Click here to switch to the map view.

The map label for this gene is metG

Identifier: 71906214

GI number: 71906214

Start: 639692

End: 641782

Strand: Reverse

Name: metG

Synonym: Daro_0574

Alternate gene names: 71906214

Gene position: 641782-639692 (Counterclockwise)

Preceding gene: 71906219

Following gene: 71906213

Centisome position: 14.26

GC content: 60.07

Gene sequence:

>2091_bases
ATGTCGCGCAAAATCCTTGTCACTTCCGCTCTGCCCTACGCCAATGGCGCCATTCACCTCGGCCATCTGGTCGAATATAT
CCAGACCGACATCTGGGTACGATTCCAGAAGATGTCCGGCAACGAATGCTGGTACGTCTGCGCCGATGACACCCATGGCA
CCCCGATCATGCTGCGCGCGGAAAAGGAAGGCATCACCCCGGAACAACTGATCGCCCGCGTGCATGGCGAGCATTCCCGC
GATTTCGCCGGTTTCCATGTCGGTTTCGACAATTACTACAGCACACACTCCGACGAAACCCGCGACTGCGCCAATGACAT
CTATCTGAAGCTGCGCGCTGCCGGCCTGATCGAAACGCGGACCATTGAGCAGTATTACGACCCGGTCAAACAACTCTTCC
TGCCGGACCGCTTCATCAAGGGCGAGTGCCCGAAGTGCGGCGCCAAGGACCAGTATGGCGACAACTGCGAGTCCTGCGGT
GCCGCCTACGCGCCGACCGACCTCAAGGAACCGTACTCCGCCATTTCCGGCGCCAAGCCGGAACTGCGTACCTCTGAACA
CTATTTCTTCAAGCTGTCAGACCCGCGCTGCGAAGCCTTCCTGCGCCAGTACACCAGTCGCGACAACGGCGTGCTGCAGA
ACGAAGCGGCCAACAAGATGCAGGAATGGCTGGGCGCTCCCGGCGAGAACAAGCTGACCGACTGGGACATTTCACGCGAC
GCGCCCTATTTCGGCTTCGAGATTCCCGATGCGCCGGGCAAATACTTCTATGTCTGGCTCGATGCACCGATCGGCTACAT
GGGCTCGTTCAAGAACCTGTGCGCGCGCAACGGCCTCGACTTCAACGAATACTTCAAACCCGACGCCAAGACCGAGCTCT
ACCATTTCATCGGCAAGGACATTCTTTACTTCCACGCCCTGTTCTGGCCGGCCGAACTGGCCCATGCCGGCTTCCGCACG
CCGACCAAGATCTTCGCGCACGGTTTCCTGACCGTCGATGGGGCCAAGATGTCCAAATCGCGCGGCACCTTCATCACCGC
TGAGAGCTTCCTGAAGACCGGGCTGAACCCTGAGTGGCTACGTTATTACTATGCGGCCAAGCTGTCGGCGACGATGGAAG
ACATCGACCTCAGCCTCGATGACTTCGTCGCCCGCGTCAATTCCGACCTCGTCGGCAAGTACGTCAATATTGCCAGCCGT
TCGGCCGGCTTCATCACCAAGCGCTTCAACGGCCAACTGGCCCCGGCCACTGCCGAGCTGCCGGCCATCAAGGGCATCCA
GGAAGCCGCCAGTCGCATCGCCGAGCTTTATGAAGCCCGCGAGTTCGGCAAGGCCATGCGCGAAATCATGGCGCTGACCG
ATGCCGCCAACCAGTACGTCGACAGCGTCAAGCCCTGGGAGCTGGCCAAGCAGGAAGGCAAGGAAGTCGAACTGCACGCT
GCCTGCACCAATGCGCTGAACCTGTTCCGCCTGCTGACCGTGCTGCTGAAGCCGATCCTGCCTATCGTTGCCGAGAAGGT
CGAGAAATTCCTCAACATCGCCCCGCTGCACTGGGCCGACACGCAAAGCCTGCTCGCCGGCGGCCATGCGATCAATGCTT
ACGAGCACCTGATGACCCGCGTCGATCCCAAGCTGATCGAAAAGCTGGTCGAGGCCAACAAGGAATCGCTGGCTCCGGCG
CCGGAAGCCCAGTCCCAGCAGCGCCATGCCGAACACCAGCAAAACGAGGTGACGGCCGAAAGCCCGTGGGAACCGTTCTG
CAATATTGACGACTTCATGAAGGTCGATCTGCGCATTGTTCGCATCGCCAACGCCGAACACGTCGAAGGCGCCGACAAGC
TGGTGCGCCTGACGCTGGATGTCGGCAACAACGAAACCCGCAATGTCTTCGCCGGCATCAAGGCGGCTTACGACCCAGCG
CAGTTGATCGGGCGACTGACCGTCATGGTCGCCAATCTGGCACCGCGAAAAATGAAATTTGGCCTGTCGGAAGGAATGGT
TCTCGCGGCCTCTGATGGCGAAGGAAAAACCCCGGGCATCTTCCTGCTATCGCCGGATTCCGGCGCTCAGCCAGGCATGC
GCGTCAAGTAA

Upstream 100 bases:

>100_bases
CGTAACAAATTCAGGCGATGCTAATGAATCTCCGTTTGCAACTCATCACCATGATTCCTCGCGGTAAAATCGCGCTTTAC
CTTTAAAGCACCGGCCTCTC

Downstream 100 bases:

>100_bases
ATTGATGCCAATGGAAACGGCCCGCTTAGCGGGCCGTTTCCTTGCTGCGGCGCAACAAAAGCGCCCATCAACTTCAAGTA
TCACTTTAACTTAGCACCCC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECWYVCADDTHGTPIMLRAEKEGITPEQLIARVHGEHSR
DFAGFHVGFDNYYSTHSDETRDCANDIYLKLRAAGLIETRTIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCG
AAYAPTDLKEPYSAISGAKPELRTSEHYFFKLSDPRCEAFLRQYTSRDNGVLQNEAANKMQEWLGAPGENKLTDWDISRD
APYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCARNGLDFNEYFKPDAKTELYHFIGKDILYFHALFWPAELAHAGFRT
PTKIFAHGFLTVDGAKMSKSRGTFITAESFLKTGLNPEWLRYYYAAKLSATMEDIDLSLDDFVARVNSDLVGKYVNIASR
SAGFITKRFNGQLAPATAELPAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHA
ACTNALNLFRLLTVLLKPILPIVAEKVEKFLNIAPLHWADTQSLLAGGHAINAYEHLMTRVDPKLIEKLVEANKESLAPA
PEAQSQQRHAEHQQNEVTAESPWEPFCNIDDFMKVDLRIVRIANAEHVEGADKLVRLTLDVGNNETRNVFAGIKAAYDPA
QLIGRLTVMVANLAPRKMKFGLSEGMVLAASDGEGKTPGIFLLSPDSGAQPGMRVK

Sequences:

>Translated_696_residues
MSRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECWYVCADDTHGTPIMLRAEKEGITPEQLIARVHGEHSR
DFAGFHVGFDNYYSTHSDETRDCANDIYLKLRAAGLIETRTIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCG
AAYAPTDLKEPYSAISGAKPELRTSEHYFFKLSDPRCEAFLRQYTSRDNGVLQNEAANKMQEWLGAPGENKLTDWDISRD
APYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCARNGLDFNEYFKPDAKTELYHFIGKDILYFHALFWPAELAHAGFRT
PTKIFAHGFLTVDGAKMSKSRGTFITAESFLKTGLNPEWLRYYYAAKLSATMEDIDLSLDDFVARVNSDLVGKYVNIASR
SAGFITKRFNGQLAPATAELPAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHA
ACTNALNLFRLLTVLLKPILPIVAEKVEKFLNIAPLHWADTQSLLAGGHAINAYEHLMTRVDPKLIEKLVEANKESLAPA
PEAQSQQRHAEHQQNEVTAESPWEPFCNIDDFMKVDLRIVRIANAEHVEGADKLVRLTLDVGNNETRNVFAGIKAAYDPA
QLIGRLTVMVANLAPRKMKFGLSEGMVLAASDGEGKTPGIFLLSPDSGAQPGMRVK
>Mature_695_residues
SRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECWYVCADDTHGTPIMLRAEKEGITPEQLIARVHGEHSRD
FAGFHVGFDNYYSTHSDETRDCANDIYLKLRAAGLIETRTIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCGA
AYAPTDLKEPYSAISGAKPELRTSEHYFFKLSDPRCEAFLRQYTSRDNGVLQNEAANKMQEWLGAPGENKLTDWDISRDA
PYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCARNGLDFNEYFKPDAKTELYHFIGKDILYFHALFWPAELAHAGFRTP
TKIFAHGFLTVDGAKMSKSRGTFITAESFLKTGLNPEWLRYYYAAKLSATMEDIDLSLDDFVARVNSDLVGKYVNIASRS
AGFITKRFNGQLAPATAELPAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHAA
CTNALNLFRLLTVLLKPILPIVAEKVEKFLNIAPLHWADTQSLLAGGHAINAYEHLMTRVDPKLIEKLVEANKESLAPAP
EAQSQQRHAEHQQNEVTAESPWEPFCNIDDFMKVDLRIVRIANAEHVEGADKLVRLTLDVGNNETRNVFAGIKAAYDPAQ
LIGRLTVMVANLAPRKMKFGLSEGMVLAASDGEGKTPGIFLLSPDSGAQPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=609, Percent_Identity=27.2577996715928, Blast_Score=229, Evalue=1e-59,
Organism=Homo sapiens, GI24308436, Length=533, Percent_Identity=23.6397748592871, Blast_Score=137, Evalue=4e-32,
Organism=Escherichia coli, GI1788432, Length=699, Percent_Identity=57.3676680972818, Blast_Score=809, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=589, Percent_Identity=28.1833616298812, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=25.2873563218391, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=417, Percent_Identity=25.4196642685851, Blast_Score=116, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=29.9465240641711, Blast_Score=257, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=22.1415607985481, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24655311, Length=571, Percent_Identity=28.7215411558669, Blast_Score=231, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358483, Length=531, Percent_Identity=24.4821092278719, Blast_Score=136, Evalue=6e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_DECAR (Q47IK0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283801.1
- ProteinModelPortal:   Q47IK0
- STRING:   Q47IK0
- GeneID:   3568800
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0574
- NMPDR:   fig|159087.4.peg.384
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   DARO159087:DARO_0574-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77848; Mature: 77717

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECWYVCADDTHGTPIMLRA
CCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCEEEEEE
EKEGITPEQLIARVHGEHSRDFAGFHVGFDNYYSTHSDETRDCANDIYLKLRAAGLIETR
CCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHEEEEECCCCHHHH
TIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCGAAYAPTDLKEPYSAISGAKP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHCCCCC
ELRTSEHYFFKLSDPRCEAFLRQYTSRDNGVLQNEAANKMQEWLGAPGENKLTDWDISRD
CCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCARNGLDFNEYFKPDAKTELYHFIGKD
CCCCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHH
ILYFHALFWPAELAHAGFRTPTKIFAHGFLTVDGAKMSKSRGTFITAESFLKTGLNPEWL
HHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCHHCCCCCCEEEHHHHHHCCCCHHHH
RYYYAAKLSATMEDIDLSLDDFVARVNSDLVGKYVNIASRSAGFITKRFNGQLAPATAEL
HHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHH
ACTNALNLFRLLTVLLKPILPIVAEKVEKFLNIAPLHWADTQSLLAGGHAINAYEHLMTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VDPKLIEKLVEANKESLAPAPEAQSQQRHAEHQQNEVTAESPWEPFCNIDDFMKVDLRIV
CCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCEEEE
RIANAEHVEGADKLVRLTLDVGNNETRNVFAGIKAAYDPAQLIGRLTVMVANLAPRKMKF
EECCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHC
GLSEGMVLAASDGEGKTPGIFLLSPDSGAQPGMRVK
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECWYVCADDTHGTPIMLRA
CCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCEEEEEE
EKEGITPEQLIARVHGEHSRDFAGFHVGFDNYYSTHSDETRDCANDIYLKLRAAGLIETR
CCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHEEEEECCCCHHHH
TIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCGAAYAPTDLKEPYSAISGAKP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHCCCCC
ELRTSEHYFFKLSDPRCEAFLRQYTSRDNGVLQNEAANKMQEWLGAPGENKLTDWDISRD
CCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCARNGLDFNEYFKPDAKTELYHFIGKD
CCCCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHH
ILYFHALFWPAELAHAGFRTPTKIFAHGFLTVDGAKMSKSRGTFITAESFLKTGLNPEWL
HHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCHHCCCCCCEEEHHHHHHCCCCHHHH
RYYYAAKLSATMEDIDLSLDDFVARVNSDLVGKYVNIASRSAGFITKRFNGQLAPATAEL
HHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHH
ACTNALNLFRLLTVLLKPILPIVAEKVEKFLNIAPLHWADTQSLLAGGHAINAYEHLMTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VDPKLIEKLVEANKESLAPAPEAQSQQRHAEHQQNEVTAESPWEPFCNIDDFMKVDLRIV
CCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCEEEE
RIANAEHVEGADKLVRLTLDVGNNETRNVFAGIKAAYDPAQLIGRLTVMVANLAPRKMKF
EECCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHC
GLSEGMVLAASDGEGKTPGIFLLSPDSGAQPGMRVK
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA