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The map label for this gene is pyrE [H]

Identifier: 71905764

GI number: 71905764

Start: 138439

End: 139080

Strand: Reverse

Name: pyrE [H]

Synonym: Daro_0122

Alternate gene names: 71905764

Gene position: 139080-138439 (Counterclockwise)

Preceding gene: 71905770

Following gene: 71905763

Centisome position: 3.09

GC content: 59.03

Gene sequence:

>642_bases
ATGGATTTTCGTCAGGATTTCATCGAATTTGCAGTTGGCTGCCAAGTATTGCGTTTTGGCGAATTCAAAACCAAGGCAGG
AAGGCTTTCACCCTACTTTTTCAATGCCGGACTGTTCAACGACGGCAACTCGCTTGGACGTCTGGCCGAATTCTACGCCA
AAGCAGCAGAAGCCGGCGGCGTCCAGTTCGACATGCTTTTCGGCCCGGCCTACAAGGGCATTCCGCTGGTGGCTGCCATT
GCCATATCGCTTGCTCAGCGCGGCAAGAATTTCCCTTTTGCCTTCAACCGCAAGGAAGCCAAGGACCATGGCGAGGGCGG
CAACATCGTCGGCTCGCCATTGACCGGACGTGTCCTGATCGTCGATGACGTTATTTCCGCCGGAACTTCCGTGCGCGAAT
CAGTCGAACTGATCCGCGCCGCCGGCGCCACCCCGGCAGGCGTATTGATTGCGCTGGATCGCCAGGAGCGCGGACAGGGC
GAACTCTCGGCAGTGCAGGAAGTCCAGCGCGACTATGGCATCCCCGTCGTCGCCGTTGCCGGGCTCAAGGACCTGATGAC
CTTCCTAGCCGAGCGCCCGGATTTTGCCAACCATCGCGAGGCCGTTGCCCACTACCGTGAGCAGTACGGGGTCGATGCCT
AA

Upstream 100 bases:

>100_bases
TTACTCCATGCGGAGCGGATACCATTGAGATTCAGGGAGATGATGCGTAACATTGTGATCGAATCGGTCGTATTTTTCCG
ACCTGCAGGAATAGAGGATT

Downstream 100 bases:

>100_bases
GCGGGCACTCCTTACCCTTCTCCTGTTGCTGGCCACTTCAGCACAGGCAGCCGGAGAGTTCTACTGCTGCCACGATCCGG
TCAGCGGTCGCCGGGTCTGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDFRQDFIEFAVGCQVLRFGEFKTKAGRLSPYFFNAGLFNDGNSLGRLAEFYAKAAEAGGVQFDMLFGPAYKGIPLVAAI
AISLAQRGKNFPFAFNRKEAKDHGEGGNIVGSPLTGRVLIVDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQG
ELSAVQEVQRDYGIPVVAVAGLKDLMTFLAERPDFANHREAVAHYREQYGVDA

Sequences:

>Translated_213_residues
MDFRQDFIEFAVGCQVLRFGEFKTKAGRLSPYFFNAGLFNDGNSLGRLAEFYAKAAEAGGVQFDMLFGPAYKGIPLVAAI
AISLAQRGKNFPFAFNRKEAKDHGEGGNIVGSPLTGRVLIVDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQG
ELSAVQEVQRDYGIPVVAVAGLKDLMTFLAERPDFANHREAVAHYREQYGVDA
>Mature_213_residues
MDFRQDFIEFAVGCQVLRFGEFKTKAGRLSPYFFNAGLFNDGNSLGRLAEFYAKAAEAGGVQFDMLFGPAYKGIPLVAAI
AISLAQRGKNFPFAFNRKEAKDHGEGGNIVGSPLTGRVLIVDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQG
ELSAVQEVQRDYGIPVVAVAGLKDLMTFLAERPDFANHREAVAHYREQYGVDA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1790073, Length=210, Percent_Identity=60.4761904761905, Blast_Score=271, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17508631, Length=161, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=190, Percent_Identity=24.7368421052632, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=221, Percent_Identity=48.868778280543, Blast_Score=208, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323927, Length=219, Percent_Identity=43.37899543379, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23018; Mature: 23018

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRQDFIEFAVGCQVLRFGEFKTKAGRLSPYFFNAGLFNDGNSLGRLAEFYAKAAEAGG
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC
VQFDMLFGPAYKGIPLVAAIAISLAQRGKNFPFAFNRKEAKDHGEGGNIVGSPLTGRVLI
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCEECCCCCCCEEE
VDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQGELSAVQEVQRDYGIPVVAVA
EECHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHH
GLKDLMTFLAERPDFANHREAVAHYREQYGVDA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFRQDFIEFAVGCQVLRFGEFKTKAGRLSPYFFNAGLFNDGNSLGRLAEFYAKAAEAGG
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC
VQFDMLFGPAYKGIPLVAAIAISLAQRGKNFPFAFNRKEAKDHGEGGNIVGSPLTGRVLI
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCEECCCCCCCEEE
VDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQGELSAVQEVQRDYGIPVVAVA
EECHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHH
GLKDLMTFLAERPDFANHREAVAHYREQYGVDA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA