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The map label for this gene is metF [H]

Identifier: 71905825

GI number: 71905825

Start: 201469

End: 202293

Strand: Reverse

Name: metF [H]

Synonym: Daro_0183

Alternate gene names: 71905825

Gene position: 202293-201469 (Counterclockwise)

Preceding gene: 71905826

Following gene: 71905821

Centisome position: 4.49

GC content: 60.85

Gene sequence:

>825_bases
ATGAAACCTGAAATCTCCATCGAGTTTTTCCCGCCCCAAACACCGGAAGGCATCGAAAAGCTGCGAACCGTTCGGGCCGA
ACTGGCCAAGCTGAACCCGCATTTCTTCTCGGTCACCTACGGCGCCGGTGGCTCGACCCGCGAACGCACCTTCGCCGTGG
TCAAGGAAATCGCGGCCGAAGGCTTCGACGCGGCACCACACCTGTCATGTATCGGATCGACCCGTGAGTCGATCCGCGAA
ATCCTCGCCGAATACAAGTCCGCCGGCATCAAACGCACCGTCGCCCTGCGCGGCGATCTGCCTTCCGGCATGGCCGAGGC
CGGCGAATTCCGCTACGCCAACGAACTCGTCGAATTCATCCGGGCCGAAACCGGCGACTGGTTCAGCATCGAAGTGGCCG
CCTACCCGGAATGGCATCCGCAGGCCCGTAGTCCAAAGGACGACCTCGAAGCTTTCGCCCGCAAGGTCAAGGCTGGCGCC
AATTCGGCCATCACTCAGTACTTCTACAACGCCGATGCCTACTTCCACTTTGTTGATGAAGCCAAGGCCCTCGGCGTCGA
TGTACCCATCATCCCCGGCATCATGCCGATCGCTGGCTTCACCAAACTGGCCCGCTTCTCGGATGCCTGCGGCGCCGACA
TTCCGCGCTGGATGCGCAAGAAATTTGAGAGTTTCGGCGACGATGCCGACTCCATTCGCGCCTTCGGCCTCGATGTCGTC
ACTGAACTGTGCGAACGCCTGCTCAAAGGCGGGGCCCCCGGTCTGCATTTCTATGCCATGAATCAGAGCGCGCTAACCAC
GGAAATCTGCCGCCGGCTGGGCTAA

Upstream 100 bases:

>100_bases
GAACCTGCGCCGCAAGCCTTGGCCTGAAAGTCCGCGCCTGGCTGGATAGTCCGATTACCGGCGGTGATGGCAACCGCGAA
TTCTTCATCTGGTTGAACAA

Downstream 100 bases:

>100_bases
ATCTGCCTCTCTCCATCGTCGATCAAGCCTGCTTTCGTGGACTTGGTCGACGGTGGAGCTCCACTGAACAACATATATTG
TCAAATCGCTTCTGTTCGTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKPEISIEFFPPQTPEGIEKLRTVRAELAKLNPHFFSVTYGAGGSTRERTFAVVKEIAAEGFDAAPHLSCIGSTRESIRE
ILAEYKSAGIKRTVALRGDLPSGMAEAGEFRYANELVEFIRAETGDWFSIEVAAYPEWHPQARSPKDDLEAFARKVKAGA
NSAITQYFYNADAYFHFVDEAKALGVDVPIIPGIMPIAGFTKLARFSDACGADIPRWMRKKFESFGDDADSIRAFGLDVV
TELCERLLKGGAPGLHFYAMNQSALTTEICRRLG

Sequences:

>Translated_274_residues
MKPEISIEFFPPQTPEGIEKLRTVRAELAKLNPHFFSVTYGAGGSTRERTFAVVKEIAAEGFDAAPHLSCIGSTRESIRE
ILAEYKSAGIKRTVALRGDLPSGMAEAGEFRYANELVEFIRAETGDWFSIEVAAYPEWHPQARSPKDDLEAFARKVKAGA
NSAITQYFYNADAYFHFVDEAKALGVDVPIIPGIMPIAGFTKLARFSDACGADIPRWMRKKFESFGDDADSIRAFGLDVV
TELCERLLKGGAPGLHFYAMNQSALTTEICRRLG
>Mature_274_residues
MKPEISIEFFPPQTPEGIEKLRTVRAELAKLNPHFFSVTYGAGGSTRERTFAVVKEIAAEGFDAAPHLSCIGSTRESIRE
ILAEYKSAGIKRTVALRGDLPSGMAEAGEFRYANELVEFIRAETGDWFSIEVAAYPEWHPQARSPKDDLEAFARKVKAGA
NSAITQYFYNADAYFHFVDEAKALGVDVPIIPGIMPIAGFTKLARFSDACGADIPRWMRKKFESFGDDADSIRAFGLDVV
TELCERLLKGGAPGLHFYAMNQSALTTEICRRLG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=278, Percent_Identity=38.4892086330935, Blast_Score=187, Evalue=7e-48,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=41.5441176470588, Blast_Score=225, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=34.5323741007194, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=34.5323741007194, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=33.8028169014084, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6325234, Length=289, Percent_Identity=32.1799307958478, Blast_Score=153, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24662658, Length=211, Percent_Identity=25.1184834123223, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30242; Mature: 30242

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEISIEFFPPQTPEGIEKLRTVRAELAKLNPHFFSVTYGAGGSTRERTFAVVKEIAAE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
GFDAAPHLSCIGSTRESIREILAEYKSAGIKRTVALRGDLPSGMAEAGEFRYANELVEFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RAETGDWFSIEVAAYPEWHPQARSPKDDLEAFARKVKAGANSAITQYFYNADAYFHFVDE
HHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHH
AKALGVDVPIIPGIMPIAGFTKLARFSDACGADIPRWMRKKFESFGDDADSIRAFGLDVV
HHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
TELCERLLKGGAPGLHFYAMNQSALTTEICRRLG
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MKPEISIEFFPPQTPEGIEKLRTVRAELAKLNPHFFSVTYGAGGSTRERTFAVVKEIAAE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHC
GFDAAPHLSCIGSTRESIREILAEYKSAGIKRTVALRGDLPSGMAEAGEFRYANELVEFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RAETGDWFSIEVAAYPEWHPQARSPKDDLEAFARKVKAGANSAITQYFYNADAYFHFVDE
HHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHH
AKALGVDVPIIPGIMPIAGFTKLARFSDACGADIPRWMRKKFESFGDDADSIRAFGLDVV
HHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
TELCERLLKGGAPGLHFYAMNQSALTTEICRRLG
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]