Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 71905698

GI number: 71905698

Start: 65055

End: 65924

Strand: Reverse

Name: purU [H]

Synonym: Daro_0056

Alternate gene names: 71905698

Gene position: 65924-65055 (Counterclockwise)

Preceding gene: 71905699

Following gene: 71905697

Centisome position: 1.46

GC content: 59.08

Gene sequence:

>870_bases
ATGACCATGCATCGCGACCGCTTCTACACGCTTTCCGCTTCCTGCCCCGATCAGGTAGGCATCATCGCCCGCGTTTCCGG
CTTCATCGCCGGCAACGGCGGCTGGATTCTCGAATCGAGTTTCCATTCGGACGTGCTGACCGGTCGCTATTTCATGCGCA
TTGAAATCAAGGCCGATTCGCTGCCCTTCCTGCTCGCCGAATTCCGCGAACGCTTCCGCATTGAAGTCGCCGAACCGCTG
TCGATGACCTGGCAAATCAACGACAGCGCGGTCAAGAAACGCGTTGTCGTGCTGGTTTCCAAGCAGGAGCATTGCCTCTA
TGACCTGCTGGCGCGCTGGCAGGCCAAGGAACTCGACATCGAGATCCCGTGCGTCATTTCCAACCACGACACTTTCCGTG
GCTTTGTCGAATGGCACGGTATTCCGTTCCATCACGTACCGGTGACTGCCGACAACAAGGCCGCCGCCTACGCCGAGATT
CAGCGCATCTTTGAAGACGTGCGCGGCGACTCGATGGTCCTTGCCCGCTACATGCAGGTCTTGTCGCCCGAATTGTGCGA
TGCGCTGACCGGCAAGATCATCAACATCCACCACAGCTTCCTGCCCAGCTTTGCCGGCGCCAAGCCCTACCATCAGGCCT
ACACCCGTGGCGTCAAGCTGATCGGCGCGACCTGCCATTACGTGACCAGCGAACTCGATGCCGGTCCGATCATCGAGCAG
GACGTGATCCGCATCGACCATTCCGATTCGCCCGAAGATATGGTTCGCTACGGCAAGGACATCGAGAAGACGGTACTCGC
TCGTGGCCTGCGCTATCACCTCGAGGATCGCGTCCTCGTTCACGGCAACAAGACGGTCGTTTTCCGCTGA

Upstream 100 bases:

>100_bases
AAAACGTCATTCGCGAATTCCCGCAGTACCCGGTCGTGCTGAACTACGACGATCTGCGCAGCGAAATCGACAAGGCTTTC
GCCAAGGCAGCCTGAGCCCA

Downstream 100 bases:

>100_bases
TGCTGATTCGACGTGACGATTCGCTGCTGCTGATCGTCGACATTCAGGAAAAATTGGCGCCGGCCATTCACGACAACGAG
CGCGTTACGGCCAATTCGGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTMHRDRFYTLSASCPDQVGIIARVSGFIAGNGGWILESSFHSDVLTGRYFMRIEIKADSLPFLLAEFRERFRIEVAEPL
SMTWQINDSAVKKRVVVLVSKQEHCLYDLLARWQAKELDIEIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKAAAYAEI
QRIFEDVRGDSMVLARYMQVLSPELCDALTGKIINIHHSFLPSFAGAKPYHQAYTRGVKLIGATCHYVTSELDAGPIIEQ
DVIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRVLVHGNKTVVFR

Sequences:

>Translated_289_residues
MTMHRDRFYTLSASCPDQVGIIARVSGFIAGNGGWILESSFHSDVLTGRYFMRIEIKADSLPFLLAEFRERFRIEVAEPL
SMTWQINDSAVKKRVVVLVSKQEHCLYDLLARWQAKELDIEIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKAAAYAEI
QRIFEDVRGDSMVLARYMQVLSPELCDALTGKIINIHHSFLPSFAGAKPYHQAYTRGVKLIGATCHYVTSELDAGPIIEQ
DVIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRVLVHGNKTVVFR
>Mature_288_residues
TMHRDRFYTLSASCPDQVGIIARVSGFIAGNGGWILESSFHSDVLTGRYFMRIEIKADSLPFLLAEFRERFRIEVAEPLS
MTWQINDSAVKKRVVVLVSKQEHCLYDLLARWQAKELDIEIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKAAAYAEIQ
RIFEDVRGDSMVLARYMQVLSPELCDALTGKIINIHHSFLPSFAGAKPYHQAYTRGVKLIGATCHYVTSELDAGPIIEQD
VIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRVLVHGNKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869995, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869993, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787483, Length=289, Percent_Identity=44.636678200692, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=27.4611398963731, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=161, Percent_Identity=31.6770186335404, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582400, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32914; Mature: 32783

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMHRDRFYTLSASCPDQVGIIARVSGFIAGNGGWILESSFHSDVLTGRYFMRIEIKADS
CCCCCCEEEEEECCCCCHHCHHEEEEEEEECCCCEEEECCCCCCHHCCEEEEEEEEECCC
LPFLLAEFRERFRIEVAEPLSMTWQINDSAVKKRVVVLVSKQEHCLYDLLARWQAKELDI
CCHHHHHHHHHHEEEEECCCEEEEEECHHHHCEEEEEEEECCCHHHHHHHHHCCCCCCCE
EIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKAAAYAEIQRIFEDVRGDSMVLARYMQV
EEEEEECCCCHHHHEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSPELCDALTGKIINIHHSFLPSFAGAKPYHQAYTRGVKLIGATCHYVTSELDAGPIIEQ
HCHHHHHHHHCCEEEEHHHHCCHHCCCCHHHHHHHCCHHEEEHHHHHHHHCCCCCCCCCC
DVIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRVLVHGNKTVVFR
CEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC
>Mature Secondary Structure 
TMHRDRFYTLSASCPDQVGIIARVSGFIAGNGGWILESSFHSDVLTGRYFMRIEIKADS
CCCCCEEEEEECCCCCHHCHHEEEEEEEECCCCEEEECCCCCCHHCCEEEEEEEEECCC
LPFLLAEFRERFRIEVAEPLSMTWQINDSAVKKRVVVLVSKQEHCLYDLLARWQAKELDI
CCHHHHHHHHHHEEEEECCCEEEEEECHHHHCEEEEEEEECCCHHHHHHHHHCCCCCCCE
EIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKAAAYAEIQRIFEDVRGDSMVLARYMQV
EEEEEECCCCHHHHEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSPELCDALTGKIINIHHSFLPSFAGAKPYHQAYTRGVKLIGATCHYVTSELDAGPIIEQ
HCHHHHHHHHCCEEEEHHHHCCHHCCCCHHHHHHHCCHHEEEHHHHHHHHCCCCCCCCCC
DVIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRVLVHGNKTVVFR
CEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]