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The map label for this gene is czcS [H]

Identifier: 73537795

GI number: 73537795

Start: 595413

End: 596855

Strand: Direct

Name: czcS [H]

Synonym: Reut_B3962

Alternate gene names: 73537795

Gene position: 595413-596855 (Clockwise)

Preceding gene: 73537794

Following gene: 73537796

Centisome position: 21.84

GC content: 69.09

Gene sequence:

>1443_bases
ATGCGGTTGCCATCGTCGCTTACCGCGCGCCTGACGCTCGCATATGGACTGGCGACTGCCGCGATCCTGGCGGGCGTCGC
CGCATACCTGTCGAGCGCACTGTCGGCGCAGTTGCAGGGCCGTGACGAAAGTGAGCTGGTCAGCGAGGTGCTGCTCGTGC
GCCATCTGCTGCGCGAGGTGCGCAACGAAGACGAGGTCCGCGCCGACACGCATCGCTTTGCGGACATCGCCATGGGCCAT
GAAGGCCTGATCCTGGTCGTGCGCACGCCGCAGGGCGATCCGTTGGTCACGCTCAATCCGCGCAACGAAGCCATTCCGCC
GCTGCACGTGCTGCCGGTCACCACGCAGCCGCACAAGTCGATGCTGGAAGCCTGGCGGCCGAAGAGCGGGGCGCAGTCGC
CGGCCATTGCGGCGCTTGGCCAGCTTGGCGACACCGGCCGCTCGGTCGAGATCGTGGTGCTGCGCGACGCCGGTTACCGC
GTGGCGCTGATGCGCGAGTACCGTCATCGGTTGCTTGCGGCCACGCTGTGTGGTGCGGCAGTCGCGGCGGCGCTCGGCTT
TGTGCTCGCACGGCGCGGGCTGCGACCGCTGCGCCGCATGGCGGCCGATGCGGCGGCGGTGACCACGAGCCGCCTTGCCA
CGCGTCTCGATGCCGGCAGCGCGCCGGCCGAACTGCGCGAACTGGCGCGCGAACTCAACGGCATGCTGGCGCGCCTGCAG
GACAGCTTCTCGCGGCTGTCCGCGTTCTCGGCGGACCTTGCGCACGACTTCCGCACGCCCATCAGCAATCTTGTCGGCCA
GACCGAAGTCACGCTCGCGCAGAGCCGCAGCGTGGCGGAGTACGAGGCATTGCTCGAATCCAATCTCGAGGAATACGAGC
GCCTGTCGCGCATGATCGAAAACATGCTGTTCCTCGCGCGTGCCGACCACGCCCAGGTCGCCATGGGCGTGCGTGGGCTG
GATGCGCGCGCGGAGCTCGACAAGGTAGCCGAGTATTTCGAGGCCGTGGCCGCCGACCGCAACGTGCGCCTGTCGGTGGC
GGGCGATGCAAGCATCCACGCGGACCAGACGCTACTGCGTCGTGCCGTCACGAACCTGCTCGACAATGCCCTGCGCCATG
CCCCTGCTGGCAGCACGGTACGCGTGGACATCGCGCGGCGCGCCGGCGAAGTTGGCGAAGCGGGCGATGGCACGTGCATC
CGCGTGGTCAATGCGGGCCCGGCCATCGCCCCCGAGGTCTTGCCGCACATCTTCGGCCGCTTCTACCGTGCCGATCCGTC
GCGGCGCAATTCCGCGGCATCCACGGGGCTGGGACTGGCCATCGTCGATTCCATCATGCGCCTGCACGGCGGGCGGGCGC
TCGTCAGCAGCAGCGACGGCGAGACCGTGTTTTCGCTGCTGTTCCCCGCTGGTGGCGACCCGCGTCCCCACGCAGTCGCC
TGA

Upstream 100 bases:

>100_bases
GCGCGAAGGTCGACGATCCGTTCGACCACAAGCTGATCCACACGCGGCGCGGCATGGGCTACGTGCTCGATGATGGCGAG
CCCGGACTGGGCGACGCGTG

Downstream 100 bases:

>100_bases
ATCCATGGCGGCTTGCCAAGTCGCCGTACGAGGCCTATCTTGCGGGCATCCCGGTCCGTTGCGGCCGGGGCCAACTTGGT
CGTTCCATCTCTTGCAAGGA

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MRLPSSLTARLTLAYGLATAAILAGVAAYLSSALSAQLQGRDESELVSEVLLVRHLLREVRNEDEVRADTHRFADIAMGH
EGLILVVRTPQGDPLVTLNPRNEAIPPLHVLPVTTQPHKSMLEAWRPKSGAQSPAIAALGQLGDTGRSVEIVVLRDAGYR
VALMREYRHRLLAATLCGAAVAAALGFVLARRGLRPLRRMAADAAAVTTSRLATRLDAGSAPAELRELARELNGMLARLQ
DSFSRLSAFSADLAHDFRTPISNLVGQTEVTLAQSRSVAEYEALLESNLEEYERLSRMIENMLFLARADHAQVAMGVRGL
DARAELDKVAEYFEAVAADRNVRLSVAGDASIHADQTLLRRAVTNLLDNALRHAPAGSTVRVDIARRAGEVGEAGDGTCI
RVVNAGPAIAPEVLPHIFGRFYRADPSRRNSAASTGLGLAIVDSIMRLHGGRALVSSSDGETVFSLLFPAGGDPRPHAVA

Sequences:

>Translated_480_residues
MRLPSSLTARLTLAYGLATAAILAGVAAYLSSALSAQLQGRDESELVSEVLLVRHLLREVRNEDEVRADTHRFADIAMGH
EGLILVVRTPQGDPLVTLNPRNEAIPPLHVLPVTTQPHKSMLEAWRPKSGAQSPAIAALGQLGDTGRSVEIVVLRDAGYR
VALMREYRHRLLAATLCGAAVAAALGFVLARRGLRPLRRMAADAAAVTTSRLATRLDAGSAPAELRELARELNGMLARLQ
DSFSRLSAFSADLAHDFRTPISNLVGQTEVTLAQSRSVAEYEALLESNLEEYERLSRMIENMLFLARADHAQVAMGVRGL
DARAELDKVAEYFEAVAADRNVRLSVAGDASIHADQTLLRRAVTNLLDNALRHAPAGSTVRVDIARRAGEVGEAGDGTCI
RVVNAGPAIAPEVLPHIFGRFYRADPSRRNSAASTGLGLAIVDSIMRLHGGRALVSSSDGETVFSLLFPAGGDPRPHAVA
>Mature_480_residues
MRLPSSLTARLTLAYGLATAAILAGVAAYLSSALSAQLQGRDESELVSEVLLVRHLLREVRNEDEVRADTHRFADIAMGH
EGLILVVRTPQGDPLVTLNPRNEAIPPLHVLPVTTQPHKSMLEAWRPKSGAQSPAIAALGQLGDTGRSVEIVVLRDAGYR
VALMREYRHRLLAATLCGAAVAAALGFVLARRGLRPLRRMAADAAAVTTSRLATRLDAGSAPAELRELARELNGMLARLQ
DSFSRLSAFSADLAHDFRTPISNLVGQTEVTLAQSRSVAEYEALLESNLEEYERLSRMIENMLFLARADHAQVAMGVRGL
DARAELDKVAEYFEAVAADRNVRLSVAGDASIHADQTLLRRAVTNLLDNALRHAPAGSTVRVDIARRAGEVGEAGDGTCI
RVVNAGPAIAPEVLPHIFGRFYRADPSRRNSAASTGLGLAIVDSIMRLHGGRALVSSSDGETVFSLLFPAGGDPRPHAVA

Specific function: Member of the two-component regulatory system CzcS/CzcR involved in the control of cobalt, zinc and cadmium homeostasis. Probably activates CzcR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=309, Percent_Identity=41.4239482200647, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788279, Length=466, Percent_Identity=29.8283261802575, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.2511210762332, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789403, Length=313, Percent_Identity=30.9904153354633, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=268, Percent_Identity=25.7462686567164, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787894, Length=314, Percent_Identity=30.2547770700637, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=290, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=306, Percent_Identity=25.8169934640523, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790436, Length=250, Percent_Identity=26, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=25.974025974026, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=242, Percent_Identity=26.4462809917355, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51437; Mature: 51437

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPSSLTARLTLAYGLATAAILAGVAAYLSSALSAQLQGRDESELVSEVLLVRHLLREV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RNEDEVRADTHRFADIAMGHEGLILVVRTPQGDPLVTLNPRNEAIPPLHVLPVTTQPHKS
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCHHH
MLEAWRPKSGAQSPAIAALGQLGDTGRSVEIVVLRDAGYRVALMREYRHRLLAATLCGAA
HHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VAAALGFVLARRGLRPLRRMAADAAAVTTSRLATRLDAGSAPAELRELARELNGMLARLQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DSFSRLSAFSADLAHDFRTPISNLVGQTEVTLAQSRSVAEYEALLESNLEEYERLSRMIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
NMLFLARADHAQVAMGVRGLDARAELDKVAEYFEAVAADRNVRLSVAGDASIHADQTLLR
HHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RAVTNLLDNALRHAPAGSTVRVDIARRAGEVGEAGDGTCIRVVNAGPAIAPEVLPHIFGR
HHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
FYRADPSRRNSAASTGLGLAIVDSIMRLHGGRALVSSSDGETVFSLLFPAGGDPRPHAVA
HHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCEEHHHEECCCCCCCCCCCC
>Mature Secondary Structure
MRLPSSLTARLTLAYGLATAAILAGVAAYLSSALSAQLQGRDESELVSEVLLVRHLLREV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RNEDEVRADTHRFADIAMGHEGLILVVRTPQGDPLVTLNPRNEAIPPLHVLPVTTQPHKS
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCHHH
MLEAWRPKSGAQSPAIAALGQLGDTGRSVEIVVLRDAGYRVALMREYRHRLLAATLCGAA
HHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VAAALGFVLARRGLRPLRRMAADAAAVTTSRLATRLDAGSAPAELRELARELNGMLARLQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DSFSRLSAFSADLAHDFRTPISNLVGQTEVTLAQSRSVAEYEALLESNLEEYERLSRMIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
NMLFLARADHAQVAMGVRGLDARAELDKVAEYFEAVAADRNVRLSVAGDASIHADQTLLR
HHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RAVTNLLDNALRHAPAGSTVRVDIARRAGEVGEAGDGTCIRVVNAGPAIAPEVLPHIFGR
HHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
FYRADPSRRNSAASTGLGLAIVDSIMRLHGGRALVSSSDGETVFSLLFPAGGDPRPHAVA
HHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCEEHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9044283 [H]