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The map label for this gene is nth [H]

Identifier: 71906794

GI number: 71906794

Start: 1259690

End: 1260322

Strand: Reverse

Name: nth [H]

Synonym: Daro_1155

Alternate gene names: 71906794

Gene position: 1260322-1259690 (Counterclockwise)

Preceding gene: 71906796

Following gene: 71906793

Centisome position: 28.0

GC content: 59.87

Gene sequence:

>633_bases
GTGAAAAAAGCCAACATCGAACAGTTCTACAGCCGCCTGCGTGATGCCAACCCTGCACCGACGACAGAACTGCATTACGC
CACGCCTTTTCAGTTGCTGATTGCCGTCATTCTGTCGGCCCAGGCCACCGATGTCGGCGTCAACAAGGCGACGCTGCGGC
TGTTTCCGGTGGCGCCGACACCGGAAGCCATGCTGGCCCTGGGTGAAGAAGGACTGACGGAATACATCAAGACCATTGGC
CTGTTCCGGACCAAGGCAAAAAATGTCATGGCGACCTGCCGCATGCTCGTCGAGCAACATGGTGGAGAGGTACCCGATGA
TCGTGAGGCTCTCGAAGCCCTGCCCGGCGTCGGTCGGAAAACCGCCAATGTCGTCCTCAACACGGCTTTCGGCCACCCAA
CCATCGCCGTCGATACCCATATCTTCCGTCTCGGCAACCGCACCGGCCTGGCGCCGGGGAAGACCGTGCAGGAGGTCGAG
CAGAAGCTGCTGCGTGTCACGCCGGACGAATTCAAAAAGGATGCCCACCACTGGCTGATCCTGCATGGGCGCTACATTTG
CAAGGCCCGCAAACCGGAATGCAGCCGCTGCGTGGTCCTTGATCTCTGCAGCTTTCGCGGCAAGACGGTATGA

Upstream 100 bases:

>100_bases
CTGCGTCATAAGGTCCCCGCCTTCTCTGTCAGGTAGAATTTTGTTTTCTGCAATATCGCGTATTCAACGGCATCTGACAA
CCCATCTTTCTATCTACTCC

Downstream 100 bases:

>100_bases
AAGTGGCCAGCGGCCTGGTCACCGGGCACCGGCCTGTACCCGAGCTGGCCCGCGATGCCGTTCAGACCGCCTTGGAGGCA
GCCGGACTGCAACGGGCAGA

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKKANIEQFYSRLRDANPAPTTELHYATPFQLLIAVILSAQATDVGVNKATLRLFPVAPTPEAMLALGEEGLTEYIKTIG
LFRTKAKNVMATCRMLVEQHGGEVPDDREALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRLGNRTGLAPGKTVQEVE
QKLLRVTPDEFKKDAHHWLILHGRYICKARKPECSRCVVLDLCSFRGKTV

Sequences:

>Translated_210_residues
MKKANIEQFYSRLRDANPAPTTELHYATPFQLLIAVILSAQATDVGVNKATLRLFPVAPTPEAMLALGEEGLTEYIKTIG
LFRTKAKNVMATCRMLVEQHGGEVPDDREALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRLGNRTGLAPGKTVQEVE
QKLLRVTPDEFKKDAHHWLILHGRYICKARKPECSRCVVLDLCSFRGKTV
>Mature_210_residues
MKKANIEQFYSRLRDANPAPTTELHYATPFQLLIAVILSAQATDVGVNKATLRLFPVAPTPEAMLALGEEGLTEYIKTIG
LFRTKAKNVMATCRMLVEQHGGEVPDDREALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRLGNRTGLAPGKTVQEVE
QKLLRVTPDEFKKDAHHWLILHGRYICKARKPECSRCVVLDLCSFRGKTV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=68.2692307692308, Blast_Score=305, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17554540, Length=191, Percent_Identity=31.413612565445, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=160, Percent_Identity=32.5, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=33.1460674157303, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23243; Mature: 23243

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKANIEQFYSRLRDANPAPTTELHYATPFQLLIAVILSAQATDVGVNKATLRLFPVAPT
CCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
PEAMLALGEEGLTEYIKTIGLFRTKAKNVMATCRMLVEQHGGEVPDDREALEALPGVGRK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGHPTIAVDTHIFRLGNRTGLAPGKTVQEVEQKLLRVTPDEFKKDAHHWLI
HHHEEEECCCCCCEEEECHHHEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEE
LHGRYICKARKPECSRCVVLDLCSFRGKTV
EECEEEECCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKANIEQFYSRLRDANPAPTTELHYATPFQLLIAVILSAQATDVGVNKATLRLFPVAPT
CCCCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
PEAMLALGEEGLTEYIKTIGLFRTKAKNVMATCRMLVEQHGGEVPDDREALEALPGVGRK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGHPTIAVDTHIFRLGNRTGLAPGKTVQEVEQKLLRVTPDEFKKDAHHWLI
HHHEEEECCCCCCEEEECHHHEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEE
LHGRYICKARKPECSRCVVLDLCSFRGKTV
EECEEEECCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]