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The map label for this gene is rlmB [H]

Identifier: 71893988

GI number: 71893988

Start: 839821

End: 840522

Strand: Reverse

Name: rlmB [H]

Synonym: MHJ_0640

Alternate gene names: 71893988

Gene position: 840522-839821 (Counterclockwise)

Preceding gene: 71893989

Following gene: 71893987

Centisome position: 93.66

GC content: 28.63

Gene sequence:

>702_bases
ATGGTTAAATATATTTGTGGCAGAAATTCATTAGCCGACGCGATCAAGAATGGGTTTATTTTTAAGGAAATTTACCTTTT
AAAACCTACAAATGATCAAATTTTCTTAAAACAAAAAGTAAAAATAGTTAGTCGTGATTTTTTAGATAAACTTGTCAGAC
TGAATCACCAAGGTTTTGTTGGTGTTCTTGAGAATTTTAAATTTTTTGAAATTGATACATTAGTAAAAGATAAACCAAAA
ATTATTTTGATCCTTGATCATATCCAGGACCAAAATAATCTTGGAAATATCATTAGAACTGCAAATTGTTTTGGAATTAA
GCATATAATTTTACCTAAAAGGCGAGCAGCAAAATTGAATGAGACCACTATGAAAGTCTCATCTGGTGGTTTTGTCGGCC
TTAAATTTATTTTAGTTAATTCCATTGTTTCAGCAATTAATAAATTAAAAGAGATGGATTTTTGAATTTATGCCACTGAT
CTTAAAGAAAAAAGTAGAAAAATCCAGGAAATTCAGTTTAATTATCCTTGCGCAATAATTTTAGGTAATGAGGGAACTGG
AATCAAAAAAAGCAGTTTAAATGCAAGTGATCAAACTTTTTTTATCCCGCTTGAAGGAACAGTTAACTCGCTAAATGTGA
CAGTCGCGACCGGAATTATTCTTTTTTTTCTAAAACAAAATATAAAAAAAACAAAAATATAA

Upstream 100 bases:

>100_bases
GGATAAAAACTATTCCGAGGCCGATAAAATCCGTGAAGAATTATGAAAAATATTTAAAAATTCATAATTTTTTCCATCTT
CCTTTTGAAAGATTGAAAGT

Downstream 100 bases:

>100_bases
AGGGAAAAAATGTGTTTTCTATGTCACCAGAGCGAAAAGAAAAGATAAAAAAAAATAAACGCTGTTTAAAGGTTTTAAAT
AAATATTCACATATTTTATT

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLKPTNDQIFLKQKVKIVSRDFLDKLVRLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKRRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFWIYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI

Sequences:

>Translated_233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLKPTNDQIFLKQKVKIVSRDFLDKLVRLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKRRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDF*IYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
>Mature_233_residues
MVKYICGRNSLADAIKNGFIFKEIYLLKPTNDQIFLKQKVKIVSRDFLDKLVRLNHQGFVGVLENFKFFEIDTLVKDKPK
IILILDHIQDQNNLGNIIRTANCFGIKHIILPKRRAAKLNETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDF*IYATD
LKEKSRKIQEIQFNYPCAIILGNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=30.0653594771242, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26251; Mature: 26251

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYICGRNSLADAIKNGFIFKEIYLLKPTNDQIFLKQKVKIVSRDFLDKLVRLNHQGFV
CEEEEECCCHHHHHHHCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
GVLENFKFFEIDTLVKDKPKIILILDHIQDQNNLGNIIRTANCFGIKHIILPKRRAAKLN
HHHCCCEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHCCCC
ETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFIYATDLKEKSRKIQEIQFNYPCAIIL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHEEEECCCEEEEE
GNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKYICGRNSLADAIKNGFIFKEIYLLKPTNDQIFLKQKVKIVSRDFLDKLVRLNHQGFV
CEEEEECCCHHHHHHHCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
GVLENFKFFEIDTLVKDKPKIILILDHIQDQNNLGNIIRTANCFGIKHIILPKRRAAKLN
HHHCCCEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHCCCC
ETTMKVSSGGFVGLKFILVNSIVSAINKLKEMDFIYATDLKEKSRKIQEIQFNYPCAIIL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHEEEECCCEEEEE
GNEGTGIKKSSLNASDQTFFIPLEGTVNSLNVTVATGIILFFLKQNIKKTKI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA