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The map label for this gene is ptsN [H]

Identifier: 71906722

GI number: 71906722

Start: 1187330

End: 1187896

Strand: Reverse

Name: ptsN [H]

Synonym: Daro_1083

Alternate gene names: 71906722

Gene position: 1187896-1187330 (Counterclockwise)

Preceding gene: 71906723

Following gene: 71906721

Centisome position: 26.39

GC content: 56.44

Gene sequence:

>567_bases
ATGCCAGCGCTTAAAACAGTGCGATGGGGAGCGGGCCGTTCCGCTCCCTTTTACCGCCAGTGGATCGAGCGATGGCGGGT
ACATCACCCAATCGAGGAATATCAATTGAATACCATCGCCGATCTACTCCGGGCAGAAGACATTCTTCTCAATCTCGACG
TTGCGACCAAAACCCAGCTTATTGAAGAAATCGGCCGGCACATGCAGGCGAGACATGGCATGCCGAAAGATTGGGTCATC
CTCAGCCTCTCCCGGCGGGAAGAAGTCGGCTCAACCGGCCTGGGCGAGGGAGTAGCCATTCCGCATGCCCGAATCAAGTA
TCTCGATCGCATTCAGATCGCCTATTTCCGCCTGGCATCACCGATTCCTTTTGCCGCCCCGGATGACAAACCGGTGTCCG
ACATCCTTGTCCTGCTCGTGCCGAAACAGGCGATGGAAGAACACCTCAACATTCTGGCCGATGCAACGCGGATGTTTTCC
AATGCCCGCTTTCGCGAGCGCCTGCACTTGTGTGAAGACGCATTGGCAGTACTTCAACTGTTCGCCAGCTGGCCGCACCT
GTCCTGA

Upstream 100 bases:

>100_bases
GACCTGAGCCCCGCAGGAGTTCAGACGGGTACGAAAACCAGAGATGCCGGTCGTGGCACCGCCGTTCTCTCCGTAACCAA
GAGTTTCTAAGTCAAGGCAG

Downstream 100 bases:

>100_bases
CTCGACAAGCTCAATTTATATGTTCTTTTTATAGCGAAGCACCAATATTTTTCAGAATCTTGAAATCGTTTTTCCTAGTA
TTCGTGATGTACCAGACCTG

Product: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MPALKTVRWGAGRSAPFYRQWIERWRVHHPIEEYQLNTIADLLRAEDILLNLDVATKTQLIEEIGRHMQARHGMPKDWVI
LSLSRREEVGSTGLGEGVAIPHARIKYLDRIQIAYFRLASPIPFAAPDDKPVSDILVLLVPKQAMEEHLNILADATRMFS
NARFRERLHLCEDALAVLQLFASWPHLS

Sequences:

>Translated_188_residues
MPALKTVRWGAGRSAPFYRQWIERWRVHHPIEEYQLNTIADLLRAEDILLNLDVATKTQLIEEIGRHMQARHGMPKDWVI
LSLSRREEVGSTGLGEGVAIPHARIKYLDRIQIAYFRLASPIPFAAPDDKPVSDILVLLVPKQAMEEHLNILADATRMFS
NARFRERLHLCEDALAVLQLFASWPHLS
>Mature_187_residues
PALKTVRWGAGRSAPFYRQWIERWRVHHPIEEYQLNTIADLLRAEDILLNLDVATKTQLIEEIGRHMQARHGMPKDWVIL
SLSRREEVGSTGLGEGVAIPHARIKYLDRIQIAYFRLASPIPFAAPDDKPVSDILVLLVPKQAMEEHLNILADATRMFSN
ARFRERLHLCEDALAVLQLFASWPHLS

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=114, Percent_Identity=30.7017543859649, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 21576; Mature: 21445

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALKTVRWGAGRSAPFYRQWIERWRVHHPIEEYQLNTIADLLRAEDILLNLDVATKTQL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
IEEIGRHMQARHGMPKDWVILSLSRREEVGSTGLGEGVAIPHARIKYLDRIQIAYFRLAS
HHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PIPFAAPDDKPVSDILVLLVPKQAMEEHLNILADATRMFSNARFRERLHLCEDALAVLQL
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FASWPHLS
HHCCCCCC
>Mature Secondary Structure 
PALKTVRWGAGRSAPFYRQWIERWRVHHPIEEYQLNTIADLLRAEDILLNLDVATKTQL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
IEEIGRHMQARHGMPKDWVILSLSRREEVGSTGLGEGVAIPHARIKYLDRIQIAYFRLAS
HHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PIPFAAPDDKPVSDILVLLVPKQAMEEHLNILADATRMFSNARFRERLHLCEDALAVLQL
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FASWPHLS
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]