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The map label for this gene is cls [H]

Identifier: 71893393

GI number: 71893393

Start: 44054

End: 45592

Strand: Reverse

Name: cls [H]

Synonym: MHJ_0034

Alternate gene names: 71893393

Gene position: 45592-44054 (Counterclockwise)

Preceding gene: 71893394

Following gene: 71893392

Centisome position: 5.08

GC content: 23.78

Gene sequence:

>1539_bases
ATTATTATTATAATGCATAAATTTATAAAATGATTTTTTTACTATTGTTTCTTAGTAATATTTTTAGTTGCTTTTAGTGC
CACAATTTATTTAATTCATTATTTTTTAGCAAAGAATATGAATTGAATTTCAATTTTGACTATTTTTATAGTTTATGCTT
CTACCTCTTTTTTTAATTTATTTATATTATTACAAAAAAGAAGGTATGAAACGAAAATTTCTTGATTAATTGCTTGTTCA
ATTTTGCCAATAATAGGCCCAATTTCATATATTTTTTTAGGCAGAAAATACTTAAAAAGTCAAAATATTAAAAAATATTT
TGCACAATACAGAAATTTTACTAACCTAAAAGAAAAAGTTCAAACCTGGAAAAATCAAGAATCTAAAGATACTTTACTAA
GTTTTTCTAGTGAATATTTTTCATCCCCAATTAAAGAATTTAATGGAAATTTGCTAGTTGATGGTCATAGTTTTTTTGAA
AAACTTTTTGCTGACATTAAAAATGCAAAAAAATTTATTTTTATTGATGTTTATATAATAAAAAATGATTTTATTTGAAA
AAAATTAAAAAAAATACTGGTTGATAAGCGGAAATGCGGGGTGATAATTAAAATTTTAGTCGATTCTTTTGGCACCTATC
TTATAAAAAGGCGCCATTGAATCGAATTAAAGAAGCAAAAAATTGAGGTTTTGCACTTTAACACCTTTAAAATTCCATTT
GTTTCCGGACAAAGTTTTTACCGAAATCACCGAAAAGTTTATATTATTGACGGAAAAATTGCTTATACAGGCGGAAATAA
TATATCCGAAGAATATTCAAGTTTTGATAAAAATTATGGATATTGAATGGATTTAAACCTTAGACTTGAAGGTGAAATTG
TTCAAACTTACTGTAAAAATTTTCTGTTCCATTGAACAAAATGAGGCAAAAAAAAGATATCAAAAGAAAAAATCAATGGA
TTTTGCAGTATTGAAAATTCTAATCTAAAGCAAGACAAAAACGATAATCTTGGTGTAGTTATTCAAAATGGACCCAACCT
CGAGCACTCATTAATTGAGGGTTTTATTTTAAAAAAAATTTATTCAGCAAAAAAAAATATAAAAATTTTTACCCCTTATC
TTGTTCCCTCGCAAAAAATTATTGATGCTTTAAAGGATATTTTGCTTGCTAAAATTGAGGTTAATTTTTTCTTACCGGGG
AGAAATGATTCAAAAATTATTAAAACTTTCAATGATTTTTTTGCTAAAAAATTATTGAAATATGGAGCAAAAATACACTA
TTTTAAAGAACTTTTCTTCCATGGAAAGTCAATAATCATTGATGATAAATATGGAATGATAGGAACCTCGAATTTAGATT
ATCGATCGCTTCTTTTTCAATATGAAACTAATTTATTTTTTAAAGGAAAAATTTTAGGTGACTTTTTAAATTATATTCAG
GTACTAAAAAATCAAAATATTATTATTGAAATTAATAAGGTATCAAAAGTTTTTTTTATCCTTCGTTTTTTTATATTTTT
TTTAAAAACTGTTATATAA

Upstream 100 bases:

>100_bases
TTACTACTTGAAAATAATACCAAACTATTTAATGAAATAAAGGATCAAATTATTGAAAAACTAAAGGAAAATCAACAACA
ATCTCAAACATTATAGTAAA

Downstream 100 bases:

