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The map label for this gene is nreB [H]

Identifier: 71907205

GI number: 71907205

Start: 1686438

End: 1687559

Strand: Reverse

Name: nreB [H]

Synonym: Daro_1573

Alternate gene names: 71907205

Gene position: 1687559-1686438 (Counterclockwise)

Preceding gene: 71907206

Following gene: 71907204

Centisome position: 37.49

GC content: 58.38

Gene sequence:

>1122_bases
ATGAACACCATCATCGCCGAACGCCCGGACATCCTGATTGTGGACGACACAGCAGCATCGCTGGATCTCCTTAGCGACCT
TCTCGCCCAAGCCGGTTATGCCGTCCGTCCAACCCAGGATCCGCGCATGGCCTTGCGCTCCGCCCTGGCGAAGACTCCGG
AATTGATCCTGCTGGACGTCCGGATGCCGGGCATGGACGGTTATGAGCTATGCCAGCGTTTGAAAGACAACCCGAAGACC
CGTGAAATCCCTGTCATCTTCCTGTCGGCATTGCGTGAAACGGAAGACAAGGTACGCGGCTTCTCACTCGGAGCCGTCGA
TTACATCGCAAAACCATTCCAGCCTGAAGAAGTACTGGCCCGCGTCCGTACCCATATCGAATTGAGACGCCTGCAGTGTC
GCCTTGAGGAGCGAGTGGCCGAGCGGACCGAACAACTGCGCCAATCCAGTGACGAACTTCGCGCCTCCCGGGAACGCCTC
CAGGAGTTGGCCGCTTTCCTGCAAAGCGTACGCGAGGAAGAACGAACGGCGATCGCCCGCGAACTGCACGATGAGTTGGG
GCAAGCCCTCACCGCCCTGCGCATCGACCTGGCCTGGATAAATCGACAATGCAAAACACTGGGTCCAGAGGTCCAAGCGC
GCGCCGATGCGTCCTGTGCCATTGTCGAACGAACCATTGATGCAGTTCGGCGGATTTCCGAAGGTCTCCGTCCGGGGATG
CTCGATGTTCTCGGACTCACCGCCGCCATTGAATATTTGGTCGAACAGTTTGCCAAACGCAATGACATTCCCTGCTATCT
GACCAGCGACCGCGACGAATTTGCGATCGATGATCGGGTCTCAATTGCCGTATTCCGCCTGATCCAGGAGGCGCTGACCA
ACGTCGTCCGGCACGCTTCGGCCACTCGTGTCGACATCACCCTTAGCCAGGACAACAATACAATCTGCGTCGACATTCGT
GATAACGGCATCGGCCTTGCGACAGGCGAACCCAAGCAGGGGTTCGGCCTGCTCGGTATGCGCGAACGTGTCACAATGCT
TGGCGGCACGATCGACATTGACGGTAGCCGGGGGACTCGAATCCTCGCCCGCTTTCCGTTATTCAAGGGAGAAAAGTCAT
GA

Upstream 100 bases:

>100_bases
TCGCCCCCGAGACCGCCAGAGCGTTAACCGAGTTGTGCCGTGAGCGCCGCTACCAATCGCTCTGGGACCTGCTGGGCATA
CTGGAACAAGAGGATTGACC

Downstream 100 bases:

>100_bases
TCAGAACATTGATCGCCGACGACCACGACATACTTCGTATCGGCCTCAAGCACATACTCTCGGACACCGGAGATATCGTC
GTCACCGCCGAAGCGAGCAA

Product: response regulator receiver:ATP-binding region, ATPase-like:histidine kinase, dimerization and phosphoacceptor region

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNTIIAERPDILIVDDTAASLDLLSDLLAQAGYAVRPTQDPRMALRSALAKTPELILLDVRMPGMDGYELCQRLKDNPKT
REIPVIFLSALRETEDKVRGFSLGAVDYIAKPFQPEEVLARVRTHIELRRLQCRLEERVAERTEQLRQSSDELRASRERL
QELAAFLQSVREEERTAIARELHDELGQALTALRIDLAWINRQCKTLGPEVQARADASCAIVERTIDAVRRISEGLRPGM
LDVLGLTAAIEYLVEQFAKRNDIPCYLTSDRDEFAIDDRVSIAVFRLIQEALTNVVRHASATRVDITLSQDNNTICVDIR
DNGIGLATGEPKQGFGLLGMRERVTMLGGTIDIDGSRGTRILARFPLFKGEKS

Sequences:

>Translated_373_residues
MNTIIAERPDILIVDDTAASLDLLSDLLAQAGYAVRPTQDPRMALRSALAKTPELILLDVRMPGMDGYELCQRLKDNPKT
REIPVIFLSALRETEDKVRGFSLGAVDYIAKPFQPEEVLARVRTHIELRRLQCRLEERVAERTEQLRQSSDELRASRERL
QELAAFLQSVREEERTAIARELHDELGQALTALRIDLAWINRQCKTLGPEVQARADASCAIVERTIDAVRRISEGLRPGM
LDVLGLTAAIEYLVEQFAKRNDIPCYLTSDRDEFAIDDRVSIAVFRLIQEALTNVVRHASATRVDITLSQDNNTICVDIR
DNGIGLATGEPKQGFGLLGMRERVTMLGGTIDIDGSRGTRILARFPLFKGEKS
>Mature_373_residues
MNTIIAERPDILIVDDTAASLDLLSDLLAQAGYAVRPTQDPRMALRSALAKTPELILLDVRMPGMDGYELCQRLKDNPKT
REIPVIFLSALRETEDKVRGFSLGAVDYIAKPFQPEEVLARVRTHIELRRLQCRLEERVAERTEQLRQSSDELRASRERL
QELAAFLQSVREEERTAIARELHDELGQALTALRIDLAWINRQCKTLGPEVQARADASCAIVERTIDAVRRISEGLRPGM
LDVLGLTAAIEYLVEQFAKRNDIPCYLTSDRDEFAIDDRVSIAVFRLIQEALTNVVRHASATRVDITLSQDNNTICVDIR
DNGIGLATGEPKQGFGLLGMRERVTMLGGTIDIDGSRGTRILARFPLFKGEKS

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=228, Percent_Identity=36.8421052631579, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788812, Length=217, Percent_Identity=32.7188940092166, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082012, Length=179, Percent_Identity=34.0782122905028, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786599, Length=131, Percent_Identity=38.9312977099237, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=40.3361344537815, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788394, Length=133, Percent_Identity=37.593984962406, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=35.8333333333333, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787474, Length=221, Percent_Identity=25.7918552036199, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786784, Length=114, Percent_Identity=35.0877192982456, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=33.6206896551724, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=122, Percent_Identity=33.6065573770492, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790552, Length=113, Percent_Identity=37.1681415929204, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787375, Length=113, Percent_Identity=32.7433628318584, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=174, Percent_Identity=29.3103448275862, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786911, Length=121, Percent_Identity=36.3636363636364, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=30.1724137931034, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41805; Mature: 41805

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIIAERPDILIVDDTAASLDLLSDLLAQAGYAVRPTQDPRMALRSALAKTPELILLDV
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHCCCCEEEEEE
RMPGMDGYELCQRLKDNPKTREIPVIFLSALRETEDKVRGFSLGAVDYIAKPFQPEEVLA
ECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
RVRTHIELRRLQCRLEERVAERTEQLRQSSDELRASRERLQELAAFLQSVREEERTAIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELHDELGQALTALRIDLAWINRQCKTLGPEVQARADASCAIVERTIDAVRRISEGLRPGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
LDVLGLTAAIEYLVEQFAKRNDIPCYLTSDRDEFAIDDRVSIAVFRLIQEALTNVVRHAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATRVDITLSQDNNTICVDIRDNGIGLATGEPKQGFGLLGMRERVTMLGGTIDIDGSRGTR
CCEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCE
ILARFPLFKGEKS
EEEECCCCCCCCC
>Mature Secondary Structure
MNTIIAERPDILIVDDTAASLDLLSDLLAQAGYAVRPTQDPRMALRSALAKTPELILLDV
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHCCCCEEEEEE
RMPGMDGYELCQRLKDNPKTREIPVIFLSALRETEDKVRGFSLGAVDYIAKPFQPEEVLA
ECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
RVRTHIELRRLQCRLEERVAERTEQLRQSSDELRASRERLQELAAFLQSVREEERTAIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELHDELGQALTALRIDLAWINRQCKTLGPEVQARADASCAIVERTIDAVRRISEGLRPGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
LDVLGLTAAIEYLVEQFAKRNDIPCYLTSDRDEFAIDDRVSIAVFRLIQEALTNVVRHAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATRVDITLSQDNNTICVDIRDNGIGLATGEPKQGFGLLGMRERVTMLGGTIDIDGSRGTR
CCEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCE
ILARFPLFKGEKS
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]