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The map label for this gene is fusA [H]

Identifier: 71893430

GI number: 71893430

Start: 89995

End: 92082

Strand: Reverse

Name: fusA [H]

Synonym: MHJ_0071

Alternate gene names: 71893430

Gene position: 92082-89995 (Counterclockwise)

Preceding gene: 71893431

Following gene: 71893421

Centisome position: 10.26

GC content: 37.07

Gene sequence:

>2088_bases
ATTATGGCACGAAAATTTGAACTAAAAGATTATCGCAATATTGGAATTATGGCCCATATTGATGCAGGGAAAACAACAAC
AACTGAAAGAATTCTATTCCATACAGGAAAAATTCATAAAATTGGCGAAACTCATGATGGGGTTAGCCAAATGGACTGAA
TGGAACAGGAAAAAGAACGCGGAATTACGATAACTTCGGCAGCAACAACTGCTTTTTGAAAAGGAAAAAGAATTAACATT
ATCGATACACCAGGCCATGTTGATTTCACTGTCGAAGTTGAAAGATCATTACGGGTTTTAGATGGGGCAGTTGCTGTTTT
AGATGCCCAATCCGGGGTTGAACCTCAGACTGAAACTGTTTGAAGACAAGCAACAAATTATAGCGTTCCAAGAATTGTTT
ATGTCAATAAAATGGATAAAGCTGGTGCAAATTTTGAAGCTTCAATTGAATCAGTGCGGACAAAATTAAACGGGAATGCA
GTTGCAATTCAGTTAAATATTGGCGCTGAAGCCGATTTTAGTGGACTTATCGATCTTGTTGAAATGAAGGCTTATAATTA
TGATGGCCAAAAAGAAGAAATCGAATATGAAATTCCAATTCCTGAAGATCTTTTCGAAAAAGCAAGTCAAATGCGACTTG
CACTTGCAGAAGCAGTTGCTGACTATGATGAAGAAATTTTTAATAATTTATTAGAAGAAAAGGAAATTTTACCTGAACAA
TTAAAAGCTGCAATTCGGGCTGCAACAATTACAGGTAATTTTTTCCCTGTTGTCTGCGGATCTTCCTTTAAAAACAAGGG
TGTTAAAAAGATGATCGATGCGGTTATCGATTATTTACCTTCCCCTGTTGATGTTCCCCCAATTAAAGCGTTTCGTGATG
AGGAAGAAATTACAATTGAAGCTAGCGATGATCAAGAATTTTCTGCCCTTGCTTTTAAAATTATGAATGATCCTTTTGTT
GGATCACTTACTTTTTTCCGGGTTTATTCTGGAGTTTTAAAAAAAGGTACTTATATAATTAACTCAACAAAAGGCAAAAA
AGAACGTGTAGGCCGTATTTTAGCTATGCATGCTAATTCCCGTGAAGAAATTGATGAGGTAAGAACTGGTGATATTGGTG
CCTTTGTTGGTCTAAAAGATACAACAACTGGTGATTCTTTAATTTCCGAAAAAGCAAAAACTTTTGTTCTTGAAAGGATG
AATTTTCCTGAACCTGTGATTTCCCAGTCACTTGAACCTTTTTCAAAAGCCGAAATTGAAAAATTAGCTACTGCCCTCCA
AAAATTAGCAAATGAAGATCCAACTTTCAAAACTTGAACTGATATTGAAACCGGACAGACAATAATTGCCGGAATGGGCG
AATTGCACCTTGATATTATTGTTGATCGGCTCAAAAGAGAGTTTAATGTTCAAGCTCGTGTAGGAAAACCGCAGGTTTCT
TATCGCGAGACAATTACAAAAAGCGCTGAAGTTGAAGGAAAATATATCAAACAATCCGGTGGTCGTGGGCAGTACGGTCA
TGTTTGGATTAAATTTGAACCTAATCCTGAAGAAGGTTTTGATTTTATTGATAAAATTGTTGGGGGGAAAATTCCAAAAG
AATATATCAAATCAATTCAAAAAGGACTAGAAGAAAAAATGCAAGCCGGAATTCTTGCTGGCTATCCTTTAATTAATTTA
AGGGCAACTTTATTTGATGGTTCTTTTCATGAAGTCGATTCTTCTGAAATGGCCTTTAAAATCGCAGCATCTAAGGCACT
TTCCCGCGCCAGAGATGCTGTTGGAACAGTACTTTTAGAGCCGATTATGGATGTTTCTGTTTTTGCTCCCTCTGAATATG
CAGGAGATGTAATGGGAGATTTATCACGTCGTCGTGGTCTAGTTCGTGAACAGGAAACTCGTTCTGATGGGGCAAATGTA
ATTCGCGGACACGTCCCACTTGCAGAAATGTTCGGGTATTCAACGCAATTACGTTCAATGACTTCAGGCCGGGGAACCTA
TCAGATGCAATTTAATCACTATGAAATTGTACCCAAAAATATTTCCGATGTCATCGTAAAACAAAGAGCAATTAAGGAAG
ATGACTAA

Upstream 100 bases:

>100_bases
TTATAATAAAACCGGAGGGGCCTTTAAGAAAAAAGAAGATACCCACAAAATGGCGGAAGCAAATAGGGCTTTTGCCCACT
TTAAATGGTAGGAATTTAGA

Downstream 100 bases:

>100_bases
AAAAAGAAAACTATAAAAAAATATCGAAAAATTAAGAAAACCGAGTTTTTAGATGAATTAATTTCATTTTTTTCTAAAAA
CTCGGTTTTTTATACAATTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MMARKFELKDYRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHDGVSQMDWMEQEKERGITITSAATTAFWKGKRINI
IDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATNYSVPRIVYVNKMDKAGANFEASIESVRTKLNGNA
VAIQLNIGAEADFSGLIDLVEMKAYNYDGQKEEIEYEIPIPEDLFEKASQMRLALAEAVADYDEEIFNNLLEEKEILPEQ
LKAAIRAATITGNFFPVVCGSSFKNKGVKKMIDAVIDYLPSPVDVPPIKAFRDEEEITIEASDDQEFSALAFKIMNDPFV
GSLTFFRVYSGVLKKGTYIINSTKGKKERVGRILAMHANSREEIDEVRTGDIGAFVGLKDTTTGDSLISEKAKTFVLERM
NFPEPVISQSLEPFSKAEIEKLATALQKLANEDPTFKTWTDIETGQTIIAGMGELHLDIIVDRLKREFNVQARVGKPQVS
YRETITKSAEVEGKYIKQSGGRGQYGHVWIKFEPNPEEGFDFIDKIVGGKIPKEYIKSIQKGLEEKMQAGILAGYPLINL
RATLFDGSFHEVDSSEMAFKIAASKALSRARDAVGTVLLEPIMDVSVFAPSEYAGDVMGDLSRRRGLVREQETRSDGANV
IRGHVPLAEMFGYSTQLRSMTSGRGTYQMQFNHYEIVPKNISDVIVKQRAIKEDD

Sequences:

>Translated_695_residues
MMARKFELKDYRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHDGVSQMD*MEQEKERGITITSAATTAF*KGKRINI
IDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETV*RQATNYSVPRIVYVNKMDKAGANFEASIESVRTKLNGNA
VAIQLNIGAEADFSGLIDLVEMKAYNYDGQKEEIEYEIPIPEDLFEKASQMRLALAEAVADYDEEIFNNLLEEKEILPEQ
LKAAIRAATITGNFFPVVCGSSFKNKGVKKMIDAVIDYLPSPVDVPPIKAFRDEEEITIEASDDQEFSALAFKIMNDPFV
GSLTFFRVYSGVLKKGTYIINSTKGKKERVGRILAMHANSREEIDEVRTGDIGAFVGLKDTTTGDSLISEKAKTFVLERM
NFPEPVISQSLEPFSKAEIEKLATALQKLANEDPTFKT*TDIETGQTIIAGMGELHLDIIVDRLKREFNVQARVGKPQVS
YRETITKSAEVEGKYIKQSGGRGQYGHVWIKFEPNPEEGFDFIDKIVGGKIPKEYIKSIQKGLEEKMQAGILAGYPLINL
RATLFDGSFHEVDSSEMAFKIAASKALSRARDAVGTVLLEPIMDVSVFAPSEYAGDVMGDLSRRRGLVREQETRSDGANV
IRGHVPLAEMFGYSTQLRSMTSGRGTYQMQFNHYEIVPKNISDVIVKQRAIKEDD
>Mature_695_residues
MMARKFELKDYRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHDGVSQMD*MEQEKERGITITSAATTAF*KGKRINI
IDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETV*RQATNYSVPRIVYVNKMDKAGANFEASIESVRTKLNGNA
VAIQLNIGAEADFSGLIDLVEMKAYNYDGQKEEIEYEIPIPEDLFEKASQMRLALAEAVADYDEEIFNNLLEEKEILPEQ
LKAAIRAATITGNFFPVVCGSSFKNKGVKKMIDAVIDYLPSPVDVPPIKAFRDEEEITIEASDDQEFSALAFKIMNDPFV
GSLTFFRVYSGVLKKGTYIINSTKGKKERVGRILAMHANSREEIDEVRTGDIGAFVGLKDTTTGDSLISEKAKTFVLERM
NFPEPVISQSLEPFSKAEIEKLATALQKLANEDPTFKT*TDIETGQTIIAGMGELHLDIIVDRLKREFNVQARVGKPQVS
YRETITKSAEVEGKYIKQSGGRGQYGHVWIKFEPNPEEGFDFIDKIVGGKIPKEYIKSIQKGLEEKMQAGILAGYPLINL
RATLFDGSFHEVDSSEMAFKIAASKALSRARDAVGTVLLEPIMDVSVFAPSEYAGDVMGDLSRRRGLVREQETRSDGANV
IRGHVPLAEMFGYSTQLRSMTSGRGTYQMQFNHYEIVPKNISDVIVKQRAIKEDD

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=691, Percent_Identity=44.1389290882779, Blast_Score=532, Evalue=1e-151,
Organism=Homo sapiens, GI19923640, Length=720, Percent_Identity=39.4444444444444, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI25306283, Length=439, Percent_Identity=43.9635535307517, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI25306287, Length=283, Percent_Identity=52.2968197879859, Blast_Score=252, Evalue=8e-67,
Organism=Homo sapiens, GI4503483, Length=422, Percent_Identity=25.8293838862559, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI157426893, Length=175, Percent_Identity=34.8571428571429, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=39.2592592592593, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=38.9830508474576, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=38.9830508474576, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=38.9830508474576, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI1789738, Length=706, Percent_Identity=56.9405099150142, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=475, Percent_Identity=27.5789473684211, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=42.7586206896552, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=149, Percent_Identity=38.9261744966443, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=676, Percent_Identity=40.6804733727811, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=716, Percent_Identity=30.1675977653631, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17506493, Length=488, Percent_Identity=25.8196721311475, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=40.4109589041096, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=32.8467153284672, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17506081, Length=132, Percent_Identity=32.5757575757576, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=32.8467153284672, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=674, Percent_Identity=42.5816023738872, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6322359, Length=787, Percent_Identity=31.5120711562897, Blast_Score=341, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324707, Length=484, Percent_Identity=25, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=484, Percent_Identity=25, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=35.6643356643357, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=689, Percent_Identity=41.799709724238, Blast_Score=524, Evalue=1e-148,
Organism=Drosophila melanogaster, GI221458488, Length=706, Percent_Identity=33.28611898017, Blast_Score=336, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24585709, Length=429, Percent_Identity=25.4079254079254, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585711, Length=429, Percent_Identity=25.4079254079254, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585713, Length=429, Percent_Identity=25.4079254079254, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI78706572, Length=141, Percent_Identity=39.7163120567376, Blast_Score=94, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=35.5072463768116, Blast_Score=76, Evalue=8e-14,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76723; Mature: 76723

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARKFELKDYRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHDGVSQMDMEQEKERG
CCCCCCCCCCCCCCCEEEEECCCCCCCHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCC
ITITSAATTAFKGKRINIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVRQ
EEEEECHHHHCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHEEEEECCCCCCCHHHHHHH
ATNYSVPRIVYVNKMDKAGANFEASIESVRTKLNGNAVAIQLNIGAEADFSGLIDLVEMK
HHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
AYNYDGQKEEIEYEIPIPEDLFEKASQMRLALAEAVADYDEEIFNNLLEEKEILPEQLKA
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AIRAATITGNFFPVVCGSSFKNKGVKKMIDAVIDYLPSPVDVPPIKAFRDEEEITIEASD
HHHHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEECC
DQEFSALAFKIMNDPFVGSLTFFRVYSGVLKKGTYIINSTKGKKERVGRILAMHANSREE
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHCEEEEEECCCHHH
IDEVRTGDIGAFVGLKDTTTGDSLISEKAKTFVLERMNFPEPVISQSLEPFSKAEIEKLA
HHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHH
TALQKLANEDPTFKTTDIETGQTIIAGMGELHLDIIVDRLKREFNVQARVGKPQVSYRET
HHHHHHCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
ITKSAEVEGKYIKQSGGRGQYGHVWIKFEPNPEEGFDFIDKIVGGKIPKEYIKSIQKGLE
HHHCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
EKMQAGILAGYPLINLRATLFDGSFHEVDSSEMAFKIAASKALSRARDAVGTVLLEPIMD
HHHHCCEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VSVFAPSEYAGDVMGDLSRRRGLVREQETRSDGANVIRGHVPLAEMFGYSTQLRSMTSGR
CEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCHHHHHCCHHHHHHHHCCC
GTYQMQFNHYEIVPKNISDVIVKQRAIKEDD
CEEEEEECEEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMARKFELKDYRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHDGVSQMDMEQEKERG
CCCCCCCCCCCCCCCEEEEECCCCCCCHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCC
ITITSAATTAFKGKRINIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVRQ
EEEEECHHHHCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHEEEEECCCCCCCHHHHHHH
ATNYSVPRIVYVNKMDKAGANFEASIESVRTKLNGNAVAIQLNIGAEADFSGLIDLVEMK
HHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
AYNYDGQKEEIEYEIPIPEDLFEKASQMRLALAEAVADYDEEIFNNLLEEKEILPEQLKA
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AIRAATITGNFFPVVCGSSFKNKGVKKMIDAVIDYLPSPVDVPPIKAFRDEEEITIEASD
HHHHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEECC
DQEFSALAFKIMNDPFVGSLTFFRVYSGVLKKGTYIINSTKGKKERVGRILAMHANSREE
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHCEEEEEECCCHHH
IDEVRTGDIGAFVGLKDTTTGDSLISEKAKTFVLERMNFPEPVISQSLEPFSKAEIEKLA
HHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHH
TALQKLANEDPTFKTTDIETGQTIIAGMGELHLDIIVDRLKREFNVQARVGKPQVSYRET
HHHHHHCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
ITKSAEVEGKYIKQSGGRGQYGHVWIKFEPNPEEGFDFIDKIVGGKIPKEYIKSIQKGLE
HHHCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
EKMQAGILAGYPLINLRATLFDGSFHEVDSSEMAFKIAASKALSRARDAVGTVLLEPIMD
HHHHCCEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VSVFAPSEYAGDVMGDLSRRRGLVREQETRSDGANVIRGHVPLAEMFGYSTQLRSMTSGR
CEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCHHHHHCCHHHHHHHHCCC
GTYQMQFNHYEIVPKNISDVIVKQRAIKEDD
CEEEEEECEEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA