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The map label for this gene is parE [H]

Identifier: 71907496

GI number: 71907496

Start: 2005726

End: 2007714

Strand: Reverse

Name: parE [H]

Synonym: Daro_1867

Alternate gene names: 71907496

Gene position: 2007714-2005726 (Counterclockwise)

Preceding gene: 71907501

Following gene: 71907495

Centisome position: 44.6

GC content: 61.34

Gene sequence:

>1989_bases
TTGCGGTCGACCGACATTATCTCCCTAATTCCAGAGCGCATGACCGACTACTCCGCCGCCTCAATCCGCGTCCTCAAGGA
CCTCGAACCCGTCAAGGAACGTCCCGGGATGTACACCAGGACGACCTGCCCGACTCATATCGTGCAGGAAGTCATCGACA
ACGCCGCCGACGAGGCACTGGCCGGTTTCGCCAAAAAGATCGCAGTCGCCATCCGTGCCGACGGGGTCATCGAGGTTTCC
GACAACGGACGCGGCATTCCGGTCGAGATACACCCGGAAGAAGGCCGGCCGGCGGTCGAACTGGTCTTCTGCAAGCTGCA
CGCCGGCGGCAAGTTCAACAAGAAAGAATCGGGCAACGCCTACCGTTTCTCGGGCGGCCTGCACGGCGTCGGCGTTTCGG
TGACCAATGCGCTGTCGATCCGGCTGGAAGTCGAGATCAAGCGCGGTGGCGGCATTCATCACATCGCCTTCGGCGCCGGC
TTTGTCACCGAACCCCTGAGCCGGATCGGCGACTGCGGCCAGCGCAACACCGGGACCACGGTCCGCATCCAGCCTGACCC
AAAGTATTTCGATTCGCCCAAGGTCAATCTCGGCCAACTGGAGCATCTGCTGCGCTCAAAAGCCGTGCTGATGCCCAATG
TCACGGTCGAGCTGGACATCGAAGGCCAGGACAAGAAGGTCTGGTGCTACGAAAACGGCATGGCCGACTACCTGAACGAA
ATGATGGTCGGCACGCCGGTAGCACCGATTTTCGTTGGTGAAAAATACGTTGAGACTGCCAATGGCTTTGCCGTCGGCGA
AGGTGCGACCTGGGCGCTGGCCTGGTTCGAGGAAGGTGGCGGCAAGCCGGAAAGCTACGTCAACCTGATCCCGACGCTGG
ATGGCGGCACCCATGAAGCCGGGTTGAAGGCTGGCGTCTTCGAGGCGATCAAGGCCTTTGCCGATCACCACGCCATCCTG
CCGGCCAAGGTCAAACTGGCGCAGGAAGACGTTTGCGGCAAGATGACCTATCTGCTCTCGGCCAAGGTGCTCGACCCGCA
ATTCCAGGGCCAGACCAAGGAAAAACTGACCAGCCGCGATGCCTACAAGCTGGTCTCGCAGATGGTGCGCGACCCGTTCG
AGCTATGGCTGAACAGCCATGCCGATTTCGGCAAGAAGATCGCCGAACTGGCCGTCAAGGCGGCGCAGAACCGGATGAAA
TCGACGCAGAAGATCGAGAAGAAGAAGTCGTCCGGCATCGCCACCCTGCCCGGCAAACTGACCGACTGCGAATCCGACGA
CATCTTGCGCAACGAAATCTTCCTGGTCGAGGGCGATTCGGCCGGCGGCTCGGCCAAACAGGGCCGCGACAAGGAAACCC
AGGCCGTGCTGCCGCTGCGCGGCAAGGTGCTGAACACCTGGGAAGTCGAGCGCGACCAGCTGTTCAAGAACAATGAAGTA
CACGACATTTCCGTCGCCGTTGGTGTTGATCCGCACGGCATCGAACAAATCGACAGCGTCGATGTCACCGGCCTGCGCTA
CGGCCGCATCATCGTCATGTCCGACGCCGACGTCGATGGCTCGCACATTCAGGTGTTGCTGTTCACCCTATTCCTCAAGC
ACTTCCCGGCCCTCGTCGAGCGCGGCCACATCCACGTCGCCCAGCCACCGCTGTTCCGCGTCGACGTTGAAGCCCGCGGC
CACACCCGCAAGGTCTATTGCCTGGACGAAGCCGAGAAGGAACAGACGCTGCAGAAGCTAGCCGACGAAGGCGTCTCGGA
AAACAAGATCACGGTCTCCCGCTTCAAGGGCCTCGGCGAAATGAACCCGGACCAACTGTGGGAAACCACGCTCTGCCCGG
ACACCCGCCGCCTTGTGCCGTTCCAGGCCAGCCGCGAAACCATCAAGGAAATGGCCACCACCTTCACGCTGCTGATGGGC
AAGGGCGAAGCCTCCGGCCGCCGGGCCTGGATGGAAAAGGACGGCGGATTGGTTGAGGCTGACGTTTGA

Upstream 100 bases:

>100_bases
CGCGATCTGGGCAATGTTCATTGGGCTTCCTTTTCTGAATATGCGTTTGATTCGTTGCGGCAGCCGCGCTGCCGGTTAAA
ATCCGGGTCCGCGCCGCTGC

Downstream 100 bases:

>100_bases
GCGATTTTCCGCCTGACCGGGCATTTTCTGGCCTTACCGTGGTCTGATTCGCATCTGAATCACGCCGAACCCCAAGCTGT
TCAAGCAAGCGAAGCCGATG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MRSTDIISLIPERMTDYSAASIRVLKDLEPVKERPGMYTRTTCPTHIVQEVIDNAADEALAGFAKKIAVAIRADGVIEVS
DNGRGIPVEIHPEEGRPAVELVFCKLHAGGKFNKKESGNAYRFSGGLHGVGVSVTNALSIRLEVEIKRGGGIHHIAFGAG
FVTEPLSRIGDCGQRNTGTTVRIQPDPKYFDSPKVNLGQLEHLLRSKAVLMPNVTVELDIEGQDKKVWCYENGMADYLNE
MMVGTPVAPIFVGEKYVETANGFAVGEGATWALAWFEEGGGKPESYVNLIPTLDGGTHEAGLKAGVFEAIKAFADHHAIL
PAKVKLAQEDVCGKMTYLLSAKVLDPQFQGQTKEKLTSRDAYKLVSQMVRDPFELWLNSHADFGKKIAELAVKAAQNRMK
STQKIEKKKSSGIATLPGKLTDCESDDILRNEIFLVEGDSAGGSAKQGRDKETQAVLPLRGKVLNTWEVERDQLFKNNEV
HDISVAVGVDPHGIEQIDSVDVTGLRYGRIIVMSDADVDGSHIQVLLFTLFLKHFPALVERGHIHVAQPPLFRVDVEARG
HTRKVYCLDEAEKEQTLQKLADEGVSENKITVSRFKGLGEMNPDQLWETTLCPDTRRLVPFQASRETIKEMATTFTLLMG
KGEASGRRAWMEKDGGLVEADV

Sequences:

>Translated_662_residues
MRSTDIISLIPERMTDYSAASIRVLKDLEPVKERPGMYTRTTCPTHIVQEVIDNAADEALAGFAKKIAVAIRADGVIEVS
DNGRGIPVEIHPEEGRPAVELVFCKLHAGGKFNKKESGNAYRFSGGLHGVGVSVTNALSIRLEVEIKRGGGIHHIAFGAG
FVTEPLSRIGDCGQRNTGTTVRIQPDPKYFDSPKVNLGQLEHLLRSKAVLMPNVTVELDIEGQDKKVWCYENGMADYLNE
MMVGTPVAPIFVGEKYVETANGFAVGEGATWALAWFEEGGGKPESYVNLIPTLDGGTHEAGLKAGVFEAIKAFADHHAIL
PAKVKLAQEDVCGKMTYLLSAKVLDPQFQGQTKEKLTSRDAYKLVSQMVRDPFELWLNSHADFGKKIAELAVKAAQNRMK
STQKIEKKKSSGIATLPGKLTDCESDDILRNEIFLVEGDSAGGSAKQGRDKETQAVLPLRGKVLNTWEVERDQLFKNNEV
HDISVAVGVDPHGIEQIDSVDVTGLRYGRIIVMSDADVDGSHIQVLLFTLFLKHFPALVERGHIHVAQPPLFRVDVEARG
HTRKVYCLDEAEKEQTLQKLADEGVSENKITVSRFKGLGEMNPDQLWETTLCPDTRRLVPFQASRETIKEMATTFTLLMG
KGEASGRRAWMEKDGGLVEADV
>Mature_662_residues
MRSTDIISLIPERMTDYSAASIRVLKDLEPVKERPGMYTRTTCPTHIVQEVIDNAADEALAGFAKKIAVAIRADGVIEVS
DNGRGIPVEIHPEEGRPAVELVFCKLHAGGKFNKKESGNAYRFSGGLHGVGVSVTNALSIRLEVEIKRGGGIHHIAFGAG
FVTEPLSRIGDCGQRNTGTTVRIQPDPKYFDSPKVNLGQLEHLLRSKAVLMPNVTVELDIEGQDKKVWCYENGMADYLNE
MMVGTPVAPIFVGEKYVETANGFAVGEGATWALAWFEEGGGKPESYVNLIPTLDGGTHEAGLKAGVFEAIKAFADHHAIL
PAKVKLAQEDVCGKMTYLLSAKVLDPQFQGQTKEKLTSRDAYKLVSQMVRDPFELWLNSHADFGKKIAELAVKAAQNRMK
STQKIEKKKSSGIATLPGKLTDCESDDILRNEIFLVEGDSAGGSAKQGRDKETQAVLPLRGKVLNTWEVERDQLFKNNEV
HDISVAVGVDPHGIEQIDSVDVTGLRYGRIIVMSDADVDGSHIQVLLFTLFLKHFPALVERGHIHVAQPPLFRVDVEARG
HTRKVYCLDEAEKEQTLQKLADEGVSENKITVSRFKGLGEMNPDQLWETTLCPDTRRLVPFQASRETIKEMATTFTLLMG
KGEASGRRAWMEKDGGLVEADV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=655, Percent_Identity=25.4961832061069, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI19913408, Length=553, Percent_Identity=25.8589511754069, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=49.8461538461538, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=40.8273381294964, Blast_Score=351, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI212645845, Length=570, Percent_Identity=25.9649122807018, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535065, Length=549, Percent_Identity=25.3187613843352, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=657, Percent_Identity=24.5053272450533, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17136538, Length=644, Percent_Identity=23.6024844720497, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 72696; Mature: 72696

