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The map label for this gene is pckG [H]

Identifier: 71906304

GI number: 71906304

Start: 731283

End: 733127

Strand: Reverse

Name: pckG [H]

Synonym: Daro_0665

Alternate gene names: 71906304

Gene position: 733127-731283 (Counterclockwise)

Preceding gene: 71906307

Following gene: 71906303

Centisome position: 16.29

GC content: 61.3

Gene sequence:

>1845_bases
ATGACCGCACCGCTGAATGCACCTGACTACGTCAAACATGAAGGCCTGAAAAAATGGGTCGCCGAGATCGCCGCGCTGAC
CCAGCCGGACCGTATCCACTGGGCCGACGGGTCGCAGGAAGAGTATGACCGCCTGTGCGCAGAAATGGTAGAGGCCGGCA
CGCTGATCAAGCTGAACGAAGCCAAGCGTCCTAATTCCTACCTCGCCTTCTCCGACCCGTCCGATGTGGCGCGTGTCGAA
GACCGCACCTACATCTGCTCCGCCAACAAGGAAGACGCCGGCCCGACCAATAACTGGGAAGCACCGGCCGTCATGCGCGA
AAAGCTGAACGGCATCTTCAAGGGCTGCATGAAGGGCCGTACGATGTACGTGATCCCGTTCTCGATGGGCCCGCTCGGTT
CGCCGATCGCCCACATCGGTTTCGAGATTACCGACTCCGCCTACGTCGTGACCAACATGCGCACGATGACCCGCATGGGC
AAGGCCGTTTTCGACGTGCTGGGCGCCGATGGCGAATTCGTGCCCTGCATCCACACGGTCGGCGCGCCGCTTGAACCGGG
TCAGCAGGATGCCAAGTGGCCGTGCAACCCGACCGTCAAGTACATCGTGCACTACCCGGAAACCCGCGAAATCTGGTCCT
ACGGCTCCGGTTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCCAACATGGCGCGCGAT
CAGGGCTGGCTGGCCGAACACATGCTGATTCTCGGCGTCGAATCGCCGGAAGGTGAAAAGTCCTACGTCGCTGCCGCCTT
CCCGTCGGCCTGCGGCAAGACCAACTTCGCCATGCTGATCCCGCCGAAGACCCTCGACGGCTGGAAGATCACCACCATCG
GTGACGACATCGCCTGGATCAAGCCGCGTGTCGACAAGGACGGTGTCACCCGCTTCTACGCCATCAACCCGGAAGCCGGT
TTCTTCGGCGTCGCCCCGGGCACCTCGGAAAAGACCAACTTCAACGCCTTGGCCACGCTCAAGGAAAACATCATCTTCAC
CAACGTCGCGCTGACCGACGACGGCGACGTGTGGTGGGAAGGCCTGACCAAGGAAGCTCCGGCGCACCTGATCGACTGGC
AAGGCAACGACTGGACCCCGGAAGATGGCAAGGCCGGCAAGAAGGCCGCCCACGCCAACTCCCGCTTCACCGCCCCGGCC
AGCCAGTGCCCGTCCGTTGATTCCGCTTGGGAAGACCCGGCCGGTGTGCCGATTTCCGCCTTTATCTTCGGCGGCCGTCG
CGCCACCACCGTGCCGCTGGTCTATGAAGCCTTCAACTGGAACTACGGCGTCTACATGGCGGCCACCCTGGGCTCCGAAA
CCACCGCTGCCGCCTTTGGCCAGCAAGGTGTTGTCCGCCGCGACCCGTTCGCCATGCTGCCGTTCTGCGGCTACCACATG
GGCGATTACTTCAACCACTGGTTGAAGATGGGCAAGACCGTTGATGCCGCCCCGAAGATCTTCTGCGTGAACTGGTTCCG
CATGAACGAAAAGGGCGAATTCATGTGGCCGGGCTTCGGTGACAACATGCGCGTCCTGAAGTGGATCGTTCAGCGCTGCA
AGGGCAAGGTTTCTGCCAAGGAAACCGCGCTGGGCTGGATGCCGCAGTTTGCTGACATCGACTGGACCGGCCTCGACATC
AGCAAGGCTGAATTCGACGGTCTGACCAAAATCGATGCCGACGCCTGGAAATCCGAACTGGCCGGCCACAAGGAGTGGTT
CGACAAGATGGGCGAAAAGTTGCCACGCGAATTCACGCTGAAACGCGAACTATTCGAAATGGCACTGTTCAAGAACGCTG
ACTAA

Upstream 100 bases:

>100_bases
TTCCCAGCCGACGGCCCCTAGCCGCTTTCAGTCTTGGTGGCAGCTCCCGGCGACTGGCGCAAAGTTTATCTATTTCAGTA
ACTTGGAGAGAGACATCGCC

Downstream 100 bases:

>100_bases
TTCCAGTTCTTGCTATGATCGACGGCCACCTCAGGGTGGCCGTTTTACTTTGGAGAACCGCATGTACCGTCCCGCCCGAC
GCCTCGCCGATATCGAGCCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MTAPLNAPDYVKHEGLKKWVAEIAALTQPDRIHWADGSQEEYDRLCAEMVEAGTLIKLNEAKRPNSYLAFSDPSDVARVE
DRTYICSANKEDAGPTNNWEAPAVMREKLNGIFKGCMKGRTMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMRTMTRMG
KAVFDVLGADGEFVPCIHTVGAPLEPGQQDAKWPCNPTVKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMARD
QGWLAEHMLILGVESPEGEKSYVAAAFPSACGKTNFAMLIPPKTLDGWKITTIGDDIAWIKPRVDKDGVTRFYAINPEAG
FFGVAPGTSEKTNFNALATLKENIIFTNVALTDDGDVWWEGLTKEAPAHLIDWQGNDWTPEDGKAGKKAAHANSRFTAPA
SQCPSVDSAWEDPAGVPISAFIFGGRRATTVPLVYEAFNWNYGVYMAATLGSETTAAAFGQQGVVRRDPFAMLPFCGYHM
GDYFNHWLKMGKTVDAAPKIFCVNWFRMNEKGEFMWPGFGDNMRVLKWIVQRCKGKVSAKETALGWMPQFADIDWTGLDI
SKAEFDGLTKIDADAWKSELAGHKEWFDKMGEKLPREFTLKRELFEMALFKNAD

Sequences:

>Translated_614_residues
MTAPLNAPDYVKHEGLKKWVAEIAALTQPDRIHWADGSQEEYDRLCAEMVEAGTLIKLNEAKRPNSYLAFSDPSDVARVE
DRTYICSANKEDAGPTNNWEAPAVMREKLNGIFKGCMKGRTMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMRTMTRMG
KAVFDVLGADGEFVPCIHTVGAPLEPGQQDAKWPCNPTVKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMARD
QGWLAEHMLILGVESPEGEKSYVAAAFPSACGKTNFAMLIPPKTLDGWKITTIGDDIAWIKPRVDKDGVTRFYAINPEAG
FFGVAPGTSEKTNFNALATLKENIIFTNVALTDDGDVWWEGLTKEAPAHLIDWQGNDWTPEDGKAGKKAAHANSRFTAPA
SQCPSVDSAWEDPAGVPISAFIFGGRRATTVPLVYEAFNWNYGVYMAATLGSETTAAAFGQQGVVRRDPFAMLPFCGYHM
GDYFNHWLKMGKTVDAAPKIFCVNWFRMNEKGEFMWPGFGDNMRVLKWIVQRCKGKVSAKETALGWMPQFADIDWTGLDI
SKAEFDGLTKIDADAWKSELAGHKEWFDKMGEKLPREFTLKRELFEMALFKNAD
>Mature_613_residues
TAPLNAPDYVKHEGLKKWVAEIAALTQPDRIHWADGSQEEYDRLCAEMVEAGTLIKLNEAKRPNSYLAFSDPSDVARVED
RTYICSANKEDAGPTNNWEAPAVMREKLNGIFKGCMKGRTMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMRTMTRMGK
AVFDVLGADGEFVPCIHTVGAPLEPGQQDAKWPCNPTVKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMARDQ
GWLAEHMLILGVESPEGEKSYVAAAFPSACGKTNFAMLIPPKTLDGWKITTIGDDIAWIKPRVDKDGVTRFYAINPEAGF
FGVAPGTSEKTNFNALATLKENIIFTNVALTDDGDVWWEGLTKEAPAHLIDWQGNDWTPEDGKAGKKAAHANSRFTAPAS
QCPSVDSAWEDPAGVPISAFIFGGRRATTVPLVYEAFNWNYGVYMAATLGSETTAAAFGQQGVVRRDPFAMLPFCGYHMG
DYFNHWLKMGKTVDAAPKIFCVNWFRMNEKGEFMWPGFGDNMRVLKWIVQRCKGKVSAKETALGWMPQFADIDWTGLDIS
KAEFDGLTKIDADAWKSELAGHKEWFDKMGEKLPREFTLKRELFEMALFKNAD

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=611, Percent_Identity=50.5728314238953, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI187281517, Length=609, Percent_Identity=50.9031198686371, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI66346723, Length=378, Percent_Identity=53.7037037037037, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17555492, Length=607, Percent_Identity=49.9176276771005, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989645, Length=596, Percent_Identity=50.1677852348993, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989658, Length=596, Percent_Identity=50.1677852348993, Blast_Score=556, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=562, Percent_Identity=51.779359430605, Blast_Score=551, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71989653, Length=584, Percent_Identity=50.513698630137, Blast_Score=547, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=506, Percent_Identity=53.1620553359684, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=489, Percent_Identity=52.9652351738241, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564409, Length=361, Percent_Identity=49.584487534626, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17540956, Length=516, Percent_Identity=25.1937984496124, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24655085, Length=606, Percent_Identity=50.4950495049505, Blast_Score=590, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=593, Percent_Identity=50.2529510961214, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI161077226, Length=560, Percent_Identity=51.25, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24655088, Length=499, Percent_Identity=53.1062124248497, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68137; Mature: 68006

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLNAPDYVKHEGLKKWVAEIAALTQPDRIHWADGSQEEYDRLCAEMVEAGTLIKLNE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECC
AKRPNSYLAFSDPSDVARVEDRTYICSANKEDAGPTNNWEAPAVMREKLNGIFKGCMKGR
CCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMRTMTRMGKAVFDVLGADGEFVPCIHTV
EEEEEEECCCCCCCCHHHCCEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCEECHHHHC
GAPLEPGQQDAKWPCNPTVKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMARD
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QGWLAEHMLILGVESPEGEKSYVAAAFPSACGKTNFAMLIPPKTLDGWKITTIGDDIAWI
CCHHHEEEEEEEECCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPRVDKDGVTRFYAINPEAGFFGVAPGTSEKTNFNALATLKENIIFTNVALTDDGDVWWE
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCHHHC
GLTKEAPAHLIDWQGNDWTPEDGKAGKKAAHANSRFTAPASQCPSVDSAWEDPAGVPISA
CCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEE
FIFGGRRATTVPLVYEAFNWNYGVYMAATLGSETTAAAFGQQGVVRRDPFAMLPFCGYHM
EEECCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHH
GDYFNHWLKMGKTVDAAPKIFCVNWFRMNEKGEFMWPGFGDNMRVLKWIVQRCKGKVSAK
HHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCH
ETALGWMPQFADIDWTGLDISKAEFDGLTKIDADAWKSELAGHKEWFDKMGEKLPREFTL
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHH
KRELFEMALFKNAD
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TAPLNAPDYVKHEGLKKWVAEIAALTQPDRIHWADGSQEEYDRLCAEMVEAGTLIKLNE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECC
AKRPNSYLAFSDPSDVARVEDRTYICSANKEDAGPTNNWEAPAVMREKLNGIFKGCMKGR
CCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMRTMTRMGKAVFDVLGADGEFVPCIHTV
EEEEEEECCCCCCCCHHHCCEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCEECHHHHC
GAPLEPGQQDAKWPCNPTVKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIASNMARD
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QGWLAEHMLILGVESPEGEKSYVAAAFPSACGKTNFAMLIPPKTLDGWKITTIGDDIAWI
CCHHHEEEEEEEECCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPRVDKDGVTRFYAINPEAGFFGVAPGTSEKTNFNALATLKENIIFTNVALTDDGDVWWE
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCHHHC
GLTKEAPAHLIDWQGNDWTPEDGKAGKKAAHANSRFTAPASQCPSVDSAWEDPAGVPISA
CCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEE
FIFGGRRATTVPLVYEAFNWNYGVYMAATLGSETTAAAFGQQGVVRRDPFAMLPFCGYHM
EEECCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHH
GDYFNHWLKMGKTVDAAPKIFCVNWFRMNEKGEFMWPGFGDNMRVLKWIVQRCKGKVSAK
HHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCH
ETALGWMPQFADIDWTGLDISKAEFDGLTKIDADAWKSELAGHKEWFDKMGEKLPREFTL
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHH
KRELFEMALFKNAD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA