Click here to switch to the map view.

The map label for this gene is mhpA [H]

Identifier: 73538048

GI number: 73538048

Start: 858272

End: 860011

Strand: Direct

Name: mhpA [H]

Synonym: Reut_B4218

Alternate gene names: 73538048

Gene position: 858272-860011 (Clockwise)

Preceding gene: 73538047

Following gene: 73538049

Centisome position: 31.48

GC content: 66.44

Gene sequence:

>1740_bases
ATGACCATCGCCAGCGCCATTGATTACCAGCGCCTGTCATTCGAGTACCAGCCGTGCGCGGACCAGCGCGACGGGCAAGC
GGGCGGCGCGGTCCATCCTGTCGTCGTGGTCGGTGCCGGGCCCATCGGCCTGGCCACGGCCATCGACCTGGCGCAGCGCG
GCGTGCGCGTGGTGCTGGTCGATGACGACTGCACGCTGTCAACGGGATCGCGCGCGATCTGCTTCGCCAAGCGGACGCTG
GATATCTTCGACCGCCTGGGCTGCGGCCAGCGCATGGTGGATACGGGCGTGAGCTGGAACGTCGGCCGCGTCTTCCTGCG
CGACCAGCAGGTCTACAGCTTCGATCTGTTGCCCGAAGCGGGCCATCACCGCCCCGCTTTCATCAACCTGCAGCAGTATT
ACGTAGAAGGTTACCTGCTTGAACGCGCGCAGGAACTTCCCAATATCGAGATCCGGTGGCGCAACAAGGTCGTCGGGCTC
GAACAGCGCCAGCCCGGCACGCCCGAAGAGACGGTCGCGCTGACCATCGAGACGCCGGACGGTTGCTACACGCTCGCTGC
CCGCTACGTAGTCGCAGCCGACGGCTCGCGCAGCCCGGTGCGCAAGCTGCTGGGCCTGGACAGCAAGGGCCGGACCTTCA
AGGACCGCTTCCTGATTGCCGATGTGAAGATGGAGGCTGACTTCCCCGCCGAACGCTGGTTCTGGTTCGACCCGCCGTTC
CACCCGCATCAGTCTGTGCTGCTGCACCGCCAGCCCGACAACGTCTGGCGCATCGACTTCCAGCTCGGCTGGGACGCCGA
TCCCGTGCTGGAGAAAACGCCGGAACGCGTGATTCCGCGTGTGCAGGCGCTGCTCGGCCCGGATGCGAAATTCACGCTCG
AGTGGGTCAGCGTCTACACGTTCTCGTGCCTGCGCATGGACAGCTTCCGGCATGGCAACGTGCTGTTTGCCGGCGATTCC
GCGCACGGTGTCTCGCCGTTCGGTGCGCGCGGCGCCAACAGCGGCGTGCAGGATGCCGAGAACCTCGCGTGGAAGCTCGC
TTATGTGCTGGACGGCCGCGCCGCGGACAGCCTGCTCGATACTTACGCGAGCGAACGCGAGTACGCAGCCGACGAGAACA
TCCTGAACTCCACCCGCTCGACCGATTTCATCACGCCCAAGAGCGAGGTCAGCCGCGTATTCCGCGATGCGGTGCTGGGC
CTGGCCCGCCGCCACGCGTTTGCGCGCAAGCTGGTCAACAGCGGCCGCCTGTCAGTGCCCGCGGTGCTGCACGGCTCGCC
GCTCAACACGCCGGATGTCGATGGCTTCGGCGACACGATGGTGCCGGGCACGGCTTGCGTGGATGCCCCCGTTTCCGACG
CGCATGGTGCAGGTTGGCTGCTCCAGCATCTCGGTGGGGAATTCACGGGGCTGGTGTTCGGCTCGCCCGACAGCCTCGAC
GGCGATAGCCGCCTTGCGCTCGAACAGATCGAACAGTCGGGCCTGCCGCTGCGCATCGTGTACCTCGGCACATCGACGCA
AACCGTGTCCGGTACCGCCATTCTGCGTGACGACAAAGGTCTTGCGGCTCAGCGTTACAACGCCGCGCCGGGCACCTTCT
ACCTGATCCGGCCGGACCAGCATGTCTGCGCACGCTGGCGCCGCATCGATGCGCAGCGTATCCGCGACGCACTGGCGCGC
GCAAGCGGTGCGCAATTGCCCGCGAGCAAGGCCCTTGAATCGGCCGCGATGGCGGCATAA

Upstream 100 bases:

>100_bases
TGGACGGCCCAGCGCGACAAGGAAATGTGGGCGGCTCTGCAGGACTGACGCGCAGACGATACATACAAGAGACTGAACGA
GAGTGGACGGAGACATAGGC

Downstream 100 bases:

>100_bases
GCCAACCCGCCGCCATACAGAATTCGGAGACATCGCCATGCCGGCCCACGCCCAAGACACCCAGGCGCACACGCTCAACA
CCGAGCCCAACCTCGCACGT

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTIASAIDYQRLSFEYQPCADQRDGQAGGAVHPVVVVGAGPIGLATAIDLAQRGVRVVLVDDDCTLSTGSRAICFAKRTL
DIFDRLGCGQRMVDTGVSWNVGRVFLRDQQVYSFDLLPEAGHHRPAFINLQQYYVEGYLLERAQELPNIEIRWRNKVVGL
EQRQPGTPEETVALTIETPDGCYTLAARYVVAADGSRSPVRKLLGLDSKGRTFKDRFLIADVKMEADFPAERWFWFDPPF
HPHQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPDAKFTLEWVSVYTFSCLRMDSFRHGNVLFAGDS
AHGVSPFGARGANSGVQDAENLAWKLAYVLDGRAADSLLDTYASEREYAADENILNSTRSTDFITPKSEVSRVFRDAVLG
LARRHAFARKLVNSGRLSVPAVLHGSPLNTPDVDGFGDTMVPGTACVDAPVSDAHGAGWLLQHLGGEFTGLVFGSPDSLD
GDSRLALEQIEQSGLPLRIVYLGTSTQTVSGTAILRDDKGLAAQRYNAAPGTFYLIRPDQHVCARWRRIDAQRIRDALAR
ASGAQLPASKALESAAMAA

Sequences:

>Translated_579_residues
MTIASAIDYQRLSFEYQPCADQRDGQAGGAVHPVVVVGAGPIGLATAIDLAQRGVRVVLVDDDCTLSTGSRAICFAKRTL
DIFDRLGCGQRMVDTGVSWNVGRVFLRDQQVYSFDLLPEAGHHRPAFINLQQYYVEGYLLERAQELPNIEIRWRNKVVGL
EQRQPGTPEETVALTIETPDGCYTLAARYVVAADGSRSPVRKLLGLDSKGRTFKDRFLIADVKMEADFPAERWFWFDPPF
HPHQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPDAKFTLEWVSVYTFSCLRMDSFRHGNVLFAGDS
AHGVSPFGARGANSGVQDAENLAWKLAYVLDGRAADSLLDTYASEREYAADENILNSTRSTDFITPKSEVSRVFRDAVLG
LARRHAFARKLVNSGRLSVPAVLHGSPLNTPDVDGFGDTMVPGTACVDAPVSDAHGAGWLLQHLGGEFTGLVFGSPDSLD
GDSRLALEQIEQSGLPLRIVYLGTSTQTVSGTAILRDDKGLAAQRYNAAPGTFYLIRPDQHVCARWRRIDAQRIRDALAR
ASGAQLPASKALESAAMAA
>Mature_578_residues
TIASAIDYQRLSFEYQPCADQRDGQAGGAVHPVVVVGAGPIGLATAIDLAQRGVRVVLVDDDCTLSTGSRAICFAKRTLD
IFDRLGCGQRMVDTGVSWNVGRVFLRDQQVYSFDLLPEAGHHRPAFINLQQYYVEGYLLERAQELPNIEIRWRNKVVGLE
QRQPGTPEETVALTIETPDGCYTLAARYVVAADGSRSPVRKLLGLDSKGRTFKDRFLIADVKMEADFPAERWFWFDPPFH
PHQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPDAKFTLEWVSVYTFSCLRMDSFRHGNVLFAGDSA
HGVSPFGARGANSGVQDAENLAWKLAYVLDGRAADSLLDTYASEREYAADENILNSTRSTDFITPKSEVSRVFRDAVLGL
ARRHAFARKLVNSGRLSVPAVLHGSPLNTPDVDGFGDTMVPGTACVDAPVSDAHGAGWLLQHLGGEFTGLVFGSPDSLDG
DSRLALEQIEQSGLPLRIVYLGTSTQTVSGTAILRDDKGLAAQRYNAAPGTFYLIRPDQHVCARWRRIDAQRIRDALARA
SGAQLPASKALESAAMAA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=524, Percent_Identity=25.9541984732824, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 63549; Mature: 63418

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIASAIDYQRLSFEYQPCADQRDGQAGGAVHPVVVVGAGPIGLATAIDLAQRGVRVVLV
CCCCHHCCHHHHCEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEE
DDDCTLSTGSRAICFAKRTLDIFDRLGCGQRMVDTGVSWNVGRVFLRDQQVYSFDLLPEA
CCCCCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCEEEEEECCCCC
GHHRPAFINLQQYYVEGYLLERAQELPNIEIRWRNKVVGLEQRQPGTPEETVALTIETPD
CCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCEEEEEEECCC
GCYTLAARYVVAADGSRSPVRKLLGLDSKGRTFKDRFLIADVKMEADFPAERWFWFDPPF
CCEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEECEEEEEEEEECCCCCHHCEEEECCCC
HPHQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPDAKFTLEWVSVYT
CCCCCEEEEECCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHH
FSCLRMDSFRHGNVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAYVLDGRAADSLLD
HHHHEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCHHHHHHH
TYASEREYAADENILNSTRSTDFITPKSEVSRVFRDAVLGLARRHAFARKLVNSGRLSVP
HHHHCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AVLHGSPLNTPDVDGFGDTMVPGTACVDAPVSDAHGAGWLLQHLGGEFTGLVFGSPDSLD
EEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCC
GDSRLALEQIEQSGLPLRIVYLGTSTQTVSGTAILRDDKGLAAQRYNAAPGTFYLIRPDQ
CCCHHHHHHHHHCCCCEEEEEECCCCCEECCEEEEECCCCCCHHCCCCCCCEEEEECCCH
HVCARWRRIDAQRIRDALARASGAQLPASKALESAAMAA
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TIASAIDYQRLSFEYQPCADQRDGQAGGAVHPVVVVGAGPIGLATAIDLAQRGVRVVLV
CCCHHCCHHHHCEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEE
DDDCTLSTGSRAICFAKRTLDIFDRLGCGQRMVDTGVSWNVGRVFLRDQQVYSFDLLPEA
CCCCCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCEEEEEECCCCC
GHHRPAFINLQQYYVEGYLLERAQELPNIEIRWRNKVVGLEQRQPGTPEETVALTIETPD
CCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCEEEEEEECCC
GCYTLAARYVVAADGSRSPVRKLLGLDSKGRTFKDRFLIADVKMEADFPAERWFWFDPPF
CCEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEECEEEEEEEEECCCCCHHCEEEECCCC
HPHQSVLLHRQPDNVWRIDFQLGWDADPVLEKTPERVIPRVQALLGPDAKFTLEWVSVYT
CCCCCEEEEECCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHH
FSCLRMDSFRHGNVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAYVLDGRAADSLLD
HHHHEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCHHHHHHH
TYASEREYAADENILNSTRSTDFITPKSEVSRVFRDAVLGLARRHAFARKLVNSGRLSVP
HHHHCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AVLHGSPLNTPDVDGFGDTMVPGTACVDAPVSDAHGAGWLLQHLGGEFTGLVFGSPDSLD
EEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCC
GDSRLALEQIEQSGLPLRIVYLGTSTQTVSGTAILRDDKGLAAQRYNAAPGTFYLIRPDQ
CCCHHHHHHHHHCCCCEEEEEECCCCCEECCEEEEECCCCCCHHCCCCCCCEEEEECCCH
HVCARWRRIDAQRIRDALARASGAQLPASKALESAAMAA
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA