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The map label for this gene is vacB [H]

Identifier: 71893392

GI number: 71893392

Start: 41677

End: 43755

Strand: Reverse

Name: vacB [H]

Synonym: MHJ_0033

Alternate gene names: 71893392

Gene position: 43755-41677 (Counterclockwise)

Preceding gene: 71893393

Following gene: 71893389

Centisome position: 4.88

GC content: 28.52

Gene sequence:

>2079_bases
ATTATATCGGAAGAGCGCTTAAAAAATTTTCTGCGTCAAGAAAAAACTTTTATTGAAATTGTTCGAAAATTTAACATTCC
TTTCAATTTAAATCACCAGCTAACTAATCAAATTAGTAATCTAATTGAAAATTTTGTTGTTTTTAAAACCCCTGAAGGAA
AATATTATGCTCCTAAATTTCTCAAAACCACATCTGGAATTTTCAGAGCAACCAATTCTGCTTTTGGTTTTGTTGAAGAC
AAAGAAAATCCAATCCAGAAAAATAACGTTTTTATCCCCGGGCGTTTTACAGCGAGCGCCCTTGAAGGTGATGAAGTTAA
AATTAATATTTATGCTGATCGTTTCAAAAGTGATCAGGTATTTGGTATAGTAACCAAAATTATTCAAAGAAATAACAAGT
TTTTAATCGGAAAAGCTTTTAAAAATGGGAAATATTGAGATTTTGAACCTGTAAATTTTAAAGGTGATTTTTTGTTCCGT
TGAAACACAACTGATGATTTAATTAATGATAATTATTATAAGGTAAAAATTATTGACTATCAAAAAAATACTTTAAAAAT
AAGCATAATTCAGAAAATTGGTCATAAAAGCGAGCCATTTTTGTATGTAAAAATTCCAATAATTGAATCTGAAGTTTCTA
ATAAATTTAGTGCTGATGTTCTTGAAGAGGCTGAAAAAATTGAAAAGGAAATTACTAATATTGAAAAAAACAGGACCGAC
CTGCGCGATGAATTAGTTATTACAATCGATGGCGAAGATACAAAAGATTTCGATGATGCAATTTCTGTTAAAAAAACAAA
TGAAGGAAATTTTCTTTTGAAAGTCCATATTGCCGATGTTGCCCATTATGTCAAGCAGGATAGTCCAATTGATGTTGAAG
CCCAACGCCGAGGAACATCAATTTATCTACCTCACACCGTCATTCCAATGTTGCCCGAAGAATTATCAAATGGAATTTGC
TCTTTAAAGCCGAATGTTGACCGATTTACAATAACAATGGAAAGTTTAATTAATAAAAGGGGTGAAAATTTATCAATTAA
AATTTATCCATCAGTAATTAACTCAAAATGGCGACTGACTTACAAAGATGTCAATAATTTTTTTAATGGAAAATCTAGTT
TTAACGATACTAATTTAGAAAAAATGTTGAAAACAGCACAAGAATTAAACACCATTTTATCACTGTTTAAAAAAAATCAA
GGTTATATCGATTTGGCCATAGATGAGATAAAAATTATTTTGGATGAACAAGGTTTTACAGAATCTTTAAAATTAAAAAG
TAGGGGAACCTCAGAAGAATTAATTGAAAATTTTATGATTAGAGCTAATGAAAATGTCTCTGAATTCTTAACAAAAAGGA
AAATTCCAATTTTATACAGGGTTCATAACAAGCCTGATCCTGAAAAAATTATGATTTTCAATCAAATTTTGAAGGTGTTA
GGAGTTAAGAATACTTTAAAATTAAACTCAAATTCAAGGGAATTTGCAAGAAAAATTGATGAAATAAAAACAAAAAATAA
CGATAATTTTTTAAAATATTCAATTTTAAGAACAATGCAAAAAGCAATTTATAGCACGGAAAACATAGGTCATTTTGGTT
TGGCAGCGAATTTTTATTCCCATTTTACAAGCCCAATTCGCCGCTACCCGGATCTAATTTTACATCGAATTATTCGTTTT
TTTATCTTTGAAAAAAACAAAAATTTTGATTTTTTCAACGAAATTTTAACTAAAAATTCCTACTTGACCACGGAACTAGA
GCAAAAAGCCTTTAATTTAGAAAGAAAAATTGTCAATATAAAAAAAACTGAATATGTAAAAAAATTAATCGGTCAAACTT
TTAATGCCCAAATAACTTCAATTATAAAATCTGGTTTTTTTGTTGAAATTAACGGACTTTTTGATGCGATGTTAAGTAAT
AAAAGTCTCCCAAACAACGAAAATGATCCATACCAATTGACAGAAGATGGGTTTTGCATTTATAATAGCAGACGAAGATT
TAAATTGGGCGAATTTATTGATATTAAAATTGAAAGTGTTAATGTTTGAGAGGGTAAAATTAGTGCTATTTTAGCCTAA

Upstream 100 bases:

>100_bases
AACGCGGATTTAAAAAATTCACAAAATGAGCAATGTTTGTTGTGGGTTTTATCTTTCTCGGGCTCGCTTTAGTTGTTAGG
TTATTATAAATATTATGGAA

Downstream 100 bases:

>100_bases
AATTCAGTTTTATCACTTTTTTTTTAAAAAAATTATTAATCTTCCATTTTTGTGCAAAATAAAAAATTTAGATAAGCCAA
GTATACTCGCAAAAAATTCT

Product: VACB-like ribonuclease II

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MISEERLKNFLRQEKTFIEIVRKFNIPFNLNHQLTNQISNLIENFVVFKTPEGKYYAPKFLKTTSGIFRATNSAFGFVED
KENPIQKNNVFIPGRFTASALEGDEVKINIYADRFKSDQVFGIVTKIIQRNNKFLIGKAFKNGKYWDFEPVNFKGDFLFR
WNTTDDLINDNYYKVKIIDYQKNTLKISIIQKIGHKSEPFLYVKIPIIESEVSNKFSADVLEEAEKIEKEITNIEKNRTD
LRDELVITIDGEDTKDFDDAISVKKTNEGNFLLKVHIADVAHYVKQDSPIDVEAQRRGTSIYLPHTVIPMLPEELSNGIC
SLKPNVDRFTITMESLINKRGENLSIKIYPSVINSKWRLTYKDVNNFFNGKSSFNDTNLEKMLKTAQELNTILSLFKKNQ
GYIDLAIDEIKIILDEQGFTESLKLKSRGTSEELIENFMIRANENVSEFLTKRKIPILYRVHNKPDPEKIMIFNQILKVL
GVKNTLKLNSNSREFARKIDEIKTKNNDNFLKYSILRTMQKAIYSTENIGHFGLAANFYSHFTSPIRRYPDLILHRIIRF
FIFEKNKNFDFFNEILTKNSYLTTELEQKAFNLERKIVNIKKTEYVKKLIGQTFNAQITSIIKSGFFVEINGLFDAMLSN
KSLPNNENDPYQLTEDGFCIYNSRRRFKLGEFIDIKIESVNVWEGKISAILA

Sequences:

>Translated_692_residues
MISEERLKNFLRQEKTFIEIVRKFNIPFNLNHQLTNQISNLIENFVVFKTPEGKYYAPKFLKTTSGIFRATNSAFGFVED
KENPIQKNNVFIPGRFTASALEGDEVKINIYADRFKSDQVFGIVTKIIQRNNKFLIGKAFKNGKY*DFEPVNFKGDFLFR
*NTTDDLINDNYYKVKIIDYQKNTLKISIIQKIGHKSEPFLYVKIPIIESEVSNKFSADVLEEAEKIEKEITNIEKNRTD
LRDELVITIDGEDTKDFDDAISVKKTNEGNFLLKVHIADVAHYVKQDSPIDVEAQRRGTSIYLPHTVIPMLPEELSNGIC
SLKPNVDRFTITMESLINKRGENLSIKIYPSVINSKWRLTYKDVNNFFNGKSSFNDTNLEKMLKTAQELNTILSLFKKNQ
GYIDLAIDEIKIILDEQGFTESLKLKSRGTSEELIENFMIRANENVSEFLTKRKIPILYRVHNKPDPEKIMIFNQILKVL
GVKNTLKLNSNSREFARKIDEIKTKNNDNFLKYSILRTMQKAIYSTENIGHFGLAANFYSHFTSPIRRYPDLILHRIIRF
FIFEKNKNFDFFNEILTKNSYLTTELEQKAFNLERKIVNIKKTEYVKKLIGQTFNAQITSIIKSGFFVEINGLFDAMLSN
KSLPNNENDPYQLTEDGFCIYNSRRRFKLGEFIDIKIESVNV*EGKISAILA
>Mature_692_residues
MISEERLKNFLRQEKTFIEIVRKFNIPFNLNHQLTNQISNLIENFVVFKTPEGKYYAPKFLKTTSGIFRATNSAFGFVED
KENPIQKNNVFIPGRFTASALEGDEVKINIYADRFKSDQVFGIVTKIIQRNNKFLIGKAFKNGKY*DFEPVNFKGDFLFR
*NTTDDLINDNYYKVKIIDYQKNTLKISIIQKIGHKSEPFLYVKIPIIESEVSNKFSADVLEEAEKIEKEITNIEKNRTD
LRDELVITIDGEDTKDFDDAISVKKTNEGNFLLKVHIADVAHYVKQDSPIDVEAQRRGTSIYLPHTVIPMLPEELSNGIC
SLKPNVDRFTITMESLINKRGENLSIKIYPSVINSKWRLTYKDVNNFFNGKSSFNDTNLEKMLKTAQELNTILSLFKKNQ
GYIDLAIDEIKIILDEQGFTESLKLKSRGTSEELIENFMIRANENVSEFLTKRKIPILYRVHNKPDPEKIMIFNQILKVL
GVKNTLKLNSNSREFARKIDEIKTKNNDNFLKYSILRTMQKAIYSTENIGHFGLAANFYSHFTSPIRRYPDLILHRIIRF
FIFEKNKNFDFFNEILTKNSYLTTELEQKAFNLERKIVNIKKTEYVKKLIGQTFNAQITSIIKSGFFVEINGLFDAMLSN
KSLPNNENDPYQLTEDGFCIYNSRRRFKLGEFIDIKIESVNV*EGKISAILA

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=384, Percent_Identity=30.46875, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI190014625, Length=384, Percent_Identity=30.46875, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI219521928, Length=363, Percent_Identity=29.2011019283747, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI19115966, Length=363, Percent_Identity=29.2011019283747, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI134288890, Length=398, Percent_Identity=27.6381909547739, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI156105695, Length=392, Percent_Identity=24.7448979591837, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI156105693, Length=392, Percent_Identity=24.7448979591837, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI87082383, Length=649, Percent_Identity=30.2003081664099, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787542, Length=479, Percent_Identity=25.678496868476, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI212645896, Length=348, Percent_Identity=30.1724137931034, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17553506, Length=427, Percent_Identity=27.4004683840749, Blast_Score=138, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324552, Length=352, Percent_Identity=32.1022727272727, Blast_Score=158, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323943, Length=443, Percent_Identity=24.1534988713318, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320499, Length=325, Percent_Identity=23.6923076923077, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24649634, Length=367, Percent_Identity=32.1525885558583, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24654592, Length=431, Percent_Identity=25.5220417633411, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922976, Length=431, Percent_Identity=25.5220417633411, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24654597, Length=431, Percent_Identity=25.5220417633411, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 79914; Mature: 79914

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEERLKNFLRQEKTFIEIVRKFNIPFNLNHQLTNQISNLIENFVVFKTPEGKYYAPKF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEECCHH
LKTTSGIFRATNSAFGFVEDKENPIQKNNVFIPGRFTASALEGDEVKINIYADRFKSDQV
HHHHHHHHEECCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCHH
FGIVTKIIQRNNKFLIGKAFKNGKYDFEPVNFKGDFLFRNTTDDLINDNYYKVKIIDYQK
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHCCCEEEEEEEEECC
NTLKISIIQKIGHKSEPFLYVKIPIIESEVSNKFSADVLEEAEKIEKEITNIEKNRTDLR
CEEEEEEEHHCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DELVITIDGEDTKDFDDAISVKKTNEGNFLLKVHIADVAHYVKQDSPIDVEAQRRGTSIY
CEEEEEECCCCCCCHHHHEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCEEHHCCCCEEE
LPHTVIPMLPEELSNGICSLKPNVDRFTITMESLINKRGENLSIKIYPSVINSKWRLTYK
ECCHHHHCCHHHHCCCEEEECCCCCEEEEEHHHHHHCCCCCEEEEEEHHHHCCCEEEEHH
DVNNFFNGKSSFNDTNLEKMLKTAQELNTILSLFKKNQGYIDLAIDEIKIILDEQGFTES
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCH
LKLKSRGTSEELIENFMIRANENVSEFLTKRKIPILYRVHNKPDPEKIMIFNQILKVLGV
HEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
KNTLKLNSNSREFARKIDEIKTKNNDNFLKYSILRTMQKAIYSTENIGHFGLAANFYSHF
CCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TSPIRRYPDLILHRIIRFFIFEKNKNFDFFNEILTKNSYLTTELEQKAFNLERKIVNIKK
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHH
TEYVKKLIGQTFNAQITSIIKSGFFVEINGLFDAMLSNKSLPNNENDPYQLTEDGFCIYN
HHHHHHHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEC
SRRRFKLGEFIDIKIESVNVEGKISAILA
CCCCEECCCEEEEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MISEERLKNFLRQEKTFIEIVRKFNIPFNLNHQLTNQISNLIENFVVFKTPEGKYYAPKF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEECCHH
LKTTSGIFRATNSAFGFVEDKENPIQKNNVFIPGRFTASALEGDEVKINIYADRFKSDQV
HHHHHHHHEECCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCHH
FGIVTKIIQRNNKFLIGKAFKNGKYDFEPVNFKGDFLFRNTTDDLINDNYYKVKIIDYQK
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHCCCEEEEEEEEECC
NTLKISIIQKIGHKSEPFLYVKIPIIESEVSNKFSADVLEEAEKIEKEITNIEKNRTDLR
CEEEEEEEHHCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DELVITIDGEDTKDFDDAISVKKTNEGNFLLKVHIADVAHYVKQDSPIDVEAQRRGTSIY
CEEEEEECCCCCCCHHHHEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCEEHHCCCCEEE
LPHTVIPMLPEELSNGICSLKPNVDRFTITMESLINKRGENLSIKIYPSVINSKWRLTYK
ECCHHHHCCHHHHCCCEEEECCCCCEEEEEHHHHHHCCCCCEEEEEEHHHHCCCEEEEHH
DVNNFFNGKSSFNDTNLEKMLKTAQELNTILSLFKKNQGYIDLAIDEIKIILDEQGFTES
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCH
LKLKSRGTSEELIENFMIRANENVSEFLTKRKIPILYRVHNKPDPEKIMIFNQILKVLGV
HEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
KNTLKLNSNSREFARKIDEIKTKNNDNFLKYSILRTMQKAIYSTENIGHFGLAANFYSHF
CCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TSPIRRYPDLILHRIIRFFIFEKNKNFDFFNEILTKNSYLTTELEQKAFNLERKIVNIKK
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHH
TEYVKKLIGQTFNAQITSIIKSGFFVEINGLFDAMLSNKSLPNNENDPYQLTEDGFCIYN
HHHHHHHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEC
SRRRFKLGEFIDIKIESVNVEGKISAILA
CCCCEECCCEEEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]