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The map label for this gene is ispF

Identifier: 71891955

GI number: 71891955

Start: 219209

End: 219682

Strand: Direct

Name: ispF

Synonym: BPEN_172

Alternate gene names: 71891955

Gene position: 219209-219682 (Clockwise)

Preceding gene: 71891954

Following gene: 71891956

Centisome position: 27.69

GC content: 36.71

Gene sequence:

>474_bases
ATGTTACGTATTGGTTATGGGTTTGATGTGCACCAATTTGGAGGATGTCGACCGCTTACCATTGGGGGCGTGAGTATTCC
CTGTATGAAAGGAATTAAAGCGCATTCTGATGGCGACGTGATGATGCATTCACTAATCGATGCTTTATTGGGAGCTGCCT
GTTGTGGTGATATTGGGTCGATGTTTCCGGATACTGATGCTAAATATAATAATATAGATAGTCGAAAATTGTTACGAATT
GCCTGGGAAAAAATTACCATGCAAGGATTCTTATTAGAAAATATAGATATTACTGTAATCCTACAAATCCCAAAGATAAA
CACATATATTTATCAGATGAAACATCATTTATCTCAAGATTTAAATTGTTCAGTAACTAGTATTAATATAAAAGCTACTA
CAACTGACACACTGGGATATATCGGCCGATCTGAGGGTATTGCGTGTATAGCAGTCGTGTTATTAACACGTTAA

Upstream 100 bases:

>100_bases
GTCGTTCTGATAATATAAAAGTTACTCATCAAAATGATTTAAAATTAGCTAACTTTTATTTATCTAAATTATATAAAAAA
ACTTAACAAGGATTGTAAGC

Downstream 100 bases:

>100_bases
ACATTTATTAAAATAATAAAATACGTATATTTCTATTTCATAGTTCACATATAAATCTATCAATACGATAGATTTATCTA
AGAATTATTTTAATTATTAT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MLRIGYGFDVHQFGGCRPLTIGGVSIPCMKGIKAHSDGDVMMHSLIDALLGAACCGDIGSMFPDTDAKYNNIDSRKLLRI
AWEKITMQGFLLENIDITVILQIPKINTYIYQMKHHLSQDLNCSVTSINIKATTTDTLGYIGRSEGIACIAVVLLTR

Sequences:

>Translated_157_residues
MLRIGYGFDVHQFGGCRPLTIGGVSIPCMKGIKAHSDGDVMMHSLIDALLGAACCGDIGSMFPDTDAKYNNIDSRKLLRI
AWEKITMQGFLLENIDITVILQIPKINTYIYQMKHHLSQDLNCSVTSINIKATTTDTLGYIGRSEGIACIAVVLLTR
>Mature_157_residues
MLRIGYGFDVHQFGGCRPLTIGGVSIPCMKGIKAHSDGDVMMHSLIDALLGAACCGDIGSMFPDTDAKYNNIDSRKLLRI
AWEKITMQGFLLENIDITVILQIPKINTYIYQMKHHLSQDLNCSVTSINIKATTTDTLGYIGRSEGIACIAVVLLTR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=56.4102564102564, Blast_Score=176, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BLOPB (Q493M8)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277685.1
- ProteinModelPortal:   Q493M8
- SMR:   Q493M8
- STRING:   Q493M8
- GeneID:   3563118
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_172
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- BioCyc:   CBLO291272:BPEN_172-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17207; Mature: 17207

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGYGFDVHQFGGCRPLTIGGVSIPCMKGIKAHSDGDVMMHSLIDALLGAACCGDIGS
CEEECCCCCHHHCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MFPDTDAKYNNIDSRKLLRIAWEKITMQGFLLENIDITVILQIPKINTYIYQMKHHLSQD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCC
LNCSVTSINIKATTTDTLGYIGRSEGIACIAVVLLTR
CCCEEEEEEEEEECCHHHHCCCCCCCHHEEHEEECCC
>Mature Secondary Structure
MLRIGYGFDVHQFGGCRPLTIGGVSIPCMKGIKAHSDGDVMMHSLIDALLGAACCGDIGS
CEEECCCCCHHHCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MFPDTDAKYNNIDSRKLLRIAWEKITMQGFLLENIDITVILQIPKINTYIYQMKHHLSQD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCC
LNCSVTSINIKATTTDTLGYIGRSEGIACIAVVLLTR
CCCEEEEEEEEEECCHHHHCCCCCCCHHEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA