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The map label for this gene is lpdA [H]

Identifier: 71906080

GI number: 71906080

Start: 482327

End: 484102

Strand: Reverse

Name: lpdA [H]

Synonym: Daro_0440

Alternate gene names: 71906080

Gene position: 484102-482327 (Counterclockwise)

Preceding gene: 71906081

Following gene: 71906077

Centisome position: 10.76

GC content: 63.96

Gene sequence:

>1776_bases
ATGAGCAATCTGGTAGAAGTCAAAGTCCCCGATATCGGCGATTTCGCCGAAGTGCCGATCATCGACCTGTTCGTCAAGGT
CGGCGACAGCATCAAGGTGGATGACGCGATCTGCACGCTGGAATCCGACAAGGCGACGATGGATGTACCGTCGCCGGTCG
CCGGTGTGGTCAAGGAAGTGCTTGTTCAGCTCGGCGCCAAGGTGGCCGAAGGCTCCTTGCTGATCAAGGTCGAATCCGGT
GCTGCGGCGGCTGCCCCGGCCCCGCAAGCCGCCGCCCCCGCTGCCGCTCCTGCAGCGGTGCCGGTTGCTGCCCCGGTCGC
CGGCAGCCATGCCGGCGGTGCCGACATCGAGTGCGAAATGCTCGTCCTCGGCGCCGGCCCCGGCGGTTATTCCGCCGCCT
TCCGCTCGGCCGACCTCGGCATGAAGACGGTCATCGTCGAGCGCTACGCCACCCTCGGCGGCGTCTGCCTCAACGTCGGC
TGCATCCCGTCCAAGGCCCTGCTGCACGTCGCAGCCGTCATGGAAGAAGCCGAGCACGCCAATGACCTCGGCGTCACCTT
TGCCGCGCCGACTGTCGATATCGACAAGCTGCGCGCCCACAAGGACAAGGTCGTCGGCAAGCTGACCGGTGGTCTGGCCG
GCATGGCCAAGGGCCGCAAGGTGGAGATCGTGCGCGGCTACGGTACGTTCATCGACCCGCACCACATCGAGGTCGAAGTG
ACCGATGGCACGGGCCAGGACAAGACCGGCGCCAAGAAGATCGTCAAATTCCAGAAATGCATCATCGCCGCCGGTTCGGC
CGCCATGCATCTGCCCTTCATTCCGAAGGACCCGCGTATTGTCGATTCGACCGGGGCGCTCGAACTACGCTTCGTGCCGG
AGAAGATGCTGGTCATCGGCGGCGGCATCATCGGCCTCGAAATGGCCACGGTCTATTCGACGCTGGGCGCCAAGGTCGAT
GTCGTCGAAATGATGGATGGCCTGATGCAAGGCCCGGATCGCGACGCCGTCAAGGTCTGGGAGAAGCAGAACGCCAAACG
CTTCGACAAGATCATGCTGAAGACCAAGACGGTGGCTGTCGATGCCAAGGATGACGGCCTCTACGTCAAGTTTGAGGGCG
AAGGCGTCTCGCCGGAACCGGTCAAGTACGACATGATCCTGCAAGCCGCCGGCCGCACGCCGAACGGCAACAAGATCGGT
GCCGACAAGGCTGGCGTGGCCGTGACCGAGCGTGGCTTCATCAATGTTGATGCCCAGATGCGCACCAACGTGCCGCACAT
CTTCGCCATCGGCGACCTCGTCGGCCAGCCCATGCTGGCCCACAAGGCGGTGCATGAGTCGCACGTCGCGGCGGAAGCGG
CGGCGGGCCAGAAATCGGCCTTCGACGCCTCGGTGATCCCGAGTGTCGCCTACACCCACCCGGAAGTGGCATGGGTTGGT
GTCACCGAAGATCAGGCCAAGAAGGATGGTCGCAAGATCGAAGCCGCCAAGTTCCCGTGGGCAGCCTCCGGTCGTGCCAT
CGCCAACGGTGCCGATTACGGCTTCACCAAGCTGATCTTCGACGCCGAAACGCATCGCGTCATCGGCGGGGCGATTGTCG
GCCCGAACGCTGGCGACATGATCGGCGAAGTCTGTCTGGCCATCGAAATGGGCTGCGACTCGGTCGATATCGGCAAGACC
ATCCACCCGCACCCGACGCTGGGTGAAACGGTGGGCATGGCCGCCGAAGTGGCGCACGGCACCTGTACGGATGTTCCGGC
ACCGCGCAAGAAGTAA

Upstream 100 bases:

>100_bases
CCACCGCGTCATCGACGGTGCGCTGGCGACCCGCTTCAATGTCTATATCGCCCAGCTGCTGGCCGACTTCCGTCGCGTCG
CGCTGTAAAGGGGAATGACC

Downstream 100 bases:

>100_bases
CGACGCTAATCCATTCTTGGCGTTCGGTAAGGGCGATGGTTTCCACCGTCGCCCTTTCTTCATCTAACGGAAGTCTAGGG
CGCCTTACGCGCAGCCTTCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSNLVEVKVPDIGDFAEVPIIDLFVKVGDSIKVDDAICTLESDKATMDVPSPVAGVVKEVLVQLGAKVAEGSLLIKVESG
AAAAAPAPQAAAPAAAPAAVPVAAPVAGSHAGGADIECEMLVLGAGPGGYSAAFRSADLGMKTVIVERYATLGGVCLNVG
CIPSKALLHVAAVMEEAEHANDLGVTFAAPTVDIDKLRAHKDKVVGKLTGGLAGMAKGRKVEIVRGYGTFIDPHHIEVEV
TDGTGQDKTGAKKIVKFQKCIIAAGSAAMHLPFIPKDPRIVDSTGALELRFVPEKMLVIGGGIIGLEMATVYSTLGAKVD
VVEMMDGLMQGPDRDAVKVWEKQNAKRFDKIMLKTKTVAVDAKDDGLYVKFEGEGVSPEPVKYDMILQAAGRTPNGNKIG
ADKAGVAVTERGFINVDAQMRTNVPHIFAIGDLVGQPMLAHKAVHESHVAAEAAAGQKSAFDASVIPSVAYTHPEVAWVG
VTEDQAKKDGRKIEAAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGAIVGPNAGDMIGEVCLAIEMGCDSVDIGKT
IHPHPTLGETVGMAAEVAHGTCTDVPAPRKK

Sequences:

>Translated_591_residues
MSNLVEVKVPDIGDFAEVPIIDLFVKVGDSIKVDDAICTLESDKATMDVPSPVAGVVKEVLVQLGAKVAEGSLLIKVESG
AAAAAPAPQAAAPAAAPAAVPVAAPVAGSHAGGADIECEMLVLGAGPGGYSAAFRSADLGMKTVIVERYATLGGVCLNVG
CIPSKALLHVAAVMEEAEHANDLGVTFAAPTVDIDKLRAHKDKVVGKLTGGLAGMAKGRKVEIVRGYGTFIDPHHIEVEV
TDGTGQDKTGAKKIVKFQKCIIAAGSAAMHLPFIPKDPRIVDSTGALELRFVPEKMLVIGGGIIGLEMATVYSTLGAKVD
VVEMMDGLMQGPDRDAVKVWEKQNAKRFDKIMLKTKTVAVDAKDDGLYVKFEGEGVSPEPVKYDMILQAAGRTPNGNKIG
ADKAGVAVTERGFINVDAQMRTNVPHIFAIGDLVGQPMLAHKAVHESHVAAEAAAGQKSAFDASVIPSVAYTHPEVAWVG
VTEDQAKKDGRKIEAAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGAIVGPNAGDMIGEVCLAIEMGCDSVDIGKT
IHPHPTLGETVGMAAEVAHGTCTDVPAPRKK
>Mature_590_residues
SNLVEVKVPDIGDFAEVPIIDLFVKVGDSIKVDDAICTLESDKATMDVPSPVAGVVKEVLVQLGAKVAEGSLLIKVESGA
AAAAPAPQAAAPAAAPAAVPVAAPVAGSHAGGADIECEMLVLGAGPGGYSAAFRSADLGMKTVIVERYATLGGVCLNVGC
IPSKALLHVAAVMEEAEHANDLGVTFAAPTVDIDKLRAHKDKVVGKLTGGLAGMAKGRKVEIVRGYGTFIDPHHIEVEVT
DGTGQDKTGAKKIVKFQKCIIAAGSAAMHLPFIPKDPRIVDSTGALELRFVPEKMLVIGGGIIGLEMATVYSTLGAKVDV
VEMMDGLMQGPDRDAVKVWEKQNAKRFDKIMLKTKTVAVDAKDDGLYVKFEGEGVSPEPVKYDMILQAAGRTPNGNKIGA
DKAGVAVTERGFINVDAQMRTNVPHIFAIGDLVGQPMLAHKAVHESHVAAEAAAGQKSAFDASVIPSVAYTHPEVAWVGV
TEDQAKKDGRKIEAAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGAIVGPNAGDMIGEVCLAIEMGCDSVDIGKTI
HPHPTLGETVGMAAEVAHGTCTDVPAPRKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=40.5639913232104, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=26.6949152542373, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=27.8801843317972, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=28.2758620689655, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=28.2758620689655, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=28.2758620689655, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=27.8801843317972, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI291045266, Length=442, Percent_Identity=27.6018099547511, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=27.7542372881356, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=434, Percent_Identity=26.036866359447, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=61.9246861924686, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=28.9915966386555, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=26.1904761904762, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=27.027027027027, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786305, Length=71, Percent_Identity=49.2957746478873, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=40.04329004329, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=27.8825995807128, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=446, Percent_Identity=25.7847533632287, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=446, Percent_Identity=25.7847533632287, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=463, Percent_Identity=26.133909287257, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=467, Percent_Identity=38.9721627408994, Blast_Score=289, Evalue=9e-79,
Organism=Saccharomyces cerevisiae, GI6325240, Length=485, Percent_Identity=26.3917525773196, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.2151898734177, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI21358499, Length=461, Percent_Identity=41.2147505422993, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24640551, Length=539, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=28.8888888888889, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640553, Length=501, Percent_Identity=28.3433133732535, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.4462809917355, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61382; Mature: 61251

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLVEVKVPDIGDFAEVPIIDLFVKVGDSIKVDDAICTLESDKATMDVPSPVAGVVKEV
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHH
LVQLGAKVAEGSLLIKVESGAAAAAPAPQAAAPAAAPAAVPVAAPVAGSHAGGADIECEM
HHHHCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEE
LVLGAGPGGYSAAFRSADLGMKTVIVERYATLGGVCLNVGCIPSKALLHVAAVMEEAEHA
EEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCC
NDLGVTFAAPTVDIDKLRAHKDKVVGKLTGGLAGMAKGRKVEIVRGYGTFIDPHHIEVEV
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCEEEEEE
TDGTGQDKTGAKKIVKFQKCIIAAGSAAMHLPFIPKDPRIVDSTGALELRFVPEKMLVIG
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCEEEEECCCEEEEEE
GGIIGLEMATVYSTLGAKVDVVEMMDGLMQGPDRDAVKVWEKQNAKRFDKIMLKTKTVAV
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHEEEEEEEE
DAKDDGLYVKFEGEGVSPEPVKYDMILQAAGRTPNGNKIGADKAGVAVTERGFINVDAQM
ECCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECCHH
RTNVPHIFAIGDLVGQPMLAHKAVHESHVAAEAAAGQKSAFDASVIPSVAYTHPEVAWVG
HCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHCCCCCEEEEE
VTEDQAKKDGRKIEAAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGAIVGPNAGDM
CCHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCEEEEEEEECCCCHHH
IGEVCLAIEMGCDSVDIGKTIHPHPTLGETVGMAAEVAHGTCTDVPAPRKK
HHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNLVEVKVPDIGDFAEVPIIDLFVKVGDSIKVDDAICTLESDKATMDVPSPVAGVVKEV
CCEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHH
LVQLGAKVAEGSLLIKVESGAAAAAPAPQAAAPAAAPAAVPVAAPVAGSHAGGADIECEM
HHHHCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEE
LVLGAGPGGYSAAFRSADLGMKTVIVERYATLGGVCLNVGCIPSKALLHVAAVMEEAEHA
EEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCC
NDLGVTFAAPTVDIDKLRAHKDKVVGKLTGGLAGMAKGRKVEIVRGYGTFIDPHHIEVEV
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCEEEEEE
TDGTGQDKTGAKKIVKFQKCIIAAGSAAMHLPFIPKDPRIVDSTGALELRFVPEKMLVIG
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCEEEEECCCEEEEEE
GGIIGLEMATVYSTLGAKVDVVEMMDGLMQGPDRDAVKVWEKQNAKRFDKIMLKTKTVAV
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHEEEEEEEE
DAKDDGLYVKFEGEGVSPEPVKYDMILQAAGRTPNGNKIGADKAGVAVTERGFINVDAQM
ECCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECCHH
RTNVPHIFAIGDLVGQPMLAHKAVHESHVAAEAAAGQKSAFDASVIPSVAYTHPEVAWVG
HCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHCCCCCEEEEE
VTEDQAKKDGRKIEAAKFPWAASGRAIANGADYGFTKLIFDAETHRVIGGAIVGPNAGDM
CCHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCEEEEEEEECCCCHHH
IGEVCLAIEMGCDSVDIGKTIHPHPTLGETVGMAAEVAHGTCTDVPAPRKK
HHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]