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The map label for this gene is cheB1

Identifier: 73537483

GI number: 73537483

Start: 223922

End: 224947

Strand: Reverse

Name: cheB1

Synonym: Reut_B3648

Alternate gene names: 73537483

Gene position: 224947-223922 (Counterclockwise)

Preceding gene: 73537484

Following gene: 73537482

Centisome position: 8.25

GC content: 69.88

Gene sequence:

>1026_bases
ATGAAAGTCGGCATCGTCAACGATTCGGCACTGGCTGTCGCCGCGCTGCGCCGCGCGATTGCGCTGGACCCGGCGTTCGA
GATCGTCTGGGTCGCCGGCGATGGCGAGCAGGCGGTACAGCTCGCCGCCTCCCATACGCCCGACGTGATCCTGATGGACC
TGCTGATGCCGGTGATGGACGGCGTCGAAGCCACGCGCCGCATCATGGCGGCCACGCCGTGCCCCATCGTCGTGGTCACG
ACCGACCTCGGCCGCAACGCCACGCAGGTGTTCGACGCCATGGGCCACGGCGCGATCGACGCCGTGGACACCCCCACGCT
GACCGAGTCCGACGCCAAGCTGACCGCGGGGCCGCTGCTGCGCAAGATGCGCAATATCGGGCGCTTGGTCGCCGGCCGTG
CGGCACCGCGCCCTGCCATCACGGAAATGCCGGGCACATTGGCGGCATCGCGCCTGGTAGCCATCGGCGCCTCGGCAGGC
GGGCCGGCGGCGCTGGCCAAGCTGCTTGGCGCCCTGCCCGCCGACTTTTGCGCGGCGGTGGTCGCCGTCCAGCACGTCGA
TGAAGCGTTCGCTCCCGGCATGGCCGACTGGCTCGACAGCCAGTGCGCGCTGCCGGTACGCGTGGCGGCGCCGGGCGAAA
CTCCACAGGCCGGCACGGTACTCATCGCGGGCACCAACAATCATCTGCGGCTGCTAGGGTCAAACCGCATGGCCTATACC
GAACAGCCCAGCGAATATCTGTATCGGCCGTCCATCGACGTGTTCTTCGAAAGCGTGGTGGAGTACTGGCGCGGACAGGC
CATCGGCGTACTGCTGACCGGCATGGGCCGCGACGGCGCCAGCGGCCTCAAGGCCATGCGCGACCATGGTTGCCTGACCA
TCGCGCAGGACCAGGCCACCAGCGCCGTCTATGGCATGCCCAAGGCAGCCGCAGCGATGGGCGCCGCCAGCGAGATCCTG
CCGCTCACGGCCATTGCGCCGCGCCTGGTGCAGGCCTGCTGCAGCGTGCGCCCGCCGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGGCCTGGAAGCCGGCGCGGATTACTACCTGGCCAAAGGCAGTTTCCACGACGCTGCGCTGCTCGACGCAGTGCACGAC
CTGATCGGCGAGGCAAGCAC

Downstream 100 bases:

>100_bases
CAGGCGCAGCAAGAAAACCGATATCAACGAGACGACACAGATCGCCGATGCTCAACCAGGACGTTTGGGGCCGAGCCATC
AAGGAAGCCAGCATGCAACA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVGIVNDSALAVAALRRAIALDPAFEIVWVAGDGEQAVQLAASHTPDVILMDLLMPVMDGVEATRRIMAATPCPIVVVT
TDLGRNATQVFDAMGHGAIDAVDTPTLTESDAKLTAGPLLRKMRNIGRLVAGRAAPRPAITEMPGTLAASRLVAIGASAG
GPAALAKLLGALPADFCAAVVAVQHVDEAFAPGMADWLDSQCALPVRVAAPGETPQAGTVLIAGTNNHLRLLGSNRMAYT
EQPSEYLYRPSIDVFFESVVEYWRGQAIGVLLTGMGRDGASGLKAMRDHGCLTIAQDQATSAVYGMPKAAAAMGAASEIL
PLTAIAPRLVQACCSVRPPPG

Sequences:

>Translated_341_residues
MKVGIVNDSALAVAALRRAIALDPAFEIVWVAGDGEQAVQLAASHTPDVILMDLLMPVMDGVEATRRIMAATPCPIVVVT
TDLGRNATQVFDAMGHGAIDAVDTPTLTESDAKLTAGPLLRKMRNIGRLVAGRAAPRPAITEMPGTLAASRLVAIGASAG
GPAALAKLLGALPADFCAAVVAVQHVDEAFAPGMADWLDSQCALPVRVAAPGETPQAGTVLIAGTNNHLRLLGSNRMAYT
EQPSEYLYRPSIDVFFESVVEYWRGQAIGVLLTGMGRDGASGLKAMRDHGCLTIAQDQATSAVYGMPKAAAAMGAASEIL
PLTAIAPRLVQACCSVRPPPG
>Mature_341_residues
MKVGIVNDSALAVAALRRAIALDPAFEIVWVAGDGEQAVQLAASHTPDVILMDLLMPVMDGVEATRRIMAATPCPIVVVT
TDLGRNATQVFDAMGHGAIDAVDTPTLTESDAKLTAGPLLRKMRNIGRLVAGRAAPRPAITEMPGTLAASRLVAIGASAG
GPAALAKLLGALPADFCAAVVAVQHVDEAFAPGMADWLDSQCALPVRVAAPGETPQAGTVLIAGTNNHLRLLGSNRMAYT
EQPSEYLYRPSIDVFFESVVEYWRGQAIGVLLTGMGRDGASGLKAMRDHGCLTIAQDQATSAVYGMPKAAAAMGAASEIL
PLTAIAPRLVQACCSVRPPPG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=336, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_CUPPJ (Q46V28)

Other databases:

- EMBL:   CP000091
- RefSeq:   YP_297850.1
- ProteinModelPortal:   Q46V28
- SMR:   Q46V28
- GeneID:   3613877
- GenomeReviews:   CP000091_GR
- KEGG:   reu:Reut_B3648
- NMPDR:   fig|264198.3.peg.4546
- HOGENOM:   HBG705324
- OMA:   FFESVVE
- ProtClustDB:   PRK12555
- BioCyc:   REUT264198:REUT_B3648-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 35278; Mature: 35278

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 158-158 ACT_SITE 185-185 ACT_SITE 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIVNDSALAVAALRRAIALDPAFEIVWVAGDGEQAVQLAASHTPDVILMDLLMPVMD
CEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHC
GVEATRRIMAATPCPIVVVTTDLGRNATQVFDAMGHGAIDAVDTPTLTESDAKLTAGPLL
CHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
RKMRNIGRLVAGRAAPRPAITEMPGTLAASRLVAIGASAGGPAALAKLLGALPADFCAAV
HHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHEEECCCCCHHHHHHHHHCCCHHHHHHH
VAVQHVDEAFAPGMADWLDSQCALPVRVAAPGETPQAGTVLIAGTNNHLRLLGSNRMAYT
HHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCEEEC
EQPSEYLYRPSIDVFFESVVEYWRGQAIGVLLTGMGRDGASGLKAMRDHGCLTIAQDQAT
CCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCC
SAVYGMPKAAAAMGAASEILPLTAIAPRLVQACCSVRPPPG
HHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVGIVNDSALAVAALRRAIALDPAFEIVWVAGDGEQAVQLAASHTPDVILMDLLMPVMD
CEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHC
GVEATRRIMAATPCPIVVVTTDLGRNATQVFDAMGHGAIDAVDTPTLTESDAKLTAGPLL
CHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
RKMRNIGRLVAGRAAPRPAITEMPGTLAASRLVAIGASAGGPAALAKLLGALPADFCAAV
HHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHEEECCCCCHHHHHHHHHCCCHHHHHHH
VAVQHVDEAFAPGMADWLDSQCALPVRVAAPGETPQAGTVLIAGTNNHLRLLGSNRMAYT
HHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCEEEC
EQPSEYLYRPSIDVFFESVVEYWRGQAIGVLLTGMGRDGASGLKAMRDHGCLTIAQDQAT
CCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCC
SAVYGMPKAAAAMGAASEILPLTAIAPRLVQACCSVRPPPG
HHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA