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The map label for this gene is tig

Identifier: 71907349

GI number: 71907349

Start: 1847131

End: 1848435

Strand: Reverse

Name: tig

Synonym: Daro_1719

Alternate gene names: 71907349

Gene position: 1848435-1847131 (Counterclockwise)

Preceding gene: 71907352

Following gene: 71907348

Centisome position: 41.07

GC content: 58.7

Gene sequence:

>1305_bases
ATGGAAGCAACCACTGCACAAGCTAACGAACTTGAACGCCGCGTCGATCTCTCCATCGCCATCGCCGATGTCGAGAAGGA
AATGGAACAGCGCCTGAAGCGCATGGGCAAGAACATGAAGGTGCCCGGCTTCCGCCCGGGCAAGGTACCGTTCAACATCG
TCAAGCAGCAGTATGGCGACCAGGCTCGCCACGAAGTGCTGTCCGAAGAGCTGGATCGCGTTTTCGGCGAAACCGTCACC
GAAAAGAAAATGCGCGTTGCCGGCTACCCGCGCATCGAGCCGAAGACCACCGAAAGCAACTCGCACCTCGAGTTTTCGGC
CATCTTCGAGGTTTACCCGGAATTCACTCCGGGCGATCTGTCAGGTTCCGAAGTCGAACGCCCGGTCCTCGAAGTCAGCG
CAGCTGAAGTCGACAAGACCCTCGACATTCTGCGCAAGCAGCGCGTTTCCTACGCTGACGCCGACCGCGCCGCCGCCAAG
GAAGACCGCGTCGTCATCGACTTCACCGGCAAGAAGGACGGCGTCCCGTTCCCGGGCGGCCAGGCCAATGATTACCCGTT
CGTTCTCGGCCAGGGCATGATGCTGCCCGATTTCGAAAATGCCGTTGAAGGTGCCAAGGCTGGCGAAACCAAGACTTTTG
ACCTGACCTTCCCGGCTGACTACCACGCCAAGGACCTGGCTGGCCAGACAGTCCAGTTCGACATCACCGTCAAGCAGGTT
CAGGCGCCGGTTCTGCCGGAACTGGATGCAGAATTTGCAACCAGCATGGGCATCGCCGATGGCGACGTGACCAAGATGCG
CGCCGAGATCGAAGCCAACCTGAAGCGCGAAGTGAAGCGCCGCATCGAAGGCAAGCTGAAGGACCAGGTCATGGAAGCCT
TGCTCAAGGCCAACCCGATCACCGTGCCGGTTGCTCTGGTCGACATGGAAATCCAGCGCCTGATGCAAGCTGCTCGTCAG
GACATGGAACAGCGCGGCATGAAGGTCAAGGACATGCCGATCCAGCCCGAGTGGTTTGCTGACCAGGCTAAGCGCCGCGT
CACACTTGGCCTGATCCTTGCTGAAGTGGTCAAGACGGAAAAGCTTCAGGCTAGCCCGGAACAGGTTCGTACGATGGTCG
AAGAAACCGCCCAGAGCTACGAGCACCCGGAAGAAGTGATTCGCTGGTATTACGCCCAGCCGCAGCGTCTGCAGGAAGTC
GAAGGCGTCGCCATCGAGAACAATGTAGTCGAATGGGTCCTGAGCAAAGCCAAGGTCACCGAAAAGGCTGCGGTTTTTGA
TGAATTGATGGGTCAGAAGCAGTAA

Upstream 100 bases:

>100_bases
CATATACCCCGGAGAATAGGCGGAAGCATCCGGCAGCGTACCGGCCGTGATATTATTGTCAGCTTTATTTTTTTCGGCTC
CGTAGTCACAAGGAATTCTC

Downstream 100 bases:

>100_bases
TCTGGACCTTGTCGCGAAAGCGACAATCGTCTTGAATGTAACCAACAAGCAACACACAAGGGCTGACATGAATATCGACA
ACGCAAGCAACTGGGATCCG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MEATTAQANELERRVDLSIAIADVEKEMEQRLKRMGKNMKVPGFRPGKVPFNIVKQQYGDQARHEVLSEELDRVFGETVT
EKKMRVAGYPRIEPKTTESNSHLEFSAIFEVYPEFTPGDLSGSEVERPVLEVSAAEVDKTLDILRKQRVSYADADRAAAK
EDRVVIDFTGKKDGVPFPGGQANDYPFVLGQGMMLPDFENAVEGAKAGETKTFDLTFPADYHAKDLAGQTVQFDITVKQV
QAPVLPELDAEFATSMGIADGDVTKMRAEIEANLKREVKRRIEGKLKDQVMEALLKANPITVPVALVDMEIQRLMQAARQ
DMEQRGMKVKDMPIQPEWFADQAKRRVTLGLILAEVVKTEKLQASPEQVRTMVEETAQSYEHPEEVIRWYYAQPQRLQEV
EGVAIENNVVEWVLSKAKVTEKAAVFDELMGQKQ

Sequences:

>Translated_434_residues
MEATTAQANELERRVDLSIAIADVEKEMEQRLKRMGKNMKVPGFRPGKVPFNIVKQQYGDQARHEVLSEELDRVFGETVT
EKKMRVAGYPRIEPKTTESNSHLEFSAIFEVYPEFTPGDLSGSEVERPVLEVSAAEVDKTLDILRKQRVSYADADRAAAK
EDRVVIDFTGKKDGVPFPGGQANDYPFVLGQGMMLPDFENAVEGAKAGETKTFDLTFPADYHAKDLAGQTVQFDITVKQV
QAPVLPELDAEFATSMGIADGDVTKMRAEIEANLKREVKRRIEGKLKDQVMEALLKANPITVPVALVDMEIQRLMQAARQ
DMEQRGMKVKDMPIQPEWFADQAKRRVTLGLILAEVVKTEKLQASPEQVRTMVEETAQSYEHPEEVIRWYYAQPQRLQEV
EGVAIENNVVEWVLSKAKVTEKAAVFDELMGQKQ
>Mature_434_residues
MEATTAQANELERRVDLSIAIADVEKEMEQRLKRMGKNMKVPGFRPGKVPFNIVKQQYGDQARHEVLSEELDRVFGETVT
EKKMRVAGYPRIEPKTTESNSHLEFSAIFEVYPEFTPGDLSGSEVERPVLEVSAAEVDKTLDILRKQRVSYADADRAAAK
EDRVVIDFTGKKDGVPFPGGQANDYPFVLGQGMMLPDFENAVEGAKAGETKTFDLTFPADYHAKDLAGQTVQFDITVKQV
QAPVLPELDAEFATSMGIADGDVTKMRAEIEANLKREVKRRIEGKLKDQVMEALLKANPITVPVALVDMEIQRLMQAARQ
DMEQRGMKVKDMPIQPEWFADQAKRRVTLGLILAEVVKTEKLQASPEQVRTMVEETAQSYEHPEEVIRWYYAQPQRLQEV
EGVAIENNVVEWVLSKAKVTEKAAVFDELMGQKQ

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=433, Percent_Identity=42.7251732101617, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_DECAR (Q47FB5)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284936.1
- ProteinModelPortal:   Q47FB5
- SMR:   Q47FB5
- STRING:   Q47FB5
- GeneID:   3568537
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1719
- NMPDR:   fig|159087.4.peg.1732
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   DARO159087:DARO_1719-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48861; Mature: 48861

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATTAQANELERRVDLSIAIADVEKEMEQRLKRMGKNMKVPGFRPGKVPFNIVKQQYGD
CCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
QARHEVLSEELDRVFGETVTEKKMRVAGYPRIEPKTTESNSHLEFSAIFEVYPEFTPGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
SGSEVERPVLEVSAAEVDKTLDILRKQRVSYADADRAAAKEDRVVIDFTGKKDGVPFPGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCC
QANDYPFVLGQGMMLPDFENAVEGAKAGETKTFDLTFPADYHAKDLAGQTVQFDITVKQV
CCCCCCEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHCCCEEEEEEEHHHH
QAPVLPELDAEFATSMGIADGDVTKMRAEIEANLKREVKRRIEGKLKDQVMEALLKANPI
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVPVALVDMEIQRLMQAARQDMEQRGMKVKDMPIQPEWFADQAKRRVTLGLILAEVVKTE
EEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLQASPEQVRTMVEETAQSYEHPEEVIRWYYAQPQRLQEVEGVAIENNVVEWVLSKAKVT
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCEEECHHHHHHHHHHHHHH
EKAAVFDELMGQKQ
HHHHHHHHHHCCCC
>Mature Secondary Structure
MEATTAQANELERRVDLSIAIADVEKEMEQRLKRMGKNMKVPGFRPGKVPFNIVKQQYGD
CCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
QARHEVLSEELDRVFGETVTEKKMRVAGYPRIEPKTTESNSHLEFSAIFEVYPEFTPGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
SGSEVERPVLEVSAAEVDKTLDILRKQRVSYADADRAAAKEDRVVIDFTGKKDGVPFPGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCC
QANDYPFVLGQGMMLPDFENAVEGAKAGETKTFDLTFPADYHAKDLAGQTVQFDITVKQV
CCCCCCEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHCCCEEEEEEEHHHH
QAPVLPELDAEFATSMGIADGDVTKMRAEIEANLKREVKRRIEGKLKDQVMEALLKANPI
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVPVALVDMEIQRLMQAARQDMEQRGMKVKDMPIQPEWFADQAKRRVTLGLILAEVVKTE
EEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLQASPEQVRTMVEETAQSYEHPEEVIRWYYAQPQRLQEVEGVAIENNVVEWVLSKAKVT
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCEEECHHHHHHHHHHHHHH
EKAAVFDELMGQKQ
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA