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The map label for this gene is ybfL [H]

Identifier: 71910112

GI number: 71910112

Start: 309533

End: 310237

Strand: Reverse

Name: ybfL [H]

Synonym: M5005_Spy_0298

Alternate gene names: 71910112

Gene position: 310237-309533 (Counterclockwise)

Preceding gene: 71910135

Following gene: 71910111

Centisome position: 16.87

GC content: 38.3

Gene sequence:

>705_bases
ATGATTGATTTTATTATTTCTATTGATGATTGCGCAGTTGAATTGGATAGTCGTCAATCTTGGAAAATTCGCTACCCCTT
ATCAACCATTCTATTTCTTGTCTTCGTTTGTCAGTTAGCTGGCATTGAAACCTGGAAGGAGATGGAAGATTTTATTGAAA
TGAATGAACCATTGTTTGCGACCTACGTTGATTTGAGTGAAGGTTGTCCGTCTCATGATACCTTAGAGCGTGTGATTAGT
CTTGTTAATTCAGACCGTTTAAAAGAGCTTAAAGTTCAATTTGAGCAATCATTGACAAGCTTAGATGCCGTTCATCAACT
GATTTCAGTGGACGGTAAAACGATTCGAGGCAATCGAGGTAAAAATCAGAAGCCTGTTCATATTGTAACGGCTTATGATG
GGGGTCATCATCTTAGTTTGGGACAGGTAGCGGTTGAGGAGAAAAGTAATGAAATTGTTGCCATCCCTCAGCTATTGCGG
ACAATTGATATCCGTAAAAGCATTGTAACGATAGACGCAATGGGCACGCAGACGGCTATCGTTGATACGATTATAAAAGG
TAAAGCAGACTATTGCTTAGCCGTCAAAGGAAATCAAGAAACACTTTATGATGATATTGCTCTTTATTTTAGTGATGTCA
ACTTATTGGAAGAACTCCAAGAAAATGCGCAGTATTATCAGACTGTTGAAAAATCTAGAAGTTAG

Upstream 100 bases:

>100_bases
ACAATCACGCCAAACGCATGAAGAAACTTTTGTTTTCAGAAAACTAGATTCATAATGACAAAATCAATCAAAGTAGTACA
CTATAGATGAGGTGACTACG

Downstream 100 bases:

>100_bases
AGAATACTGGGTGTCTTCCGATATCAAATGGTTGTGTCAAAATTATCCCAAATGGCATAAGTTACGTGGTATTGGGATGA
CTCGTAACACGATTGATAAG

Product: transposase

Products: NA

Alternate protein names: H repeat-associated protein in rhsC-phrB intergenic region; ORF-H2 [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRS

Sequences:

>Translated_234_residues
MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRS
>Mature_234_residues
MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRS

Specific function: Unknown

COG id: COG5433

COG function: function code L; Transposase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787733, Length=220, Percent_Identity=38.1818181818182, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789896, Length=189, Percent_Identity=39.1534391534392, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786924, Length=189, Percent_Identity=38.0952380952381, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 26417; Mature: 26417

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFA
CEEEEEECCHHEEEECCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEE
TYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRG
EEEEHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAI
CCCCCEEEEEEECCCCEEEHHHHHECCCCCCEEEHHHHHHHHHHHHHEEEEECCCCHHHH
VDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRS
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDFIISIDDCAVELDSRQSWKIRYPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFA
CEEEEEECCHHEEEECCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEE
TYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLDAVHQLISVDGKTIRGNRG
EEEEHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAI
CCCCCEEEEEEECCCCEEEHHHHHECCCCCCEEEHHHHHHHHHHHHHEEEEECCCCHHHH
VDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRS
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8387990; 8905232; 9278503 [H]