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The map label for this gene is ispD

Identifier: 71891954

GI number: 71891954

Start: 218487

End: 219194

Strand: Direct

Name: ispD

Synonym: BPEN_171

Alternate gene names: 71891954

Gene position: 218487-219194 (Clockwise)

Preceding gene: 71891953

Following gene: 71891955

Centisome position: 27.6

GC content: 32.91

Gene sequence:

>708_bases
ATGTTGAAAAAAAAAATTCCATACATTACTGCTATCCTTCCAGCAGCAGGCACTGGGAAACGCATGAAAAGTTTATTGCC
GAAACAATACTGCACCATAGGTGATAAAACTTTGATTGAACATTCCATGAACGCCTTGTTATGTCAATCCTGCATTCGTC
ATTGCATCGTAGTAATTAATGCGCGTGATCATTGGTTTCGTCAATTATCTATTTCTTACGATCCTCGAGTTAGTGTAGTT
GTTGGAGGACACACTCGAGCTGATTCAGTTATGGCTGGATTGCAGCATGTAAAAAAATCAGTTTGGGTTATTGTGCATGA
CGCGGTGCGTCCTTGTTTACATCATGAAGATTTATTACGGCTTTTTGAGATCACAAAATTTTCTCAAGTAGGTGGAATTT
TAGCAATACCAATTTGTAATACTATTAAACGAACTTACTGTGGGACTAATTTTATAAGATATACTGTAAATCGTGAAAAT
TTATGGCATGCTTTAACTCCACAGTTGTTTAATTATGATCTTTTAAAATATTGTCTTAAAAAAGCTTTAAAAAATAGAGT
AACTGTTACTGATGAAGCATCAGCTCTAGAATATTGTGGGTATAAATCCATTGTGATACACGGTCGTTCTGATAATATAA
AAGTTACTCATCAAAATGATTTAAAATTAGCTAACTTTTATTTATCTAAATTATATAAAAAAACTTAA

Upstream 100 bases:

>100_bases
GTGAAACTTTTTATCTAATAACATTGTAACAGCAATATTAATTAAATAAAAGTCAACGATGGTTTAATTATTTTGATATA
TGTGGGTGTTTATACTAAAT

Downstream 100 bases:

>100_bases
CAAGGATTGTAAGCATGTTACGTATTGGTTATGGGTTTGATGTGCACCAATTTGGAGGATGTCGACCGCTTACCATTGGG
GGCGTGAGTATTCCCTGTAT

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLKKKIPYITAILPAAGTGKRMKSLLPKQYCTIGDKTLIEHSMNALLCQSCIRHCIVVINARDHWFRQLSISYDPRVSVV
VGGHTRADSVMAGLQHVKKSVWVIVHDAVRPCLHHEDLLRLFEITKFSQVGGILAIPICNTIKRTYCGTNFIRYTVNREN
LWHALTPQLFNYDLLKYCLKKALKNRVTVTDEASALEYCGYKSIVIHGRSDNIKVTHQNDLKLANFYLSKLYKKT

Sequences:

>Translated_235_residues
MLKKKIPYITAILPAAGTGKRMKSLLPKQYCTIGDKTLIEHSMNALLCQSCIRHCIVVINARDHWFRQLSISYDPRVSVV
VGGHTRADSVMAGLQHVKKSVWVIVHDAVRPCLHHEDLLRLFEITKFSQVGGILAIPICNTIKRTYCGTNFIRYTVNREN
LWHALTPQLFNYDLLKYCLKKALKNRVTVTDEASALEYCGYKSIVIHGRSDNIKVTHQNDLKLANFYLSKLYKKT
>Mature_235_residues
MLKKKIPYITAILPAAGTGKRMKSLLPKQYCTIGDKTLIEHSMNALLCQSCIRHCIVVINARDHWFRQLSISYDPRVSVV
VGGHTRADSVMAGLQHVKKSVWVIVHDAVRPCLHHEDLLRLFEITKFSQVGGILAIPICNTIKRTYCGTNFIRYTVNREN
LWHALTPQLFNYDLLKYCLKKALKNRVTVTDEASALEYCGYKSIVIHGRSDNIKVTHQNDLKLANFYLSKLYKKT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=27.8260869565217, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=50.4504504504504, Blast_Score=248, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_BLOPB (Q494E8)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277684.1
- ProteinModelPortal:   Q494E8
- SMR:   Q494E8
- STRING:   Q494E8
- GeneID:   3563117
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_171
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- BioCyc:   CBLO291272:BPEN_171-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26844; Mature: 26844

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKKIPYITAILPAAGTGKRMKSLLPKQYCTIGDKTLIEHSMNALLCQSCIRHCIVVIN
CCCCCCCHHEEEECCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEC
ARDHWFRQLSISYDPRVSVVVGGHTRADSVMAGLQHVKKSVWVIVHDAVRPCLHHEDLLR
CCHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCHHHHHH
LFEITKFSQVGGILAIPICNTIKRTYCGTNFIRYTVNRENLWHALTPQLFNYDLLKYCLK
HHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHCHHHCCHHHHHHHHH
KALKNRVTVTDEASALEYCGYKSIVIHGRSDNIKVTHQNDLKLANFYLSKLYKKT
HHHHCCEEECCCHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKKKIPYITAILPAAGTGKRMKSLLPKQYCTIGDKTLIEHSMNALLCQSCIRHCIVVIN
CCCCCCCHHEEEECCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEC
ARDHWFRQLSISYDPRVSVVVGGHTRADSVMAGLQHVKKSVWVIVHDAVRPCLHHEDLLR
CCHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCHHHHHH
LFEITKFSQVGGILAIPICNTIKRTYCGTNFIRYTVNRENLWHALTPQLFNYDLLKYCLK
HHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHCHHHCCHHHHHHHHH
KALKNRVTVTDEASALEYCGYKSIVIHGRSDNIKVTHQNDLKLANFYLSKLYKKT
HHHHCCEEECCCHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA