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The map label for this gene is folA [H]

Identifier: 71906219

GI number: 71906219

Start: 646778

End: 647269

Strand: Reverse

Name: folA [H]

Synonym: Daro_0579

Alternate gene names: 71906219

Gene position: 647269-646778 (Counterclockwise)

Preceding gene: 71906220

Following gene: 71906214

Centisome position: 14.38

GC content: 60.77

Gene sequence:

>492_bases
ATGCCCGAAATCATTCTCATCGCCGCCGTCGCCAAAAACCGGGTGATCGGCAAGGACAACACGCTGATCTGGAACCTTCC
AGAGGACATGGCGCACTTCAAGGCGCTGACCGCCGGCCAGACCGTGGTCATGGGTCGCAAGACCTGGGAGTCGCTGCCGC
CACGTTTCCGCCCCCTACCGGGTCGCCGCAACGTCGTGATCAGCCGCCAGGCCGATTACGCCGCCCCCGGCGCCGAAGTT
GCCAACTCGCTGGAAAACGCCCTGCAACTGGCGTCGACGGCTGAAAGCGTTTTTATCATTGGCGGCGAGCAGATTTACAA
ACAGGCGATGGCAGTCGCCGACCGCCTTGAAATCACCGACGTCGACCTCGAACCGGCAGGCGATGCCTGGTTCCCGGAAA
TCGCAGCAACCGACTGGTCAGTCACACAAAAAAACACCCCGCCCAGCCAAAGCGGAACGGGGTTTTCTTTTGTGACCTAT
CGCCGCAAATAG

Upstream 100 bases:

>100_bases
AGCAGAACGGCATGCGCCGGGATTTTTCCTGGACGCACGCCGCCAACGAATACGTTGCGCTCTATCGCGACGCCATCGCT
ACCCGAGCTTAAGCACCAGC

Downstream 100 bases:

>100_bases
CGGCCGGGTTCAGCGCACGCAATCGACGTAGTAGCTGCCGTCCCCGCCCTTGACCAGACCGTGCACGTCGGTCTCGAAGC
CGGGGAAGCGGGCGTTGAAC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MPEIILIAAVAKNRVIGKDNTLIWNLPEDMAHFKALTAGQTVVMGRKTWESLPPRFRPLPGRRNVVISRQADYAAPGAEV
ANSLENALQLASTAESVFIIGGEQIYKQAMAVADRLEITDVDLEPAGDAWFPEIAATDWSVTQKNTPPSQSGTGFSFVTY
RRK

Sequences:

>Translated_163_residues
MPEIILIAAVAKNRVIGKDNTLIWNLPEDMAHFKALTAGQTVVMGRKTWESLPPRFRPLPGRRNVVISRQADYAAPGAEV
ANSLENALQLASTAESVFIIGGEQIYKQAMAVADRLEITDVDLEPAGDAWFPEIAATDWSVTQKNTPPSQSGTGFSFVTY
RRK
>Mature_162_residues
PEIILIAAVAKNRVIGKDNTLIWNLPEDMAHFKALTAGQTVVMGRKTWESLPPRFRPLPGRRNVVISRQADYAAPGAEVA
NSLENALQLASTAESVFIIGGEQIYKQAMAVADRLEITDVDLEPAGDAWFPEIAATDWSVTQKNTPPSQSGTGFSFVTYR
RK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=152, Percent_Identity=34.2105263157895, Blast_Score=81, Evalue=5e-16,
Organism=Homo sapiens, GI28827793, Length=152, Percent_Identity=32.8947368421053, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786233, Length=166, Percent_Identity=36.144578313253, Blast_Score=109, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17505899, Length=190, Percent_Identity=30.5263157894737, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=139, Percent_Identity=33.8129496402878, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI116008026, Length=143, Percent_Identity=33.5664335664336, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24647458, Length=143, Percent_Identity=33.5664335664336, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17871; Mature: 17739

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIILIAAVAKNRVIGKDNTLIWNLPEDMAHFKALTAGQTVVMGRKTWESLPPRFRPLP
CCCEEEEEEECCCCEECCCCEEEEECCHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCC
GRRNVVISRQADYAAPGAEVANSLENALQLASTAESVFIIGGEQIYKQAMAVADRLEITD
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCEEEE
VDLEPAGDAWFPEIAATDWSVTQKNTPPSQSGTGFSFVTYRRK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
PEIILIAAVAKNRVIGKDNTLIWNLPEDMAHFKALTAGQTVVMGRKTWESLPPRFRPLP
CCEEEEEEECCCCEECCCCEEEEECCHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCC
GRRNVVISRQADYAAPGAEVANSLENALQLASTAESVFIIGGEQIYKQAMAVADRLEITD
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCEEEE
VDLEPAGDAWFPEIAATDWSVTQKNTPPSQSGTGFSFVTYRRK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]