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The map label for this gene is rnfB [H]

Identifier: 71906801

GI number: 71906801

Start: 1267027

End: 1267569

Strand: Reverse

Name: rnfB [H]

Synonym: Daro_1162

Alternate gene names: 71906801

Gene position: 1267569-1267027 (Counterclockwise)

Preceding gene: 71906802

Following gene: 71906800

Centisome position: 28.16

GC content: 59.67

Gene sequence:

>543_bases
ATGATCAGCGCCCTAGCCATCATGGCCCTCGGGGCCATCATTCTCGGTGCCGCCCTTGGCTTTGCCTCGATCAAATTCAA
GGTCCATGGCAATCCGCTGGTCGAAAAAATCGAAGGCCTGCTGCCTCAGACCCAGTGCGGTCAATGCGGATTCCCCGGCT
GCCATCCCTACGCCGAAGCCATCGCCAAGGGCGAGGCCGACATCAATCTTTGCCCTCCCGGTGGCGAAGAAGGTGTCCGC
AAGCTGGCTGATCTGCTCGGGCGTGAAGTCAAGGCGCTGAACGCCGAAGAAAAACCAAAACAAGTGGCGGTCATCGACGA
AAACACCTGCATCGGCTGCACGCTGTGCATCCAGGCCTGCCCCGTCGATGCCATCGTCGGCGCGGCCAAGCAGCTGCACA
TCATCATCGCCCAGCAATGTACCGGCTGTGAACTCTGCCTGCCGCCCTGCCCGGTCGAATGCATTGCCATGGCCACCATC
CCGGAAACCGTGGATAACTGGAAATGGAAATACCCGGTTACCGAGATCAGGAAAGCTGCCTGA

Upstream 100 bases:

>100_bases
CCGACGTCCCGACCTATTTCAAGGGCACCGCCATCGCCCTCGTCACCGGCGGCTTGATGTCGCTGGCCTTCATGGGCTTC
GCCGGACTGGATAAATACCA

Downstream 100 bases:

>100_bases
TGCTGCAATTATTCAAGTTCAAGGGTGGCGTCAAACCAGCCACCAACAAGGTTCAGTCCGTCACCTTGCCGATTGCCACG
GCACCGCTGCCCTCCCGCCT

Product: electron transport complex protein RnfB

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MISALAIMALGAIILGAALGFASIKFKVHGNPLVEKIEGLLPQTQCGQCGFPGCHPYAEAIAKGEADINLCPPGGEEGVR
KLADLLGREVKALNAEEKPKQVAVIDENTCIGCTLCIQACPVDAIVGAAKQLHIIIAQQCTGCELCLPPCPVECIAMATI
PETVDNWKWKYPVTEIRKAA

Sequences:

>Translated_180_residues
MISALAIMALGAIILGAALGFASIKFKVHGNPLVEKIEGLLPQTQCGQCGFPGCHPYAEAIAKGEADINLCPPGGEEGVR
KLADLLGREVKALNAEEKPKQVAVIDENTCIGCTLCIQACPVDAIVGAAKQLHIIIAQQCTGCELCLPPCPVECIAMATI
PETVDNWKWKYPVTEIRKAA
>Mature_180_residues
MISALAIMALGAIILGAALGFASIKFKVHGNPLVEKIEGLLPQTQCGQCGFPGCHPYAEAIAKGEADINLCPPGGEEGVR
KLADLLGREVKALNAEEKPKQVAVIDENTCIGCTLCIQACPVDAIVGAAKQLHIIIAQQCTGCELCLPPCPVECIAMATI
PETVDNWKWKYPVTEIRKAA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=178, Percent_Identity=51.685393258427, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 18991; Mature: 18991

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISALAIMALGAIILGAALGFASIKFKVHGNPLVEKIEGLLPQTQCGQCGFPGCHPYAEA
CCHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
IAKGEADINLCPPGGEEGVRKLADLLGREVKALNAEEKPKQVAVIDENTCIGCTLCIQAC
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEHHHHHHHHC
PVDAIVGAAKQLHIIIAQQCTGCELCLPPCPVECIAMATIPETVDNWKWKYPVTEIRKAA
CHHHHHHHHHHEEEEEECCCCCCEEECCCCCHHHEEEHHCHHHHHCCEECCCHHHHHHCC
>Mature Secondary Structure
MISALAIMALGAIILGAALGFASIKFKVHGNPLVEKIEGLLPQTQCGQCGFPGCHPYAEA
CCHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
IAKGEADINLCPPGGEEGVRKLADLLGREVKALNAEEKPKQVAVIDENTCIGCTLCIQAC
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEHHHHHHHHC
PVDAIVGAAKQLHIIIAQQCTGCELCLPPCPVECIAMATIPETVDNWKWKYPVTEIRKAA
CHHHHHHHHHHEEEEEECCCCCCEEECCCCCHHHEEEHHCHHHHHCCEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA