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The map label for this gene is ksgA [H]

Identifier: 71894000

GI number: 71894000

Start: 854060

End: 854812

Strand: Reverse

Name: ksgA [H]

Synonym: MHJ_0652

Alternate gene names: 71894000

Gene position: 854812-854060 (Counterclockwise)

Preceding gene: 71894001

Following gene: 71893999

Centisome position: 95.25

GC content: 25.63

Gene sequence:

>753_bases
TTAGGTCAGAATTTTTTAAAAGATAGAAAAATTGCTGAAAAAATAGTTGAAAATATTGATCTTAAAAACAAAGAAATTAT
CGAAATAGGTTGTGGAACCGGTTTTTTAACAAATTTTTTACTTGAAAAAGCAAAATTTGTTACTTGTTATGAAATTGATA
GAAATTTAATTCCAATTCTTGAAAAAAAATTCAAGAATAAAAATCTTCGTATAATTAACGAAGATTTTTTGCTCGCAGAA
CTCGAATTTAAAGAAAAAAAAACAATTATAGCCAACCTTCCTTACTATATTACCTCAAAAATTTTGTTTAAAATCTTTGC
AAATTTTGAAAAATTTGATAAAATTATCTTAATGGTTCAAAATGAGGTCGCCGATCGAATAGTTGCCAAACCAAAAACAC
CAACTTATTCAAAATTATCGCTTGCATCACAATATATAGCGCATGTCAAAAAATTGTTTGTAGTTGGACCGGATTCGTTT
TTTCCAAAGCCGAAAATCAATTCAGCTGTTGTTTTTTTTGATCTTAGAACAAACTTGGATGCCAAAAAAACGGAGCAATT
TTTTTGGTTTACAAAAAAATGCTTTCAATTTAAACGAAAAACTCTATATAATAATCTTATTTTTTTTCTAAATAAGCAGC
AAATTGAAAAAATTTATAATTTTTTTCAATTTGCTCAAAATATAAGACCTCAACAGCTTGATTTGGTAACTTATATAAGG
TTAGCCGACTTTTATTTTAACAATATTTTTTAA

Upstream 100 bases:

>100_bases
TTAAAAAATTTGACAAAAACAGAACTTTCTGAAATAATTTATAGAAATGTAAGGAAAATTTTTAAAGTAAAAAATGCAAA
AACCGGTTCCTAAGAAGCAT

Downstream 100 bases:

>100_bases
AAAAAATATTTTGTTTTTTTAAAAAATATTGTATAATTTTAATACATTCTTGACAACCAAAAATTTGGTTGCTTATTTAT
TATTTTTTCTTATTTTTTTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDRNLIPILEKKFKNKNLRIINEDFLLAE
LEFKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAHVKKLFVVGPDSF
FPKPKINSAVVFFDLRTNLDAKKTEQFFWFTKKCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQQLDLVTYIR
LADFYFNNIF

Sequences:

>Translated_250_residues
LGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDRNLIPILEKKFKNKNLRIINEDFLLAE
LEFKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAHVKKLFVVGPDSF
FPKPKINSAVVFFDLRTNLDAKKTEQFFWFTKKCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQQLDLVTYIR
LADFYFNNIF
>Mature_250_residues
LGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDRNLIPILEKKFKNKNLRIINEDFLLAE
LEFKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAHVKKLFVVGPDSF
FPKPKINSAVVFFDLRTNLDAKKTEQFFWFTKKCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQQLDLVTYIR
LADFYFNNIF

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=233, Percent_Identity=33.9055793991416, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI156415992, Length=278, Percent_Identity=29.8561151079137, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=28.4046692607004, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=35.2657004830918, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=241, Percent_Identity=28.6307053941909, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=181, Percent_Identity=34.2541436464088, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=33.7662337662338, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 29702; Mature: 29702

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
LGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDRNLIPIL
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEECCCCHHHHH
EKKFKNKNLRIINEDFLLAELEFKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQ
HHHHCCCCCEEEECCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHH
NEVADRIVAKPKTPTYSKLSLASQYIAHVKKLFVVGPDSFFPKPKINSAVVFFDLRTNLD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCCCC
AKKTEQFFWFTKKCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQQLDLVTYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LADFYFNNIF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA