Click here to switch to the map view.

The map label for this gene is cbiF [H]

Identifier: 71907323

GI number: 71907323

Start: 1821545

End: 1822354

Strand: Reverse

Name: cbiF [H]

Synonym: Daro_1693

Alternate gene names: 71907323

Gene position: 1822354-1821545 (Counterclockwise)

Preceding gene: 71907324

Following gene: 71907322

Centisome position: 40.49

GC content: 64.57

Gene sequence:

>810_bases
ATGGCCGGGAAAATCTGGTTTGTCGGCGCCGGCCCGGGCGATCCCGACCTGATCACGGTCAAGGGGCGCAAGCTGCTCGA
AGCATCCGGCGCCGTGCTCTTTGCCGGCTCGCTGGTCGATCAGGCCGCGACGCTGTTCGCGCCGGACGGCTGCGATATCC
GCGACTCCAAGGATATGACGCTGGACGAGATGACTGCGTGGTTGATCGAGCGCGCCCAGCTTCACGAAACCGTCGTTCGC
CTGCAAACCGGTGATCCGGGACTCTATGGCGCGCTGATCGAAATGACTCAGCCGCTGACCGCCGCCGGCATCGAATGGGC
CGTGGTACCCGGCGTTTCTTCGGCCATGGCCTCGATGGCTGCGGCCGGTGAAACACTGACGCTACCGGAAGTCACCCAGA
CCGTCATCCTGACCCGCGTCGCCGGTCGTACGCCGATGCCGCCGGGTGAAGATCTCGAATTGCTGGCCGCGCACAAGACG
ACCTTGTGCATCTATCTGTCGATCACCCTGCTGCACAAGGTTCAGGAGGCCCTGCGGGCGGCCGGCTGGGTCGACGATGC
GCCGATTCTGGTCGTCCAGAAAGCGAGTTGGCCGGGGCAGGAAAAAATCATTCGCGGCACGCTGGCCGACATCAAGCAAA
AGTGCCAGGCCGAGAAAGTCGGCAGCCAGGCCATGATCGTCGCCAGCCCAACGCTGGGCGCGGCCGACTGGCCAGACCTG
ATCCGTTCCAAGCTCTACGACCCCGCCTTTACGCACCGCTTCCGCAAGGCAAGCCCGGCCTCTGCCGTCACCCCGGAGAT
CAACCCATGA

Upstream 100 bases:

>100_bases
GCCGCAAGTCTGTTGGCCATGTTGGCAGCGACCAATTACCCAAAGGAAAGCTCATGCGAAATTACCTGAAAATTGCTGCT
CACCGTCGGAGAACCTGCTG

Downstream 100 bases:

>100_bases
ACACTGAAACCACCATCCTGCTGGTCGGCCACGGCTCGCGCGGCCGGGAAGGCAACAAGGAAACGATCAATTTTGCTGCC
CAGTGGCGCGAACGCCACCC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MAGKIWFVGAGPGDPDLITVKGRKLLEASGAVLFAGSLVDQAATLFAPDGCDIRDSKDMTLDEMTAWLIERAQLHETVVR
LQTGDPGLYGALIEMTQPLTAAGIEWAVVPGVSSAMASMAAAGETLTLPEVTQTVILTRVAGRTPMPPGEDLELLAAHKT
TLCIYLSITLLHKVQEALRAAGWVDDAPILVVQKASWPGQEKIIRGTLADIKQKCQAEKVGSQAMIVASPTLGAADWPDL
IRSKLYDPAFTHRFRKASPASAVTPEINP

Sequences:

>Translated_269_residues
MAGKIWFVGAGPGDPDLITVKGRKLLEASGAVLFAGSLVDQAATLFAPDGCDIRDSKDMTLDEMTAWLIERAQLHETVVR
LQTGDPGLYGALIEMTQPLTAAGIEWAVVPGVSSAMASMAAAGETLTLPEVTQTVILTRVAGRTPMPPGEDLELLAAHKT
TLCIYLSITLLHKVQEALRAAGWVDDAPILVVQKASWPGQEKIIRGTLADIKQKCQAEKVGSQAMIVASPTLGAADWPDL
IRSKLYDPAFTHRFRKASPASAVTPEINP
>Mature_268_residues
AGKIWFVGAGPGDPDLITVKGRKLLEASGAVLFAGSLVDQAATLFAPDGCDIRDSKDMTLDEMTAWLIERAQLHETVVRL
QTGDPGLYGALIEMTQPLTAAGIEWAVVPGVSSAMASMAAAGETLTLPEVTQTVILTRVAGRTPMPPGEDLELLAAHKTT
LCIYLSITLLHKVQEALRAAGWVDDAPILVVQKASWPGQEKIIRGTLADIKQKCQAEKVGSQAMIVASPTLGAADWPDLI
RSKLYDPAFTHRFRKASPASAVTPEINP

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=29.7071129707113, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28632; Mature: 28501

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKIWFVGAGPGDPDLITVKGRKLLEASGAVLFAGSLVDQAATLFAPDGCDIRDSKDMT
CCCEEEEEECCCCCCCEEEECCCEEEHHCCCEEEEHHHHHHHHHEECCCCCCCCCCCCCC
LDEMTAWLIERAQLHETVVRLQTGDPGLYGALIEMTQPLTAAGIEWAVVPGVSSAMASMA
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCHHHCCCCEEEECCHHHHHHHHH
AAGETLTLPEVTQTVILTRVAGRTPMPPGEDLELLAAHKTTLCIYLSITLLHKVQEALRA
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCEEEEHHHHHHHHHHHHHHHH
AGWVDDAPILVVQKASWPGQEKIIRGTLADIKQKCQAEKVGSQAMIVASPTLGAADWPDL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IRSKLYDPAFTHRFRKASPASAVTPEINP
HHHHCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGKIWFVGAGPGDPDLITVKGRKLLEASGAVLFAGSLVDQAATLFAPDGCDIRDSKDMT
CCEEEEEECCCCCCCEEEECCCEEEHHCCCEEEEHHHHHHHHHEECCCCCCCCCCCCCC
LDEMTAWLIERAQLHETVVRLQTGDPGLYGALIEMTQPLTAAGIEWAVVPGVSSAMASMA
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCHHHCCCCEEEECCHHHHHHHHH
AAGETLTLPEVTQTVILTRVAGRTPMPPGEDLELLAAHKTTLCIYLSITLLHKVQEALRA
HCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCEEEEHHHHHHHHHHHHHHHH
AGWVDDAPILVVQKASWPGQEKIIRGTLADIKQKCQAEKVGSQAMIVASPTLGAADWPDL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
IRSKLYDPAFTHRFRKASPASAVTPEINP
HHHHCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]