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The map label for this gene is leuA [H]

Identifier: 71906151

GI number: 71906151

Start: 571512

End: 573218

Strand: Reverse

Name: leuA [H]

Synonym: Daro_0511

Alternate gene names: 71906151

Gene position: 573218-571512 (Counterclockwise)

Preceding gene: 71906155

Following gene: 71906150

Centisome position: 12.74

GC content: 62.45

Gene sequence:

>1707_bases
ATGATTGCCAACCCAGCCACCAAATACACCGCTTTTCCGCCGGTCCAGCTTGCCGACCGCCAGTGGCCGAACCGCACGAT
CACCCAGCCGCCGATCTGGATGAGCACCGATCTTCGCGATGGCAACCAATCTCTTTTCGAACCAATGAATGCCGAAAAGA
AGATGCGCATGTTCAAGCTGCTCTGCGACATCGGCTTCAAGGAAATCGAAATCGCCTTCCCGTCCGCCTCGGAAACCGAA
TTCGGCTTCGTCCGCAATCTCATCGAAGGCGGCCACATTCCCGACGACGTCACCATCGAGGTCCTCACCCAGGCCCGCGA
ACACCTCATTCGCCGCACCTTCGAAGCACTCAAGGGCGCCAAGAAAGCCATCGTCCACGTCTATAACGCGACCAGCAAAA
CCTTCCGCGACAACGTCTTCGGCATGAGCAAGGCCGAGGTCGTGCAGATGGCCGTCGATGCCGTCAAGCTCATCCGTGCG
CAGGCGGAAGCCATGCCGGACACCGAAATCATCCTCGAATACAGCCCGGAACTCTTTACCGCCACCGAGCTCGATTTCGC
CAAGGAAGTCTGCGATGCGGTCACCACCGCCTGGGGTGCGACGCCGCAGCGCAAGGTCATCCTCAACCTTCCGACCACGG
TCGAGATCGCTACGCCCAACATCTACGCCGACCAGATCGAGTGGATGCACCGCAACCTCGCCCGGCGCGACAGCGTGATC
ATCAGTCTCCACCCGCACAACGACCGCGGCACCGCCGTCGCCGCCGCCGAACTCGGCCTGATGGCCGGCGCCGATCGCGT
CGAGGGCTGCCTCTTCGGCAATGGTGAGCGTACCGGTAACGTCGACCTCGTCACCGTCGCCCTCAACATGTACACGCAAG
GCGTCCATCCCGGCCTTGATTTCTCGGACATCAATGCCGTCGCCCGTGCTTTCGAACACTGTAACCAGCTCCCGATCCAC
CCACGCCACCCATACGTCGGGGATCTCGTTTTCACGGCCTTCTCCGGCTCCCACCAGGACGCCATCAAGAAGGGGTTTTC
CGCTCAAGAGGCGGCAGAGCAATGGTCAGTGCCCTACCTCCCGATTGACCCGGCCGACGTTGGCCGCAGCTACGACTCGG
TCATCCGCGTCAACAGCCAGTCGGGCAAGGGGGGCATCGCCTATTTGATGGAAACCGAACACGGTGTGGTCATGCCGCGC
CGCTTGCAGGTCGAGTTCTCCGGCGTCGTCCAGCAACACACCGACGCCCATGGCGGCGAAGTGAGCGCTGACGGCATCTG
GGCCATGTTCGCCCGGACCTACCTCGACGCCACCACCCCGATCCGCTACCGCGAACACCACCTCTTCGAACAAGGGCGCA
ATCAGGGCATCCGCTTGAACATCGACATCGACGGCACGCCACACATCCTGACCGGCGAAGGCAACGGCCCGATCAACGCC
GCAGTCCACGCCCTGCAGAGCGCCGGCATCAACATTCAGGTGCGCAGCTACGAGGAACGTTCGATGGTACCGATGGGTGA
AGATGGCAACGCCCGGGCCTGCGCCTTCATGGAAATCGCCGGCAAGGGTGGAGACAAGAGCGGCGGCGAATGCTACGGCG
TCGGCGTCGACAGCAATATCGTCACCGCGTCGATCAAGGCCTTGCTCAGTGGTGTCAATCGCCTCGGCGCCGCCCGGACG
AGCGGCGACCAATCGCAAGCCGCCTGA

Upstream 100 bases:

>100_bases
ATCTGCCAATTTAATGCAAATTCATAGCCAATTTCGCAAGCACCTGCCGCTCGCTTTAGCCTAATATTTCGCCATCGCAA
AACGTTTTCCGGAGCACATC

Downstream 100 bases:

>100_bases
GGCACCACGCAATCCCTTGCCAAAACATCGGCAAGGGATTGAATTTTCTAATACTTTCCTTGCTTACCGGCATTTCGGCG
CGTAGAATTCTTCCAGCCTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MIANPATKYTAFPPVQLADRQWPNRTITQPPIWMSTDLRDGNQSLFEPMNAEKKMRMFKLLCDIGFKEIEIAFPSASETE
FGFVRNLIEGGHIPDDVTIEVLTQAREHLIRRTFEALKGAKKAIVHVYNATSKTFRDNVFGMSKAEVVQMAVDAVKLIRA
QAEAMPDTEIILEYSPELFTATELDFAKEVCDAVTTAWGATPQRKVILNLPTTVEIATPNIYADQIEWMHRNLARRDSVI
ISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVHPGLDFSDINAVARAFEHCNQLPIH
PRHPYVGDLVFTAFSGSHQDAIKKGFSAQEAAEQWSVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAYLMETEHGVVMPR
RLQVEFSGVVQQHTDAHGGEVSADGIWAMFARTYLDATTPIRYREHHLFEQGRNQGIRLNIDIDGTPHILTGEGNGPINA
AVHALQSAGINIQVRSYEERSMVPMGEDGNARACAFMEIAGKGGDKSGGECYGVGVDSNIVTASIKALLSGVNRLGAART
SGDQSQAA

Sequences:

>Translated_568_residues
MIANPATKYTAFPPVQLADRQWPNRTITQPPIWMSTDLRDGNQSLFEPMNAEKKMRMFKLLCDIGFKEIEIAFPSASETE
FGFVRNLIEGGHIPDDVTIEVLTQAREHLIRRTFEALKGAKKAIVHVYNATSKTFRDNVFGMSKAEVVQMAVDAVKLIRA
QAEAMPDTEIILEYSPELFTATELDFAKEVCDAVTTAWGATPQRKVILNLPTTVEIATPNIYADQIEWMHRNLARRDSVI
ISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVHPGLDFSDINAVARAFEHCNQLPIH
PRHPYVGDLVFTAFSGSHQDAIKKGFSAQEAAEQWSVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAYLMETEHGVVMPR
RLQVEFSGVVQQHTDAHGGEVSADGIWAMFARTYLDATTPIRYREHHLFEQGRNQGIRLNIDIDGTPHILTGEGNGPINA
AVHALQSAGINIQVRSYEERSMVPMGEDGNARACAFMEIAGKGGDKSGGECYGVGVDSNIVTASIKALLSGVNRLGAART
SGDQSQAA
>Mature_568_residues
MIANPATKYTAFPPVQLADRQWPNRTITQPPIWMSTDLRDGNQSLFEPMNAEKKMRMFKLLCDIGFKEIEIAFPSASETE
FGFVRNLIEGGHIPDDVTIEVLTQAREHLIRRTFEALKGAKKAIVHVYNATSKTFRDNVFGMSKAEVVQMAVDAVKLIRA
QAEAMPDTEIILEYSPELFTATELDFAKEVCDAVTTAWGATPQRKVILNLPTTVEIATPNIYADQIEWMHRNLARRDSVI
ISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVHPGLDFSDINAVARAFEHCNQLPIH
PRHPYVGDLVFTAFSGSHQDAIKKGFSAQEAAEQWSVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAYLMETEHGVVMPR
RLQVEFSGVVQQHTDAHGGEVSADGIWAMFARTYLDATTPIRYREHHLFEQGRNQGIRLNIDIDGTPHILTGEGNGPINA
AVHALQSAGINIQVRSYEERSMVPMGEDGNARACAFMEIAGKGGDKSGGECYGVGVDSNIVTASIKALLSGVNRLGAART
SGDQSQAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=369, Percent_Identity=26.2872628726287, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=577, Percent_Identity=47.6603119584055, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6324682, Length=572, Percent_Identity=47.5524475524476, Blast_Score=534, Evalue=1e-152,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62058; Mature: 62058

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANPATKYTAFPPVQLADRQWPNRTITQPPIWMSTDLRDGNQSLFEPMNAEKKMRMFKL
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHH
LCDIGFKEIEIAFPSASETEFGFVRNLIEGGHIPDDVTIEVLTQAREHLIRRTFEALKGA
HHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
KKAIVHVYNATSKTFRDNVFGMSKAEVVQMAVDAVKLIRAQAEAMPDTEIILEYSPELFT
HHHEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
ATELDFAKEVCDAVTTAWGATPQRKVILNLPTTVEIATPNIYADQIEWMHRNLARRDSVI
EHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEE
ISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVHPGLD
EEEECCCCCCCEEHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDINAVARAFEHCNQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFSAQEAAEQWSVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHCCCCCC
PIDPADVGRSYDSVIRVNSQSGKGGIAYLMETEHGVVMPRRLQVEFSGVVQQHTDAHGGE
CCCHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEECCEEEEEEHHHHHHHHCCCCCCE
VSADGIWAMFARTYLDATTPIRYREHHLFEQGRNQGIRLNIDIDGTPHILTGEGNGPINA
ECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCHHH
AVHALQSAGINIQVRSYEERSMVPMGEDGNARACAFMEIAGKGGDKSGGECYGVGVDSNI
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCH
VTASIKALLSGVNRLGAARTSGDQSQAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIANPATKYTAFPPVQLADRQWPNRTITQPPIWMSTDLRDGNQSLFEPMNAEKKMRMFKL
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHH
LCDIGFKEIEIAFPSASETEFGFVRNLIEGGHIPDDVTIEVLTQAREHLIRRTFEALKGA
HHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
KKAIVHVYNATSKTFRDNVFGMSKAEVVQMAVDAVKLIRAQAEAMPDTEIILEYSPELFT
HHHEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
ATELDFAKEVCDAVTTAWGATPQRKVILNLPTTVEIATPNIYADQIEWMHRNLARRDSVI
EHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEE
ISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVHPGLD
EEEECCCCCCCEEHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDINAVARAFEHCNQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFSAQEAAEQWSVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHHHCCCCCC
PIDPADVGRSYDSVIRVNSQSGKGGIAYLMETEHGVVMPRRLQVEFSGVVQQHTDAHGGE
CCCHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEECCEEEEEEHHHHHHHHCCCCCCE
VSADGIWAMFARTYLDATTPIRYREHHLFEQGRNQGIRLNIDIDGTPHILTGEGNGPINA
ECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCHHH
AVHALQSAGINIQVRSYEERSMVPMGEDGNARACAFMEIAGKGGDKSGGECYGVGVDSNI
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCH
VTASIKALLSGVNRLGAARTSGDQSQAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA