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The map label for this gene is sbcD [H]

Identifier: 71907549

GI number: 71907549

Start: 2063927

End: 2065171

Strand: Reverse

Name: sbcD [H]

Synonym: Daro_1920

Alternate gene names: 71907549

Gene position: 2065171-2063927 (Counterclockwise)

Preceding gene: 71907550

Following gene: 71907548

Centisome position: 45.88

GC content: 66.51

Gene sequence:

>1245_bases
ATGCGCATTCTCCACACCTCCGACTGGCACCTTGGCCAGCACTTCATGGGCAAGAGCCGGCAGGCCGAGCATCAGGCGCT
AATCGTCTGGCTGCTCGAACAGGTGGAAACGCAGGCAGTGGATGCCGTGCTGATCGCCGGCGACATCTTCGACACCGGCA
CGCCACCCAGCTACGCCCGCGAGTTGTACAACCAGCTGGTCGGGCAACTCTACAAGGCCGGCGTGCCGCTGCTGGTGCTC
GGCGGCAACCACGATTCGCCGGCCACGCTGGGCGAAAGCCGCGAACTGCTCGCCCACCTGGGCACCACCGTCATCGGCGC
AACGCACACCGACCCGGCCACGCAGGTCATCGTCCTGCCGCAACGCAACGGCGAACCCGGCTGCATCGTCTGCGCCATTC
CCTTCGTCCGGCCGCGCGACGTGCTGCAAAGCCAAGCTGGGCAAAGCGCCGAAGACAAGCAGCTGTCGCTGCAAACCGCC
ATCCAGGAACATTACAGCGCCGTGTTCGCCGCCGCCGTTGAACGCCAGCAGGCACTGGCCGCCCAACTCGGCCCGAATTT
CGGCCGCCAGCTGCCGATCATCGCCACCGGCCACCTGACCACCGTCGGCGCCAGCACCAGCGAATCGGTCCGCGAAATCT
ACGTCGGCGCCCTCGAAGCCTTTCCGACCACCGCCTTCCCGCCGGCTGCCTACATCGCGCTCGGCCATATCCACCGGCCA
CAGAAAGTCGGCGGACTGGACCATATCCGCTACTGCGGCTCGCCGATCCCGCTCAGCTTCGACGAAGCGAAACAAACCAA
GGAAATGCTGCTCGTCGATCTCGACAGCGATGGCCTCAAGGCCGTCACCGTGCTGCCTGTGCCGCGCTTCCAGGCGCTGG
TCGCCGTCAGCGGCAATCTCGAATCACTGGCCGGCGCAATCGGCGCCGCAGCCGCCGAAGGCACGCGCGAATGCCCGGCC
TGGCTTGAAGTCACCGTCGCCGAAGACGATTACCTGGCCGACCTGCCGGCCCGCATCGAAGCGTTGACCGAAGGCTGGCC
GGTCGAGGTGCTGCGCATCCGCCGCCAGCGCGGCAATGCCACGGCCCGACTGGCCGCCGAGGCCCGCGAAACGCTGGACG
AACTCAGCCCGCACGACGTTTTTGCCCGCCGCTTGCAACAGGAAGAACTCGGCGAAGAAATGCAGTTGGCCCTCAACGAA
CGCTACCGCGCCGTCGTTGCCGGACTGCAGGGAGAGGAAGCATGA

Upstream 100 bases:

>100_bases
TGCCAGCCCAACCGACGCAAAGCGCCAGAGGTGGCCGAACGAGGCGATCACAAGCGCCTTGGCGGTTTAAACTTCGATCT
TTCCATCTCAGCAGCCTGGC

Downstream 100 bases:

>100_bases
AAATCCTTAGCCTGCGCCTGAAGAACCTCAATTCGCTGAAGGGCGAATGGACCATCGACTTCACCAAAGCCCCCTTCACC
GACAACTGCCTGTTCGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRILHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVETQAVDAVLIAGDIFDTGTPPSYARELYNQLVGQLYKAGVPLLVL
GGNHDSPATLGESRELLAHLGTTVIGATHTDPATQVIVLPQRNGEPGCIVCAIPFVRPRDVLQSQAGQSAEDKQLSLQTA
IQEHYSAVFAAAVERQQALAAQLGPNFGRQLPIIATGHLTTVGASTSESVREIYVGALEAFPTTAFPPAAYIALGHIHRP
QKVGGLDHIRYCGSPIPLSFDEAKQTKEMLLVDLDSDGLKAVTVLPVPRFQALVAVSGNLESLAGAIGAAAAEGTRECPA
WLEVTVAEDDYLADLPARIEALTEGWPVEVLRIRRQRGNATARLAAEARETLDELSPHDVFARRLQQEELGEEMQLALNE
RYRAVVAGLQGEEA

Sequences:

>Translated_414_residues
MRILHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVETQAVDAVLIAGDIFDTGTPPSYARELYNQLVGQLYKAGVPLLVL
GGNHDSPATLGESRELLAHLGTTVIGATHTDPATQVIVLPQRNGEPGCIVCAIPFVRPRDVLQSQAGQSAEDKQLSLQTA
IQEHYSAVFAAAVERQQALAAQLGPNFGRQLPIIATGHLTTVGASTSESVREIYVGALEAFPTTAFPPAAYIALGHIHRP
QKVGGLDHIRYCGSPIPLSFDEAKQTKEMLLVDLDSDGLKAVTVLPVPRFQALVAVSGNLESLAGAIGAAAAEGTRECPA
WLEVTVAEDDYLADLPARIEALTEGWPVEVLRIRRQRGNATARLAAEARETLDELSPHDVFARRLQQEELGEEMQLALNE
RYRAVVAGLQGEEA
>Mature_414_residues
MRILHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVETQAVDAVLIAGDIFDTGTPPSYARELYNQLVGQLYKAGVPLLVL
GGNHDSPATLGESRELLAHLGTTVIGATHTDPATQVIVLPQRNGEPGCIVCAIPFVRPRDVLQSQAGQSAEDKQLSLQTA
IQEHYSAVFAAAVERQQALAAQLGPNFGRQLPIIATGHLTTVGASTSESVREIYVGALEAFPTTAFPPAAYIALGHIHRP
QKVGGLDHIRYCGSPIPLSFDEAKQTKEMLLVDLDSDGLKAVTVLPVPRFQALVAVSGNLESLAGAIGAAAAEGTRECPA
WLEVTVAEDDYLADLPARIEALTEGWPVEVLRIRRQRGNATARLAAEARETLDELSPHDVFARRLQQEELGEEMQLALNE
RYRAVVAGLQGEEA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=413, Percent_Identity=50.363196125908, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44703; Mature: 44703

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVETQAVDAVLIAGDIFDTGTPPSYAR
CEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
ELYNQLVGQLYKAGVPLLVLGGNHDSPATLGESRELLAHLGTTVIGATHTDPATQVIVLP
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCHHHEECCCCCCCCEEEEEE
QRNGEPGCIVCAIPFVRPRDVLQSQAGQSAEDKQLSLQTAIQEHYSAVFAAAVERQQALA
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLGPNFGRQLPIIATGHLTTVGASTSESVREIYVGALEAFPTTAFPPAAYIALGHIHRP
HHHCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
QKVGGLDHIRYCGSPIPLSFDEAKQTKEMLLVDLDSDGLKAVTVLPVPRFQALVAVSGNL
HHCCCCHHHHHCCCCCCCCHHHHHHCCEEEEEEECCCCCEEEEEECCCCCEEEEEECCCH
ESLAGAIGAAAAEGTRECPAWLEVTVAEDDYLADLPARIEALTEGWPVEVLRIRRQRGNA
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC
TARLAAEARETLDELSPHDVFARRLQQEELGEEMQLALNERYRAVVAGLQGEEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVETQAVDAVLIAGDIFDTGTPPSYAR
CEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
ELYNQLVGQLYKAGVPLLVLGGNHDSPATLGESRELLAHLGTTVIGATHTDPATQVIVLP
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCHHHEECCCCCCCCEEEEEE
QRNGEPGCIVCAIPFVRPRDVLQSQAGQSAEDKQLSLQTAIQEHYSAVFAAAVERQQALA
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLGPNFGRQLPIIATGHLTTVGASTSESVREIYVGALEAFPTTAFPPAAYIALGHIHRP
HHHCCCCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
QKVGGLDHIRYCGSPIPLSFDEAKQTKEMLLVDLDSDGLKAVTVLPVPRFQALVAVSGNL
HHCCCCHHHHHCCCCCCCCHHHHHHCCEEEEEEECCCCCEEEEEECCCCCEEEEEECCCH
ESLAGAIGAAAAEGTRECPAWLEVTVAEDDYLADLPARIEALTEGWPVEVLRIRRQRGNA
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHCCCCHHHHHHHHHCCCC
TARLAAEARETLDELSPHDVFARRLQQEELGEEMQLALNERYRAVVAGLQGEEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]