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The map label for this gene is ulaA [H]

Identifier: 71909962

GI number: 71909962

Start: 160544

End: 161977

Strand: Direct

Name: ulaA [H]

Synonym: M5005_Spy_0148

Alternate gene names: 71909962

Gene position: 160544-161977 (Clockwise)

Preceding gene: 161936127

Following gene: 71909963

Centisome position: 8.73

GC content: 42.4

Gene sequence:

>1434_bases
ATGGAAATGCTCCTAGCTCCCCTAAACTGGTTCTCGCAAAATATTTTGCAAAACCCAGCGTTCTTTGTAGGTCTTTTGGT
TTTAATCGGGTATTTACTCCTTAAAAAACCCATTTATGAGGTCTTTGCTGGTTTTGTAAAGGCTACCGTTGGTTACTTGA
TTTTGAACGTTGGAGCAGGCGGTCTTGTAACGACTTTTCGTCCGATCTTGGTTGCTCTTGCCAAGAAATTTGAATTGAAA
GCCGCCGTTATTGATCCTTATTTTGGTTTGGCTGCTGCTAATACTAAGTTAGAAGAGATGGGCTTCATCAGTGTTGCTAC
GACAGCTCTCTTGATTGGATTTGGGGTCAACATTCTTTTGGTTGCACTTCGCAAGGTCACTAAAGTTCGTACCCTTTTCA
TCACAGGTCACATCATGGTCCAACAGGCAGCGACGATTTCGGTCTTTGTCCTTCTTTTAATCCCTCAATTCCAAAATGCT
TTTGGGGCATGGGCAGTCGGTATCATCTGTGGGCTTTACTGGGCTATCAGCTCAAACATGACCGTTGAAGCAACTCAACG
CCTTACAGGTGGCGGTGGGTTTGCGATTGGTCACCAACAACAATTTGCGATTTGGTTTGTGGATAAGGTTGCACCGTTCT
TTGGTAAAAAAGAAGAAAACCTTGATAACCTAAAATTACCAACCTTCTTAAACATTTTCCATGATACTGTTGTTGCGTCA
GCAACTTTGATGTTGGTCTTTTTCGGAGCTATCTTGGCTGTCCTTGGTCCAGACATTATGTCTGATGTGGACTTAATCGG
ACCTGGAGCATTCAACCCAGCTAAGCAAGCTTTCTTCATGTATATTTTGCAAACCTCTCTCACCTTCTCAGTTTATCTCT
TTATCTTGATGCAGGGTGTTCGCATGTTTGTTTCTGAATTGACCAATGCCTTTCAAGGGATCTCAAGCAAATTGCTTCCA
GGGTCATTCCCAGCGGTTGACGTTGCAGCTTCCTATGGCTTTGGTTCTTCAAATGCGGTGCTTTCAGGATTTGCCTTTGG
TTTGATTGGTCAATTAATCACCATTGCTCTTCTTGTTATCTTTAAAAATCCAATCTTAATTATCACAGGATTCGTTCCCG
TATTCTTTGATAATGCGGCAATTGCGGTTTATGCAGATAAACGTGGTGGCTGGAAAGCAGCGGTAGCTCTATCATTTATT
TCAGGTATTTTGCAAGTTGCACTTGGTGCTGTTGCTGTAGGTCTTTTAGGACTTACAGGTGGTTATCACGGTAATATCGA
CTTGGTTCTTCCATGGTTACCATTTGGGTACCTCTTCAAATTCCTTGGTATTGCAGGGTATGTGCTTGTTTGCATCTTCT
TACTTGCCATTCCACAATTGCAATTCGCAAAAGCTAAGGACAAAGAAGCTTATTACCGTGGGGAAGCTCAATAA

Upstream 100 bases:

>100_bases
CTTCTTTGTGTGATAGTTGGTCTTTTTTAAAACGCTTTCATTACCTATAATGAACATATAAACACTTTATCTTTATTAAC
TTTGAAAAGAGGTAGTTCTT

Downstream 100 bases:

>100_bases
CACTGCATCTTAAGAAATAGCTCTGCTATTTTAACAAAACAATAATAAAACATTTTCGGAGGAAAAAACAATGGTTAAAG
TCTTAACAGCATGTGGCAAT

Product: PTS system ascorbate-specific transporter subunit IIC

Products: protein histidine; sugar phosphate

Alternate protein names: Ascorbate-specific PTS system EIIC component [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAGFVKATVGYLILNVGAGGLVTTFRPILVALAKKFELK
AAVIDPYFGLAAANTKLEEMGFISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKEENLDNLKLPTFLNIFHDTVVAS
ATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQAFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLP
GSFPAVDVAASYGFGSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWKAAVALSFI
SGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLVCIFLLAIPQLQFAKAKDKEAYYRGEAQ

Sequences:

>Translated_477_residues
MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAGFVKATVGYLILNVGAGGLVTTFRPILVALAKKFELK
AAVIDPYFGLAAANTKLEEMGFISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKEENLDNLKLPTFLNIFHDTVVAS
ATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQAFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLP
GSFPAVDVAASYGFGSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWKAAVALSFI
SGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLVCIFLLAIPQLQFAKAKDKEAYYRGEAQ
>Mature_477_residues
MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAGFVKATVGYLILNVGAGGLVTTFRPILVALAKKFELK
AAVIDPYFGLAAANTKLEEMGFISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKEENLDNLKLPTFLNIFHDTVVAS
ATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQAFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLP
GSFPAVDVAASYGFGSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWKAAVALSFI
SGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLVCIFLLAIPQLQFAKAKDKEAYYRGEAQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active- transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3037

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaA family [H]

Homologues:

Organism=Escherichia coli, GI87082388, Length=461, Percent_Identity=39.4793926247288, Blast_Score=311, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: 2.7.1.69

Molecular weight: Translated: 51414; Mature: 51414

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAGFVKATVGYLILNVGAG
CCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGFISVATTALLIGFGVNILL
CHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNAFGAWAVGIICGLYWAISSNM
HHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKEENLDNLKLPTFLNIFHDTVVAS
EEEHHHHHCCCCCEEECCCCEEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQAFFMYILQTSLTFSVYLFILMQGV
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMFVSELTNAFQGISSKLLPGSFPAVDVAASYGFGSSNAVLSGFAFGLIGQLITIALLVI
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FKNPILIITGFVPVFFDNAAIAVYADKRGGWKAAVALSFISGILQVALGAVAVGLLGLTG
HCCCEEEEECCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYHGNIDLVLPWLPFGYLFKFLGIAGYVLVCIFLLAIPQLQFAKAKDKEAYYRGEAQ
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAGFVKATVGYLILNVGAG
CCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGFISVATTALLIGFGVNILL
CHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNAFGAWAVGIICGLYWAISSNM
HHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKEENLDNLKLPTFLNIFHDTVVAS
EEEHHHHHCCCCCEEECCCCEEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQAFFMYILQTSLTFSVYLFILMQGV
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMFVSELTNAFQGISSKLLPGSFPAVDVAASYGFGSSNAVLSGFAFGLIGQLITIALLVI
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FKNPILIITGFVPVFFDNAAIAVYADKRGGWKAAVALSFISGILQVALGAVAVGLLGLTG
HCCCEEEEECCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYHGNIDLVLPWLPFGYLFKFLGIAGYVLVCIFLLAIPQLQFAKAKDKEAYYRGEAQ
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]