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The map label for this gene is dnaG [H]

Identifier: 71906167

GI number: 71906167

Start: 588141

End: 589877

Strand: Reverse

Name: dnaG [H]

Synonym: Daro_0527

Alternate gene names: 71906167

Gene position: 589877-588141 (Counterclockwise)

Preceding gene: 71906168

Following gene: 71906166

Centisome position: 13.11

GC content: 63.67

Gene sequence:

>1737_bases
GTGATTCCTGATTCGTTTATTCAGGACTTGCTGGCCCGGGTCGACATTGTCGACCTGGTAGATGGCTACGTACCGCTGAA
GAAAGCCGGCGCCAACTACGCTGCCTGCTGCCCGTTCCACAACGAGAAATCGCCGTCCTTCACGGTCAGCCCGACCAAGC
AGTTCTACCACTGCTTCGGCTGCGGCGCCCACGGTACGGCGATCAGTTTCGTCATGGAATACCAGGGCCTCGGCTTTGTC
GATGCCGTCAAGGATCTGGCCACCCGCGCCGGCATGCAGGTGCCGGAAAGCGAAGGGCGCAGCTTCAAGGACGAAAAGCC
GGGTCAGACGCGGACGCTGATCGAAATCATGGCCCGCGCCGCGGCCTACTACAAGGATCAGCTGAAGAAGTCGCCGCACG
CCATCGAGTACTGCAAGAAGCGCGGCCTGACCGGCGAAATCGCCGCCCGCTACGGCATCGGCTATGCCCCGGACGGCTGG
CAGAACCTGCAGGAAGTCTTCCCTGACTACAACGCCGACGAGCTGAAAATCGCCGGACTGGTCATCGAAAACGAAGCCGG
CCGCCGCTACGATCGCTTCCGTGACCGCCTGATGATCCCGATCATCAATCCGAAGGGCGAGATCATCGCCTTCGGCGGCC
GGATCATCGACCAGGGCGAGCCCAAATACCTGAATTCGCCGGAAACCCCGCTCTTCGAGAAGGGCCGCGAGCTGTTCGGC
CTCCCCCAAGCCCGCCAGGCCCTGCGCGAAACCGACACCGCCATCGTCACCGAAGGCTACATGGACGTCATTGCGCTGGC
CCAGAACGGCGTCGGCAATGCAGTCGCGACGCTCGGCACGGCGACCACGGCCACCCATGTCACCAAGCTGCTGCGCCAGG
TCGACCGCATCGTCTTCTGCTTTGACGGCGACAACGCCGGCCGCAAAGCGGCATGGCGGGCCCTGGAAAATTCGCTGGAA
GCCCTGGCCGACAACAAGCGCCTCGGCTTTATCTTCCTGCCCCAGGAGCATGACCCCGACAGCTACATCCGCGAATTCGG
CAAGGAACAGTTCGACCGGCAAGTCACTCAGGCCATGCCGCTCTCCGACTTCCTGCTGCGTGAACTGGCCCAGCGCTGCG
ACCTGACCAGCTCGGAAGGCAAGGCCCAGCTGATCTACGAGGCCAAGCCACTGCTGCTCAAGCTGCCGACCCCGCTGCTG
CGCCTGCAGCTGGTCAAGCGCCTGGCCGAAGCCAGCGGCTTTGCCCAGGCCGAAGTCGAGCGCCTGTGCGAACTGAAGTC
CTACACGCCGGCAGCGCCGCCCAAGGCCAAGCGCACCGCCCCGTCGCTGACCCGAAACCTGTTGCGCATCGTGCTGCACA
AACCGCAACTGGCCGCCCAGCTGCCGATAGCACTGCTGCCCGACACCCCGGAGCGTGCCGCCCTGACCCGCCTCCATCAG
CTGGTCACGGCCAATCGCGAGACCTCGAGCTACGCCGGTCTGCGCGAACAGCTGCGCGGACTGCCTGAAGAAAGCGTCAT
CGAAAATGCCGCCTCGGAACTTCTCGGCCTGCCGTTTGACGAAGAAGGGGCGGAGATGGAATTCCGCGACACGCTGGAAA
AACTTCAGGAGGGCGGCGAGAAGCGCGCCTTCGCCGAACTTCAGGTCAAGGCTCAGCAGTTCGGCGTGGCCGGACTGAGC
GCCGCCGAGAAACAGGCCTACATCCAGTTCCTCACCACCAAGGCAAATCGTAGCTAA

Upstream 100 bases:

>100_bases
GGGCAAGCTGATGGGCGTCCTGAAGCCCCGTCTGGCCGGCAAGGCCGACATGGCGGAAGTCTCGAAACTGGTCAAGGCGG
CGCTTGCCGGTTAAAAAGCG

Downstream 100 bases:

>100_bases
GTTGTGGTAAAATCTACGGTTTTCTCGAATTCTATGGATCGTGTTCATGGCTAAGGCAAAAGACACCGCTAAGGAAGCCC
CGAAGGCTCGCACCAGCAAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MIPDSFIQDLLARVDIVDLVDGYVPLKKAGANYAACCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAISFVMEYQGLGFV
DAVKDLATRAGMQVPESEGRSFKDEKPGQTRTLIEIMARAAAYYKDQLKKSPHAIEYCKKRGLTGEIAARYGIGYAPDGW
QNLQEVFPDYNADELKIAGLVIENEAGRRYDRFRDRLMIPIINPKGEIIAFGGRIIDQGEPKYLNSPETPLFEKGRELFG
LPQARQALRETDTAIVTEGYMDVIALAQNGVGNAVATLGTATTATHVTKLLRQVDRIVFCFDGDNAGRKAAWRALENSLE
ALADNKRLGFIFLPQEHDPDSYIREFGKEQFDRQVTQAMPLSDFLLRELAQRCDLTSSEGKAQLIYEAKPLLLKLPTPLL
RLQLVKRLAEASGFAQAEVERLCELKSYTPAAPPKAKRTAPSLTRNLLRIVLHKPQLAAQLPIALLPDTPERAALTRLHQ
LVTANRETSSYAGLREQLRGLPEESVIENAASELLGLPFDEEGAEMEFRDTLEKLQEGGEKRAFAELQVKAQQFGVAGLS
AAEKQAYIQFLTTKANRS

Sequences:

>Translated_578_residues
MIPDSFIQDLLARVDIVDLVDGYVPLKKAGANYAACCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAISFVMEYQGLGFV
DAVKDLATRAGMQVPESEGRSFKDEKPGQTRTLIEIMARAAAYYKDQLKKSPHAIEYCKKRGLTGEIAARYGIGYAPDGW
QNLQEVFPDYNADELKIAGLVIENEAGRRYDRFRDRLMIPIINPKGEIIAFGGRIIDQGEPKYLNSPETPLFEKGRELFG
LPQARQALRETDTAIVTEGYMDVIALAQNGVGNAVATLGTATTATHVTKLLRQVDRIVFCFDGDNAGRKAAWRALENSLE
ALADNKRLGFIFLPQEHDPDSYIREFGKEQFDRQVTQAMPLSDFLLRELAQRCDLTSSEGKAQLIYEAKPLLLKLPTPLL
RLQLVKRLAEASGFAQAEVERLCELKSYTPAAPPKAKRTAPSLTRNLLRIVLHKPQLAAQLPIALLPDTPERAALTRLHQ
LVTANRETSSYAGLREQLRGLPEESVIENAASELLGLPFDEEGAEMEFRDTLEKLQEGGEKRAFAELQVKAQQFGVAGLS
AAEKQAYIQFLTTKANRS
>Mature_578_residues
MIPDSFIQDLLARVDIVDLVDGYVPLKKAGANYAACCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAISFVMEYQGLGFV
DAVKDLATRAGMQVPESEGRSFKDEKPGQTRTLIEIMARAAAYYKDQLKKSPHAIEYCKKRGLTGEIAARYGIGYAPDGW
QNLQEVFPDYNADELKIAGLVIENEAGRRYDRFRDRLMIPIINPKGEIIAFGGRIIDQGEPKYLNSPETPLFEKGRELFG
LPQARQALRETDTAIVTEGYMDVIALAQNGVGNAVATLGTATTATHVTKLLRQVDRIVFCFDGDNAGRKAAWRALENSLE
ALADNKRLGFIFLPQEHDPDSYIREFGKEQFDRQVTQAMPLSDFLLRELAQRCDLTSSEGKAQLIYEAKPLLLKLPTPLL
RLQLVKRLAEASGFAQAEVERLCELKSYTPAAPPKAKRTAPSLTRNLLRIVLHKPQLAAQLPIALLPDTPERAALTRLHQ
LVTANRETSSYAGLREQLRGLPEESVIENAASELLGLPFDEEGAEMEFRDTLEKLQEGGEKRAFAELQVKAQQFGVAGLS
AAEKQAYIQFLTTKANRS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=546, Percent_Identity=42.6739926739927, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 64111; Mature: 64111

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDSFIQDLLARVDIVDLVDGYVPLKKAGANYAACCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAISFVMEYQGLGFVDAVKDLATRAGMQVPESEGRSFKDEKPGQTRTLIEIMARA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
AAYYKDQLKKSPHAIEYCKKRGLTGEIAARYGIGYAPDGWQNLQEVFPDYNADELKIAGL
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEE
VIENEAGRRYDRFRDRLMIPIINPKGEIIAFGGRIIDQGEPKYLNSPETPLFEKGRELFG
EEECCCCHHHHHHHHCEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHHHCC
LPQARQALRETDTAIVTEGYMDVIALAQNGVGNAVATLGTATTATHVTKLLRQVDRIVFC
CHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCEEEE
FDGDNAGRKAAWRALENSLEALADNKRLGFIFLPQEHDPDSYIREFGKEQFDRQVTQAMP
ECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
LSDFLLRELAQRCDLTSSEGKAQLIYEAKPLLLKLPTPLLRLQLVKRLAEASGFAQAEVE
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHH
RLCELKSYTPAAPPKAKRTAPSLTRNLLRIVLHKPQLAAQLPIALLPDTPERAALTRLHQ
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCEEECCCCCHHHHHHHHHH
LVTANRETSSYAGLREQLRGLPEESVIENAASELLGLPFDEEGAEMEFRDTLEKLQEGGE
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCH
KRAFAELQVKAQQFGVAGLSAAEKQAYIQFLTTKANRS
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPDSFIQDLLARVDIVDLVDGYVPLKKAGANYAACCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAISFVMEYQGLGFVDAVKDLATRAGMQVPESEGRSFKDEKPGQTRTLIEIMARA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
AAYYKDQLKKSPHAIEYCKKRGLTGEIAARYGIGYAPDGWQNLQEVFPDYNADELKIAGL
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEE
VIENEAGRRYDRFRDRLMIPIINPKGEIIAFGGRIIDQGEPKYLNSPETPLFEKGRELFG
EEECCCCHHHHHHHHCEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHHHCC
LPQARQALRETDTAIVTEGYMDVIALAQNGVGNAVATLGTATTATHVTKLLRQVDRIVFC
CHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCEEEE
FDGDNAGRKAAWRALENSLEALADNKRLGFIFLPQEHDPDSYIREFGKEQFDRQVTQAMP
ECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
LSDFLLRELAQRCDLTSSEGKAQLIYEAKPLLLKLPTPLLRLQLVKRLAEASGFAQAEVE
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHH
RLCELKSYTPAAPPKAKRTAPSLTRNLLRIVLHKPQLAAQLPIALLPDTPERAALTRLHQ
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCEEECCCCCHHHHHHHHHH
LVTANRETSSYAGLREQLRGLPEESVIENAASELLGLPFDEEGAEMEFRDTLEKLQEGGE
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCH
KRAFAELQVKAQQFGVAGLSAAEKQAYIQFLTTKANRS
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]