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The map label for this gene is pta [H]

Identifier: 71906614

GI number: 71906614

Start: 1066988

End: 1068406

Strand: Reverse

Name: pta [H]

Synonym: Daro_0975

Alternate gene names: 71906614

Gene position: 1068406-1066988 (Counterclockwise)

Preceding gene: 71906615

Following gene: 71906613

Centisome position: 23.74

GC content: 62.3

Gene sequence:

>1419_bases
ATGAACCACCAACCCGAACAACGCTATCTGGTTAACAAGACATTCGACGAAATCCGCATCGGCGACTCGGCCAGCCTCAA
GCGGACATTGATGCCCGAAGACGTCAAGCTGTTCGCGATCCTGACCGGCGACCTGAACCCGGCGGTCGTCGATCCGACGT
TTTCGGAAAGCGGCATGTTCCGCGAAGTCATCGCCCATGGCATGTGGAGTGGCTCGCTGATTTCAACCGTCCTCGGCACC
CAGTTTCCTGGCCCCGGCACCATCCTGGTCGACCAGACACTGCATTTCGCCCGCCCGGTCACCATCGGTGACACGATTAC
GATCACCGTCACCGCCAAACAGAAATTCGATCACAACAAACACGTCATCTTCGACTGCGTGTGCACCAACCAGGAAGGCC
TGCAGGTCGTGCGCGGCACCGCCGAAGTGCTGGCCCCGACCGAAAAGGTCTCCCGTCTGCAGGAAGTGCACATGCCGGAG
ATCCGCATCGACGACAAGCACGAGCGCTATATCCACCTGCTGGAACGCGTCAAAGGCCTGGAACCGATCCCCACTGCCGT
CGCTCATCCGTGCGATCAGGAATCGCTGAAAGGCCCGGTCATGGCCTTCCAGGCCGGCATCATCGAGCCCATTCTGGTCG
GCCCGGAGAACAAGATCCGCGCCGTCGCCGAGGAATTCGGCATCGACCTGCACGGCATCCGCATCGTCCATGCCGCACAC
AGCCATGATTCCGCCGCGCTGGCCGTCTCGCTGGTTCGCACCGGCGATGCCGAGGCGCTGATGAAGGGCAGCCTGCATAC
CGATGAGCTGATGGGCGAAGTCGTCGCCCGCGCCAACGGCCTGCGCACCAACCGCCGGATCAGCCACGTCTTCCTGATGG
ACGTGCCGACCTACCACCGCCCGCTGCTGATCACCGACGCCGCGATCAACATTGCGCCGACGCTGGAAGACAAGGTCGAC
ATCATCCAGAACGCCATCGACCTCGCCCACATCATCGGCATCCCGGAACCCAAGGTGGCCATCCTGTCGGCGGTCGAAAC
GGTCAACCCGAAGATTCAGTCCACGCTCGATGCCGCCGCCCTGTGCAAGATGGCCGACCGTGGCCAGATCAAGGGCGGCA
TCCTCGATGGCCCCCTCGCCTTCGACAACGCCGTCTCCATCGTCGCCGCCAAGACCAAGGGCATCAAGTCGGCCGTCGCC
GGCCATGCCGAAATCCTGGTGGTGCCCGATCTCGAATCCGGCAACATGATTGCCAAGCAGCTCGAATACCTGGCCAACGC
GCTGACCGCCGGCATCGTGCTCGGTACCAAGGTACCCATCGTGCTGACCAGCCGCGCCGACAGCGCCGAAACCCGTACTG
CATCGTGCGTCATTGCCGCACTGATCGCCCACCACAACCGCAAAACTGGAACTGTTTAA

Upstream 100 bases:

>100_bases
TGCCTCATCGTGGGAACACTACCGGCTACAGCCCAATATTGTTTGCTGCACCGCAGCAACCTATACTAGAACATCGCGTG
ACTCACTGGAGCAGGGATTC

Downstream 100 bases:

>100_bases
ATGAAGCAAGGCATCCTGACGATCAATGCTGGATCGTCGTCCATCAAGTTCGCCCTCTTCCCACTCGACCATCCGATTTC
CCCGGAAGCTGAAGTCTCCG

Product: bifunctional enoyl-CoA hydratase/phosphate acetyltransferase

Products: NA

Alternate protein names: Phosphotransacetylase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNHQPEQRYLVNKTFDEIRIGDSASLKRTLMPEDVKLFAILTGDLNPAVVDPTFSESGMFREVIAHGMWSGSLISTVLGT
QFPGPGTILVDQTLHFARPVTIGDTITITVTAKQKFDHNKHVIFDCVCTNQEGLQVVRGTAEVLAPTEKVSRLQEVHMPE
IRIDDKHERYIHLLERVKGLEPIPTAVAHPCDQESLKGPVMAFQAGIIEPILVGPENKIRAVAEEFGIDLHGIRIVHAAH
SHDSAALAVSLVRTGDAEALMKGSLHTDELMGEVVARANGLRTNRRISHVFLMDVPTYHRPLLITDAAINIAPTLEDKVD
IIQNAIDLAHIIGIPEPKVAILSAVETVNPKIQSTLDAAALCKMADRGQIKGGILDGPLAFDNAVSIVAAKTKGIKSAVA
GHAEILVVPDLESGNMIAKQLEYLANALTAGIVLGTKVPIVLTSRADSAETRTASCVIAALIAHHNRKTGTV

Sequences:

>Translated_472_residues
MNHQPEQRYLVNKTFDEIRIGDSASLKRTLMPEDVKLFAILTGDLNPAVVDPTFSESGMFREVIAHGMWSGSLISTVLGT
QFPGPGTILVDQTLHFARPVTIGDTITITVTAKQKFDHNKHVIFDCVCTNQEGLQVVRGTAEVLAPTEKVSRLQEVHMPE
IRIDDKHERYIHLLERVKGLEPIPTAVAHPCDQESLKGPVMAFQAGIIEPILVGPENKIRAVAEEFGIDLHGIRIVHAAH
SHDSAALAVSLVRTGDAEALMKGSLHTDELMGEVVARANGLRTNRRISHVFLMDVPTYHRPLLITDAAINIAPTLEDKVD
IIQNAIDLAHIIGIPEPKVAILSAVETVNPKIQSTLDAAALCKMADRGQIKGGILDGPLAFDNAVSIVAAKTKGIKSAVA
GHAEILVVPDLESGNMIAKQLEYLANALTAGIVLGTKVPIVLTSRADSAETRTASCVIAALIAHHNRKTGTV
>Mature_472_residues
MNHQPEQRYLVNKTFDEIRIGDSASLKRTLMPEDVKLFAILTGDLNPAVVDPTFSESGMFREVIAHGMWSGSLISTVLGT
QFPGPGTILVDQTLHFARPVTIGDTITITVTAKQKFDHNKHVIFDCVCTNQEGLQVVRGTAEVLAPTEKVSRLQEVHMPE
IRIDDKHERYIHLLERVKGLEPIPTAVAHPCDQESLKGPVMAFQAGIIEPILVGPENKIRAVAEEFGIDLHGIRIVHAAH
SHDSAALAVSLVRTGDAEALMKGSLHTDELMGEVVARANGLRTNRRISHVFLMDVPTYHRPLLITDAAINIAPTLEDKVD
IIQNAIDLAHIIGIPEPKVAILSAVETVNPKIQSTLDAAALCKMADRGQIKGGILDGPLAFDNAVSIVAAKTKGIKSAVA
GHAEILVVPDLESGNMIAKQLEYLANALTAGIVLGTKVPIVLTSRADSAETRTASCVIAALIAHHNRKTGTV

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=292, Percent_Identity=26.3698630136986, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788800, Length=325, Percent_Identity=25.2307692307692, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 50866; Mature: 50866

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQPEQRYLVNKTFDEIRIGDSASLKRTLMPEDVKLFAILTGDLNPAVVDPTFSESGMF
CCCCCCHHHHHHCCHHHEECCCCCCHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCHH
REVIAHGMWSGSLISTVLGTQFPGPGTILVDQTLHFARPVTIGDTITITVTAKQKFDHNK
HHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCHHHHCCEEECCEEEEEEEEHHCCCCCC
HVIFDCVCTNQEGLQVVRGTAEVLAPTEKVSRLQEVHMPEIRIDDKHERYIHLLERVKGL
CEEEEEEECCCHHHHHHHCHHHHHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCC
EPIPTAVAHPCDQESLKGPVMAFQAGIIEPILVGPENKIRAVAEEFGIDLHGIRIVHAAH
CCCCHHHCCCCCHHHCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEEEEEC
SHDSAALAVSLVRTGDAEALMKGSLHTDELMGEVVARANGLRTNRRISHVFLMDVPTYHR
CCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
PLLITDAAINIAPTLEDKVDIIQNAIDLAHIIGIPEPKVAILSAVETVNPKIQSTLDAAA
CEEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LCKMADRGQIKGGILDGPLAFDNAVSIVAAKTKGIKSAVAGHAEILVVPDLESGNMIAKQ
HHHHHCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHHHCCCEEEEEEECCCCCCHHHHH
LEYLANALTAGIVLGTKVPIVLTSRADSAETRTASCVIAALIAHHNRKTGTV
HHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNHQPEQRYLVNKTFDEIRIGDSASLKRTLMPEDVKLFAILTGDLNPAVVDPTFSESGMF
CCCCCCHHHHHHCCHHHEECCCCCCHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCHH
REVIAHGMWSGSLISTVLGTQFPGPGTILVDQTLHFARPVTIGDTITITVTAKQKFDHNK
HHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCHHHHCCEEECCEEEEEEEEHHCCCCCC
HVIFDCVCTNQEGLQVVRGTAEVLAPTEKVSRLQEVHMPEIRIDDKHERYIHLLERVKGL
CEEEEEEECCCHHHHHHHCHHHHHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCC
EPIPTAVAHPCDQESLKGPVMAFQAGIIEPILVGPENKIRAVAEEFGIDLHGIRIVHAAH
CCCCHHHCCCCCHHHCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEEEEEC
SHDSAALAVSLVRTGDAEALMKGSLHTDELMGEVVARANGLRTNRRISHVFLMDVPTYHR
CCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
PLLITDAAINIAPTLEDKVDIIQNAIDLAHIIGIPEPKVAILSAVETVNPKIQSTLDAAA
CEEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LCKMADRGQIKGGILDGPLAFDNAVSIVAAKTKGIKSAVAGHAEILVVPDLESGNMIAKQ
HHHHHCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHHHCCCEEEEEEECCCCCCHHHHH
LEYLANALTAGIVLGTKVPIVLTSRADSAETRTASCVIAALIAHHNRKTGTV
HHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]