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The map label for this gene is fliY [H]

Identifier: 73537420

GI number: 73537420

Start: 142178

End: 142978

Strand: Reverse

Name: fliY [H]

Synonym: Reut_B3585

Alternate gene names: 73537420

Gene position: 142978-142178 (Counterclockwise)

Preceding gene: 73537421

Following gene: 73537419

Centisome position: 5.24

GC content: 60.42

Gene sequence:

>801_bases
ATGAAGCTGCTGCAATCGCTGTTGCAAAGCGCGCTGCTCGCGACCGTTCTCGGTTTCACCGCACCCGCCGTACATGCTGA
AGACCTGCTGGACACCGTCAAGGCCAGCGGCGTCCTGAAGGTCGGCATGGAGGGCACGTACCCCCCGTTCGGCTATCGAA
ATGGCCAGGGCCAGCTTGATGGCTTTGACGTCGCGGTGGCCAGGGCCGTGGCAGACAAGATGGGCCTCAAAGTTCAGTTC
ATTACCACCGAGTGGAGCGGCATCATCGCGGGCCTGCAGGCTGGCAAATTTGACGTCATCGTGAATCAGGTGAGCATGAC
GCCGGCGCGTAAGCAGGCACTCGATTTCACGCAGCCTTATGTCTATTCCGCGGTCCAGGTGCTTCAACGCAGCAACGACA
AGCGCGAATTCGGGTCGTTGGACGCGCTGAAGGGCAAATCCGTTGGCGTCACGCTCGGCAGCAACTATGCCGATCTTGCC
AAATCGGTGCCTGGCATCCAGGTGCAGACCTATCCGGGTACCTCGGAAAACCTGCGTGATCTCGCAGCCGGCCGTATCGA
TGCCGCGCTCAATGATCGGCTGATGCTGCCGTACCTGATCAAGAACGCCAACCTGCCACTACGTACCGGTGCGCTGCTGG
CCGGCACGAAGCTGGAAATGGGTATTCCCTACCGCAAGGGCAATCCCAAATTCGCCAAGGCCATTGACGACGCCCTGATT
GCGCTGCAACAGGACGGCACCTTGCGCAAGCTGTCCATCGAGTGGCTCGGCACCGATGCCAGCCAACCCCTCGCACAGTA
A

Upstream 100 bases:

>100_bases
GGCGAAGGTGCCATCAGCTATCAGGTAAACCGTTCCACGGGACCTGTCTGGCACTGATGTTGCCCTTCGACAATATCTAT
AACCACATTGGAGACAAGGC

Downstream 100 bases:

>100_bases
CCCCAACGCCTGACCGCCTGACCGCCTGACCGCCCGCCCGTCCGGTTGCGGCCGGGCGCGTCCGGTCAAAGCCATGGATC
GCATCATGAGCCCTTTTGAT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKLLQSLLQSALLATVLGFTAPAVHAEDLLDTVKASGVLKVGMEGTYPPFGYRNGQGQLDGFDVAVARAVADKMGLKVQF
ITTEWSGIIAGLQAGKFDVIVNQVSMTPARKQALDFTQPYVYSAVQVLQRSNDKREFGSLDALKGKSVGVTLGSNYADLA
KSVPGIQVQTYPGTSENLRDLAAGRIDAALNDRLMLPYLIKNANLPLRTGALLAGTKLEMGIPYRKGNPKFAKAIDDALI
ALQQDGTLRKLSIEWLGTDASQPLAQ

Sequences:

>Translated_266_residues
MKLLQSLLQSALLATVLGFTAPAVHAEDLLDTVKASGVLKVGMEGTYPPFGYRNGQGQLDGFDVAVARAVADKMGLKVQF
ITTEWSGIIAGLQAGKFDVIVNQVSMTPARKQALDFTQPYVYSAVQVLQRSNDKREFGSLDALKGKSVGVTLGSNYADLA
KSVPGIQVQTYPGTSENLRDLAAGRIDAALNDRLMLPYLIKNANLPLRTGALLAGTKLEMGIPYRKGNPKFAKAIDDALI
ALQQDGTLRKLSIEWLGTDASQPLAQ
>Mature_266_residues
MKLLQSLLQSALLATVLGFTAPAVHAEDLLDTVKASGVLKVGMEGTYPPFGYRNGQGQLDGFDVAVARAVADKMGLKVQF
ITTEWSGIIAGLQAGKFDVIVNQVSMTPARKQALDFTQPYVYSAVQVLQRSNDKREFGSLDALKGKSVGVTLGSNYADLA
KSVPGIQVQTYPGTSENLRDLAAGRIDAALNDRLMLPYLIKNANLPLRTGALLAGTKLEMGIPYRKGNPKFAKAIDDALI
ALQQDGTLRKLSIEWLGTDASQPLAQ

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=260, Percent_Identity=40, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787085, Length=259, Percent_Identity=27.4131274131274, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787031, Length=261, Percent_Identity=29.5019157088123, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788649, Length=231, Percent_Identity=29.4372294372294, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=28.755364806867, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787088, Length=260, Percent_Identity=27.3076923076923, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28493; Mature: 28493

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLQSLLQSALLATVLGFTAPAVHAEDLLDTVKASGVLKVGMEGTYPPFGYRNGQGQLD
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCC
GFDVAVARAVADKMGLKVQFITTEWSGIIAGLQAGKFDVIVNQVSMTPARKQALDFTQPY
HHHHHHHHHHHHHHCCEEEEEEECHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCHH
VYSAVQVLQRSNDKREFGSLDALKGKSVGVTLGSNYADLAKSVPGIQVQTYPGTSENLRD
HHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCEEEECCCCCHHHHH
LAAGRIDAALNDRLMLPYLIKNANLPLRTGALLAGTKLEMGIPYRKGNPKFAKAIDDALI
HHHHHHHHHHCCCEEEHHHHCCCCCCEECCCEEECCEEECCCCCCCCCCHHHHHHHHHHH
ALQQDGTLRKLSIEWLGTDASQPLAQ
EECCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKLLQSLLQSALLATVLGFTAPAVHAEDLLDTVKASGVLKVGMEGTYPPFGYRNGQGQLD
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCC
GFDVAVARAVADKMGLKVQFITTEWSGIIAGLQAGKFDVIVNQVSMTPARKQALDFTQPY
HHHHHHHHHHHHHHCCEEEEEEECHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCHH
VYSAVQVLQRSNDKREFGSLDALKGKSVGVTLGSNYADLAKSVPGIQVQTYPGTSENLRD
HHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCEEEECCCCCHHHHH
LAAGRIDAALNDRLMLPYLIKNANLPLRTGALLAGTKLEMGIPYRKGNPKFAKAIDDALI
HHHHHHHHHHCCCEEEHHHHCCCCCCEECCCEEECCEEECCCCCCCCCCHHHHHHHHHHH
ALQQDGTLRKLSIEWLGTDASQPLAQ
EECCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]