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The map label for this gene is tgt

Identifier: 71892010

GI number: 71892010

Start: 266542

End: 267648

Strand: Direct

Name: tgt

Synonym: BPEN_236

Alternate gene names: 71892010

Gene position: 266542-267648 (Clockwise)

Preceding gene: 71892008

Following gene: 71892011

Centisome position: 33.67

GC content: 33.79

Gene sequence:

>1107_bases
ATGCCATTTCAACTATTACAAACAGATGGTCACGCACGTCTTGGTAGACTGATTTGTAACCAAGGAATAGTAGATACACC
TGCTTTTATGCCAGTAGGGACCTATGGATCCATAAAAACATTAACTGCAAAGGAAGTTGAAGCCAGTGGAACACAAATTA
TTTTAAGTAATACTTTTCATTTATGGTTACGTCCTGGTTTAGACATTATAAAACTACACGGCAGCTTACATAAATTCATG
AGTTGGAATGGCCCTATAATTACAGATTCTGGAGGATTCCAAGTATTTAGTCTAAGTAAAACTCGTACAATCACAAGAAA
AGGAGTAGGCTTTAAAAGCCCTATTGACGGTCATCTTGTGTTTTTAACTCCAGAAAAATCCATAGAAATTCAGCATGATT
TGCAATCCGATATAGTTATGATATTTGATGAATGCATATCTTACCCCAACACTTGGGATTATGTAAAAAAATCAGTAAAC
ATATCTTTAAATTGGGCTGAACGCAGCCGTTTACGTTTCGATACATTACATAATTCAAATATGTTATTTGCTATTATTCA
AGGAGGTATGTATGAAAATTTAAGGGATATGTCAGCAAAAGAATTAATAAATATTGGTTTTGATGGATACGCAATAGGAG
GTTTATCAGTAGGTGAGCCGAAAAAAGAAATGTATCGTATTTTATCTCATATATGCAAATTAATTCCTACAAATAAACCA
CGCTATTTAATGGGTGCTGGGAAACCAGAGGATTTGCTGGAGGCTGTACAGAAAGGTATTGATATGTTTGATTGCGTTAT
ACCAACGCGTAACGCTCGTAATGGATATTTGTTTGTTTCTGATGGAACAATTAAAATTCGTAATGCGCAATACAAAAAGG
ACACTTCTCCGTTAGATGGAAACTGCGATTGTTATACTTGTCAACATTACAGTCGTTCGTATTTGCATCATTTAGATTGT
TGCAAAGAAATTCTTGGTGTGCGTTTAAATACCATTCATAATTTAAGATATTACCAGCGTTTAATGGAGGAATTACGTCA
AGCTATAAAAACAAAATCATTGCAAAACTTTATCGATATTTTTTATAAGCGTGTTAATCGTATTTAA

Upstream 100 bases:

>100_bases
ATATATAAATACAGATTATTATAATAAATACAGTTTATAATTAGTACTTACTATATTTATATAGTAATTAATAAACATTT
CTTTAATTAGGATAACATTT

Downstream 100 bases:

>100_bases
TGTTGAATTTATATTCAGTATTTTTTTAATTAGACCCAATGATTCAAATTATTTATAAAACAGGAAATATACATGAACAT
ATTTACTAATGCTGCTTGGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MPFQLLQTDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFHLWLRPGLDIIKLHGSLHKFM
SWNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLVFLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVN
ISLNWAERSRLRFDTLHNSNMLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKP
RYLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDGNCDCYTCQHYSRSYLHHLDC
CKEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDIFYKRVNRI

Sequences:

>Translated_368_residues
MPFQLLQTDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFHLWLRPGLDIIKLHGSLHKFM
SWNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLVFLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVN
ISLNWAERSRLRFDTLHNSNMLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKP
RYLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDGNCDCYTCQHYSRSYLHHLDC
CKEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDIFYKRVNRI
>Mature_367_residues
PFQLLQTDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFHLWLRPGLDIIKLHGSLHKFMS
WNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLVFLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVNI
SLNWAERSRLRFDTLHNSNMLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKPR
YLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDGNCDCYTCQHYSRSYLHHLDCC
KEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDIFYKRVNRI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=41.7142857142857, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=67.574931880109, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.9889502762431, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=31.8840579710145, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=41.3597733711048, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI21355311, Length=260, Percent_Identity=27.3076923076923, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BLOPB (Q493H3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277740.1
- HSSP:   P28720
- ProteinModelPortal:   Q493H3
- SMR:   Q493H3
- STRING:   Q493H3
- GeneID:   3562770
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_236
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   CBLO291272:BPEN_236-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41983; Mature: 41852

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFQLLQTDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFH
CCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCEEEEECCEE
LWLRPGLDIIKLHGSLHKFMSWNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLV
EEECCCCEEEEHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHHCCCCCCCCCCCEEE
FLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVNISLNWAERSRLRFDTLHNSN
EECCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCEEEEEECCCC
MLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKP
EEEEEECCCHHHHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
RYLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDG
CEEEECCCHHHHHHHHHHCCCEEEHCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCC
NCDCYTCQHYSRSYLHHLDCCKEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDI
CCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYKRVNRI
HHHHHHCC
>Mature Secondary Structure 
PFQLLQTDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFH
CCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCEEEEECCEE
LWLRPGLDIIKLHGSLHKFMSWNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLV
EEECCCCEEEEHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHHHHCCCCCCCCCCCEEE
FLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVNISLNWAERSRLRFDTLHNSN
EECCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCEEEEEECCCC
MLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKP
EEEEEECCCHHHHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
RYLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDG
CEEEECCCHHHHHHHHHHCCCEEEHCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCC
NCDCYTCQHYSRSYLHHLDCCKEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDI
CCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYKRVNRI
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA