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The map label for this gene is exoA [H]

Identifier: 71910153

GI number: 71910153

Start: 342208

End: 343119

Strand: Reverse

Name: exoA [H]

Synonym: M5005_Spy_0339

Alternate gene names: 71910153

Gene position: 343119-342208 (Counterclockwise)

Preceding gene: 71910164

Following gene: 71910151

Centisome position: 18.66

GC content: 42.32

Gene sequence:

>912_bases
ATGGGGGCTAGGTGCCCAAAGTCGCTTATCGTTTCGTTTTTTGTTACAATAACAACAAGACTATCTGTTAGAAAGAGAAA
CCAAATGAAACTGATTTCATGGAACATTGATTCCTTAAATGCTGCTCTCACAGGAGAGTCCCCTCGTGCGCTTTTATCAC
GCGCTGTTTTAGACACTTTAGTCGCTCAAGATGCGGATATTATTGCCATCCAAGAAACTAAATTATCTGCTAAGGGGCCT
ACCAAAAAACATATCGAGACCCTACTTAGTTACTTTCCAAACTATTTGCATGTGTGGCGTTCTTCAGTTGAGCCTGCTAG
GAAAGGCTATGCTGGCACCATGTTCCTGTACAAAAACACGTTAAACCCTGTCATTACTTTTCCAGAAATTGGTGCCCCAA
CAACAATGGATGCTGAGGGCCGTATCATTACCCTAGAATTTGAAGACTTTTTCGTCACACAGGTTTACACGCCTAATGCT
GGTGATGGCCTTAGACGTTTAGATGACCGTCAAATTTGGGACCACAAATACGCTGATTATTTGACTGAACTGGATGCTCA
AAAGCCTGTTTTGGCTACGGGAGATTACAATGTTGCCCATAAAGAGATTGATTTGGCAAATCCAAACAGCAATCGACGTT
CTCCTGGTTTTACCGACGAAGAACGTCAAGGCTTTACTAACCTATTAGCAAGAGGCTTTACAGATACTTTTCGTCACGTG
CACGGCGATATTCCTCATGTTTATACCTGGTGGGCACAACGTAGCAAAACCAGCAAAATCAATAATACCGGTTGGAGAAT
TGACTATTGGCTAGCTTCAAACCGACTTGTGGATAAGGTTAAGCGTTCTGAAATGATTTCTTCTGGGGAACGCCAAGACC
ATACGCCAATTTTACTAGATATTGATCTCTAA

Upstream 100 bases:

>100_bases
TATTATAGTAATCCTACTCTTAACTGTCAAACGATATGCTCACTGATCACCTCCTTGTCTTGTTCTTACGCTGCTATGCT
TCATTTGGCTAGACTGTTTT

Downstream 100 bases:

>100_bases
TTTCTAAAAACAGATCCCAAAAAAGGACAAGGCGTTATAATGTTGCCTTGTCCTTTTTTGAGACTTTGTCGCATTATCCT
AAATCCAACTCTTGATATGG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGARCPKSLIVSFFVTITTRLSVRKRNQMKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGP
TKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNA
GDGLRRLDDRQIWDHKYADYLTELDAQKPVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHV
HGDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL

Sequences:

>Translated_303_residues
MGARCPKSLIVSFFVTITTRLSVRKRNQMKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGP
TKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNA
GDGLRRLDDRQIWDHKYADYLTELDAQKPVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHV
HGDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL
>Mature_302_residues
GARCPKSLIVSFFVTITTRLSVRKRNQMKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPT
KKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAG
DGLRRLDDRQIWDHKYADYLTELDAQKPVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHVH
GDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=276, Percent_Identity=36.9565217391304, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI18375503, Length=276, Percent_Identity=36.9565217391304, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI18375501, Length=276, Percent_Identity=36.9565217391304, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1788046, Length=287, Percent_Identity=27.5261324041812, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=37.0909090909091, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI221330655, Length=279, Percent_Identity=36.2007168458781, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17136678, Length=279, Percent_Identity=36.2007168458781, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 34518; Mature: 34386

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARCPKSLIVSFFVTITTRLSVRKRNQMKLISWNIDSLNAALTGESPRALLSRAVLDTL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHH
VAQDADIIAIQETKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNT
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECC
LNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADY
CCCEEECCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHH
LTELDAQKPVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHV
HHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HGDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLD
CCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
IDL
ECC
>Mature Secondary Structure 
GARCPKSLIVSFFVTITTRLSVRKRNQMKLISWNIDSLNAALTGESPRALLSRAVLDTL
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHH
VAQDADIIAIQETKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNT
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECC
LNPVITFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADY
CCCEEECCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHH
LTELDAQKPVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHV
HHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HGDIPHVYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLD
CCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
IDL
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]