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The map label for this gene is glgB

Identifier: 73538059

GI number: 73538059

Start: 871967

End: 874219

Strand: Direct

Name: glgB

Synonym: Reut_B4229

Alternate gene names: 73538059

Gene position: 871967-874219 (Clockwise)

Preceding gene: 73538058

Following gene: 73538060

Centisome position: 31.99

GC content: 68.0

Gene sequence:

>2253_bases
ATGAACCGAGTCGACACCCGGCCGCCCGAGCGACGGACGCAAACCATATCCGACGGCGAGTTGGGCGCGCTGGTTGAAGG
TCGGCATCGCGATCCGTTTGCGATTCTGGGCCCGCATCGCGACGGCGATACGCTGATCGTGCGCGCCTGCGTGCCCGGGG
CGCACTCCGTGATGCTGGCCGATTCGCGGGGTGAACCGCTGGCCCCCATGACGCCGCTGCACGCGGGCGGCGTGTTCACC
GGCCGCCTGCCCGCCGGGGTCTCCGTCTACCAGCTCCTGGTCCGCTGGCACAACGGCACTCAGCAGGTGAGCCACGATCC
TTATGCGTTCGGACTGCTGCTGGGCGAGCTGGACCTGCACCTCATTGCCGAAGGCCGGCACTTCGAGCTTGGGGCCTGTC
TTGGCGCACAGTGGCGCAACGTGGACGGCGTCCAGGGTGTGCGCTTTGCGGTGTGGGCGCCCAACGCCCGGCGCGTGTCG
GTCATCGGCGATTTCAACGGCTGGCAGCCCGCGCGGCATCCCATGCGGCTGCGCCACCCGAGCGGCGTCTGGGAACTCTT
CATTCCCGAAGCCATGGGCGCGCGGCCCGGCTGCCGCTACAAGTTCGACTTGCTGGACCCGCATGACGCGCAATTGCCCG
ACAAGGCCGACCCGCTCGCGCTGGCGACCGAGGCGCCGCCAGCAACGGCATCGGTCGTGACGCAGCCGCAGGTTTCCGCG
CCTCCGTTCGCGTGGCAGGACGATGAGTGGATGCGCCTGCGCAATGCCGTCGATCCGTATGCGGCGCCGCTGTCGATCTA
CGAGGTCCACGTCGGCTCCTGGCTGCGCGCGGCCAACGACCCGGCACGCGGCTGGGAGGTGCTGGCCGACCGGCTCATCC
CCTATGTCCACGAACTGGGCTTCACGCACATCGAACTGCTGCCCGTGACGGAGCACCCGTTCGGCGGCTCGTGGGGCTAC
CAGCCGCTGTCGCTCTACGCGCCCACGGCGCGACTGGGACCCCCGCAGGCGTTCGCGGCCTTTATCGATCGCTGTCACCG
CGACGGCATCGGCGTCATCCTCGATTGGGTACCCGCGCATTTCCCGACGGATCCACACGGGCTGGCGCGCTTCGATGGCA
CCGCGCTCTACGAGCACGAAGACCCGCGCGAAGGCTTCCACCAGGACTGGAATACGCTGATCTACAACCTTGGGCGCAAC
GAAGTGCGCGGCTTCCTGCTGGCGGGCGCGCTGCACTGGCTCGAACACTTCCACGTCGACGGCCTGCGCGTCGATGCGGT
GGCCTCCATGCTCTACCGCGACTACAGCCGCGCCCCCGACCAATGGGTGCCTAACCGCTTCGGCGGACGCGAGAACCTGG
AGGCAGTCGCCTTCCTGCGCGAACTCAACACCGTCGTGCATGAACGCTGCCCCGGCGCGCTCACCATTGCCGAGGAATCC
ACAGCGTGGCCTGGCGTGACAGCCAGCGCGGCCAGCGGCGGGCTCGGCTTCGACTTCAAGTGGAATATGGGCTGGATGCA
CGACACGCTGCGCTACCTGTCGCTGGATCCGATTCACCGCGCGTGGCACCACCAGGACATGACGTTCGGCACCGTCTACG
CGTGGTCGGAAGCGTTCGTGCTGCCGCTGTCGCACGACGAAGTCGTGCACGGCAAAGGCTCGATGCTGCGCAAGTGCCCG
GGCGACGACTGGCAGCGCTTCGCCGGACTGCGCGCCTACTACGGCTTCATGTGGGCCCATCCGGGCAAGAAGCTGCTGTT
CATGGGCGGTGAACTGGCCCAGTGGCAGGAATGGAACCACGACGCCGAGCTCGACTGGGCACTGCTCGACCATCCGATGC
ACCGCGGCGTCCACACGCTGGTGCGCGACCTCAATGCGCTCTACCGGGAGTTGCCTGCCTTGCATGAACTCGACCATTCG
CCCGCCGGCTTCCAGTGGGTAGTCGGCGACGATCACCAGAACAGTGTATTCGCGTGGCTGCGCCGTCCGGCGCCCGGCAG
CGGAGACGTCGTGCTGGCCGTCACGAATATGACGCCAGTGCCGCGGTACGGCTACCGGATCGGCGTGCCGTCCGAAGGCT
GCTGGCATGAGCGGCTCAACACCGACGCGGCGTGTTACGGCGGCAGCAACCTGGGCAATGGCGGTGCCGTGACCGCCGAG
CCGGTGCCATCGCATGGACAGGAGGCCTCCGTCTTGCTGACGCTGCCGCCGCTGGCCACGGTGATCCTGCAGCACGCCGG
AACGCAGGCATGA

Upstream 100 bases:

>100_bases
TCGAATATGAAGCGGCCAATCGCGTCGCCTGGATCGACCTTCCGGCCAGCGGGCTCGCGCGCATGGTGCGCAAGCTCATG
GCCGGAAACCTGGAGGCGCA

Downstream 100 bases:

>100_bases
ACATAGCGCTTCAGGTTCCGGACCGCCTGCTTCCCGGCAAGCCCTATCCGCTCGGCGCGCAATGGGATGGTCTCGGGGTG
AACTTCGCCGTGTTCTCGGC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLADSRGEPLAPMTPLHAGGVFT
GRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLHLIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVS
VIGDFNGWQPARHPMRLRHPSGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA
PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELGFTHIELLPVTEHPFGGSWGY
QPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRN
EVRGFLLAGALHWLEHFHVDGLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES
TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFVLPLSHDEVVHGKGSMLRKCP
GDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHS
PAGFQWVVGDDHQNSVFAWLRRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE
PVPSHGQEASVLLTLPPLATVILQHAGTQA

Sequences:

>Translated_750_residues
MNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLADSRGEPLAPMTPLHAGGVFT
GRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLHLIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVS
VIGDFNGWQPARHPMRLRHPSGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA
PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELGFTHIELLPVTEHPFGGSWGY
QPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRN
EVRGFLLAGALHWLEHFHVDGLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES
TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFVLPLSHDEVVHGKGSMLRKCP
GDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHS
PAGFQWVVGDDHQNSVFAWLRRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE
PVPSHGQEASVLLTLPPLATVILQHAGTQA
>Mature_750_residues
MNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLADSRGEPLAPMTPLHAGGVFT
GRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLHLIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVS
VIGDFNGWQPARHPMRLRHPSGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA
PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELGFTHIELLPVTEHPFGGSWGY
QPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRN
EVRGFLLAGALHWLEHFHVDGLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES
TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFVLPLSHDEVVHGKGSMLRKCP
GDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHS
PAGFQWVVGDDHQNSVFAWLRRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE
PVPSHGQEASVLLTLPPLATVILQHAGTQA

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=656, Percent_Identity=27.2865853658537, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1789839, Length=737, Percent_Identity=53.4599728629579, Blast_Score=765, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=351, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554896, Length=380, Percent_Identity=28.4210526315789, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32564391, Length=294, Percent_Identity=29.9319727891156, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320826, Length=636, Percent_Identity=25.9433962264151, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28573410, Length=612, Percent_Identity=26.9607843137255, Blast_Score=175, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_CUPPJ (Q46TF2)

Other databases:

- EMBL:   CP000091
- RefSeq:   YP_298426.1
- ProteinModelPortal:   Q46TF2
- GeneID:   3612659
- GenomeReviews:   CP000091_GR
- KEGG:   reu:Reut_B4229
- NMPDR:   fig|264198.3.peg.5463
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- ProtClustDB:   PRK05402
- BioCyc:   REUT264198:REUT_B4229-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 83329; Mature: 83329

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: ACT_SITE 320-320 ACT_SITE 355-355 ACT_SITE 360-360 ACT_SITE 423-423 ACT_SITE 425-425 ACT_SITE 478-478 ACT_SITE 545-545 ACT_SITE 546-546

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLA
CCCCCCCCCHHHHCCCCCCCCCCEECCCCCCCEEEECCCCCCCEEEEEEECCCCCEEEEE
DSRGEPLAPMTPLHAGGVFTGRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLH
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEE
LIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVSVIGDFNGWQPARHPMRLRHP
EEECCCEEEEEHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEECCC
SGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA
CCCEEEECCHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCC
PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELG
CCCCCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
FTHIELLPVTEHPFGGSWGYQPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAH
CCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
FPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNEVRGFLLAGALHWLEHFHVD
CCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFV
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCEEE
LPLSHDEVVHGKGSMLRKCPGDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNH
EECCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHHCCC
DAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHSPAGFQWVVGDDHQNSVFAWL
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCCCEEEEE
RRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE
ECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHCCCCEEEECCCCCCCCCEEECC
PVPSHGQEASVLLTLPPLATVILQHAGTQA
CCCCCCCCCEEEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLA
CCCCCCCCCHHHHCCCCCCCCCCEECCCCCCCEEEECCCCCCCEEEEEEECCCCCEEEEE
DSRGEPLAPMTPLHAGGVFTGRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLH
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEE
LIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVSVIGDFNGWQPARHPMRLRHP
EEECCCEEEEEHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEECCC
SGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA
CCCEEEECCHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCC
PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELG
CCCCCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
FTHIELLPVTEHPFGGSWGYQPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAH
CCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
FPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNEVRGFLLAGALHWLEHFHVD
CCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFV
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCEEE
LPLSHDEVVHGKGSMLRKCPGDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNH
EECCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHHCCC
DAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHSPAGFQWVVGDDHQNSVFAWL
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCCCEEEEE
RRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE
ECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHCCCCEEEECCCCCCCCCEEECC
PVPSHGQEASVLLTLPPLATVILQHAGTQA
CCCCCCCCCEEEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA