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The map label for this gene is fixJ [H]

Identifier: 73537437

GI number: 73537437

Start: 161411

End: 162031

Strand: Reverse

Name: fixJ [H]

Synonym: Reut_B3602

Alternate gene names: 73537437

Gene position: 162031-161411 (Counterclockwise)

Preceding gene: 73537438

Following gene: 73537436

Centisome position: 5.94

GC content: 64.25

Gene sequence:

>621_bases
GTGTCGGGTAACCCATCCATCGTCTACGTTCTCGATGACGACGAGATCGTCTTGTGGCTCTATCGGGAACTGCTTCAGCA
CCACGGCGTGGTCCTGAGACCGTTCACATCGCCGCGGGCGTTTCTCGACAGCTACGCGCCCAGTCCGTGCGAATGCCTGG
TCTGCGATCTGCGCATGCCGGAACTCTGCGGACTTGGCGTGCAGAACGAACTGCTTTCCACCGGCGATTGCCTGCCCATC
ATCTTCGTGTCCGGTCACGCGGAAGTCTCGTCGGTGGTGGAAGCCATGCGTGTCGGCGCGTTCGACTTCCTGGAAAAACC
CGTGGACGGCGAACGCCTGGTGCAGACCGTCCGGGCCGCACTGGAACACAGCCGCGGCCTGCATGCCCGGCGGCTGGCCA
TGAGCGAGCGCCGCAAGCGGCTGGCCACGCTCACGCCGAAAGAGCGCGAGATTGCCGAACACGTCGCCAGCGGCAAGTCC
AGCCGCCAGATCTCCGAAGCGCTGAACATCAGCGTGCGCACGGTGGAGAACCATCGCGCCCGCGTGACCGAAAAGCTCTG
CATCACGTCCGTGGCCGAACTCGTGCGGCTGCTGTACGACCTGCCTGATCCTGGCGTCTGA

Upstream 100 bases:

>100_bases
CGCCATTTGAACCTGCCTGCTGCCTTGTGCCCGCCCCGTGCAGGGCCTGGCAGTCCGGACCCGACCAGCGGTATGGCATC
AGCACAAGGAGTAAGTACCC

Downstream 100 bases:

>100_bases
GACGCCCCTTCGGTTTCCGCGCATTTGCGCGGCATGGCCGTGCAGGACAGCATCCAAACGCGCTACACAGCACTCTCCGC
CGCCATGACGGCGGCATTGG

Product: LuxR response regulator receiver

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSGNPSIVYVLDDDEIVLWLYRELLQHHGVVLRPFTSPRAFLDSYAPSPCECLVCDLRMPELCGLGVQNELLSTGDCLPI
IFVSGHAEVSSVVEAMRVGAFDFLEKPVDGERLVQTVRAALEHSRGLHARRLAMSERRKRLATLTPKEREIAEHVASGKS
SRQISEALNISVRTVENHRARVTEKLCITSVAELVRLLYDLPDPGV

Sequences:

>Translated_206_residues
MSGNPSIVYVLDDDEIVLWLYRELLQHHGVVLRPFTSPRAFLDSYAPSPCECLVCDLRMPELCGLGVQNELLSTGDCLPI
IFVSGHAEVSSVVEAMRVGAFDFLEKPVDGERLVQTVRAALEHSRGLHARRLAMSERRKRLATLTPKEREIAEHVASGKS
SRQISEALNISVRTVENHRARVTEKLCITSVAELVRLLYDLPDPGV
>Mature_205_residues
SGNPSIVYVLDDDEIVLWLYRELLQHHGVVLRPFTSPRAFLDSYAPSPCECLVCDLRMPELCGLGVQNELLSTGDCLPII
FVSGHAEVSSVVEAMRVGAFDFLEKPVDGERLVQTVRAALEHSRGLHARRLAMSERRKRLATLTPKEREIAEHVASGKSS
RQISEALNISVRTVENHRARVTEKLCITSVAELVRLLYDLPDPGV

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=31.3559322033898, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=178, Percent_Identity=25.2808988764045, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=30.4347826086957, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22942; Mature: 22811

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNPSIVYVLDDDEIVLWLYRELLQHHGVVLRPFTSPRAFLDSYAPSPCECLVCDLRMP
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCHHEEEECCCCH
ELCGLGVQNELLSTGDCLPIIFVSGHAEVSSVVEAMRVGAFDFLEKPVDGERLVQTVRAA
HHHCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHH
LEHSRGLHARRLAMSERRKRLATLTPKEREIAEHVASGKSSRQISEALNISVRTVENHRA
HHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHHHHH
RVTEKLCITSVAELVRLLYDLPDPGV
HHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGNPSIVYVLDDDEIVLWLYRELLQHHGVVLRPFTSPRAFLDSYAPSPCECLVCDLRMP
CCCCCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCHHEEEECCCCH
ELCGLGVQNELLSTGDCLPIIFVSGHAEVSSVVEAMRVGAFDFLEKPVDGERLVQTVRAA
HHHCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHH
LEHSRGLHARRLAMSERRKRLATLTPKEREIAEHVASGKSSRQISEALNISVRTVENHRA
HHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHHHHH
RVTEKLCITSVAELVRLLYDLPDPGV
HHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]