>100_bases
AACTGTCTAATTTATAGTATAATTATTAATATTATTATTGGTGTTTTTGAATTATGTGAAGAACAATCATCGTTACGTTT
ATTGCCATTTTTGGTGTTTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIIIMHKFIKWFFYYCFLVIFLVAFSATIYLIHYFLAKNMNWISILTIFIVYASTSFFNLFILLQKRRYETKISWLIACS
ILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFE
KLFADIKNAKKFIFIDVYIIKNDFIWKKLKKILVDKRKCGVIIKILVDSFGTYLIKRRHWIELKKQKIEVLHFNTFKIPF
VSGQSFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYWMDLNLRLEGEIVQTYCKNFLFHWTKWGKKKISKEKING
FCSIENSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPG
RNDSKIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILGDFLNYIQ
VLKNQNIIIEINKVSKVFFILRFFIFFLKTVI

Sequences:

>Translated_512_residues
MIIIMHKFIK*FFYYCFLVIFLVAFSATIYLIHYFLAKNMN*ISILTIFIVYASTSFFNLFILLQKRRYETKIS*LIACS
ILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFE
KLFADIKNAKKFIFIDVYIIKNDFI*KKLKKILVDKRKCGVIIKILVDSFGTYLIKRRH*IELKKQKIEVLHFNTFKIPF
VSGQSFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGY*MDLNLRLEGEIVQTYCKNFLFH*TK*GKKKISKEKING
FCSIENSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPG
RNDSKIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILGDFLNYIQ
VLKNQNIIIEINKVSKVFFILRFFIFFLKTVI
>Mature_512_residues
MIIIMHKFIK*FFYYCFLVIFLVAFSATIYLIHYFLAKNMN*ISILTIFIVYASTSFFNLFILLQKRRYETKIS*LIACS
ILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFE
KLFADIKNAKKFIFIDVYIIKNDFI*KKLKKILVDKRKCGVIIKILVDSFGTYLIKRRH*IELKKQKIEVLHFNTFKIPF
VSGQSFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGY*MDLNLRLEGEIVQTYCKNFLFH*TK*GKKKISKEKING
FCSIENSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPG
RNDSKIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILGDFLNYIQ
VLKNQNIIIEINKVSKVFFILRFFIFFLKTVI

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=456, Percent_Identity=25.6578947368421, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787007, Length=366, Percent_Identity=24.8633879781421, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59182; Mature: 59182

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIMHKFIKFFYYCFLVIFLVAFSATIYLIHYFLAKNMNISILTIFIVYASTSFFNLFI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLQKRRYETKISLIACSILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
KNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFADIKNAKKFIFIDVYIIKND
CCCCCHHHHHHHHHHHHHCCHHHCCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEEECHH
FIKKLKKILVDKRKCGVIIKILVDSFGTYLIKRRHIELKKQKIEVLHFNTFKIPFVSGQS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEEEECCHH
FYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYMDLNLRLEGEIVQTYCKNFLFHTK
HHCCCCEEEEEECEEEEECCCCCHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHEECC
GKKKISKEKINGFCSIENSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIK
CHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCE
IFTPYLVPSQKIIDALKDILLAKIEVNFFLPGRNDSKIIKTFNDFFAKKLLKYGAKIHYF
EEECCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
KELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILGDFLNYIQVLKNQNII
HHHHHCCCEEEEECCCCCEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEE
IEINKVSKVFFILRFFIFFLKTVI
EEEHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIIIMHKFIKFFYYCFLVIFLVAFSATIYLIHYFLAKNMNISILTIFIVYASTSFFNLFI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLQKRRYETKISLIACSILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
KNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFADIKNAKKFIFIDVYIIKND
CCCCCHHHHHHHHHHHHHCCHHHCCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEEECHH
FIKKLKKILVDKRKCGVIIKILVDSFGTYLIKRRHIELKKQKIEVLHFNTFKIPFVSGQS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEEEECCHH
FYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYMDLNLRLEGEIVQTYCKNFLFHTK
HHCCCCEEEEEECEEEEECCCCCHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHEECC
GKKKISKEKINGFCSIENSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIK
CHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCE
IFTPYLVPSQKIIDALKDILLAKIEVNFFLPGRNDSKIIKTFNDFFAKKLLKYGAKIHYF
EEECCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
KELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILGDFLNYIQVLKNQNII
HHHHHCCCEEEEECCCCCEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEE
IEINKVSKVFFILRFFIFFLKTVI
EEEHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA