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The map label for this gene is asnB [H]

Identifier: 71908046

GI number: 71908046

Start: 2621765

End: 2623432

Strand: Reverse

Name: asnB [H]

Synonym: Daro_2428

Alternate gene names: 71908046

Gene position: 2623432-2621765 (Counterclockwise)

Preceding gene: 71908047

Following gene: 71908045

Centisome position: 58.28

GC content: 50.36

Gene sequence:

>1668_bases
ATGTTAAAAAATCCAGATATTCTTGTGGTTGGAAAACACAGCTTCAATGACGCGGCTTTGTCTCAAATTGCCAAGTCCAA
AGGTGACGAAGCTGCTTGGCAACAAGCCTTCCAGCGCTTCGGCGTCAAGGCGGCGGAGCACGTATCTGGAGATTTTGCAG
TAGCGCTTAATGAGCCGAATGGCCAGGTCTTTCTGGCAATTGATCGCTTTGCCATACGCAGTCTTTGTTATCGCCAGATT
GGAAATCGACTGCTCTTTGCCGAGCGCGCCGATGAATTGGCGGATGCGAGTACGGAAATTGATCCTCAAGCGATCTTTGA
CTATTTCTACTTTCACGCAATCCCATCCCCGAGGACGATCTTCAAAGGAGTCTATCGGGTACCCCCCGGCCACTATGTTT
TGTTTGAAAATGCTCAATTAACTGTTGCGCCCTATTGGATACCCACTTTCGAGGAGACCAGGAATCAGCCCTTCGAGAAA
CTTCGTGATGAGTTCCGGGACATTCTGCAGCAAGCAACAACTCGCCAATTGGATGGCGGTAAAGCCGCGTGTTTCCTGAG
CGGCGGAACCGATAGCTCTACCGTGGCAGGGATGATTGGCCTCGCTCACGGTGCGCCGGCAGCGACATACTCCATCGGAT
TTGAGGCCGAGGGCTATGACGAAATGGCATTCGCCCGCATTGCCGCCCGCCGTTTCAATACTGAACATCATGAGTATTAC
GTTACTCCAGACGATCTCGTGAGTAGCATTCCGATGGTTGCATCCAGCTACGATCAGCCCTTCGGTAATTCTTCAGCTCT
CCCTGCCTATTACTGCGCGAAGATGGCAAAGGAGGGCGGTGTCTCCAAGATACTGGCCGGTGATGGTGGCGATGAACTCT
TCGGCGGAAATTCGCGTTATGCCAAACAAAGAATTTTCAATAGCTACCGGCATCTCCCGAGCTTCTTGCAAAAAGGACTT
CTTGAGCCGTTATTCAGCCTGCCTGGTATGGATCAAATCCCCTTGCTTAAAAAAGGCAGTAGCTATATTGAGCAGGCTAG
CATTCCGATGCCAGACCGCCTTCAGCTATACAACCTGATTACACGCCTCGGTGTTGATCAGGTGCTGACCCCCGGATTTT
TGCAAGCTATTAACCTAAATGCGCCACTGGGCCTTCAACGAGAGGTTTGGTCCTCTGCAGTTACTGACAGTCTGACCAAC
CGGGAACTGGCATTCGATTGGCGTTTTACGCTGGCGGAAAGTGATTTGCCTAAGGTTTGTGGAACTACTCGCTTGGCTGG
TATCAATGTCGGATTTCCGATGCTTGATGACGAACTGCTAGCGTTTTCATTACGCTTGCCAGTCGAGTACAAACTGAAGG
GGCTGAAGCTGCGCTGGTTCTTCAAGGAGGCACTGCGCGGATTTTTGCCCGACGAAATCATTGTCAAGAAAAAGCAGGGG
TTTGGTCTTCCCTTTGGAGTCTGGCTGAATCGTCACGATGCGTTAAGAAAGCTAGCCAGAAACTCGGTCATGGGATTGGT
TGATCGTGGGGTGCTTCGTGGCGAATTTGTCGATCAACTCTTCAATAAACATCTGCAAGAACATCCTGGCTACTATGGCG
AGATGATCTGGATATCAATGATGCTTGAGCAATGGCTAGTGCATCATCGAGCGAACTATTCGATCTGA

Upstream 100 bases:

>100_bases
GGTCTTCGCGGTGAGGTCGGCTGTTGCGTAATCACAAACGTAGAATCCGGGGGGCTCGTCCACGTTGTTCTTCGGCTCTC
AAACTTAATTGCTTAAATTC

Downstream 100 bases:

>100_bases
AAATTGTGTGACGATTTGCAGAAATGATGTTGAAAAAAATTGATACTAAACTGATATTTCTCTTCGGTATGCCAAGCACC
TCCAAGTAGACAATGATTTA

Product: asparagine synthase (glutamine-hydrolysing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLKNPDILVVGKHSFNDAALSQIAKSKGDEAAWQQAFQRFGVKAAEHVSGDFAVALNEPNGQVFLAIDRFAIRSLCYRQI
GNRLLFAERADELADASTEIDPQAIFDYFYFHAIPSPRTIFKGVYRVPPGHYVLFENAQLTVAPYWIPTFEETRNQPFEK
LRDEFRDILQQATTRQLDGGKAACFLSGGTDSSTVAGMIGLAHGAPAATYSIGFEAEGYDEMAFARIAARRFNTEHHEYY
VTPDDLVSSIPMVASSYDQPFGNSSALPAYYCAKMAKEGGVSKILAGDGGDELFGGNSRYAKQRIFNSYRHLPSFLQKGL
LEPLFSLPGMDQIPLLKKGSSYIEQASIPMPDRLQLYNLITRLGVDQVLTPGFLQAINLNAPLGLQREVWSSAVTDSLTN
RELAFDWRFTLAESDLPKVCGTTRLAGINVGFPMLDDELLAFSLRLPVEYKLKGLKLRWFFKEALRGFLPDEIIVKKKQG
FGLPFGVWLNRHDALRKLARNSVMGLVDRGVLRGEFVDQLFNKHLQEHPGYYGEMIWISMMLEQWLVHHRANYSI

Sequences:

>Translated_555_residues
MLKNPDILVVGKHSFNDAALSQIAKSKGDEAAWQQAFQRFGVKAAEHVSGDFAVALNEPNGQVFLAIDRFAIRSLCYRQI
GNRLLFAERADELADASTEIDPQAIFDYFYFHAIPSPRTIFKGVYRVPPGHYVLFENAQLTVAPYWIPTFEETRNQPFEK
LRDEFRDILQQATTRQLDGGKAACFLSGGTDSSTVAGMIGLAHGAPAATYSIGFEAEGYDEMAFARIAARRFNTEHHEYY
VTPDDLVSSIPMVASSYDQPFGNSSALPAYYCAKMAKEGGVSKILAGDGGDELFGGNSRYAKQRIFNSYRHLPSFLQKGL
LEPLFSLPGMDQIPLLKKGSSYIEQASIPMPDRLQLYNLITRLGVDQVLTPGFLQAINLNAPLGLQREVWSSAVTDSLTN
RELAFDWRFTLAESDLPKVCGTTRLAGINVGFPMLDDELLAFSLRLPVEYKLKGLKLRWFFKEALRGFLPDEIIVKKKQG
FGLPFGVWLNRHDALRKLARNSVMGLVDRGVLRGEFVDQLFNKHLQEHPGYYGEMIWISMMLEQWLVHHRANYSI
>Mature_555_residues
MLKNPDILVVGKHSFNDAALSQIAKSKGDEAAWQQAFQRFGVKAAEHVSGDFAVALNEPNGQVFLAIDRFAIRSLCYRQI
GNRLLFAERADELADASTEIDPQAIFDYFYFHAIPSPRTIFKGVYRVPPGHYVLFENAQLTVAPYWIPTFEETRNQPFEK
LRDEFRDILQQATTRQLDGGKAACFLSGGTDSSTVAGMIGLAHGAPAATYSIGFEAEGYDEMAFARIAARRFNTEHHEYY
VTPDDLVSSIPMVASSYDQPFGNSSALPAYYCAKMAKEGGVSKILAGDGGDELFGGNSRYAKQRIFNSYRHLPSFLQKGL
LEPLFSLPGMDQIPLLKKGSSYIEQASIPMPDRLQLYNLITRLGVDQVLTPGFLQAINLNAPLGLQREVWSSAVTDSLTN
RELAFDWRFTLAESDLPKVCGTTRLAGINVGFPMLDDELLAFSLRLPVEYKLKGLKLRWFFKEALRGFLPDEIIVKKKQG
FGLPFGVWLNRHDALRKLARNSVMGLVDRGVLRGEFVDQLFNKHLQEHPGYYGEMIWISMMLEQWLVHHRANYSI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62294; Mature: 62294

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNPDILVVGKHSFNDAALSQIAKSKGDEAAWQQAFQRFGVKAAEHVSGDFAVALNEPN
CCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEECCC
GQVFLAIDRFAIRSLCYRQIGNRLLFAERADELADASTEIDPQAIFDYFYFHAIPSPRTI
CEEEEEEHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHH
FKGVYRVPPGHYVLFENAQLTVAPYWIPTFEETRNQPFEKLRDEFRDILQQATTRQLDGG
HHHHHCCCCCCEEEEECCEEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KAACFLSGGTDSSTVAGMIGLAHGAPAATYSIGFEAEGYDEMAFARIAARRFNTEHHEYY
CEEEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEE
VTPDDLVSSIPMVASSYDQPFGNSSALPAYYCAKMAKEGGVSKILAGDGGDELFGGNSRY
ECHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHH
AKQRIFNSYRHLPSFLQKGLLEPLFSLPGMDQIPLLKKGSSYIEQASIPMPDRLQLYNLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHH
TRLGVDQVLTPGFLQAINLNAPLGLQREVWSSAVTDSLTNRELAFDWRFTLAESDLPKVC
HHHCHHHHCCCHHHHEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHH
GTTRLAGINVGFPMLDDELLAFSLRLPVEYKLKGLKLRWFFKEALRGFLPDEIIVKKKQG
CCCEEEEEECCCCCCCCHHHHEEECCCCEEEECCEEEHHHHHHHHHCCCCCHHEEEECCC
FGLPFGVWLNRHDALRKLARNSVMGLVDRGVLRGEFVDQLFNKHLQEHPGYYGEMIWISM
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MLEQWLVHHRANYSI
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKNPDILVVGKHSFNDAALSQIAKSKGDEAAWQQAFQRFGVKAAEHVSGDFAVALNEPN
CCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEECCC
GQVFLAIDRFAIRSLCYRQIGNRLLFAERADELADASTEIDPQAIFDYFYFHAIPSPRTI
CEEEEEEHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHH
FKGVYRVPPGHYVLFENAQLTVAPYWIPTFEETRNQPFEKLRDEFRDILQQATTRQLDGG
HHHHHCCCCCCEEEEECCEEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
KAACFLSGGTDSSTVAGMIGLAHGAPAATYSIGFEAEGYDEMAFARIAARRFNTEHHEYY
CEEEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEE
VTPDDLVSSIPMVASSYDQPFGNSSALPAYYCAKMAKEGGVSKILAGDGGDELFGGNSRY
ECHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHH
AKQRIFNSYRHLPSFLQKGLLEPLFSLPGMDQIPLLKKGSSYIEQASIPMPDRLQLYNLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHH
TRLGVDQVLTPGFLQAINLNAPLGLQREVWSSAVTDSLTNRELAFDWRFTLAESDLPKVC
HHHCHHHHCCCHHHHEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHH
GTTRLAGINVGFPMLDDELLAFSLRLPVEYKLKGLKLRWFFKEALRGFLPDEIIVKKKQG
CCCEEEEEECCCCCCCCHHHHEEECCCCEEEECCEEEHHHHHHHHHCCCCCHHEEEECCC
FGLPFGVWLNRHDALRKLARNSVMGLVDRGVLRGEFVDQLFNKHLQEHPGYYGEMIWISM
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MLEQWLVHHRANYSI
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]