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The map label for this gene is yhcA [H]

Identifier: 71906654

GI number: 71906654

Start: 1109150

End: 1110721

Strand: Reverse

Name: yhcA [H]

Synonym: Daro_1015

Alternate gene names: 71906654

Gene position: 1110721-1109150 (Counterclockwise)

Preceding gene: 71906656

Following gene: 71906652

Centisome position: 24.68

GC content: 60.62

Gene sequence:

>1572_bases
GTGGCGCACCTGCAAGGCCGGTTCAGTCGCCACCGCTCGCTCATGCCACAGCGCTCAGGCAAAATAACCAAAAAGGCACT
TGCCTCATCCGAGGCACCGTATACTTTTTTACCAGCCAACGAGCAGCCGACAATTCCGATGCGACGCCTCCGCCCAGCAA
CAAGCTTTGACACGCTTTCCCAACGTTATGGGGAACGCTACAAATGGATGGTGCTGTTCATTCTCGCGCTGGGTACCGTG
GCCGGGGTTCTTTGCACCTCCAGCTTCAATGTGGCCGTCCCGGCCCTCTCCCGGCACTTCCGGCTTGGGCAGGACCAAGT
GCAATGGGCCATGACCGGATTTCTGGCGGCGATGACCGTCGGTATGCTGCCGACCTCGTGGTTACTCGACCGCTTGGGCT
TTCGCATTATTTTCCTGCTTTCCCTGGCCGTCCTGACACTGGCCAGCGTGGGTGGTTACTTTGCTCCCAGCTTTACGCTC
GTCGTCGCGGCCCGCATTCTGCAGGGCCTGGCTGCCGGGGTGCTGCAACCGATGGGCGCATTGGCGCTGATGCGCCTCTT
TCCGCCGGAGATTCAGGGAAGAGCTTCCGGAATACTGATCTTCAGTATCGCCCTGACCCCGGCCATCGCGCCCTCGTTGG
GCGGCGCCTTGCTGGATCGCTTTGGCTGGGAGTCCATTTTTCTGCTGGGCACGCCATTCGCCGTCATTGCGGCGCTGTGC
GCCATTTATCTGTTACCGGCACCACGGGAGATCAAACGCAAGCCGTTCGACTGGCTGGGGCTGGGCTGGCTCACGCTGGG
CGCCATCGCCCTGATCGAAAGCGTATCGAGTCTTCAACACAGCGGCCTGGCCTCGCCGTGGACAATGGGCCAGTTTGCCC
TGGCTTTCGTCGCGTCGGCGCTGTATTACCGGCACGCCAGGCTGCGCAGCGACCCGCTGATCAACCTGGATTTGTTTCGG
CAACGCACCTTCGCCATGGGAACGCTGGTCTCCTTTGCCTATGGCTTTGGCCTGTACGCATCGACCTACCTGATTCCTGT
CTTTCTCCAGAATGCCTTGTCGTTCAAGGCGGGTGCCGCCGGCATGGCCTTGCTGCCTTCCGGGATCGCACTCGTCGTGA
TGCTGCCATTGGCCGGGCGCATGGCGGACCATTACTCGCCCAAATGGATCACCTTGATCGGACTCGTCGCCTTCGGTGCC
TCCTTCCTCATTTTTTCCATACGGGGCGGCGCCATTCAGTACGCCGAAATCATCGCGGCCACCATCATCGGCCGCATCGG
ACTCGGCATGATTCTGCCGGCACTCAACCTCGCGACGCTGCGTCACATGGAACCCCACCAACTGGGTCAATCCTCGGTCG
TGACCAGCTATGCCCGGCAATTGGGCGGTGTCATGGGAGTGGCGGTGCTGGCGGTCTTTGTCGAATGGCGCGAAACCGTT
TACGGCACAGTACCGCCGGGGATTTACTCGGCGTATTCCCAGGGCTTCCTGCTCCTCACCGCCGTTTTTTCGCTTGCCGT
GCTGGCAGCTTGTTTCATGAGGACAGAAGGTAGCCGTCCTTCCCCAAAATAA

Upstream 100 bases:

>100_bases
CTTGAAGGTGCTGGCAAGCCAGTCACCGAAAGGCGTAGTTTAGCTGCAACGGTGGTGAGAAAGCTGCCCCGACGGGCAGC
CTCGAAAACGGGGTAGCGGT

Downstream 100 bases:

>100_bases
CCGCTATCTGGAACACCCACGTTCAGACTGGGCGCGCGCTACCGGCCACTGGCAGTGTAGTCAGTCAATCCGTTGCGGCG
AGATTCACTTGCAACATGTT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MAHLQGRFSRHRSLMPQRSGKITKKALASSEAPYTFLPANEQPTIPMRRLRPATSFDTLSQRYGERYKWMVLFILALGTV
AGVLCTSSFNVAVPALSRHFRLGQDQVQWAMTGFLAAMTVGMLPTSWLLDRLGFRIIFLLSLAVLTLASVGGYFAPSFTL
VVAARILQGLAAGVLQPMGALALMRLFPPEIQGRASGILIFSIALTPAIAPSLGGALLDRFGWESIFLLGTPFAVIAALC
AIYLLPAPREIKRKPFDWLGLGWLTLGAIALIESVSSLQHSGLASPWTMGQFALAFVASALYYRHARLRSDPLINLDLFR
QRTFAMGTLVSFAYGFGLYASTYLIPVFLQNALSFKAGAAGMALLPSGIALVVMLPLAGRMADHYSPKWITLIGLVAFGA
SFLIFSIRGGAIQYAEIIAATIIGRIGLGMILPALNLATLRHMEPHQLGQSSVVTSYARQLGGVMGVAVLAVFVEWRETV
YGTVPPGIYSAYSQGFLLLTAVFSLAVLAACFMRTEGSRPSPK

Sequences:

>Translated_523_residues
MAHLQGRFSRHRSLMPQRSGKITKKALASSEAPYTFLPANEQPTIPMRRLRPATSFDTLSQRYGERYKWMVLFILALGTV
AGVLCTSSFNVAVPALSRHFRLGQDQVQWAMTGFLAAMTVGMLPTSWLLDRLGFRIIFLLSLAVLTLASVGGYFAPSFTL
VVAARILQGLAAGVLQPMGALALMRLFPPEIQGRASGILIFSIALTPAIAPSLGGALLDRFGWESIFLLGTPFAVIAALC
AIYLLPAPREIKRKPFDWLGLGWLTLGAIALIESVSSLQHSGLASPWTMGQFALAFVASALYYRHARLRSDPLINLDLFR
QRTFAMGTLVSFAYGFGLYASTYLIPVFLQNALSFKAGAAGMALLPSGIALVVMLPLAGRMADHYSPKWITLIGLVAFGA
SFLIFSIRGGAIQYAEIIAATIIGRIGLGMILPALNLATLRHMEPHQLGQSSVVTSYARQLGGVMGVAVLAVFVEWRETV
YGTVPPGIYSAYSQGFLLLTAVFSLAVLAACFMRTEGSRPSPK
>Mature_522_residues
AHLQGRFSRHRSLMPQRSGKITKKALASSEAPYTFLPANEQPTIPMRRLRPATSFDTLSQRYGERYKWMVLFILALGTVA
GVLCTSSFNVAVPALSRHFRLGQDQVQWAMTGFLAAMTVGMLPTSWLLDRLGFRIIFLLSLAVLTLASVGGYFAPSFTLV
VAARILQGLAAGVLQPMGALALMRLFPPEIQGRASGILIFSIALTPAIAPSLGGALLDRFGWESIFLLGTPFAVIAALCA
IYLLPAPREIKRKPFDWLGLGWLTLGAIALIESVSSLQHSGLASPWTMGQFALAFVASALYYRHARLRSDPLINLDLFRQ
RTFAMGTLVSFAYGFGLYASTYLIPVFLQNALSFKAGAAGMALLPSGIALVVMLPLAGRMADHYSPKWITLIGLVAFGAS
FLIFSIRGGAIQYAEIIAATIIGRIGLGMILPALNLATLRHMEPHQLGQSSVVTSYARQLGGVMGVAVLAVFVEWRETVY
GTVPPGIYSAYSQGFLLLTAVFSLAVLAACFMRTEGSRPSPK

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=416, Percent_Identity=25.2403846153846, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=27.7227722772277, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788710, Length=420, Percent_Identity=24.5238095238095, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=24.7619047619048, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788392, Length=307, Percent_Identity=30.2931596091205, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56501; Mature: 56370

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLQGRFSRHRSLMPQRSGKITKKALASSEAPYTFLPANEQPTIPMRRLRPATSFDTLS
CCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCHHHHH
QRYGERYKWMVLFILALGTVAGVLCTSSFNVAVPALSRHFRLGQDQVQWAMTGFLAAMTV
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GMLPTSWLLDRLGFRIIFLLSLAVLTLASVGGYFAPSFTLVVAARILQGLAAGVLQPMGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LALMRLFPPEIQGRASGILIFSIALTPAIAPSLGGALLDRFGWESIFLLGTPFAVIAALC
HHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
AIYLLPAPREIKRKPFDWLGLGWLTLGAIALIESVSSLQHSGLASPWTMGQFALAFVASA
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LYYRHARLRSDPLINLDLFRQRTFAMGTLVSFAYGFGLYASTYLIPVFLQNALSFKAGAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMALLPSGIALVVMLPLAGRMADHYSPKWITLIGLVAFGASFLIFSIRGGAIQYAEIIAA
CHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
TIIGRIGLGMILPALNLATLRHMEPHQLGQSSVVTSYARQLGGVMGVAVLAVFVEWRETV
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTVPPGIYSAYSQGFLLLTAVFSLAVLAACFMRTEGSRPSPK
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AHLQGRFSRHRSLMPQRSGKITKKALASSEAPYTFLPANEQPTIPMRRLRPATSFDTLS
CCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCHHHHH
QRYGERYKWMVLFILALGTVAGVLCTSSFNVAVPALSRHFRLGQDQVQWAMTGFLAAMTV
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GMLPTSWLLDRLGFRIIFLLSLAVLTLASVGGYFAPSFTLVVAARILQGLAAGVLQPMGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LALMRLFPPEIQGRASGILIFSIALTPAIAPSLGGALLDRFGWESIFLLGTPFAVIAALC
HHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
AIYLLPAPREIKRKPFDWLGLGWLTLGAIALIESVSSLQHSGLASPWTMGQFALAFVASA
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LYYRHARLRSDPLINLDLFRQRTFAMGTLVSFAYGFGLYASTYLIPVFLQNALSFKAGAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMALLPSGIALVVMLPLAGRMADHYSPKWITLIGLVAFGASFLIFSIRGGAIQYAEIIAA
CHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
TIIGRIGLGMILPALNLATLRHMEPHQLGQSSVVTSYARQLGGVMGVAVLAVFVEWRETV
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTVPPGIYSAYSQGFLLLTAVFSLAVLAACFMRTEGSRPSPK
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]