Click here to switch to the map view.

The map label for this gene is btuF [H]

Identifier: 71905780

GI number: 71905780

Start: 152017

End: 152919

Strand: Reverse

Name: btuF [H]

Synonym: Daro_0138

Alternate gene names: 71905780

Gene position: 152919-152017 (Counterclockwise)

Preceding gene: 71905781

Following gene: 71905779

Centisome position: 3.4

GC content: 62.46

Gene sequence:

>903_bases
ATGGAAACCCGCATGAAAATCCGCTTGCTTTCAGCCTTGGCGACCGCCTTCGTCGTTTCCACCAACGCCCACGCCGACCT
CGTCATCAAGGATGACATCGGCCAGGAAATCCGTCTCAAGGCCCCGGCCAAACGCATCGTTGCCCTCGCCCCCCACATCG
CCGAGAGCCTGTATGCCGCGGGTGCCGGCGACCGGCTGGTCGGTACCGTCGAATACAGCGACTACCCGCCGGAAGCCAAG
AAAGTGCCGCGGGTCGGCGGCTACTCGCGCGTCGACCTTGAGGCCATCGCGGCCCTCAAGCCTGATCTGGTCCTGGCCTG
GGAGAGCGGCAACAACATGCCGCAGGTCGACAAACTGAAGGGGCTTGGCCTGACGGTCTATGTCTCACAGCCAAACAAGA
TCGAAAACGTGGCCGACCAGCTGGTTCGCCTGGGCCAGCTGGCCGGCACCGAGCCCGCCGCCAATGCGGCCGCCGAACGT
TTCCGTAAACGGCTGGACGGCCTGCGCGCTGCCAACGTCGGCAAGCCGAAAGTACGCGTCTTTTATCAAATCTGGAAAAC
CCCGCTGATGACGGTCGGCGGCCCGCAGATCATCAGCGATGCGATCAAGCTATGCGGTGGCGACAATATATTCGGCCACC
TCGCCAAGATGGCGCCAACGGTCAGTGTTGAAGCCGTGCTCGAAGCCGACCCCGAGGCCATCATTGCCACCGGCATGGGC
GACGCCAAGCCGGAATGGCTGCACGACTGGGATAAATGGACACGGATGACGGCGGTCAAACGCGACAACCTGTTCCACAT
CAATCCGGACATCATGCAGCGCCATACGCCGCGCATCCTGGACGGCACCGAGAAACTTTGCGCCCACCTCGATGTCGCAA
GAAGCCACCGCCCCGCCAAATAA

Upstream 100 bases:

>100_bases
AAGGCATCCTGACCCGTCGCTTCGACCAGCAGCCACTACTGCGCTTTGGCCTGCCCTGCAGCGAAGACCAATGGCAACGT
CTGGGCAAGGCGCTTGCCGA

Downstream 100 bases:

>100_bases
AACCATGCCCCGCCTGCCCCAACGCATCGTCTGCCTGACCACCGAAACTGTCGAAGTGCTCTACGCCCTTGGCGAGCAGG
ACCGCATCGTCGGCATTTCC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
METRMKIRLLSALATAFVVSTNAHADLVIKDDIGQEIRLKAPAKRIVALAPHIAESLYAAGAGDRLVGTVEYSDYPPEAK
KVPRVGGYSRVDLEAIAALKPDLVLAWESGNNMPQVDKLKGLGLTVYVSQPNKIENVADQLVRLGQLAGTEPAANAAAER
FRKRLDGLRAANVGKPKVRVFYQIWKTPLMTVGGPQIISDAIKLCGGDNIFGHLAKMAPTVSVEAVLEADPEAIIATGMG
DAKPEWLHDWDKWTRMTAVKRDNLFHINPDIMQRHTPRILDGTEKLCAHLDVARSHRPAK

Sequences:

>Translated_300_residues
METRMKIRLLSALATAFVVSTNAHADLVIKDDIGQEIRLKAPAKRIVALAPHIAESLYAAGAGDRLVGTVEYSDYPPEAK
KVPRVGGYSRVDLEAIAALKPDLVLAWESGNNMPQVDKLKGLGLTVYVSQPNKIENVADQLVRLGQLAGTEPAANAAAER
FRKRLDGLRAANVGKPKVRVFYQIWKTPLMTVGGPQIISDAIKLCGGDNIFGHLAKMAPTVSVEAVLEADPEAIIATGMG
DAKPEWLHDWDKWTRMTAVKRDNLFHINPDIMQRHTPRILDGTEKLCAHLDVARSHRPAK
>Mature_300_residues
METRMKIRLLSALATAFVVSTNAHADLVIKDDIGQEIRLKAPAKRIVALAPHIAESLYAAGAGDRLVGTVEYSDYPPEAK
KVPRVGGYSRVDLEAIAALKPDLVLAWESGNNMPQVDKLKGLGLTVYVSQPNKIENVADQLVRLGQLAGTEPAANAAAER
FRKRLDGLRAANVGKPKVRVFYQIWKTPLMTVGGPQIISDAIKLCGGDNIFGHLAKMAPTVSVEAVLEADPEAIIATGMG
DAKPEWLHDWDKWTRMTAVKRDNLFHINPDIMQRHTPRILDGTEKLCAHLDVARSHRPAK

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=249, Percent_Identity=35.7429718875502, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32783; Mature: 32783

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRMKIRLLSALATAFVVSTNAHADLVIKDDIGQEIRLKAPAKRIVALAPHIAESLYAA
CCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHC
GAGDRLVGTVEYSDYPPEAKKVPRVGGYSRVDLEAIAALKPDLVLAWESGNNMPQVDKLK
CCCCCEEEEEECCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHC
GLGLTVYVSQPNKIENVADQLVRLGQLAGTEPAANAAAERFRKRLDGLRAANVGKPKVRV
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
FYQIWKTPLMTVGGPQIISDAIKLCGGDNIFGHLAKMAPTVSVEAVLEADPEAIIATGMG
HHHHHCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHEECCCCCEEEEECCC
DAKPEWLHDWDKWTRMTAVKRDNLFHINPDIMQRHTPRILDGTEKLCAHLDVARSHRPAK
CCCCHHHHHHHHHHHHHHEECCCEEEECHHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METRMKIRLLSALATAFVVSTNAHADLVIKDDIGQEIRLKAPAKRIVALAPHIAESLYAA
CCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHC
GAGDRLVGTVEYSDYPPEAKKVPRVGGYSRVDLEAIAALKPDLVLAWESGNNMPQVDKLK
CCCCCEEEEEECCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHC
GLGLTVYVSQPNKIENVADQLVRLGQLAGTEPAANAAAERFRKRLDGLRAANVGKPKVRV
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
FYQIWKTPLMTVGGPQIISDAIKLCGGDNIFGHLAKMAPTVSVEAVLEADPEAIIATGMG
HHHHHCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHEECCCCCEEEEECCC
DAKPEWLHDWDKWTRMTAVKRDNLFHINPDIMQRHTPRILDGTEKLCAHLDVARSHRPAK
CCCCHHHHHHHHHHHHHHEECCCEEEECHHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA