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The map label for this gene is metK

Identifier: 71905830

GI number: 71905830

Start: 206325

End: 207488

Strand: Reverse

Name: metK

Synonym: Daro_0188

Alternate gene names: 71905830

Gene position: 207488-206325 (Counterclockwise)

Preceding gene: 71905848

Following gene: 71905829

Centisome position: 4.61

GC content: 63.23

Gene sequence:

>1164_bases
ATGAGCGATTACCTCTTCACTTCGGAATCTGTCGGCGAAGGCCATCCCGACAAGGTTGCCGACCAGATTTCCGATGCTAT
CCTCGACGCCATCCTGGCGCAGGACAAGCATTCCCGCGTTGCCGCCGAAACCCTGTGCAATACCGGTCTCGTTGTGCTGG
CCGGTGAAATCACCACCAACGCCAATGTCGACTACATCCAAGTCGCCCGCGACACCATCAAGCGCATCGGCTACGACAAC
ACCGAATACGGCATCGACTACAAAGGCTGTGCCGTGCTCGTCGCCTACGACAAGCAGAGCCCGGATATCGCCCAGGGCGT
CAATGCCGCCTATGACGACAACCTCGGACAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCTGCGATGAAACCC
CGTCGCTGATGCCGCTGCCAATCTACCTGTCGCACCGTATGGTCGAGCGCCAGGCCATGCTGCGCAAGGACGGCCGTCTG
CCGTGGGCTCGCCCGGACGCCAAGTCGCAGGTCACCATCCGCTACGTCGATGGCAAGCCGCACTCGATCGACACCGTCGT
GCTGTCCACCCAGCACTCGCCGGACATCAGCCTGGAAGACCTGCGCGAAGCCACCATCGAGCAGATCATCAAACCGGTGC
TGCCGAAGGAACTGATCAAGGGCGACATCAAGTACCTGGTCAACCCGACCGGCCGCTTCGTCGTCGGCGGCCCGCAGGGC
GACTGTGGCCTGACCGGCCGCAAGATCATCGTCGACACCTACGGTGGTGCCGCGCCGCACGGCGGTGGCGCCTTCTCCGG
CAAGGATCCCTCCAAGGTCGACCGTTCGGCTGCCTACGCCACCCGTTACGTCGCCAAGAACATTGTCGCCGCCGGTCTGG
CCTCACGCTGCCTGGTCCAGGTTTCCTACGCCATCGGCGTCGCTGAGCCAACCTCGATCATGGTCGAGACCTACGGCACC
GGCAAGGTCAGCAACGAAACCCTGACGGCGCTCGTTCGCAAGCACTTCGACCTGCGTCCGAAGGGCATCGTCAACATGCT
CGACCTGCTCCGTCCGATCTACCAGAAGACCGCCGCCTACGGCCATTTCGGTCGCGACGAGCCGGAATTCACCTGGGAAG
CGACCGACCGTGCCAACCTGCTGAAGAGTGACGCCGGCCTGTAA

Upstream 100 bases:

>100_bases
AACAGACAACTTGCGGGGCGACTAATAAATTCTGCTAAAGCGTCACCTGCTCGTGGTCCAAAGCTGGTAGCGCCGGATCA
AAGGACCACTGGAGCACTCC

Downstream 100 bases:

>100_bases
ATCCTACATTTCTTCGCATTAAACAAGGGGGCGCGCAGCCCCCTTTTTGTTAGAATTGCGAAATATGTTGAAGAAAATAC
CAGTCGAAAAACTGACGCTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSDYLFTSESVGEGHPDKVADQISDAILDAILAQDKHSRVAAETLCNTGLVVLAGEITTNANVDYIQVARDTIKRIGYDN
TEYGIDYKGCAVLVAYDKQSPDIAQGVNAAYDDNLGQGAGDQGLMFGYACDETPSLMPLPIYLSHRMVERQAMLRKDGRL
PWARPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDISLEDLREATIEQIIKPVLPKELIKGDIKYLVNPTGRFVVGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYVAKNIVAAGLASRCLVQVSYAIGVAEPTSIMVETYGT
GKVSNETLTALVRKHFDLRPKGIVNMLDLLRPIYQKTAAYGHFGRDEPEFTWEATDRANLLKSDAGL

Sequences:

>Translated_387_residues
MSDYLFTSESVGEGHPDKVADQISDAILDAILAQDKHSRVAAETLCNTGLVVLAGEITTNANVDYIQVARDTIKRIGYDN
TEYGIDYKGCAVLVAYDKQSPDIAQGVNAAYDDNLGQGAGDQGLMFGYACDETPSLMPLPIYLSHRMVERQAMLRKDGRL
PWARPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDISLEDLREATIEQIIKPVLPKELIKGDIKYLVNPTGRFVVGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYVAKNIVAAGLASRCLVQVSYAIGVAEPTSIMVETYGT
GKVSNETLTALVRKHFDLRPKGIVNMLDLLRPIYQKTAAYGHFGRDEPEFTWEATDRANLLKSDAGL
>Mature_386_residues
SDYLFTSESVGEGHPDKVADQISDAILDAILAQDKHSRVAAETLCNTGLVVLAGEITTNANVDYIQVARDTIKRIGYDNT
EYGIDYKGCAVLVAYDKQSPDIAQGVNAAYDDNLGQGAGDQGLMFGYACDETPSLMPLPIYLSHRMVERQAMLRKDGRLP
WARPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDISLEDLREATIEQIIKPVLPKELIKGDIKYLVNPTGRFVVGGPQGD
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYVAKNIVAAGLASRCLVQVSYAIGVAEPTSIMVETYGTG
KVSNETLTALVRKHFDLRPKGIVNMLDLLRPIYQKTAAYGHFGRDEPEFTWEATDRANLLKSDAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=60.427807486631, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=58.2887700534759, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=67.7002583979328, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=59.3582887700535, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=389, Percent_Identity=58.6118251928021, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=58.5561497326203, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=376, Percent_Identity=56.1170212765958, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.6219512195122, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=52.9411764705882, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=56.8513119533528, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=56.8106312292359, Blast_Score=320, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=60.9756097560976, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320710, Length=375, Percent_Identity=58.6666666666667, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=58.6666666666667, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=56.9148936170213, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=56.1497326203209, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=56.1497326203209, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=54.8476454293629, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=56.3025210084034, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_DECAR (Q47JN4)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283417.1
- ProteinModelPortal:   Q47JN4
- SMR:   Q47JN4
- STRING:   Q47JN4
- GeneID:   3569484
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0188
- NMPDR:   fig|159087.4.peg.301
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   DARO159087:DARO_0188-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41911; Mature: 41780

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLFTSESVGEGHPDKVADQISDAILDAILAQDKHSRVAAETLCNTGLVVLAGEITTN
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEEECC
ANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVNAAYDDNLGQGAG
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHCCCCCCCCC
DQGLMFGYACDETPSLMPLPIYLSHRMVERQAMLRKDGRLPWARPDAKSQVTIRYVDGKP
CCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCC
HSIDTVVLSTQHSPDISLEDLREATIEQIIKPVLPKELIKGDIKYLVNPTGRFVVGGPQG
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCHHEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYVAKNIVAAGLASRCLVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYAIGVAEPTSIMVETYGTGKVSNETLTALVRKHFDLRPKGIVNMLDLLRPIYQKTAAY
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GHFGRDEPEFTWEATDRANLLKSDAGL
CCCCCCCCCEEEECCCHHHHHHHCCCC
>Mature Secondary Structure 
SDYLFTSESVGEGHPDKVADQISDAILDAILAQDKHSRVAAETLCNTGLVVLAGEITTN
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEEECC
ANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVNAAYDDNLGQGAG
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHCCCCCCCCC
DQGLMFGYACDETPSLMPLPIYLSHRMVERQAMLRKDGRLPWARPDAKSQVTIRYVDGKP
CCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCC
HSIDTVVLSTQHSPDISLEDLREATIEQIIKPVLPKELIKGDIKYLVNPTGRFVVGGPQG
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCHHEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYVAKNIVAAGLASRCLVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYAIGVAEPTSIMVETYGTGKVSNETLTALVRKHFDLRPKGIVNMLDLLRPIYQKTAAY
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GHFGRDEPEFTWEATDRANLLKSDAGL
CCCCCCCCCEEEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA