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The map label for this gene is rpoD [H]

Identifier: 71906166

GI number: 71906166

Start: 586121

End: 588094

Strand: Reverse

Name: rpoD [H]

Synonym: Daro_0526

Alternate gene names: 71906166

Gene position: 588094-586121 (Counterclockwise)

Preceding gene: 71906167

Following gene: 71906165

Centisome position: 13.07

GC content: 59.98

Gene sequence:

>1974_bases
ATGGCTAAGGCAAAAGACACCGCTAAGGAAGCCCCGAAGGCTCGCACCAGCAAGGCCAAGGATAAAGCCGCCGAAAAGGC
GCTGCTCCAGGGCCAGATGGCTGAGACGCCGGAACCGCTTGACGCCGAAGCGCGCAAAATGCGCCTCAAGGCCCTGATCA
AGCTGGGCAAGGAACGCGGCTACCTGACTTACGCCGAAATCAACGATCACCTGCCGGATGACGTCGTCGATGCCGAAAGT
ATCGAAGCGATCATCTCGACTTTCAGCGAAATGAGCATCCAGGTCTTCGACGAAGCCCCGGCTGCAGAAGACCTGCTGAT
GTCCGACACTGCTGCGGTTGCCGCCGACGACGAGGAAGTCGAAGCGCAGGCCGAGCAGGCCCTGTCCACTGTCGACTCCG
AATTCGGCCGCACCACCGACCCGGTCCGCATGTACATGCGTGAAATGGGCTCGGTCGAACTCCTCACCCGCGAAGGCGAA
ATCGAGATCGCCAAGCGAATCGAGGAAGGCCTGAAGCACATGATCCAGGCGATTGCCGCCTGCCCGACGACGATTTCCGA
CATTCTCGAATTGGCCGCCAAGGTCGAATCCGATGAAATGCGCATCGACGAACTGGTCGACGGCCTGATCGACCCGAACG
CTGTCGAAGAAGCCCCGGCGGCCGAAATGCCGGAAGCCGAAGAAGACGAAGAAGAAGACGGCGATGACGACGGCGAGGGT
GCTGGCGGCGGCGCCGCTGCGGCCTCCCTGCTGCAACTGAAGACCGATGCCTTGGAGCGCTTCAAGAACATCAAGGGCAT
CCACGCCAAGATGCAGAAGATGCTCCCGACCAAGGGTTCGCACGACAAGGTGTACATCAAGCTGCTGCAACAGGTTTCCG
ACGAACTGATGAACATCCGCTTCACCTCGCGTTCGATCGAGCGCCTGTGCGATAGCGTGCGCGGCATGGTCGAGCAGGTT
CGCGGTTGCGAGCGCAAGATCCAGCAAATCTGCGTGGACCGCGTCAAGATGCCGCGCCCGCACTTCATCCAGTCCTTCCC
GGGCAATGAAATCAACCTCGACTGGGCCGATGCCGAAGTCGCTGCCGCCCCCAAGACCTACGTTGCCATCCTGACCCGCA
ACGCGCCGGACATCAAGGAAGAGCAGAAGAAACTGCTCGCCCTGCAGGACCGTATCGGCATTCCGCTCAAGGACCTGAAA
GACATCAACAAGCAGATGTCCACCGGCGAAGCCAAGGCCCGCCGCGCCAAGCGCGAAATGACCGAAGCCAACCTGCGTCT
GGTCATCTCGATCGCCAAGAAATACACCAACCGCGGCCTGCAGTTCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGA
TGAAGGCGGTGGACAAGTTCGAATACCGCCGCGGCTACAAGTTCTCCACCTACGCCACATGGTGGATCCGCCAGGCCATC
ACCCGCTCCATCGCCGACCAGGCACGGACCATCCGTATCCCCGTGCACATGATCGAAACGATCAACAAGATGAACCGGAT
CAGCCGCCAGATCCTGCAGGAAACGGGTGCCGAACCCGATCCGGCGACGCTAGCCAAGAAGATGGACATGCCGGAAGACA
AGATTCGCAAGATCATGAAGATTTCCAAGGAGCCAATCTCCATGGAAACCCCGATCGGCGACGACGACGACTCGCACCTC
GGCGACTTCATCGAAGACTCAGGCACCCTGGCCCCGGCCGATGCCGCGATGTATTCCAGCCTGCGCGGCGTCACCAAGGA
AATCCTCGACACGCTGACGACGAGGGAAGCCAAGGTACTGCGCATGCGCTTCGGCATCGAAATGAACACCGATCACACGC
TGGAAGAAGTCGGCAAGCAATTCGACGTCACCCGCGAACGTATCCGTCAGATAGAAGCCAAAGCCCTGCGCAAGCTGCGT
CACCCGACCCGCTCCGACAAGCTGCGCAGTTTCGTCGACACGAACAACAGTTAA

Upstream 100 bases:

>100_bases
GCCGAGAAACAGGCCTACATCCAGTTCCTCACCACCAAGGCAAATCGTAGCTAAGTTGTGGTAAAATCTACGGTTTTCTC
GAATTCTATGGATCGTGTTC

Downstream 100 bases:

>100_bases
GTTTTCCGGGCCTGTAGCTCAGTTGGTTAGAGCAGAGGACTCATAATCCTTTGGTCCACGGTTCAAGTCCGTGCAGGCCC
ACCAGCATCATCAAGGACTT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MAKAKDTAKEAPKARTSKAKDKAAEKALLQGQMAETPEPLDAEARKMRLKALIKLGKERGYLTYAEINDHLPDDVVDAES
IEAIISTFSEMSIQVFDEAPAAEDLLMSDTAAVAADDEEVEAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGE
IEIAKRIEEGLKHMIQAIAACPTTISDILELAAKVESDEMRIDELVDGLIDPNAVEEAPAAEMPEAEEDEEEDGDDDGEG
AGGGAAAASLLQLKTDALERFKNIKGIHAKMQKMLPTKGSHDKVYIKLLQQVSDELMNIRFTSRSIERLCDSVRGMVEQV
RGCERKIQQICVDRVKMPRPHFIQSFPGNEINLDWADAEVAAAPKTYVAILTRNAPDIKEEQKKLLALQDRIGIPLKDLK
DINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
TRSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAKKMDMPEDKIRKIMKISKEPISMETPIGDDDDSHL
GDFIEDSGTLAPADAAMYSSLRGVTKEILDTLTTREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLR
HPTRSDKLRSFVDTNNS

Sequences:

>Translated_657_residues
MAKAKDTAKEAPKARTSKAKDKAAEKALLQGQMAETPEPLDAEARKMRLKALIKLGKERGYLTYAEINDHLPDDVVDAES
IEAIISTFSEMSIQVFDEAPAAEDLLMSDTAAVAADDEEVEAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGE
IEIAKRIEEGLKHMIQAIAACPTTISDILELAAKVESDEMRIDELVDGLIDPNAVEEAPAAEMPEAEEDEEEDGDDDGEG
AGGGAAAASLLQLKTDALERFKNIKGIHAKMQKMLPTKGSHDKVYIKLLQQVSDELMNIRFTSRSIERLCDSVRGMVEQV
RGCERKIQQICVDRVKMPRPHFIQSFPGNEINLDWADAEVAAAPKTYVAILTRNAPDIKEEQKKLLALQDRIGIPLKDLK
DINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
TRSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAKKMDMPEDKIRKIMKISKEPISMETPIGDDDDSHL
GDFIEDSGTLAPADAAMYSSLRGVTKEILDTLTTREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLR
HPTRSDKLRSFVDTNNS
>Mature_656_residues
AKAKDTAKEAPKARTSKAKDKAAEKALLQGQMAETPEPLDAEARKMRLKALIKLGKERGYLTYAEINDHLPDDVVDAESI
EAIISTFSEMSIQVFDEAPAAEDLLMSDTAAVAADDEEVEAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEI
EIAKRIEEGLKHMIQAIAACPTTISDILELAAKVESDEMRIDELVDGLIDPNAVEEAPAAEMPEAEEDEEEDGDDDGEGA
GGGAAAASLLQLKTDALERFKNIKGIHAKMQKMLPTKGSHDKVYIKLLQQVSDELMNIRFTSRSIERLCDSVRGMVEQVR
GCERKIQQICVDRVKMPRPHFIQSFPGNEINLDWADAEVAAAPKTYVAILTRNAPDIKEEQKKLLALQDRIGIPLKDLKD
INKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
RSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAKKMDMPEDKIRKIMKISKEPISMETPIGDDDDSHLG
DFIEDSGTLAPADAAMYSSLRGVTKEILDTLTTREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRH
PTRSDKLRSFVDTNNS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=611, Percent_Identity=57.2831423895254, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=40.343347639485, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=25.6198347107438, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 73634; Mature: 73503

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKDTAKEAPKARTSKAKDKAAEKALLQGQMAETPEPLDAEARKMRLKALIKLGKERG
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
YLTYAEINDHLPDDVVDAESIEAIISTFSEMSIQVFDEAPAAEDLLMSDTAAVAADDEEV
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHCCCHHHH
EAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEEGLKHMIQAIAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
CPTTISDILELAAKVESDEMRIDELVDGLIDPNAVEEAPAAEMPEAEEDEEEDGDDDGEG
CCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
AGGGAAAASLLQLKTDALERFKNIKGIHAKMQKMLPTKGSHDKVYIKLLQQVSDELMNIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FTSRSIERLCDSVRGMVEQVRGCERKIQQICVDRVKMPRPHFIQSFPGNEINLDWADAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHH
AAAPKTYVAILTRNAPDIKEEQKKLLALQDRIGIPLKDLKDINKQMSTGEAKARRAKREM
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
TEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TRSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAKKMDMPEDKIRKIMK
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHH
ISKEPISMETPIGDDDDSHLGDFIEDSGTLAPADAAMYSSLRGVTKEILDTLTTREAKVL
HCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSDKLRSFVDTNNS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKAKDTAKEAPKARTSKAKDKAAEKALLQGQMAETPEPLDAEARKMRLKALIKLGKERG
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
YLTYAEINDHLPDDVVDAESIEAIISTFSEMSIQVFDEAPAAEDLLMSDTAAVAADDEEV
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHCCCHHHH
EAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEEGLKHMIQAIAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
CPTTISDILELAAKVESDEMRIDELVDGLIDPNAVEEAPAAEMPEAEEDEEEDGDDDGEG
CCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
AGGGAAAASLLQLKTDALERFKNIKGIHAKMQKMLPTKGSHDKVYIKLLQQVSDELMNIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FTSRSIERLCDSVRGMVEQVRGCERKIQQICVDRVKMPRPHFIQSFPGNEINLDWADAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHH
AAAPKTYVAILTRNAPDIKEEQKKLLALQDRIGIPLKDLKDINKQMSTGEAKARRAKREM
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
TEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAI
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TRSIADQARTIRIPVHMIETINKMNRISRQILQETGAEPDPATLAKKMDMPEDKIRKIMK
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHH
ISKEPISMETPIGDDDDSHLGDFIEDSGTLAPADAAMYSSLRGVTKEILDTLTTREAKVL
HCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSDKLRSFVDTNNS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA