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The map label for this gene is cynT [H]

Identifier: 71907717

GI number: 71907717

Start: 2248128

End: 2248763

Strand: Reverse

Name: cynT [H]

Synonym: Daro_2091

Alternate gene names: 71907717

Gene position: 2248763-2248128 (Counterclockwise)

Preceding gene: 71907719

Following gene: 71907716

Centisome position: 49.96

GC content: 59.12

Gene sequence:

>636_bases
GTGCAAAAGGTCATTGAAGGTTTCTTGAAGTTTCAGAAGGACGTCTTTCCGGAAAGAACGCAGCTGTTCAAGCAATTGGC
GCAGCAGCAGAACCCCAAGGTGCTGTTTGTGACCTGTTCGGATAGTCGGGTGGTGCCGGAATTGTTTACACAGCAGGAAC
CCGGCGATCTGTTCGTCATTCGTAACGCGGGCAACATCGTGCCGTCCTACGGGCCAGAGCCGGGCGGTGTTTCGGCAACC
GTCGAGTATGCGGTATCTGTCCTTCAGGTCAGCGATATCGTGATTTGCGGTCATTCGAATTGTGGGGCGATGGCGGCAAT
TTCCAGCTGTCAATGCCTCGATCATCTGCCGGCGGTCGCCCACTGGTTGCGCCACGCGGATTCCGCCAAGGCCATCGTCG
CCTCGGAGACGTACGCGACCCAGCAGGAGAAAGCTGATGCGCTGGTTCGCCAGAACGTGATCGCCCAGCTCGCCAATATC
CGCACGCATCCGTCGGTTGCGCTGGCCCTCGCACAGCGCCGCCTGAACCTGCACGGCTGGGTGTACGACATCGAGAATGG
GGTGATCGATGCCCTCGACGGTGCAAGTGCGAGCTTCGTGCCGCTATCCGCCAATCACCAGGTGGTTGCCACCTAG

Upstream 100 bases:

>100_bases
TATTTCCAGGTCATTGCTCAGAATCGCCCTTGGAACTTTTCTGCCCGCTCCGGCTCTATTGCGGGCATTTCCACATCAGA
TACCCTCCCGGAGTTAAGTC

Downstream 100 bases:

>100_bases
CGCCAACCACCGAGGTGGCTAGTTCCGGCATGAAGCGGTGGCGAAAACGGAATCTCTTCATCACCGTACGCCGCGGCTGC
CTGTTTGCAGGATAAGGATG

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MQKVIEGFLKFQKDVFPERTQLFKQLAQQQNPKVLFVTCSDSRVVPELFTQQEPGDLFVIRNAGNIVPSYGPEPGGVSAT
VEYAVSVLQVSDIVICGHSNCGAMAAISSCQCLDHLPAVAHWLRHADSAKAIVASETYATQQEKADALVRQNVIAQLANI
RTHPSVALALAQRRLNLHGWVYDIENGVIDALDGASASFVPLSANHQVVAT

Sequences:

>Translated_211_residues
MQKVIEGFLKFQKDVFPERTQLFKQLAQQQNPKVLFVTCSDSRVVPELFTQQEPGDLFVIRNAGNIVPSYGPEPGGVSAT
VEYAVSVLQVSDIVICGHSNCGAMAAISSCQCLDHLPAVAHWLRHADSAKAIVASETYATQQEKADALVRQNVIAQLANI
RTHPSVALALAQRRLNLHGWVYDIENGVIDALDGASASFVPLSANHQVVAT
>Mature_211_residues
MQKVIEGFLKFQKDVFPERTQLFKQLAQQQNPKVLFVTCSDSRVVPELFTQQEPGDLFVIRNAGNIVPSYGPEPGGVSAT
VEYAVSVLQVSDIVICGHSNCGAMAAISSCQCLDHLPAVAHWLRHADSAKAIVASETYATQQEKADALVRQNVIAQLANI
RTHPSVALALAQRRLNLHGWVYDIENGVIDALDGASASFVPLSANHQVVAT

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=210, Percent_Identity=69.0476190476191, Blast_Score=322, Evalue=1e-89,
Organism=Escherichia coli, GI1786318, Length=187, Percent_Identity=35.2941176470588, Blast_Score=103, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25147564, Length=224, Percent_Identity=30.3571428571429, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115532990, Length=240, Percent_Identity=25.8333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324292, Length=189, Percent_Identity=30.1587301587302, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645213, Length=245, Percent_Identity=25.7142857142857, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 22846; Mature: 22846

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVIEGFLKFQKDVFPERTQLFKQLAQQQNPKVLFVTCSDSRVVPELFTQQEPGDLFVI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSATVEYAVSVLQVSDIVICGHSNCGAMAAISSCQCLDHLPAVA
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HWLRHADSAKAIVASETYATQQEKADALVRQNVIAQLANIRTHPSVALALAQRRLNLHGW
HHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
VYDIENGVIDALDGASASFVPLSANHQVVAT
EEECCCCEEECCCCCCCCEEEECCCCEEEEC
>Mature Secondary Structure
MQKVIEGFLKFQKDVFPERTQLFKQLAQQQNPKVLFVTCSDSRVVPELFTQQEPGDLFVI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSATVEYAVSVLQVSDIVICGHSNCGAMAAISSCQCLDHLPAVA
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HWLRHADSAKAIVASETYATQQEKADALVRQNVIAQLANIRTHPSVALALAQRRLNLHGW
HHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
VYDIENGVIDALDGASASFVPLSANHQVVAT
EEECCCCEEECCCCCCCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]