Click here to switch to the map view.

The map label for this gene is truB

Identifier: 71908068

GI number: 71908068

Start: 2650463

End: 2651362

Strand: Reverse

Name: truB

Synonym: Daro_2450

Alternate gene names: 71908068

Gene position: 2651362-2650463 (Counterclockwise)

Preceding gene: 71908069

Following gene: 71908067

Centisome position: 58.9

GC content: 57.78

Gene sequence:

>900_bases
ATGCAATTTAAAAGGGCCTGGAAACAGGTCGATGGCGTTCTCTTGCTCGACAAGCCGCTAGGGATGACCTCCAACGATGC
TCTGCAAAAGGCACGTCGCCTCTTTTCGGCGGCAAAGGGCGGCCATACAGGGACACTGGACCCCCTGGCTACTGGCCTGC
TCCCATTGTGTTTCGGCGAGGCGACCAAGTTTTCGGCCGATTTGCTTGATGCCGACAAGACCTATGAGGCCGTTCTCAAG
CTTGGTGTTACGACTGATTCAGGGGATGCAGAGGGGCAGGTGACGTCGACTGCGATGGTCGATATCCAAAAGGAGCAGGT
TTCTGAAGTCTTGCCCAGATTCGTTGGCGACATCCAGCAGATTCCGCCCATGCATTCGGCCTTGAAACGCAATGGTCGGC
CACTCTATGAATTGGCTCGCCAGGGGATCGAAGTCGAACGCGAGCCGAGGGCGGTTACTATTTTTGCCATCGATTGCCTC
GATTTTTCCGGTGATTTGCTGACGTTGCGCGTTGCTTGCAGCAAGGGAACCTATATTCGTGTGCTGGCCGCTGATATCGG
CAAGGCATTGGGTTGCGGTGCGCATCTGGCAGCGCTCCGCCGAATCGTGGTGGGCGATATACGCCTGGGCAACTCCGTCA
CACTGGCTGAGTTGGAAACCTTGGACGAGGCAGGCCGTATGGAGAGGCTCCTGCCGGTTGATGCCTTGCTGCAGTCTTTG
CCCATCGTCGGCGTCGAGGGGCCGGAAGCGGAACGCTTCCGCCATGGCAATCCGGTGAGCTTGCCGGCCGGGTTGGCCGG
TAAAGCTCGGGTCTATGCCGAGGGGCGATTGATTGGTGTCGGTGAGCCGGGTCATGGTGGTTTGCTTTGGCCGAAAAGGC
TGGTTCAACTGGCTGATTAA

Upstream 100 bases:

>100_bases
TTTGTATATGACAGTTCCATTGAGCGTGGTGCCAGCCTGTCGCTGCTGATTGATCAGGCCAACGCACTTAGCGACCAGAC
GCCGGAAGAGTAATTCCCCA

Downstream 100 bases:

>100_bases
ACTGTTATAATCTTTTACTTTCCGTCTTTGGCGGAATTTTCCCAACGGGCGCCCGCTCTATCAAACTGGGGCAGCGTCGC
ATCAATGAAAGAGAGTTTGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQFKRAWKQVDGVLLLDKPLGMTSNDALQKARRLFSAAKGGHTGTLDPLATGLLPLCFGEATKFSADLLDADKTYEAVLK
LGVTTDSGDAEGQVTSTAMVDIQKEQVSEVLPRFVGDIQQIPPMHSALKRNGRPLYELARQGIEVEREPRAVTIFAIDCL
DFSGDLLTLRVACSKGTYIRVLAADIGKALGCGAHLAALRRIVVGDIRLGNSVTLAELETLDEAGRMERLLPVDALLQSL
PIVGVEGPEAERFRHGNPVSLPAGLAGKARVYAEGRLIGVGEPGHGGLLWPKRLVQLAD

Sequences:

>Translated_299_residues
MQFKRAWKQVDGVLLLDKPLGMTSNDALQKARRLFSAAKGGHTGTLDPLATGLLPLCFGEATKFSADLLDADKTYEAVLK
LGVTTDSGDAEGQVTSTAMVDIQKEQVSEVLPRFVGDIQQIPPMHSALKRNGRPLYELARQGIEVEREPRAVTIFAIDCL
DFSGDLLTLRVACSKGTYIRVLAADIGKALGCGAHLAALRRIVVGDIRLGNSVTLAELETLDEAGRMERLLPVDALLQSL
PIVGVEGPEAERFRHGNPVSLPAGLAGKARVYAEGRLIGVGEPGHGGLLWPKRLVQLAD
>Mature_299_residues
MQFKRAWKQVDGVLLLDKPLGMTSNDALQKARRLFSAAKGGHTGTLDPLATGLLPLCFGEATKFSADLLDADKTYEAVLK
LGVTTDSGDAEGQVTSTAMVDIQKEQVSEVLPRFVGDIQQIPPMHSALKRNGRPLYELARQGIEVEREPRAVTIFAIDCL
DFSGDLLTLRVACSKGTYIRVLAADIGKALGCGAHLAALRRIVVGDIRLGNSVTLAELETLDEAGRMERLLPVDALLQSL
PIVGVEGPEAERFRHGNPVSLPAGLAGKARVYAEGRLIGVGEPGHGGLLWPKRLVQLAD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=249, Percent_Identity=34.5381526104418, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI215599015, Length=194, Percent_Identity=30.9278350515464, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4503337, Length=194, Percent_Identity=30.9278350515464, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI2367200, Length=305, Percent_Identity=45.9016393442623, Blast_Score=263, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17553978, Length=265, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=148, Percent_Identity=41.8918918918919, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=262, Percent_Identity=26.7175572519084, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_DECAR (Q47D96)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285655.1
- ProteinModelPortal:   Q47D96
- SMR:   Q47D96
- STRING:   Q47D96
- GeneID:   3568223
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2450
- NMPDR:   fig|159087.4.peg.2425
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   CLSK2525920
- BioCyc:   DARO159087:DARO_2450-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32048; Mature: 32048

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKRAWKQVDGVLLLDKPLGMTSNDALQKARRLFSAAKGGHTGTLDPLATGLLPLCFGE
CCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
ATKFSADLLDADKTYEAVLKLGVTTDSGDAEGQVTSTAMVDIQKEQVSEVLPRFVGDIQQ
CHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEHHEEEECHHHHHHHHHHHHHHHHH
IPPMHSALKRNGRPLYELARQGIEVEREPRAVTIFAIDCLDFSGDLLTLRVACSKGTYIR
CCCHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCEEE
VLAADIGKALGCGAHLAALRRIVVGDIRLGNSVTLAELETLDEAGRMERLLPVDALLQSL
EEHHHHHHHHCCHHHHHHHHHHHHHCEEECCCEEHHHHHHHHHHHHHHHHCCHHHHHHHC
PIVGVEGPEAERFRHGNPVSLPAGLAGKARVYAEGRLIGVGEPGHGGLLWPKRLVQLAD
CEECCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MQFKRAWKQVDGVLLLDKPLGMTSNDALQKARRLFSAAKGGHTGTLDPLATGLLPLCFGE
CCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
ATKFSADLLDADKTYEAVLKLGVTTDSGDAEGQVTSTAMVDIQKEQVSEVLPRFVGDIQQ
CHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEHHEEEECHHHHHHHHHHHHHHHHH
IPPMHSALKRNGRPLYELARQGIEVEREPRAVTIFAIDCLDFSGDLLTLRVACSKGTYIR
CCCHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCEEE
VLAADIGKALGCGAHLAALRRIVVGDIRLGNSVTLAELETLDEAGRMERLLPVDALLQSL
EEHHHHHHHHCCHHHHHHHHHHHHHCEEECCCEEHHHHHHHHHHHHHHHHCCHHHHHHHC
PIVGVEGPEAERFRHGNPVSLPAGLAGKARVYAEGRLIGVGEPGHGGLLWPKRLVQLAD
CEECCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA