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The map label for this gene is mltF [H]

Identifier: 71906936

GI number: 71906936

Start: 1411728

End: 1413263

Strand: Reverse

Name: mltF [H]

Synonym: Daro_1301

Alternate gene names: 71906936

Gene position: 1413263-1411728 (Counterclockwise)

Preceding gene: 71906939

Following gene: 71906932

Centisome position: 31.4

GC content: 57.55

Gene sequence:

>1536_bases
ATGTACTGGATAAAACGACTATTACTGTTGACAGCATCCCTGATCGCCGCCGGTTGCGGCGAGGTTGGCCCATTGCCATT
TCCGACACCAGCCCAGCATGATCTCGTCGTCCTCACCCGTCCCGGTCTGCTCACTTATTCGACCGATGACAACGGCAACC
CGGCCGGCATCGAATACGATCTGACACAAGCCTTCGCCCAGGAACTCGGTGTCGGCGTCAGATATGTCGTCGTAGCGCCG
GAAGAACTGGAAGCCCAGCTTACCGAAAGCAAGTATCACCTTGCCGCTGCCTGGCTCTCGCCAATGGCCAACAGCGCCAT
CCAGTCGACACCTGCCATTTTTCAGACACGAGACATTCTTGCCCAGCACGAGGCATCGTTGGCGCTCACTGAACGCTCGC
AACTGACCAGCAAGACGGTGCATGTCATGGCCGGCACCCGCCAGGCGAAAACGCTGGCCAGCCTGGCCAAGGAAATACCG
GGACTCAAGGTCGTCGAAGTCGGCCAGGGCGACATCCTTGATCTGCTGGAAAAACTTGGCAATCGTCAGATCGAGTACGT
CGCTATCGACGAGGGCTTTGAGGACATTGCCAATCAGTACGTTCCCAACCTGCGTACCACGCTAGCGCTGAGCGAAACCC
GTCCGGTAGTCTGGCTGCTCGGCCAGCACCCAAATGCAGAACTCAAGGCTCGCCTCGACACCTTCATCGAGCGAGTGCAA
CACGATGGCACACTGGCCCGACTGGAAGACCGCTACCTCGGCCATGTCCGGCGCCTAGAACAAGGCGACATCATCAAATT
TCTTGGTGAGATAGAAACCACGCTCCCCAAATTCCGCAAGTACTTCCAGGCAGCCCAAGCCCTGACCGGCATCGACTGGC
GATTGATTGCGGCAGTGGCCTACCACGAATCGCATTGGGATCCGAACGCAACCAGCTATACCAATGTGCGCGGGATCATG
ATGCTGACCGAAGATACGGCCGACCACCTGGAGGTCAGCAACCGGCTCGACCCCAGCGAAAGCATCCTGGCCGGCGCCCG
CTATATCAATATGCTGAAGGAACAGTTGCCGGAAGAAACCGAGGAGCCGGATCGCACTTGGCTGGCGCTGGCTGCCTACA
ATATTGGCCCCGGCCACTTCAATGCGGCACGGACGCTGGCCAAACAATTGAAAGCCGACCCAAACACCTGGTACGAGATG
AAGCGGATCTTGCCCAAGCTTGCGCAGCCGAAGGTTTACCAGCAATTGAAGACTGGTCGAGCTCGGGGTGGTGAAGCAGT
GATCCTGGTGGAAAATATCCGCAGCTATTACGACATTCTGCAGCGCAATACCGCCCCCTTCGGACCGACGCCAGCTGCTG
CAGAAAGTCTAAAACGGCTGGTCGCGGAGGTTGAAGGACGTCGAAAAACCTACGTCAGGAAGATGGCTGCCTCAAAGGCC
ATCAATGCCACCGGCACAGATACCTCAACAGCCGCATTCAGGCTGCCGACCATCGAATCAGGCAAATACCCAGCCCGACC
GGCAGAAAACGAATAA

Upstream 100 bases:

>100_bases
GATTCGAACCCACGGTACCTTGCAGTACGCCTGATTTCGAGTCAGGTACATTCGACCACTCTGCCACCTCTCCGCAGCGG
CCGGGATAATAGCACAACTG

Downstream 100 bases:

>100_bases
GGGGTTTCCGCCCGCGCCGGAGCGCGAGCGGCGATCGATTCCGATCAGGCGGCCGAGAGAACCTCAAGCCCGCCCATATA
CGGACGCAGCACAGCAGGAA

Product: lytic transglycosylase, catalytic

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MYWIKRLLLLTASLIAAGCGEVGPLPFPTPAQHDLVVLTRPGLLTYSTDDNGNPAGIEYDLTQAFAQELGVGVRYVVVAP
EELEAQLTESKYHLAAAWLSPMANSAIQSTPAIFQTRDILAQHEASLALTERSQLTSKTVHVMAGTRQAKTLASLAKEIP
GLKVVEVGQGDILDLLEKLGNRQIEYVAIDEGFEDIANQYVPNLRTTLALSETRPVVWLLGQHPNAELKARLDTFIERVQ
HDGTLARLEDRYLGHVRRLEQGDIIKFLGEIETTLPKFRKYFQAAQALTGIDWRLIAAVAYHESHWDPNATSYTNVRGIM
MLTEDTADHLEVSNRLDPSESILAGARYINMLKEQLPEETEEPDRTWLALAAYNIGPGHFNAARTLAKQLKADPNTWYEM
KRILPKLAQPKVYQQLKTGRARGGEAVILVENIRSYYDILQRNTAPFGPTPAAAESLKRLVAEVEGRRKTYVRKMAASKA
INATGTDTSTAAFRLPTIESGKYPARPAENE

Sequences:

>Translated_511_residues
MYWIKRLLLLTASLIAAGCGEVGPLPFPTPAQHDLVVLTRPGLLTYSTDDNGNPAGIEYDLTQAFAQELGVGVRYVVVAP
EELEAQLTESKYHLAAAWLSPMANSAIQSTPAIFQTRDILAQHEASLALTERSQLTSKTVHVMAGTRQAKTLASLAKEIP
GLKVVEVGQGDILDLLEKLGNRQIEYVAIDEGFEDIANQYVPNLRTTLALSETRPVVWLLGQHPNAELKARLDTFIERVQ
HDGTLARLEDRYLGHVRRLEQGDIIKFLGEIETTLPKFRKYFQAAQALTGIDWRLIAAVAYHESHWDPNATSYTNVRGIM
MLTEDTADHLEVSNRLDPSESILAGARYINMLKEQLPEETEEPDRTWLALAAYNIGPGHFNAARTLAKQLKADPNTWYEM
KRILPKLAQPKVYQQLKTGRARGGEAVILVENIRSYYDILQRNTAPFGPTPAAAESLKRLVAEVEGRRKTYVRKMAASKA
INATGTDTSTAAFRLPTIESGKYPARPAENE
>Mature_511_residues
MYWIKRLLLLTASLIAAGCGEVGPLPFPTPAQHDLVVLTRPGLLTYSTDDNGNPAGIEYDLTQAFAQELGVGVRYVVVAP
EELEAQLTESKYHLAAAWLSPMANSAIQSTPAIFQTRDILAQHEASLALTERSQLTSKTVHVMAGTRQAKTLASLAKEIP
GLKVVEVGQGDILDLLEKLGNRQIEYVAIDEGFEDIANQYVPNLRTTLALSETRPVVWLLGQHPNAELKARLDTFIERVQ
HDGTLARLEDRYLGHVRRLEQGDIIKFLGEIETTLPKFRKYFQAAQALTGIDWRLIAAVAYHESHWDPNATSYTNVRGIM
MLTEDTADHLEVSNRLDPSESILAGARYINMLKEQLPEETEEPDRTWLALAAYNIGPGHFNAARTLAKQLKADPNTWYEM
KRILPKLAQPKVYQQLKTGRARGGEAVILVENIRSYYDILQRNTAPFGPTPAAAESLKRLVAEVEGRRKTYVRKMAASKA
INATGTDTSTAAFRLPTIESGKYPARPAENE

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=412, Percent_Identity=34.4660194174757, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 56718; Mature: 56718

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWIKRLLLLTASLIAAGCGEVGPLPFPTPAQHDLVVLTRPGLLTYSTDDNGNPAGIEYD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCEEHH
LTQAFAQELGVGVRYVVVAPEELEAQLTESKYHLAAAWLSPMANSAIQSTPAIFQTRDIL
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AQHEASLALTERSQLTSKTVHVMAGTRQAKTLASLAKEIPGLKVVEVGQGDILDLLEKLG
HHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHC
NRQIEYVAIDEGFEDIANQYVPNLRTTLALSETRPVVWLLGQHPNAELKARLDTFIERVQ
CCEEEEEEECCCHHHHHHHHCCCHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HDGTLARLEDRYLGHVRRLEQGDIIKFLGEIETTLPKFRKYFQAAQALTGIDWRLIAAVA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YHESHWDPNATSYTNVRGIMMLTEDTADHLEVSNRLDPSESILAGARYINMLKEQLPEET
HHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
EEPDRTWLALAAYNIGPGHFNAARTLAKQLKADPNTWYEMKRILPKLAQPKVYQQLKTGR
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCC
ARGGEAVILVENIRSYYDILQRNTAPFGPTPAAAESLKRLVAEVEGRRKTYVRKMAASKA
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
INATGTDTSTAAFRLPTIESGKYPARPAENE
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYWIKRLLLLTASLIAAGCGEVGPLPFPTPAQHDLVVLTRPGLLTYSTDDNGNPAGIEYD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCEEHH
LTQAFAQELGVGVRYVVVAPEELEAQLTESKYHLAAAWLSPMANSAIQSTPAIFQTRDIL
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AQHEASLALTERSQLTSKTVHVMAGTRQAKTLASLAKEIPGLKVVEVGQGDILDLLEKLG
HHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHC
NRQIEYVAIDEGFEDIANQYVPNLRTTLALSETRPVVWLLGQHPNAELKARLDTFIERVQ
CCEEEEEEECCCHHHHHHHHCCCHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HDGTLARLEDRYLGHVRRLEQGDIIKFLGEIETTLPKFRKYFQAAQALTGIDWRLIAAVA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YHESHWDPNATSYTNVRGIMMLTEDTADHLEVSNRLDPSESILAGARYINMLKEQLPEET
HHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
EEPDRTWLALAAYNIGPGHFNAARTLAKQLKADPNTWYEMKRILPKLAQPKVYQQLKTGR
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCC
ARGGEAVILVENIRSYYDILQRNTAPFGPTPAAAESLKRLVAEVEGRRKTYVRKMAASKA
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
INATGTDTSTAAFRLPTIESGKYPARPAENE
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA