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The map label for this gene is yagI [H]

Identifier: 73537435

GI number: 73537435

Start: 159055

End: 159891

Strand: Reverse

Name: yagI [H]

Synonym: Reut_B3600

Alternate gene names: 73537435

Gene position: 159891-159055 (Counterclockwise)

Preceding gene: 73537436

Following gene: 73537427

Centisome position: 5.87

GC content: 67.38

Gene sequence:

>837_bases
ATGCCGAAGCAGAGCCCCGACATCGACAACAACGCCGACACCGACGACGCCAAAGACCAGCAACTGGTCTCGCCCGTCAT
CCGCGGCCTGCGGCTGCTGCGCTTCATTGCCGAAGGCGGCTCGACCGCCAATCTCAGCGAAGTCGGCCGGCGCATCGACG
TCAACCGCGTTACGGTCATGCGGCTGCTCGCCACGCTCGAACACGAAGGCATGCTGGAACGCCTGCCGCAGGGCGGGCAT
GAGATTGGATTCGGGTTCCTGAAGCTGGCGTCTCGCGTGCTGGCGGCCAATGATCTGACCACGTTCGCGCGGCGTGTGCT
TGCGCGGCTCAGCGGATCGCTGCAGCTCTCGGCCTACCTCGTCGTGCGTGATGGCGGCCATGCGCTGTACCTGCTGCGCG
ATATGCCGAACACATCGCTGGTCAGCAATATCCAGGTCGGCAGCCGCGTCGCCGCGCACCTGACTACGCCGGGGCGCATG
CTGATTGCCGATCTACCGCCTGAAGAATTGCGCGCCTTGCTTGGTGATGACCCGCTGCCAACCGTGACCGGCCAGAGCCC
CGCCACGCATGCGCGGCTGGCCGACATCCTCGCAGCCGACCGCGAACGCGGCTGCGCATGGAGTTTTTCCGCGTTTGAAG
CCGGTATCGATTCTTGCGCCGCGCCCGTGCGCGACGCCACCGGCGAGGTGATCGCCGCCATCAGCGTGGCCGGCCCGCAG
CAGCGCTTCGAAACCGACAACACGCTGCGCGAACGCGTCGAGCGCGAGGTCAAGGCCGCCGCGCGCGACCTGTCCGCGTT
GCTCGGTGACCGCGCTAGCAGTGGCCGGCAGCGCTAG

Upstream 100 bases:

>100_bases
TGTACGATTACGACGCCTGGAACGTTGCGCTGGGTTGATCCCCACGCAACCCAACCTGTACGGCCGCACTTCGCGGCCGT
TTGCCTTTTTCTCCTTGACC

Downstream 100 bases:

>100_bases
CGCAGTCCGGCCGTTATTCGGCCTTGATGTCGTATTCGCGGATCAGCTTTGCCCACGCGGCCGTTTCTTCACGGATGCGC
GCGGCGAGCGCATCAGGCGT

Product: regulatory protein IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPKQSPDIDNNADTDDAKDQQLVSPVIRGLRLLRFIAEGGSTANLSEVGRRIDVNRVTVMRLLATLEHEGMLERLPQGGH
EIGFGFLKLASRVLAANDLTTFARRVLARLSGSLQLSAYLVVRDGGHALYLLRDMPNTSLVSNIQVGSRVAAHLTTPGRM
LIADLPPEELRALLGDDPLPTVTGQSPATHARLADILAADRERGCAWSFSAFEAGIDSCAAPVRDATGEVIAAISVAGPQ
QRFETDNTLRERVEREVKAAARDLSALLGDRASSGRQR

Sequences:

>Translated_278_residues
MPKQSPDIDNNADTDDAKDQQLVSPVIRGLRLLRFIAEGGSTANLSEVGRRIDVNRVTVMRLLATLEHEGMLERLPQGGH
EIGFGFLKLASRVLAANDLTTFARRVLARLSGSLQLSAYLVVRDGGHALYLLRDMPNTSLVSNIQVGSRVAAHLTTPGRM
LIADLPPEELRALLGDDPLPTVTGQSPATHARLADILAADRERGCAWSFSAFEAGIDSCAAPVRDATGEVIAAISVAGPQ
QRFETDNTLRERVEREVKAAARDLSALLGDRASSGRQR
>Mature_277_residues
PKQSPDIDNNADTDDAKDQQLVSPVIRGLRLLRFIAEGGSTANLSEVGRRIDVNRVTVMRLLATLEHEGMLERLPQGGHE
IGFGFLKLASRVLAANDLTTFARRVLARLSGSLQLSAYLVVRDGGHALYLLRDMPNTSLVSNIQVGSRVAAHLTTPGRML
IADLPPEELRALLGDDPLPTVTGQSPATHARLADILAADRERGCAWSFSAFEAGIDSCAAPVRDATGEVIAAISVAGPQQ
RFETDNTLRERVEREVKAAARDLSALLGDRASSGRQR

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=26.0162601626016, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082359, Length=241, Percent_Identity=26.9709543568465, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788581, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788131, Length=262, Percent_Identity=26.7175572519084, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29962; Mature: 29831

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQSPDIDNNADTDDAKDQQLVSPVIRGLRLLRFIAEGGSTANLSEVGRRIDVNRVTVM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
RLLATLEHEGMLERLPQGGHEIGFGFLKLASRVLAANDLTTFARRVLARLSGSLQLSAYL
HHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
VVRDGGHALYLLRDMPNTSLVSNIQVGSRVAAHLTTPGRMLIADLPPEELRALLGDDPLP
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHCCCCCC
TVTGQSPATHARLADILAADRERGCAWSFSAFEAGIDSCAAPVRDATGEVIAAISVAGPQ
CCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
QRFETDNTLRERVEREVKAAARDLSALLGDRASSGRQR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PKQSPDIDNNADTDDAKDQQLVSPVIRGLRLLRFIAEGGSTANLSEVGRRIDVNRVTVM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
RLLATLEHEGMLERLPQGGHEIGFGFLKLASRVLAANDLTTFARRVLARLSGSLQLSAYL
HHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
VVRDGGHALYLLRDMPNTSLVSNIQVGSRVAAHLTTPGRMLIADLPPEELRALLGDDPLP
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHCCCCCC
TVTGQSPATHARLADILAADRERGCAWSFSAFEAGIDSCAAPVRDATGEVIAAISVAGPQ
CCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
QRFETDNTLRERVEREVKAAARDLSALLGDRASSGRQR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]