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The map label for this gene is cysL [H]

Identifier: 73537379

GI number: 73537379

Start: 93099

End: 94001

Strand: Reverse

Name: cysL [H]

Synonym: Reut_B3544

Alternate gene names: 73537379

Gene position: 94001-93099 (Counterclockwise)

Preceding gene: 73537382

Following gene: 73537377

Centisome position: 3.45

GC content: 64.45

Gene sequence:

>903_bases
ATGCATTACGACCTGACCGACCTGCGCCTTTTCCTCAACGTCGGTGAAACGGAAAACCTGACCCGCGCAGCGGAGCGCAG
TTTCCTGTCCCTGCCGGCAGCCAGCACGCGCATCAAGCAACTGGAAGAGGCGTTCCAGACCCAGCTCCTGATCCGCCAGG
TGAAAGGCGTCAGGCTGACCCCGGCCGGCGATACACTGCTGCACCATGCGCGCGAGGTGTTCCGCGAGCTCGAATGCCTG
CACTCCGACCTGCGTCCCTATGCCAAGGGCGTCAAGGGCAAGGTCCGCCTGCTGGCCAATACGACCGCCACCAACTCGTT
CCTGGCGACGGGCCTGTCCCGTTTTCTAAGTGAAAACCCTGACGTCGATGTGGAGCTGGAAGAGCATCTGTCGCAGGAAA
TCGTGTCCGCGATCAGCGCGGGCGCGGCGGACCTTGGCATTGTGGCGGGCGAAGTCGCGACCCAGGATCTCGACGTCATG
CACCTGTGCAGCGACGAACTGATCGTGATTGCGCCGGTCAACCACCCGCTGCCGCAGTTCAAGCGGCTGCACTTCGCCGA
CCTGCTGGATACCTGCCGCTTCGTCGGCCTGAACCAGTTCAGCGCGATCCAGTCCTTCCTCGACCGCATCGCCAGCGGCA
TGGGCAAGCGTATCAGCCTGCGCATCCAGGTCGGCAGCTTCGACGCGGTCTGCCGCATGGTCGAGGCCGGCGCCGGCATC
GCCATCGTGCCGAACAGCTGTGCGCGCCGCTATGCGAGTCGCAAGGTGCTGCGCTTTATCTCGCTGGAAGATGAATGGGC
GCGCCGCGAATTGCGCCTGTGCCGCCGCCCGGGCCGCGAACTGCCACAGTTCGCCGAAACCCTGATCCAGTACCTGGTCG
ACGCTGCGCGCGAGCCTGTATAG

Upstream 100 bases:

>100_bases
GCATGTCCGGATGTTATTGCCACGCGAGATACGATAAATTGGCGGCTGAAACATTGGAATTCACGAATCGCAAAAGTGGC
CTTTGGCCTGACGGAACCCC

Downstream 100 bases:

>100_bases
AGTTGGCAATACATGGCCGTTCGCCCACTGGCGGCGCTAGGCCACCAGCCTCGCGAGCGGCCGCAGCGACACCAGCATGA
CCGCGATCAGCAGCGCCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MHYDLTDLRLFLNVGETENLTRAAERSFLSLPAASTRIKQLEEAFQTQLLIRQVKGVRLTPAGDTLLHHAREVFRELECL
HSDLRPYAKGVKGKVRLLANTTATNSFLATGLSRFLSENPDVDVELEEHLSQEIVSAISAGAADLGIVAGEVATQDLDVM
HLCSDELIVIAPVNHPLPQFKRLHFADLLDTCRFVGLNQFSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFISLEDEWARRELRLCRRPGRELPQFAETLIQYLVDAAREPV

Sequences:

>Translated_300_residues
MHYDLTDLRLFLNVGETENLTRAAERSFLSLPAASTRIKQLEEAFQTQLLIRQVKGVRLTPAGDTLLHHAREVFRELECL
HSDLRPYAKGVKGKVRLLANTTATNSFLATGLSRFLSENPDVDVELEEHLSQEIVSAISAGAADLGIVAGEVATQDLDVM
HLCSDELIVIAPVNHPLPQFKRLHFADLLDTCRFVGLNQFSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFISLEDEWARRELRLCRRPGRELPQFAETLIQYLVDAAREPV
>Mature_300_residues
MHYDLTDLRLFLNVGETENLTRAAERSFLSLPAASTRIKQLEEAFQTQLLIRQVKGVRLTPAGDTLLHHAREVFRELECL
HSDLRPYAKGVKGKVRLLANTTATNSFLATGLSRFLSENPDVDVELEEHLSQEIVSAISAGAADLGIVAGEVATQDLDVM
HLCSDELIVIAPVNHPLPQFKRLHFADLLDTCRFVGLNQFSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
AIVPNSCARRYASRKVLRFISLEDEWARRELRLCRRPGRELPQFAETLIQYLVDAAREPV

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=24.1496598639456, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=269, Percent_Identity=25.6505576208178, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=304, Percent_Identity=25.3289473684211, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=26.4406779661017, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786713, Length=257, Percent_Identity=26.0700389105058, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.972027972028, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=124, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=286, Percent_Identity=26.5734265734266, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=24.3027888446215, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787806, Length=303, Percent_Identity=22.4422442244224, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=294, Percent_Identity=23.469387755102, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789506, Length=209, Percent_Identity=24.4019138755981, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=21.5827338129496, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789204, Length=283, Percent_Identity=25.0883392226148, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33462; Mature: 33462

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYDLTDLRLFLNVGETENLTRAAERSFLSLPAASTRIKQLEEAFQTQLLIRQVKGVRLT
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEC
PAGDTLLHHAREVFRELECLHSDLRPYAKGVKGKVRLLANTTATNSFLATGLSRFLSENP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCC
DVDVELEEHLSQEIVSAISAGAADLGIVAGEVATQDLDVMHLCSDELIVIAPVNHPLPQF
CCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHH
KRLHFADLLDTCRFVGLNQFSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCE
AIVPNSCARRYASRKVLRFISLEDEWARRELRLCRRPGRELPQFAETLIQYLVDAAREPV
EECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHYDLTDLRLFLNVGETENLTRAAERSFLSLPAASTRIKQLEEAFQTQLLIRQVKGVRLT
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEC
PAGDTLLHHAREVFRELECLHSDLRPYAKGVKGKVRLLANTTATNSFLATGLSRFLSENP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCC
DVDVELEEHLSQEIVSAISAGAADLGIVAGEVATQDLDVMHLCSDELIVIAPVNHPLPQF
CCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHH
KRLHFADLLDTCRFVGLNQFSAIQSFLDRIASGMGKRISLRIQVGSFDAVCRMVEAGAGI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCE
AIVPNSCARRYASRKVLRFISLEDEWARRELRLCRRPGRELPQFAETLIQYLVDAAREPV
EECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]