWGYNTL HHHCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE SYFAPEPSYLATDQLHELKVAVRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNAS EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC YYQLVPGDERHYLNDTGCGNMLNLPHPRVLQMVLDSLRYWVQCFHVDGFRFDLGATLGRD EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC HNGFDPGASFFDAILQDPVLSRVKLISEPWDLGPGGYQLGNHPPGFAEWNDKFRDTVRRF CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH WRGDSGQRGELAARLSGSADLFDRRHRRPWASVNFITAHDGFTLADLVSYNGKHNDDNGE HCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCC ENRDGTDNNYSANWSPDGSVEGPTDDPAILALRARVAQAMLSTLLLAQGTPMLTAGDEWG CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHC RSQQGNNNAYCQDNAMSWLDWKQAQTPRGQVLLQMVRRLLALRLRLPSLGANHFSHGNQE CCCCCCCCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC PAPGIGDIAWFDTEGQPPSPEAWAAPEIRTLALRRAAAPPRSERLELAHRATREGQPGPV CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE NVTLLLLNAGDTDVVFRLPEPALAWQLVLDSSQPQAEAGRPSNAITEQAVPAHSLVLLAA EEEEEEEECCCCEEEEECCCHHHEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH QAAPQRFDHPAEPARAAPQEAAS HHCHHHCCCCCCCHHCCCHHCCC >Mature Secondary Structure MNIALQVPDRLLPGKPYPLGAQWDGLGVNFAVFSANASRIDLCLFSANGRKELARMPLPE CCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCHH CTDEIWHGYLPNAALGTLYGFRAFGPYDPARGHRFNPHKLLLDPYARRLSGPVRWSDALF HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHH GYRLQSARADLTPDRRDSAPTMPKAVVVDESFHWGDDRPPGVPWQDTVIYEAHLRGVSML HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH RDDVLPHQRGTFAALCDPRFLDHLLRMGITAVELLPIHAILQDRFLLERGLRNYWGYNTL HHHCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE SYFAPEPSYLATDQLHELKVAVRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNAS EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC YYQLVPGDERHYLNDTGCGNMLNLPHPRVLQMVLDSLRYWVQCFHVDGFRFDLGATLGRD EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC HNGFDPGASFFDAILQDPVLSRVKLISEPWDLGPGGYQLGNHPPGFAEWNDKFRDTVRRF CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH WRGDSGQRGELAARLSGSADLFDRRHRRPWASVNFITAHDGFTLADLVSYNGKHNDDNGE HCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCC ENRDGTDNNYSANWSPDGSVEGPTDDPAILALRARVAQAMLSTLLLAQGTPMLTAGDEWG CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHC RSQQGNNNAYCQDNAMSWLDWKQAQTPRGQVLLQMVRRLLALRLRLPSLGANHFSHGNQE CCCCCCCCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC PAPGIGDIAWFDTEGQPPSPEAWAAPEIRTLALRRAAAPPRSERLELAHRATREGQPGPV CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE NVTLLLLNAGDTDVVFRLPEPALAWQLVLDSSQPQAEAGRPSNAITEQAVPAHSLVLLAA EEEEEEEECCCCEEEEECCCHHHEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH QAAPQRFDHPAEPARAAPQEAAS HHCHHHCCCCCCCHHCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Hydrolase; Glycosylases; Glycosidases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]