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTDIISLIPERMTDYSAASIRVLKDLEPVKERPGMYTRTTCPTHIVQEVIDNAADEAL
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHH
AGFAKKIAVAIRADGVIEVSDNGRGIPVEIHPEEGRPAVELVFCKLHAGGKFNKKESGNA
HHHHHHHEEEEECCCEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCE
YRFSGGLHGVGVSVTNALSIRLEVEIKRGGGIHHIAFGAGFVTEPLSRIGDCGQRNTGTT
EEECCCCCCCCEEEEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCE
VRIQPDPKYFDSPKVNLGQLEHLLRSKAVLMPNVTVELDIEGQDKKVWCYENGMADYLNE
EEECCCCCCCCCCCCCHHHHHHHHHCCEEECCCEEEEEEECCCCCEEEEEECCHHHHHHH
MMVGTPVAPIFVGEKYVETANGFAVGEGATWALAWFEEGGGKPESYVNLIPTLDGGTHEA
HHHCCCCCCEEECCHHHHCCCCEEECCCCEEEEEEEECCCCCCCHHEEEEECCCCCCCCC
GLKAGVFEAIKAFADHHAILPAKVKLAQEDVCGKMTYLLSAKVLDPQFQGQTKEKLTSRD
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AYKLVSQMVRDPFELWLNSHADFGKKIAELAVKAAQNRMKSTQKIEKKKSSGIATLPGKL
HHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TDCESDDILRNEIFLVEGDSAGGSAKQGRDKETQAVLPLRGKVLNTWEVERDQLFKNNEV
CCCCCCCCEECEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECEEECCHHHHHCCCCC
HDISVAVGVDPHGIEQIDSVDVTGLRYGRIIVMSDADVDGSHIQVLLFTLFLKHFPALVE
EEEEEEECCCCCCCHHHCCCCCCEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RGHIHVAQPPLFRVDVEARGHTRKVYCLDEAEKEQTLQKLADEGVSENKITVSRFKGLGE
CCCEEECCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEHHHHCCCCC
MNPDQLWETTLCPDTRRLVPFQASRETIKEMATTFTLLMGKGEASGRRAWMEKDGGLVEA
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCEEEE
DV
CC
>Mature Secondary Structure
MRSTDIISLIPERMTDYSAASIRVLKDLEPVKERPGMYTRTTCPTHIVQEVIDNAADEAL
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHH
AGFAKKIAVAIRADGVIEVSDNGRGIPVEIHPEEGRPAVELVFCKLHAGGKFNKKESGNA
HHHHHHHEEEEECCCEEEECCCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCE
YRFSGGLHGVGVSVTNALSIRLEVEIKRGGGIHHIAFGAGFVTEPLSRIGDCGQRNTGTT
EEECCCCCCCCEEEEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCE
VRIQPDPKYFDSPKVNLGQLEHLLRSKAVLMPNVTVELDIEGQDKKVWCYENGMADYLNE
EEECCCCCCCCCCCCCHHHHHHHHHCCEEECCCEEEEEEECCCCCEEEEEECCHHHHHHH
MMVGTPVAPIFVGEKYVETANGFAVGEGATWALAWFEEGGGKPESYVNLIPTLDGGTHEA
HHHCCCCCCEEECCHHHHCCCCEEECCCCEEEEEEEECCCCCCCHHEEEEECCCCCCCCC
GLKAGVFEAIKAFADHHAILPAKVKLAQEDVCGKMTYLLSAKVLDPQFQGQTKEKLTSRD
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AYKLVSQMVRDPFELWLNSHADFGKKIAELAVKAAQNRMKSTQKIEKKKSSGIATLPGKL
HHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TDCESDDILRNEIFLVEGDSAGGSAKQGRDKETQAVLPLRGKVLNTWEVERDQLFKNNEV
CCCCCCCCEECEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEECEEECCHHHHHCCCCC
HDISVAVGVDPHGIEQIDSVDVTGLRYGRIIVMSDADVDGSHIQVLLFTLFLKHFPALVE
EEEEEEECCCCCCCHHHCCCCCCEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RGHIHVAQPPLFRVDVEARGHTRKVYCLDEAEKEQTLQKLADEGVSENKITVSRFKGLGE
CCCEEECCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEHHHHCCCCC
MNPDQLWETTLCPDTRRLVPFQASRETIKEMATTFTLLMGKGEASGRRAWMEKDGGLVEA
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCEEEE
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]