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The map label for this gene is pldA [C]

Identifier: 71907716

GI number: 71907716

Start: 2246854

End: 2247885

Strand: Reverse

Name: pldA [C]

Synonym: Daro_2090

Alternate gene names: 71907716

Gene position: 2247885-2246854 (Counterclockwise)

Preceding gene: 71907717

Following gene: 71907715

Centisome position: 49.94

GC content: 58.91

Gene sequence:

>1032_bases
ATGAATAAGAAGATGGTTGTGCTTGCGCTGGCCTTTTTGCCACGTTTGGTCATGTCAGCGGAGCCCCTGGCCGGGTGCGC
TGCCGAAGCGGATGATCGCTTGCGGCTGGCCTGCTATGACAATGCCTACAAGCGATTGAAAGCAGGGAGCGAGACGGTCA
GCGATGCCCAGGTCGATGCGCAGGTGGCGACGAGCAACAGCCAGCGTGAATCGTCAGTCATGTCGAAAACCTGGGAACTG
GGGCCGACCGACAAGCGCAACACCTTTGTCGTTCGAACCTATCTGCCCAACTTCCTGCTTCCGCTTCACTACACCTCGAA
CATCAACCGTACGCCGCATAGTCCGACCCAGCAGGCCGGTGCGGCCAATAGCGGCTACCGCAGTATCGAAGCCAAGCTGC
AAATTTCATTGCGGGCCAAGGTCGTCGAAGACCTTCTTTTGCCGGGCGCCGACCTGTGGGCTGCCTACACCCAGCGCTCG
CTTTGGCAATTGTGGGATGCCGGTGATTCCTCACCATTCCGCAGCACCGATTACCAGCCGGAAATGATCTATGTCGTGCC
GGTACCCGAGAAATTCGGGAAGCTGCCACTGGGCTGGAACCTGCGCATGCTTCAGCTGGGTTTTGCCCACCAGTCGAACG
GACAAAGCGATCCGCTTTCCCGTAGCTGGAACCGGGTCACCATGGGCGCCGGCTTCGAGCACGGCGACTTCACTCTTCAG
ATTCGTGGCAATCAACGGATTAGCTTGCAGGGCAAGGATGACAACCCGGATCTCGCAGATTACATCGGGCGGGGTGAGGT
CAACATGGCCTGGTTGCCAGGTACTTCGACGCTCGGCCTGACCTGGCGAACCAATATGAAGTCGGCCAGTCGCGGCTCGC
TGCAACTTGACTGGACCTACCCGGTATTCGCCAATCAACCGACCGGACTGCGCTGGTATGCCCAGTTGTTCCATGGCTAT
GGGGAGACCCTGCTAGACTACAACCAGCGGCAGACCAGCCTGGGGCTGGGGTTGACGCTGTTTCAGTTCTGA

Upstream 100 bases:

>100_bases
ACGCGCGGGTGGCTCTGTGCGCCAGCACACAGAGCCGCCGATCCGGAAGGCGTAGCCTTGGCGCCAACTGCCGAAAGGGC
AGGTGTGGCATCGGGGATGT

Downstream 100 bases:

>100_bases
CTCGCTCTTCAATGCGTACCCGATTATCAAGCCGACATCCTGAAAAGCACCGGACAACGCGGATCGGCTGGCTACGGGCG
GCGGTGCTTGGTGCCAACGA

Product: phospholipase A1

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNKKMVVLALAFLPRLVMSAEPLAGCAAEADDRLRLACYDNAYKRLKAGSETVSDAQVDAQVATSNSQRESSVMSKTWEL
GPTDKRNTFVVRTYLPNFLLPLHYTSNINRTPHSPTQQAGAANSGYRSIEAKLQISLRAKVVEDLLLPGADLWAAYTQRS
LWQLWDAGDSSPFRSTDYQPEMIYVVPVPEKFGKLPLGWNLRMLQLGFAHQSNGQSDPLSRSWNRVTMGAGFEHGDFTLQ
IRGNQRISLQGKDDNPDLADYIGRGEVNMAWLPGTSTLGLTWRTNMKSASRGSLQLDWTYPVFANQPTGLRWYAQLFHGY
GETLLDYNQRQTSLGLGLTLFQF

Sequences:

>Translated_343_residues
MNKKMVVLALAFLPRLVMSAEPLAGCAAEADDRLRLACYDNAYKRLKAGSETVSDAQVDAQVATSNSQRESSVMSKTWEL
GPTDKRNTFVVRTYLPNFLLPLHYTSNINRTPHSPTQQAGAANSGYRSIEAKLQISLRAKVVEDLLLPGADLWAAYTQRS
LWQLWDAGDSSPFRSTDYQPEMIYVVPVPEKFGKLPLGWNLRMLQLGFAHQSNGQSDPLSRSWNRVTMGAGFEHGDFTLQ
IRGNQRISLQGKDDNPDLADYIGRGEVNMAWLPGTSTLGLTWRTNMKSASRGSLQLDWTYPVFANQPTGLRWYAQLFHGY
GETLLDYNQRQTSLGLGLTLFQF
>Mature_343_residues
MNKKMVVLALAFLPRLVMSAEPLAGCAAEADDRLRLACYDNAYKRLKAGSETVSDAQVDAQVATSNSQRESSVMSKTWEL
GPTDKRNTFVVRTYLPNFLLPLHYTSNINRTPHSPTQQAGAANSGYRSIEAKLQISLRAKVVEDLLLPGADLWAAYTQRS
LWQLWDAGDSSPFRSTDYQPEMIYVVPVPEKFGKLPLGWNLRMLQLGFAHQSNGQSDPLSRSWNRVTMGAGFEHGDFTLQ
IRGNQRISLQGKDDNPDLADYIGRGEVNMAWLPGTSTLGLTWRTNMKSASRGSLQLDWTYPVFANQPTGLRWYAQLFHGY
GETLLDYNQRQTSLGLGLTLFQF

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=257, Percent_Identity=33.4630350194552, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 38393; Mature: 38393

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKMVVLALAFLPRLVMSAEPLAGCAAEADDRLRLACYDNAYKRLKAGSETVSDAQVDA
CCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHCCE
QVATSNSQRESSVMSKTWELGPTDKRNTFVVRTYLPNFLLPLHYTSNINRTPHSPTQQAG
EEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHCC
AANSGYRSIEAKLQISLRAKVVEDLLLPGADLWAAYTQRSLWQLWDAGDSSPFRSTDYQP
CCCCCCEEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EMIYVVPVPEKFGKLPLGWNLRMLQLGFAHQSNGQSDPLSRSWNRVTMGAGFEHGDFTLQ
CEEEEEECCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
IRGNQRISLQGKDDNPDLADYIGRGEVNMAWLPGTSTLGLTWRTNMKSASRGSLQLDWTY
EECCEEEEEECCCCCCCHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEC
PVFANQPTGLRWYAQLFHGYGETLLDYNQRQTSLGLGLTLFQF
CEECCCCCCHHHHHHHHHHHCHHHHCCCCCHHHHCCCEEEECC
>Mature Secondary Structure
MNKKMVVLALAFLPRLVMSAEPLAGCAAEADDRLRLACYDNAYKRLKAGSETVSDAQVDA
CCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHCCE
QVATSNSQRESSVMSKTWELGPTDKRNTFVVRTYLPNFLLPLHYTSNINRTPHSPTQQAG
EEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHCC
AANSGYRSIEAKLQISLRAKVVEDLLLPGADLWAAYTQRSLWQLWDAGDSSPFRSTDYQP
CCCCCCEEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EMIYVVPVPEKFGKLPLGWNLRMLQLGFAHQSNGQSDPLSRSWNRVTMGAGFEHGDFTLQ
CEEEEEECCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
IRGNQRISLQGKDDNPDLADYIGRGEVNMAWLPGTSTLGLTWRTNMKSASRGSLQLDWTY
EECCEEEEEECCCCCCCHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEC
PVFANQPTGLRWYAQLFHGYGETLLDYNQRQTSLGLGLTLFQF
CEECCCCCCHHHHHHHHHHHCHHHHCCCCCHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]