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The map label for this gene is glpK

Identifier: 71907551

GI number: 71907551

Start: 2066879

End: 2068387

Strand: Reverse

Name: glpK

Synonym: Daro_1922

Alternate gene names: 71907551

Gene position: 2068387-2066879 (Counterclockwise)

Preceding gene: 71907556

Following gene: 71907550

Centisome position: 45.95

GC content: 65.01

Gene sequence:

>1509_bases
ATGCAAGATCGTTCCGACCAGTTCGTTCTCGCGCTTGACCAGGGAACGACCAGCACACGCGCCATCCTGTTCGGGCGGAA
GGGCGATATTCATGGCGTCGCCCAGCAGGAAATCACCCAGCATTACCCGCAACCCGGGTGGGTCGAGCACGACGCTGAAG
AAATATGGCAGGCGCAGCTTGCCGTGGCGCGAGCGGCACTCCGCGATAACGGCATATTGGCCAAGCAGATCGTCGCTGTC
GGCATGACCAACCAGCGGGAAACGACGGTGCTTTGGGATCGATCCAGCGGTGAACCGCTGCACCGCGCTATCGTCTGGCA
GGATAGACGCACCGCCGGGCTTTGCGACGAATTGACAACGGCCGGCCACGCCGGGCTGTTCCGCGAACGCACTGGTCTCG
TACTCGATGCCTATTTTTCCGGCACCAAGTTAAAGTGGCTGCTCGACCACATTCCCGGCGCTCGCTCCCGGGCCGAGCGT
GGCGAACTGGCTTTCGGCACCATCGACAGCTGGCTGACCTGGAAACTCTCCGGCGGGCGCGCCCATGTCACCGACCCGTC
GAATGCCTCGCGCACCCTGCTCTTCGACATCCACCGCTGCTGCTGGGATGAGGAATTACTGGCCTTGCTCGACATTCCGA
TGGCGCTGCTGCCGCGCATCGTCGACAGCAGCGGCGAAATCGCCACCATCGCCGCCGAATGGCTGGGCGCCGAGATCCCG
CTCAGTGGCATCGCTGGCGACCAGCAGGCGGCCACTTTCGGCCAGGTCTGTCTCCAGCATGGCATGGCCAAGAACACCTA
CGGGACTGGCTGCTTCTTGCTGATGAATACCGGCCAAGCCCCCATGGCGTCCTGCCACCGGCTACTGACCACCATCGGCT
GGCAGCGGCAAGGGAAGACCACTTACCTGCTCGAAGGCAGCGTCTTCATGGGCGGTGCCACAGTACAGTGGTTGCGCGAC
GGCCTCGGGCTGATCTCCAGCGCGGACCAGATCGAGTCGCTGGCAGCGAGCGTCGCGGATAACGGCGGTGTCTACCTGGT
GCCGGCCCACACCGGCCTCGGCGCGCCATACTGGGACCCGTTCGCCCGAGGCGCCTTGTTCGGCATGACTCGCGGCACCA
CCCGCGCCCACATCGCCCGTGCCGCGCTGGAAGCCATCGCATTCCAGAGTGCCGATGTGCTGCAGGCCATGGAAAAGGAT
GCCGGTCAAAGCCTGAGCGAACTTCGCGTCGACGGCGGTGCGGCACGAAACGACCTGCTGATGCAGTTTCAGGCAGATTT
GCTCGGCGTGCCCGTGGTGCGGCCGCAGGTGACCGAAACCACGGCGCTTGGTGCCGCCTATCTGGCTGGACTGGCCGTGG
GTTTCTGGCAAGACGAGGCCGAGCTGACGGCACTGTGGCGGGCCGACCGGCGCTTTGAGCCGTCCATGGCCGAGGACCGG
CGCAGTGCGCTGTTCGCCGACTGGCACCGCGCCGTCGAACGCAGCCTGCATTGGGCAAACGCGACATGA

Upstream 100 bases:

>100_bases
AAACGGCAATCGCCGAAACGCCCCTCTGTTGTCGCTTAACCAGAAAAAAATAGTTGGATAGCCGTCTATACTGGCTGAAC
ACCGGTTGCCGGAGCACCAA

Downstream 100 bases:

>100_bases
TCAACGCACCGCTCAAGCGCGAAGACGGCCTCGCCAAGCTGCGCGACACTGCCCCTTGGGATGTACTGATCATCGGCGGT
GGCGCCAGCGGCCTGGGCGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MQDRSDQFVLALDQGTTSTRAILFGRKGDIHGVAQQEITQHYPQPGWVEHDAEEIWQAQLAVARAALRDNGILAKQIVAV
GMTNQRETTVLWDRSSGEPLHRAIVWQDRRTAGLCDELTTAGHAGLFRERTGLVLDAYFSGTKLKWLLDHIPGARSRAER
GELAFGTIDSWLTWKLSGGRAHVTDPSNASRTLLFDIHRCCWDEELLALLDIPMALLPRIVDSSGEIATIAAEWLGAEIP
LSGIAGDQQAATFGQVCLQHGMAKNTYGTGCFLLMNTGQAPMASCHRLLTTIGWQRQGKTTYLLEGSVFMGGATVQWLRD
GLGLISSADQIESLAASVADNGGVYLVPAHTGLGAPYWDPFARGALFGMTRGTTRAHIARAALEAIAFQSADVLQAMEKD
AGQSLSELRVDGGAARNDLLMQFQADLLGVPVVRPQVTETTALGAAYLAGLAVGFWQDEAELTALWRADRRFEPSMAEDR
RSALFADWHRAVERSLHWANAT

Sequences:

>Translated_502_residues
MQDRSDQFVLALDQGTTSTRAILFGRKGDIHGVAQQEITQHYPQPGWVEHDAEEIWQAQLAVARAALRDNGILAKQIVAV
GMTNQRETTVLWDRSSGEPLHRAIVWQDRRTAGLCDELTTAGHAGLFRERTGLVLDAYFSGTKLKWLLDHIPGARSRAER
GELAFGTIDSWLTWKLSGGRAHVTDPSNASRTLLFDIHRCCWDEELLALLDIPMALLPRIVDSSGEIATIAAEWLGAEIP
LSGIAGDQQAATFGQVCLQHGMAKNTYGTGCFLLMNTGQAPMASCHRLLTTIGWQRQGKTTYLLEGSVFMGGATVQWLRD
GLGLISSADQIESLAASVADNGGVYLVPAHTGLGAPYWDPFARGALFGMTRGTTRAHIARAALEAIAFQSADVLQAMEKD
AGQSLSELRVDGGAARNDLLMQFQADLLGVPVVRPQVTETTALGAAYLAGLAVGFWQDEAELTALWRADRRFEPSMAEDR
RSALFADWHRAVERSLHWANAT
>Mature_502_residues
MQDRSDQFVLALDQGTTSTRAILFGRKGDIHGVAQQEITQHYPQPGWVEHDAEEIWQAQLAVARAALRDNGILAKQIVAV
GMTNQRETTVLWDRSSGEPLHRAIVWQDRRTAGLCDELTTAGHAGLFRERTGLVLDAYFSGTKLKWLLDHIPGARSRAER
GELAFGTIDSWLTWKLSGGRAHVTDPSNASRTLLFDIHRCCWDEELLALLDIPMALLPRIVDSSGEIATIAAEWLGAEIP
LSGIAGDQQAATFGQVCLQHGMAKNTYGTGCFLLMNTGQAPMASCHRLLTTIGWQRQGKTTYLLEGSVFMGGATVQWLRD
GLGLISSADQIESLAASVADNGGVYLVPAHTGLGAPYWDPFARGALFGMTRGTTRAHIARAALEAIAFQSADVLQAMEKD
AGQSLSELRVDGGAARNDLLMQFQADLLGVPVVRPQVTETTALGAAYLAGLAVGFWQDEAELTALWRADRRFEPSMAEDR
RSALFADWHRAVERSLHWANAT

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=510, Percent_Identity=45.2941176470588, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI189409120, Length=506, Percent_Identity=45.0592885375494, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4504007, Length=506, Percent_Identity=45.0592885375494, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=44.8343079922027, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=517, Percent_Identity=34.6228239845261, Blast_Score=335, Evalue=7e-92,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=54.5638945233266, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1790005, Length=468, Percent_Identity=27.7777777777778, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787789, Length=483, Percent_Identity=28.3643892339545, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789987, Length=463, Percent_Identity=25.7019438444924, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=26.4646464646465, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789138, Length=494, Percent_Identity=22.6720647773279, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=43.0830039525692, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=524, Percent_Identity=28.6259541984733, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=32.8859060402685, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321755, Length=486, Percent_Identity=37.6543209876543, Blast_Score=300, Evalue=5e-82,
Organism=Drosophila melanogaster, GI17864214, Length=516, Percent_Identity=43.4108527131783, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=442, Percent_Identity=45.2488687782805, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655555, Length=504, Percent_Identity=38.4920634920635, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24655560, Length=504, Percent_Identity=38.4920634920635, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24655570, Length=504, Percent_Identity=38.4920634920635, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24655574, Length=504, Percent_Identity=38.4920634920635, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24655565, Length=504, Percent_Identity=38.4920634920635, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.5863377609108, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=34.412955465587, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24656367, Length=517, Percent_Identity=31.9148936170213, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21357867, Length=532, Percent_Identity=31.203007518797, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=34.0974212034384, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=30.6896551724138, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_DECAR (Q47ER3)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285138.1
- ProteinModelPortal:   Q47ER3
- SMR:   Q47ER3
- STRING:   Q47ER3
- GeneID:   3569579
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1922
- NMPDR:   fig|159087.4.peg.1934
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK2525863
- BioCyc:   DARO159087:DARO_1922-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54713; Mature: 54713

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 16-16 BINDING 20-20 BINDING 86-86 BINDING 138-138 BINDING 247-247 BINDING 269-269 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRSDQFVLALDQGTTSTRAILFGRKGDIHGVAQQEITQHYPQPGWVEHDAEEIWQAQL
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVARAALRDNGILAKQIVAVGMTNQRETTVLWDRSSGEPLHRAIVWQDRRTAGLCDELTT
HHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEECCCCCCCCHHHHHH
AGHAGLFRERTGLVLDAYFSGTKLKWLLDHIPGARSRAERGELAFGTIDSWLTWKLSGGR
CCCCCHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCE
AHVTDPSNASRTLLFDIHRCCWDEELLALLDIPMALLPRIVDSSGEIATIAAEWLGAEIP
EEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEHHHCCCCCC
LSGIAGDQQAATFGQVCLQHGMAKNTYGTGCFLLMNTGQAPMASCHRLLTTIGWQRQGKT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCE
TYLLEGSVFMGGATVQWLRDGLGLISSADQIESLAASVADNGGVYLVPAHTGLGAPYWDP
EEEEECCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
FARGALFGMTRGTTRAHIARAALEAIAFQSADVLQAMEKDAGQSLSELRVDGGAARNDLL
HHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
MQFQADLLGVPVVRPQVTETTALGAAYLAGLAVGFWQDEAELTALWRADRRFEPSMAEDR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
RSALFADWHRAVERSLHWANAT
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQDRSDQFVLALDQGTTSTRAILFGRKGDIHGVAQQEITQHYPQPGWVEHDAEEIWQAQL
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVARAALRDNGILAKQIVAVGMTNQRETTVLWDRSSGEPLHRAIVWQDRRTAGLCDELTT
HHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEECCCCCCCCHHHHHH
AGHAGLFRERTGLVLDAYFSGTKLKWLLDHIPGARSRAERGELAFGTIDSWLTWKLSGGR
CCCCCHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCE
AHVTDPSNASRTLLFDIHRCCWDEELLALLDIPMALLPRIVDSSGEIATIAAEWLGAEIP
EEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEHHHCCCCCC
LSGIAGDQQAATFGQVCLQHGMAKNTYGTGCFLLMNTGQAPMASCHRLLTTIGWQRQGKT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCE
TYLLEGSVFMGGATVQWLRDGLGLISSADQIESLAASVADNGGVYLVPAHTGLGAPYWDP
EEEEECCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
FARGALFGMTRGTTRAHIARAALEAIAFQSADVLQAMEKDAGQSLSELRVDGGAARNDLL
HHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
MQFQADLLGVPVVRPQVTETTALGAAYLAGLAVGFWQDEAELTALWRADRRFEPSMAEDR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
RSALFADWHRAVERSLHWANAT
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA