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The map label for this gene is proA

Identifier: 71906180

GI number: 71906180

Start: 598011

End: 599267

Strand: Reverse

Name: proA

Synonym: Daro_0540

Alternate gene names: 71906180

Gene position: 599267-598011 (Counterclockwise)

Preceding gene: 71906182

Following gene: 71906179

Centisome position: 13.31

GC content: 66.27

Gene sequence:

>1257_bases
ATGGACATCAAGCACTACATGCACACCGTCGGCCGCCAGGCCCGTGCTGCTTCACGCCGCCTGGCCGGCGCCACGACGGC
CGAAAAGAATGCTGCCTTGCTCGCCATCGCCGCCGCCATCCGCCGCGAAAGTGCCGCCCTGGTCGCCGCCAACCAGGAAG
ACCTGACCGCCGCCCGTGCTGCCGGCCTCGAAACTGCCATGCTCGACCGCCTGACGCTATCGGCCAAGGGCGTTGAAAGC
ATGGCCGAGGGCGTAGAGCAGGTGGCCACGCTGGCCGACCCGATCGGCGAGATGACCGACATCAAGTACCGCCCATCCGG
CATCCAGGTCGGCAAGATGCGCGTGCCGCTCGGCGTCATCGGCATCATTTACGAGGCTCGCCCGAACGTCACCGCCGATG
CCGCGGCACTGTGCCTGAAATCCGGCAACGCCGCCATCCTGCGCGGTGGCTCGGAAGCCATCCGCTCCAACCGCGCCATC
GCCGCACTGGTCCATGAAGGCCTGAAGGCGGCCGGCCTGCCGGCCGAATCGGTACAGGTCATCGACACCACCGACCGCGC
CGCCGTCGGCGAGCTGATCACCATGCGCGAATTCGTCGATGTCATCGTGCCGCGCGGCGGCAAGGGCCTGATCGCCCGCC
TGCTGGCCGAGTCGCGCGTGCCGATGATCCAGCACCTGGACGGCAACTGCCACGTCTATCTGGAAGCAGAAGCCGACCCG
GCCAAGGCGCTGAAGATCGTCGAGAATGCCAAGACCCAACGCTACGGCACCTGCAACACGGCCGAATCGCTGCTCGTCGA
CCGCTCGGTCGCCGCCATGCTGCTGCCGCCGATCGCCGCCATGCTGACCGCCAAGGGCGTCGAGATCCGCGGCTGCGCCG
AAACGCAAGCCATCGTGCCGAACGCCATCGCCGCCACGGAAGAGGACTACTACACCGAATACCTGGCGCCGATCATCTCG
GTCAAGGTCGTCTCCGGCATCGATGAAGCGATCGAGCACATCAACCAGTACTCGTCGCACCACAGCGAGGCCATCATTAC
CGACAACCATCCGAAGGCCATGCGTTTCCTGCGCGAAGTGGATTCGGCCTCGGTCATGATCAACGCCTCGACGCGCTTCG
CCGATGGCTTCGAGTACGGCCTGGGCGCCGAAATCGGCATCTCGACCGACAAGATCCACGCTCGCGGCCCGGTCGGTCTG
GAAGGGCTGACCAGCCAGAAGTGGGTGGTACTCGGCGACGGCCACGTCCGAGGCTAA

Upstream 100 bases:

>100_bases
GAAAGCCCTGTTGTCGTTGCGGATAAGAGTTTACCCTGTTCAGGAAGTTCCCCGTAATTTGTCTGGCTATAATGTTTCTT
TGCCCAGCACAAAGAACATC

Downstream 100 bases:

>100_bases
GCCGCTTCCGTCGACTCAACACAAGCATCCCCGCTCCGGCGGGGATGCGCTTTTCGGAGAAACCACATGGCCAAAGCCAG
TTTCAACTGGGAAGACCCGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MDIKHYMHTVGRQARAASRRLAGATTAEKNAALLAIAAAIRRESAALVAANQEDLTAARAAGLETAMLDRLTLSAKGVES
MAEGVEQVATLADPIGEMTDIKYRPSGIQVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRSNRAI
AALVHEGLKAAGLPAESVQVIDTTDRAAVGELITMREFVDVIVPRGGKGLIARLLAESRVPMIQHLDGNCHVYLEAEADP
AKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLPPIAAMLTAKGVEIRGCAETQAIVPNAIAATEEDYYTEYLAPIIS
VKVVSGIDEAIEHINQYSSHHSEAIITDNHPKAMRFLREVDSASVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGL
EGLTSQKWVVLGDGHVRG

Sequences:

>Translated_418_residues
MDIKHYMHTVGRQARAASRRLAGATTAEKNAALLAIAAAIRRESAALVAANQEDLTAARAAGLETAMLDRLTLSAKGVES
MAEGVEQVATLADPIGEMTDIKYRPSGIQVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRSNRAI
AALVHEGLKAAGLPAESVQVIDTTDRAAVGELITMREFVDVIVPRGGKGLIARLLAESRVPMIQHLDGNCHVYLEAEADP
AKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLPPIAAMLTAKGVEIRGCAETQAIVPNAIAATEEDYYTEYLAPIIS
VKVVSGIDEAIEHINQYSSHHSEAIITDNHPKAMRFLREVDSASVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGL
EGLTSQKWVVLGDGHVRG
>Mature_418_residues
MDIKHYMHTVGRQARAASRRLAGATTAEKNAALLAIAAAIRRESAALVAANQEDLTAARAAGLETAMLDRLTLSAKGVES
MAEGVEQVATLADPIGEMTDIKYRPSGIQVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRSNRAI
AALVHEGLKAAGLPAESVQVIDTTDRAAVGELITMREFVDVIVPRGGKGLIARLLAESRVPMIQHLDGNCHVYLEAEADP
AKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLPPIAAMLTAKGVEIRGCAETQAIVPNAIAATEEDYYTEYLAPIIS
VKVVSGIDEAIEHINQYSSHHSEAIITDNHPKAMRFLREVDSASVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGL
EGLTSQKWVVLGDGHVRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=412, Percent_Identity=35.1941747572816, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI21361368, Length=412, Percent_Identity=35.1941747572816, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=45.5421686746988, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17569967, Length=410, Percent_Identity=36.0975609756098, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17569969, Length=410, Percent_Identity=36.0975609756098, Blast_Score=275, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=36.9767441860465, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI21357643, Length=407, Percent_Identity=36.3636363636364, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_DECAR (Q47IN4)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283767.1
- ProteinModelPortal:   Q47IN4
- SMR:   Q47IN4
- STRING:   Q47IN4
- GeneID:   3570053
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0540
- NMPDR:   fig|159087.4.peg.350
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- BioCyc:   DARO159087:DARO_0540-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44269; Mature: 44269

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKHYMHTVGRQARAASRRLAGATTAEKNAALLAIAAAIRRESAALVAANQEDLTAARA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
AGLETAMLDRLTLSAKGVESMAEGVEQVATLADPIGEMTDIKYRPSGIQVGKMRVPLGVI
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCEEEE
GIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRSNRAIAALVHEGLKAAGLPAESVQV
EEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEE
IDTTDRAAVGELITMREFVDVIVPRGGKGLIARLLAESRVPMIQHLDGNCHVYLEAEADP
EECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHCCCCCEEEEEEECCCH
AKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLPPIAAMLTAKGVEIRGCAETQAIVP
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCC
NAIAATEEDYYTEYLAPIISVKVVSGIDEAIEHINQYSSHHSEAIITDNHPKAMRFLREV
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
DSASVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGLEGLTSQKWVVLGDGHVRG
CCCEEEEECCCCCCCCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MDIKHYMHTVGRQARAASRRLAGATTAEKNAALLAIAAAIRRESAALVAANQEDLTAARA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
AGLETAMLDRLTLSAKGVESMAEGVEQVATLADPIGEMTDIKYRPSGIQVGKMRVPLGVI
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCEEEE
GIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRSNRAIAALVHEGLKAAGLPAESVQV
EEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEE
IDTTDRAAVGELITMREFVDVIVPRGGKGLIARLLAESRVPMIQHLDGNCHVYLEAEADP
EECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHCCCCCEEEEEEECCCH
AKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLPPIAAMLTAKGVEIRGCAETQAIVP
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHCC
NAIAATEEDYYTEYLAPIISVKVVSGIDEAIEHINQYSSHHSEAIITDNHPKAMRFLREV
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHC
DSASVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGLEGLTSQKWVVLGDGHVRG
CCCEEEEECCCCCCCCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA