| Definition | Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_007348 |
| Length | 2,726,152 |
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The map label for this gene is ykvO [H]
Identifier: 73537526
GI number: 73537526
Start: 276855
End: 277619
Strand: Reverse
Name: ykvO [H]
Synonym: Reut_B3691
Alternate gene names: 73537526
Gene position: 277619-276855 (Counterclockwise)
Preceding gene: 73537528
Following gene: 73537523
Centisome position: 10.18
GC content: 64.05
Gene sequence:
>765_bases ATGGCACGCAAGCTCGACAACAAAGTTGCACTGGTTACCGGCGGAACCAGCGGCATCGGCCTCGCTACTGCGCAGCGCTT CGCCACGGAAGGCGCGCACGTCTATCTCACCGGCCGTCGTCAGGCAGAACTTGATACCGCGGTGAAGAGCATCCGCGAAG CAGGCGGCAACGCGACGGGGGTACGTGTCGACTCAACAAAGCTGGGTGAACTTGACGCGCTGTATGCGCAGATCAAGGAA GAACAGGGGCATCTCGACGTGCTGTTCGCGAACGCCGGCGGCGGTTCGATGCTGCCGCTCGGCAGCATCACCGAAGAGCA CTACGACGACACCTTCGATCGCAATGTGAAGGGCGTGCTCTTCACGGTGCAAAAAGCGCTGCCGCTGCTCGCCCAGGGCG CGTCTGTGATTCTAACTGGTTCAACGGCAGGCAGCGCGGGTACGGCTGCATTCAGCGTGTATTCGGCCTCGAAAGCCGCG GTGCGCGCCTTCGTACGCAGTTGGATTCTCGACCTGAAGGATCGCCGCATCCGCGTGAACACAATCAGCCCGGGCGCGAC GCGCACGCCTGGCCTGCTCGACCTCGCCGGTGACGATATCACTCAACGCCAGGGCCTGGCCGACTACCTGGCTGCCCAGA TCCCGATGGGGCGCCTCGGCGAGCCCGGCGAGATCGCGAGCGCCGCGCTGTTTCTTGCCTCGGAGGACGCGAGCTTCGTG AACGGCGTCGAGTTGTTCGTCGACGGCGGCCAGCAGCAGGTCTAA
Upstream 100 bases:
>100_bases AATCAATTCTATTTCGAAAAGCCGAGGGCGTCGATATTCTCCTCACATCGCAGCACGCAACCACGCAGTCATTCACTTAC CGAACGAAAGGAGAAATGCC
Downstream 100 bases:
>100_bases GCGGAGCTCTCGCGCGCGGGTGCGATGGTTCGCCCTGGGCAGCGAGCCGAGCAGCTTCGTCACCGCACGCTGGCCGGACG TGCCGGGACGGCGGCAACGG
Product: Short-chain dehydrogenase/reductase SDR
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 254; Mature: 253
Protein sequence:
>254_residues MARKLDNKVALVTGGTSGIGLATAQRFATEGAHVYLTGRRQAELDTAVKSIREAGGNATGVRVDSTKLGELDALYAQIKE EQGHLDVLFANAGGGSMLPLGSITEEHYDDTFDRNVKGVLFTVQKALPLLAQGASVILTGSTAGSAGTAAFSVYSASKAA VRAFVRSWILDLKDRRIRVNTISPGATRTPGLLDLAGDDITQRQGLADYLAAQIPMGRLGEPGEIASAALFLASEDASFV NGVELFVDGGQQQV
Sequences:
>Translated_254_residues MARKLDNKVALVTGGTSGIGLATAQRFATEGAHVYLTGRRQAELDTAVKSIREAGGNATGVRVDSTKLGELDALYAQIKE EQGHLDVLFANAGGGSMLPLGSITEEHYDDTFDRNVKGVLFTVQKALPLLAQGASVILTGSTAGSAGTAAFSVYSASKAA VRAFVRSWILDLKDRRIRVNTISPGATRTPGLLDLAGDDITQRQGLADYLAAQIPMGRLGEPGEIASAALFLASEDASFV NGVELFVDGGQQQV >Mature_253_residues ARKLDNKVALVTGGTSGIGLATAQRFATEGAHVYLTGRRQAELDTAVKSIREAGGNATGVRVDSTKLGELDALYAQIKEE QGHLDVLFANAGGGSMLPLGSITEEHYDDTFDRNVKGVLFTVQKALPLLAQGASVILTGSTAGSAGTAAFSVYSASKAAV RAFVRSWILDLKDRRIRVNTISPGATRTPGLLDLAGDDITQRQGLADYLAAQIPMGRLGEPGEIASAALFLASEDASFVN GVELFVDGGQQQV
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=34.1176470588235, Blast_Score=135, Evalue=4e-32, Organism=Homo sapiens, GI5031737, Length=248, Percent_Identity=33.0645161290323, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI126723750, Length=258, Percent_Identity=32.5581395348837, Blast_Score=115, Evalue=5e-26, Organism=Homo sapiens, GI33667109, Length=188, Percent_Identity=34.5744680851064, Blast_Score=102, Evalue=3e-22, Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=34.7593582887701, Blast_Score=101, Evalue=8e-22, Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=29.6, Blast_Score=97, Evalue=1e-20, Organism=Homo sapiens, GI10190704, Length=255, Percent_Identity=31.3725490196078, Blast_Score=95, Evalue=5e-20, Organism=Homo sapiens, GI15277342, Length=267, Percent_Identity=30.7116104868914, Blast_Score=92, Evalue=3e-19, Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=32, Blast_Score=92, Evalue=4e-19, Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=31.8965517241379, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI7705925, Length=243, Percent_Identity=28.3950617283951, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI19923817, Length=257, Percent_Identity=25.6809338521401, Blast_Score=78, Evalue=6e-15, Organism=Homo sapiens, GI209862901, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI19923983, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=28.5171102661597, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI4503301, Length=253, Percent_Identity=27.6679841897233, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI223718074, Length=189, Percent_Identity=31.2169312169312, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI20149619, Length=239, Percent_Identity=28.4518828451883, Blast_Score=64, Evalue=9e-11, Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=36.1111111111111, Blast_Score=112, Evalue=2e-26, Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=33.201581027668, Blast_Score=112, Evalue=2e-26, Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=32.5490196078431, Blast_Score=111, Evalue=5e-26, Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=30.241935483871, Blast_Score=109, Evalue=2e-25, Organism=Escherichia coli, GI87082100, Length=251, Percent_Identity=30.2788844621514, Blast_Score=107, Evalue=9e-25, Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=30.5555555555556, Blast_Score=92, Evalue=4e-20, Organism=Escherichia coli, GI2367365, Length=244, Percent_Identity=30.327868852459, Blast_Score=87, Evalue=9e-19, Organism=Escherichia coli, GI1786812, Length=248, Percent_Identity=31.8548387096774, Blast_Score=87, Evalue=1e-18, Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI1788459, Length=248, Percent_Identity=29.8387096774194, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI2367175, Length=251, Percent_Identity=29.4820717131474, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1787526, Length=191, Percent_Identity=30.8900523560209, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1786701, Length=189, Percent_Identity=30.1587301587302, Blast_Score=67, Evalue=8e-13, Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=32.8301886792453, Blast_Score=117, Evalue=6e-27, Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=36.5019011406844, Blast_Score=116, Evalue=1e-26, Organism=Caenorhabditis elegans, GI17559104, Length=259, Percent_Identity=33.5907335907336, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17560220, Length=267, Percent_Identity=32.9588014981273, Blast_Score=110, Evalue=6e-25, Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=31.5175097276265, Blast_Score=110, Evalue=9e-25, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=33.7078651685393, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=32.7137546468402, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17562904, Length=260, Percent_Identity=31.5384615384615, Blast_Score=107, Evalue=8e-24, Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=33.0798479087452, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17560150, Length=263, Percent_Identity=31.9391634980989, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17562906, Length=260, Percent_Identity=32.6923076923077, Blast_Score=103, Evalue=9e-23, Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=32, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17565030, Length=268, Percent_Identity=33.2089552238806, Blast_Score=102, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17536025, Length=257, Percent_Identity=34.2412451361868, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=32.319391634981, Blast_Score=99, Evalue=1e-21, Organism=Caenorhabditis elegans, GI72000259, Length=259, Percent_Identity=32.8185328185328, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=28.0155642023346, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17544670, Length=269, Percent_Identity=29.7397769516729, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=30.6772908366534, Blast_Score=97, Evalue=9e-21, Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=30.1204819277108, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17531453, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17563726, Length=256, Percent_Identity=29.6875, Blast_Score=91, Evalue=7e-19, Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=30.4182509505703, Blast_Score=90, Evalue=9e-19, Organism=Caenorhabditis elegans, GI25147288, Length=264, Percent_Identity=30.6818181818182, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17561272, Length=258, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI17536651, Length=251, Percent_Identity=29.8804780876494, Blast_Score=87, Evalue=9e-18, Organism=Caenorhabditis elegans, GI115534694, Length=256, Percent_Identity=30.078125, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17538182, Length=263, Percent_Identity=32.319391634981, Blast_Score=81, Evalue=4e-16, Organism=Caenorhabditis elegans, GI17508895, Length=214, Percent_Identity=25.7009345794392, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17508651, Length=255, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=7e-13, Organism=Caenorhabditis elegans, GI71997099, Length=266, Percent_Identity=26.6917293233083, Blast_Score=68, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17567345, Length=198, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=6e-12, Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=26.8774703557312, Blast_Score=82, Evalue=6e-17, Organism=Saccharomyces cerevisiae, GI6323882, Length=197, Percent_Identity=33.502538071066, Blast_Score=80, Evalue=3e-16, Organism=Saccharomyces cerevisiae, GI6322067, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=3e-14, Organism=Saccharomyces cerevisiae, GI6322227, Length=195, Percent_Identity=30.2564102564103, Blast_Score=68, Evalue=2e-12, Organism=Drosophila melanogaster, GI21355319, Length=255, Percent_Identity=32.9411764705882, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI23397609, Length=259, Percent_Identity=35.9073359073359, Blast_Score=123, Evalue=9e-29, Organism=Drosophila melanogaster, GI24643142, Length=254, Percent_Identity=32.2834645669291, Blast_Score=112, Evalue=2e-25, Organism=Drosophila melanogaster, GI24644339, Length=247, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=2e-24, Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=36.3636363636364, Blast_Score=105, Evalue=3e-23, Organism=Drosophila melanogaster, GI28571526, Length=261, Percent_Identity=31.4176245210728, Blast_Score=105, Evalue=3e-23, Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=32.0158102766798, Blast_Score=103, Evalue=8e-23, Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=30.5882352941176, Blast_Score=93, Evalue=2e-19, Organism=Drosophila melanogaster, GI17737361, Length=260, Percent_Identity=31.1538461538462, Blast_Score=83, Evalue=2e-16, Organism=Drosophila melanogaster, GI24640492, Length=234, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI281364494, Length=244, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI116007236, Length=247, Percent_Identity=25.9109311740891, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI24581726, Length=196, Percent_Identity=29.0816326530612, Blast_Score=64, Evalue=7e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26551; Mature: 26420
Theoretical pI: Translated: 5.35; Mature: 5.35
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 0.8 %Met (Mature Protein) 0.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MARKLDNKVALVTGGTSGIGLATAQRFATEGAHVYLTGRRQAELDTAVKSIREAGGNATG CCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCE VRVDSTKLGELDALYAQIKEEQGHLDVLFANAGGGSMLPLGSITEEHYDDTFDRNVKGVL EEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHE FTVQKALPLLAQGASVILTGSTAGSAGTAAFSVYSASKAAVRAFVRSWILDLKDRRIRVN EEHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE TISPGATRTPGLLDLAGDDITQRQGLADYLAAQIPMGRLGEPGEIASAALFLASEDASFV EECCCCCCCCCEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHEEEEEECCCCCHH NGVELFVDGGQQQV CCEEEEEECCCCCC >Mature Secondary Structure ARKLDNKVALVTGGTSGIGLATAQRFATEGAHVYLTGRRQAELDTAVKSIREAGGNATG CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCE VRVDSTKLGELDALYAQIKEEQGHLDVLFANAGGGSMLPLGSITEEHYDDTFDRNVKGVL EEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHE FTVQKALPLLAQGASVILTGSTAGSAGTAAFSVYSASKAAVRAFVRSWILDLKDRRIRVN EEHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE TISPGATRTPGLLDLAGDDITQRQGLADYLAAQIPMGRLGEPGEIASAALFLASEDASFV EECCCCCCCCCEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHEEEEEECCCCCHH NGVELFVDGGQQQV CCEEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]